Colon

Species	Cells	Enzyme(s)	Medium	Reference
Guinea-Pig Guinea-Pig, male, 200-250g	Myenteric ganglia	Collagenase Type 4 : 0.2% Protease : 0.1%	Kreb's solution	Kang, M. , Maguma, H. , Smith, T. , Ross, G. , Dewey, W. and Akbarali, H. The Role of Î²-arrestin2 in the Mechanism of Morphine Tolerance in the Mouse and Guinea Pig Gastrointestinal Tract. , J Pharmacol Exp Ther 340 , 567-76 , 2012
Guinea-Pig Guinea-pig, adult	Enterochromaffin	Trypsin : 0.05% Collagenase Type 1 : 0.1%	DMEM	Raghupathi, R. , Duffield, M. , Zelkas, L. , Meedeniya, A. , Brookes, S. , Sia, T. , Wattchow, D. , Spencer, N. and Keating, D. Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells. , J Physiol 591 , 5959-75 , 2013
Human Human	Colon	Trypsin : 0.1% Collagenase : 0.1%	HBSS	Ali, M. , Anand, S. , Tangella, K. , Ramkumar, D. and Saif, T. Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry. , J Vis Exp , e52532 , 2015
Human Human	Lamina propria mononuclear	Collagenase Type 1 : 0.1% Deoxyribonuclease I : 5 u/ml	HBSS	Moser, A. , Spindelboeck, W. , Strohmaier, H. , Enzinger, C. , Gattringer, T. , Fuchs, S. , Fazekas, F. , Gorkiewicz, G. , Wurm, P. , Hogenauer, C. and Khalil, M. Mucosal Biopsy Shows Immunologic Changes of the Colon in Patients with Early MS , Neurol Neuroimmunol Neuroinflamm 4 , e362 , 2017
Human Human	Colonic endothelial cells	Collagenase Type 2 : 0.25%	HBSS/5%FBS	Wang D. , Lehman R. , Donner D. , Matli M. , Warren R. and Welton M. Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells , Am J Physiol/Gastro 282 , G1088 , 2002
Human Human	Colonocytes	Collagenase : 0.15%	DMEM/F12	Emenaker N, Calaf G, Cox D, Basson M. and Qureshi N Short Chain Fatty Acids Differentially Modulate Cellular Phenotype and c-myc Protein Levels in Primary Human Nonmalignant and Malignant Colonocytes , J Nutr 46 , 96-105 , 2001
Human Human	Cancer stem cell	Collagenase Type 3 : 200 u/ml Deoxyribonuclease I : 100 u/ml	RPMI-1640	Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F. Phenotypic characterization of human colorectal cancer stem cells , Proc Natl Acad Sci U S A 104 , 10158-63 , 2007
Human Human	Lamina propria lymphocytes	Collagenase : 25 u/ml	HBSS	Ueyama H, Kiyohara T, Sawada N, Isozaki K, Kitamura S, Kondo S, Miyagawa J, Kanayama S, Shinomura Y, Ishikawa H, Ohtani T, Nezu R, Nagata S, Matsuzawa Y. High Fas ligand expression on lymphocytes in lesions of ulcerative colitis , Gut 43 , 48-55 , 1998
Human Human	Colonic epithelial	Collagenase Neutral Protease : 0.3% Deoxyribonuclease I : 0.05%	RPMI 1640	Fukushima, K. and Fiocchi, C. Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. , Am J Physiol Gastrointest Liver Physiol 286 , G804-13 , 2004
Human Human	Epithelial and mucosal lymphocytes	Neutral Protease : 0.1% CLSPA : 0.02% Deoxyribonuclease I : 0.01%	RPMI 1640	Hisamatsu, T. , Watanabe, M. , Ogata, H. , Ezaki, T. , Hozawa, S. , Ishii, H. , Kanai, T. and Hibi, T. Interferon-Inducible Gene Family 1-8U Expression in Colitis-Associated Colon Cancer and Severely Inflamed Mucosa in Ulcerative Colitis. , Cancer Res 59 , 5927-31 , 1999
Human Human	Colonic epithelial	Neutral Protease : 1.2 u/ml Collagenase Type 4 : 50 u/ml	HBSS	Gibson, P. , Rosella, O. , Wilson, A. , Mariadason, J. , Rickard, K. , Byron, K. and Barkla, D. Colonic Epithelial Cell Activation and the Paradoxical Effects of Butyrate. , Carcinogenesis 20 , 539 , 1999
Human Human	Colonic epithelial	Collagenase Type 4 : 0.1%	not listed	Huang, E. , Hynes, M. , Zhang, T. , Ginestier, C. , Dontu, G. , Appelman, H. , Fields, J. , Wicha, M. and Boman, B. Aldehyde Dehydrogenase 1 is a Marker for Normal and Malignant Human Colonic Stem Cells (SC) and Tracks SC Overpopulation During Colon Tumorigenesis. , Cancer Res 69 , 3382-9 , 2009
Human Human	Colonic epithelial	Collagenase : 150 u/ml Neutral Protease : 0.04 mg/ml	Basal X media	Roig, A. , Eskiocak, U. , Hight, S. , Kim, S. , Delgado, O. , Souza, R. , Spechler, S. , Wright, W. and Shay, J. Immortalized Epithelial Cells Derived from Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro. , Gastroenterol 138 , 1012 , 2010
Human Human	Colorectal cancer	Collagenase Type 4 : 1% Deoxyribonuclease I : 0.2%	HBSS	Zhou, J. , Belov, L. , Huang, P. , Shin, J. , Solomon, M. , Chapuis, P. , Bokey, L. , Chan, C. , Clarke, C. , Clarke, S. and Christopherson, R. Surface Antigen Profiling of Colorectal Cancer Using Antibody Microarrays With Fluorescence Multiplexing. , J Immunol Methods 355 , 40 , 2010
Human Human	Colon cancer	Collagenase Type 1 : 300 u/ml Hyaluronidase : 100 u/ml	DMEM/F12	Varnat, F. , Duquet, A. , Malerba, M. , Zbinden, M. , Mas, C. , Gervaz, P. and Ruiz i Altaba, A. Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion. , EMBO Mol Med 1 , 338-51 , 2009
Human Human	Enterochromaffin	Trypsin : 0.05% Collagenase Type 1 : 0.1%	DMEM	Raghupathi, R. , Duffield, M. , Zelkas, L. , Meedeniya, A. , Brookes, S. , Sia, T. , Wattchow, D. , Spencer, N. and Keating, D. Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells. , J Physiol 591 , 5959-75 , 2013
Human Human	Colon enteric neural	Neutral Protease : 0.5% Collagenase animal free : 0.1% Hyaluronidase : 0.1% Deoxyribonuclease I : 0.01%	Ham's F-12	Rollo, B. , Zhang, D. , Stamp, L. , Menheniott, T. , Stathopoulos, L. , Denham, M. , Dottori, M. , King, S. , Hutson, J. and Newgreen, D. Enteric Neural Cells from Hirschsprung Disease Patients Form Ganglia in Autologous Aneuronal Colon , Cell Mol Gastroenterol Hepatol 2 , 92 , 2016
Human Human	Gastrointestinal epithelial	Collagenase Type 1 : 0.2%	DMEM/F12	VanDussen, K. , Marinshaw, J. , Shaikh, N. , Miyoshi, H. , Moon, C. , Tarr, P. , Ciorba, M. and Stappenbeck, T. Development of an Enhanced Human Gastrointestinal Epithelial Culture System to Facilitate Patient-Based Assays. , Gut 64 , 911-20 , 2015
Mouse Mouse, 6-8 week	Lamina propria mononuclear cells	Collagenase Type 2 : 0.015% Deoxyribonuclease I : 0.01%	RPMI	Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M. Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA , J Immunol 168(1) , 411 , 2002
Mouse Mouse	Lympocytes	Collagenase/Dispase : 100 u/ml	RPMI 1640	Annacker O, Coombes JL, Malmstrom V, Uhlig HH, Bourne T, Johansson-Lindbom B, Agace WW, Parker CM, Powrie F. Essential role for CD103 in the T cell-mediated regulation of experimental colitis , J Exp Med 202 , 1051 , 2005
Mouse Mouse, 6-8 week	Cancer stem cell	Collagenase Type 3 : 200 u/ml Deoxyribonuclease I : 100 u/ml	RPMI-1640	Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F. Phenotypic characterization of human colorectal cancer stem cells , Proc Natl Acad Sci U S A 104 , 10158-63 , 2007
Mouse Mouse, 6-8 week	Lamina propria lymphocytes	Collagenase Type 1 : 0.2% Deoxyribonuclease I : 0.01%	HBSS	Totsuka T, Kanai T, Uraushihara K, Iiyama R, Yamazaki M, Akiba H, Yagita H, Okumura K, Watanabe M. Therapeutic effect of anti-OX40L and anti-TNF-alpha MAbs in a murine model of chronic colitis , Am J Physiol/Gastro 284 , G595-603 , 2003
Mouse Mouse	Lamina propria mononuclear cells	Collagenase : 0.05% Deoxyribonuclease I : 0.05% Neutral Protease : 0.3%	HBSS	Weigmann Benno, Tubbe Ingrid, Seidel Daniel, Nicolaev Alex, Becker Christoph, Neurath Markus F. Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue , Nat Protoc 2 , 2307-11 , 2007
Mouse Mouse	Dentritic	Collagenase : 300 u/ml Deoxyribonuclease I : 0.002%	RPMI 1640	Abe, K. , Nguyen, K. , Fine, S. , Mo, J. , Shen, C. , Shenouda, S. , Corr, M. , Jung, S. , Lee, J. , Eckmann, L. and Raz, E. Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells. , Proc Natl Acad Sci U S A 104 , 17022 , 2007
Mouse Mouse	Lamina propria	Collagenase Type 2 : 0.1% Neutral Protease : 0.1% Deoxyribonuclease I : 0.004%	RPMI 1640	Atarashi, K. , Nishimura, J. , Shima, T. , Umesaki, Y. , Yamamoto, M. , Onoue, M. , Yagita, H. , Ishii, N. , Evans, R. , Honda, K. and Takeda, K. ATP Drives Lamina Propria T(H)17 Cell Differentiation. , Nature 455 , 808 , 2008
Mouse Mouse	Lamina propria	Collagenase : 100 u/ml Deoxyribonuclease I : 0.004%	RPMI 1640	Larmonier, C. , Shehab, K. , Laubitz, D. , Jamwal, D. , Ghishan, F. and Kiela, P. Transcriptional Reprogramming and Resistance to Colonic Mucosal Injury in Poly(ADP-ribose) Polymerase 1 (PARP1)-deficient Mice. , J Biol Chem 291 , 8918-30 , 2016
Mouse Mouse, 8-10 week	Lamina propria lymphocytes	Neutral Protease : 3 u/ml Collagenase Type 4 : 0.05%	HBSS	Round, J. and Mazmanian, S. Inducible Foxp3+ Regulatory T-cell Development by a Commensal Bacterium of the Intestinal Microbiota. , Proc Natl Acad Sci U S A 107 , 12204-9 , 2010
Mouse Mouse	Colon organoid	Collagenase Type 1 : 800 u/ml Neutral Protease : 0.013%	DMEM	Fattahi, F. , Steinbeck, J. , Kriks, S. , Tchieu, J. , Zimmer, B. , Kishinevsky, S. , Zeltner, N. , Mica, Y. , El-Nachef, W. , Zhao, H. , de Stanchina, E. , Gershon, M. , Grikscheit, T. , Chen, S. and Studer, L. Deriving Human ENS Lineages for Cell Therapy and Drug Discovery in Hirschsprung Disease. , Nature 531 , 105-9 , 2016
Rat Rat, adult	Colon smooth muscle	Soybean Trypsin Inhibitor : 0.01% Collagenase Type 2 : 0.1%	DMEM	Somara, S. , Bashllari, D. , Gilmont, R. and Bitar, K. Real-Time Dynamic Movement of Caveolin-1 During Smooth Muscle Contraction of Human Colon and Aged Rat Colon Transfected with Caveolin-1 cDNA. , Am J Physiol Gastrointest Liver Physiol 300 , G1022 , 2011