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Tissue References Search Tool

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Colon
Human
Human
Wang D. , Lehman R. , Donner D. , Matli M. , Warren R. and Welton M.
Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells ,
Am J Physiol/Gastro
 282 , G1088 , 2002
Cells
Colonic endothelial cells
Enzymes
Medium
HBSS/5%FBS
Colon
Human
Human
Emenaker N, Calaf G, Cox D, Basson M. and Qureshi N
Short Chain Fatty Acids Differentially Modulate Cellular Phenotype and c-myc Protein Levels in Primary Human Nonmalignant and Malignant Colonocytes ,
J Nutr
 46 , 96-105 , 2001
Cells
Colonocytes
Enzymes
Collagenase : 0.15%
Medium
DMEM/F12
Colon
Human
Human
Hisamatsu, T. , Watanabe, M. , Ogata, H. , Ezaki, T. , Hozawa, S. , Ishii, H. , Kanai, T. and Hibi, T.
Interferon-Inducible Gene Family 1-8U Expression in Colitis-Associated Colon Cancer and Severely Inflamed Mucosa in Ulcerative Colitis. ,
Cancer Res
 59 , 5927-31 , 1999
Cells
Epithelial and mucosal lymphocytes
Enzymes
CLSPA : 0.02%
Medium
RPMI 1640
Colon
Human
Human
Gibson, P. , Rosella, O. , Wilson, A. , Mariadason, J. , Rickard, K. , Byron, K. and Barkla, D.
Colonic Epithelial Cell Activation and the Paradoxical Effects of Butyrate. ,
Carcinogenesis
 20 , 539 , 1999
Cells
Colonic epithelial
Enzymes
Neutral Protease : 1.2 u/ml
Collagenase Type 4 : 50 u/ml
Medium
HBSS
Colon
Human
Human
Ueyama H, Kiyohara T, Sawada N, Isozaki K, Kitamura S, Kondo S, Miyagawa J, Kanayama S, Shinomura Y, Ishikawa H, Ohtani T, Nezu R, Nagata S, Matsuzawa Y.
High Fas ligand expression on lymphocytes in lesions of ulcerative colitis ,
Gut
 43 , 48-55 , 1998
Cells
Lamina propria lymphocytes
Enzymes
Collagenase : 25 u/ml
Medium
HBSS
Colon
Mouse
Mouse
Larmonier, C. , Shehab, K. , Laubitz, D. , Jamwal, D. , Ghishan, F. and Kiela, P.
Transcriptional Reprogramming and Resistance to Colonic Mucosal Injury in Poly(ADP-ribose) Polymerase 1 (PARP1)-deficient Mice. ,
J Biol Chem
 291 , 8918-30 , 2016
Cells
Lamina propria
Enzymes
Collagenase : 100 u/ml
Medium
RPMI 1640
File
11632.pdf (3.04 MB)
Colon
Mouse
Mouse
Fattahi, F. , Steinbeck, J. , Kriks, S. , Tchieu, J. , Zimmer, B. , Kishinevsky, S. , Zeltner, N. , Mica, Y. , El-Nachef, W. , Zhao, H. , de Stanchina, E. , Gershon, M. , Grikscheit, T. , Chen, S. and Studer, L.
Deriving Human ENS Lineages for Cell Therapy and Drug Discovery in Hirschsprung Disease. ,
Nature
 531 , 105-9 , 2016
Cells
Colon organoid
Enzymes
Collagenase Type 1 : 800 u/ml
Neutral Protease : 0.013%
Medium
DMEM
File
11679.pdf (5.73 MB)
Colon
Mouse
Mouse, 8-10 week
Round, J. and Mazmanian, S.
Inducible Foxp3+ Regulatory T-cell Development by a Commensal Bacterium of the Intestinal Microbiota. ,
Proc Natl Acad Sci U S A
 107 , 12204-9 , 2010
Cells
Lamina propria lymphocytes
Enzymes
Medium
HBSS
File
11634.pdf (768.36 KB)
Colon
Mouse
Mouse
Atarashi, K. , Nishimura, J. , Shima, T. , Umesaki, Y. , Yamamoto, M. , Onoue, M. , Yagita, H. , Ishii, N. , Evans, R. , Honda, K. and Takeda, K.
ATP Drives Lamina Propria T(H)17 Cell Differentiation. ,
Nature
 455 , 808 , 2008
Cells
Lamina propria
Enzymes
Medium
RPMI 1640
Colon
Mouse
Mouse, 6-8 week
Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F.
Phenotypic characterization of human colorectal cancer stem cells ,
Proc Natl Acad Sci U S A
 104 , 10158-63 , 2007
Cells
Cancer stem cell
Enzymes
Collagenase Type 3 : 200 u/ml
Deoxyribonuclease I : 100 u/ml
Medium
RPMI-1640
Colon
Mouse
Mouse
Weigmann Benno, Tubbe Ingrid, Seidel Daniel, Nicolaev Alex, Becker Christoph, Neurath Markus F.
Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue ,
Nat Protoc
 2 , 2307-11 , 2007
Cells
Lamina propria mononuclear cells
Enzymes
Medium
HBSS
Colon
Mouse
Mouse
Abe, K. , Nguyen, K. , Fine, S. , Mo, J. , Shen, C. , Shenouda, S. , Corr, M. , Jung, S. , Lee, J. , Eckmann, L. and Raz, E.
Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells. ,
Proc Natl Acad Sci U S A
 104 , 17022 , 2007
Cells
Dentritic
Enzymes
Collagenase : 300 u/ml
Medium
RPMI 1640
Colon
Mouse
Mouse
Annacker O, Coombes JL, Malmstrom V, Uhlig HH, Bourne T, Johansson-Lindbom B, Agace WW, Parker CM, Powrie F.
Essential role for CD103 in the T cell-mediated regulation of experimental colitis ,
J Exp Med
 202 , 1051 , 2005
Cells
Lympocytes
Enzymes
Collagenase/Dispase : 100 u/ml
Medium
RPMI 1640
Colon
Mouse
Mouse, 6-8 week
Totsuka T, Kanai T, Uraushihara K, Iiyama R, Yamazaki M, Akiba H, Yagita H, Okumura K, Watanabe M.
Therapeutic effect of anti-OX40L and anti-TNF-alpha MAbs in a murine model of chronic colitis ,
Am J Physiol/Gastro
 284 , G595-603 , 2003
Cells
Lamina propria lymphocytes
Enzymes
Medium
HBSS
Colon
Mouse
Mouse, 6-8 week
Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.
Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA ,
J Immunol
 168(1) , 411 , 2002
Cells
Lamina propria mononuclear cells
Enzymes
Medium
RPMI
Colon
Rat
Rat, adult
Somara, S. , Bashllari, D. , Gilmont, R. and Bitar, K.
Real-Time Dynamic Movement of Caveolin-1 During Smooth Muscle Contraction of Human Colon and Aged Rat Colon Transfected with Caveolin-1 cDNA. ,
Am J Physiol Gastrointest Liver Physiol
 300 , G1022 , 2011
Cells
Colon smooth muscle
Enzymes
Medium
DMEM
File
10883.pdf (2.2 MB)
Endothelial
Bovine
Bovine
Ricken Albert M, Traenkner Anja, Merkwitz Claudia, Hummitzsch Katja, Grosche Jens, Spanel-Borowski Katharina.
The short prolactin receptor predominates in endothelial cells of micro- and macrovascular origin ,
J Vasc Res
 44 , 19-30 , 2007
Cells
Bovine umbilical cord (BUVEC)
Enzymes
Collagenase : 0.1%
Medium
Dulbecco's/Ham F-12
Endothelial
Bovine
Bovine
Yu, M. , McAndrew, R. , Al-Saghir, R. , Maier, K. , Medhora, M. , Roman, R. and Jacobs, E.
Nitric Oxide Contributes to 20-HETE-Induced Relaxation of Pulmonary Arteries. ,
J Appl Physiol
 93 , 1391 , 2002
Cells
Pulmonary artery endothelial and smooth muscle cells
Enzymes
Collagenase : 0.04-0.05%
Medium
RPMI-1640
File
10723.pdf (401.6 KB)
Endothelial
Bovine
Bovine, (Bos taurus), calf
Del Vecchio, P. , Siflinger-Birnboim, A. , Belloni, P. , Holleran, L. , Lum, H. and Malik, A.
Culture and Characterization of Pulmonary Microvascular Endothelial Cells ,
In Vitro Cell Dev Biol
 28A , 711 , 1992
Cells
Endothelial
Pulmonary
Enzymes
Collagenase : 1000 u/ml
Collagenase Type 2 : 1000 u/ml
Medium
PBS, CMF
Endothelial
Bovine
Calf
Vender, R.
Role of Endothelial Cells in the Proliferative Response of Cultured Pulmonary Vascular Smooth Muscle Cells to Reduced Oxygen Tension ,
In Vitro Cell Dev Biol
 28A , 403 , 1992
Cells
Endothelial
Enzymes
Trypsin : 0.25%
Medium
HEPES
Endothelial
Bovine
Bovine, adult
Machi, T. , Kassell, N.F. and Scheld, W.M.
Isolation and Characterization of Endothelial Cells From Bovine Cerebral Arteries ,
In Vitro Cell Dev Biol
 26 , 291 , 1990
Cells
Cerebral artery
Endothelial
Enzymes
Collagenase : 0.2%
Medium
HBSS
Endothelial
Bovine
Bovine
Martin, T.
Formation of Diacylglycerol by a Phospholipase D-phosphatidate Phosphatase Pathway Specific for Phosphatidylcholine in Endothelial Cells ,
Biochim Biophys Acta
 962 , 282 , 1988
Cells
Endothelial, pulmonary artery
Enzymes
Collagenase : 0.1%
Medium
CMF-Dulbecco's PBS
Endothelial
Bovine
Bovine
DeNucci, G. , Gryglewski, R.J. , Warner, T.D. and Vane, J.R.
Receptor-Mediated Release of Endothelium-Derived Relaxing Factor and Prostacyclin From Bovine Aortic Endothelial Cells Is Coupled ,
Proc Natl Acad Sci U S A
 85 , 2334 , 1988
Cells
Endothelial
Aortic
Enzymes
Trypsin : 0.05%
Medium
Krebs Ringer solution
Endothelial
Bovine
Bovine
Carson, M.P. and Haudenschild, C.C.
Microvascular Endothelium and Pericytes: High Yield, Low Passage Cultures ,
In Vitro Cell Dev Biol
 22 , 344 , 1986
Cells
Endothelial
Aorta
Enzymes
Medium
PBS
Endothelial
Bovine
Bovine
Kinsella, Michael G. and Wight, Thomas N.
Modulation of Sulfated Proteoglycan Synthesis by Bovine Aortic Endothelial Cells During Migration ,
J Cell Biol
 102 , 679 , 1986
Cells
Endothelial
Aortic
Enzymes
Medium
DMEM
Endothelial
Bovine
Bovine
Gospodarowicz, D. , Massoglia, S. , Cheng, J. and Fujii, D.
Effect of Fibroblast Growth Factor and Lipoproteins on the Proliferation of Endothelial Cells Derived From Bovine Adrenal Cortex, Brain Cortex, and Corpus Luteum Capillaries ,
J Cell Physiol
 127 , 121 , 1986
Cells
Endothelial
Enzymes
Collagenase : 0.5%
Medium
DMEM/Ham's F-12
Endothelial
Bovine
Bovine
Goetz, I.. , Warren, J. , Estrada, C. , Roberts, E. and Krause, D.
Long-Term Cultivation of Arterial and Capillary Endothelium From Adult Bovine Brain ,
In Vitro Cell Dev Biol
 21 , 172 , 1985
Cells
Endothelial
Brain arteries
Enzymes
Medium
Dulbecco's PBS
Endothelial
Bovine
Calf
Voyta, J. , Via, D. , Butterfield, C. and Zetter, B.
Identification and Isolation of Endothelial Cells Based on Their Increased Uptake of Acetylated-Low Density Lipoprotein ,
J Cell Biol
 99 , 2034 , 1984
Cells
Endothelial
Smooth muscle
Enzymes
Collagenase : 0.75%
Medium
DMEM
Endothelial
Bovine
Bovine
Scott, D. , Murray, J. and Barnes, M.
Investigation of the Attachment of Bovine Corneal Endothelial Cells ,
Exp Cell Res
 144 , 472 , 1983
Cells
Endothelial
Corneal
Enzymes
Trypsin : 0.2%
Medium
PBS: DMEM
Endothelial
Bovine
Bovine, 2-3 weeks
Lee, S. , Douglas, W. , Deneke, S. and Fanburg, B.
Ultrastructural Changes in Bovine Pulmonary Artery Endothelial Cells Exposed to 80% O2 In Vitro ,
In Vitro
 19 , 714 , 1983
Cells
Endothelial
Pulmonary artery
Enzymes
Medium
RPMI 1640 w/1% Fetal Bovine Serum
Endothelial
Bovine
Bovine
Robinson, J. and Gospodarowicz, D.
Glycosaminoglycans Synthesized by Cultured Bovine Corneal Endothelial Cells ,
J Cell Physiol
 117 , 368 , 1983
Cells
Endothelial, Corneal
Enzymes
Trypsin : 0.05%
Medium
0.01M Phosphate buffer with 0.02% EDTA
0.9% NaCl (See Reference)
Endothelial
Bovine
Bovine
Olander, J. , Marasa, J. , Kimes, R. , Johnston, G. and Feder, J.
An assay measuring the stimulation of several types of bovine endothelial cells by growth factor(s) derived from cultured human tumor cells ,
In Vitro
 18 , 99 , 1982
Cells
Endothelial
Subclavian vein
Enzymes
Medium
PBS
Endothelial
Bovine
Bovine
Makarski, J. S.
Stimulation of cyclic AMP production by vasoactive agents in cultured bovine aortic and pulmonary artery endothelial cells ,
In Vitro
 17 , 450 , 1981
Cells
Aortic
Pulmonary artery
Enzymes
Medium
PBS
Endothelial
Bovine
Calf, fetal, 4-9 months
Rosen, E. , Mueller, S. , Noveral, J. and Levine, E.
Proliferative Characteristics of Clonal Endothelial Cell Strains ,
J Cell Physiol
 107 , 123 , 1981
Cells
Endothelial
Enzymes
Medium
PBS
Endothelial
Bovine
Bovine, adult
Cotta-Pereira, G. , Sage, H. , Bornstein, P. , Ross, R. and Schwartz, S.
Studies of Morphologically Atypical ("Sprouting") Cultures of Bovine Aortic Endothelial Cells. Growth Characteristics and Connective Tissue Protein Synthesis ,
J Cell Physiol
 102 , 183 , 1980
Cells
Aorta
Enzymes
Collagenase Type 1 : 125 u/ml
Medium
Dulbecco's PBS with calcium and magnesium
Endothelial
Bovine
Bovine, young
Ryan, U. , Mortara, M. and Whitaker, C.
Methods for Microcarrier Culture of Bovine Pulmonary Artery Endothelial Cells Avoiding the Use of Enzymes ,
Tissue Cell
 12 , 619 , 1980
Cells
Pulmonary artery
Enzymes
Collagenase : 0.1%
Medium
Medium 199

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.