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Tissue References Search Tool

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Heart
Mouse
Mouse
Chen, H. , Yong, W. , Ren, S. , Shen, W. , He, Y. , Cox, K. , Zhu, W. , Li, W. , Soonpaa, M. , Payne, RM, Franco, D. , Field, L. , Rosen, V. , Wang, Y. and Shou, W.
Overexpression of Bone Morphogenetic Protein 10 in Myocardium Disrupts Cardiac Postnatal Hypertrophic Growth. ,
J Biol Chem
 281 , 27481 , 2006
Cells
Cardiomyocytes
Enzymes
Medium
PBS
Heart
Mouse
Mouse
Lu Tong, Ye Dan, Wang Xiaoli, Seubert John M, Graves Joan P, Bradbury J Alyce, Zeldin Darryl C, Lee Hon-Chi.
Cardiac and vascular KATP channels in rats are activated by endogenous epoxyeicosatrienoic acids through different mechanisms ,
J Physiol
 575 , 627-44 , 2006
Cells
Ventricular myocytes and mesenteric arterial SMC
Enzymes
Papain : 0.175%
Medium
Krebs-Ringer
Heart
Mouse
Mouse
Kobayashi, M. , Inoue, K. , Warabi, E. , Minami, T. and Kodama, T.
A Simple Method of Isolating Mouse Aortic Endothelial Cells. ,
J Atheroscler Thromb
 12 , 138 , 2005
Cells
Aortic endothelial
Enzymes
Medium
DMEM
Heart
Mouse
Mouse, neonatal
Takahashi N, Wang X, Tanabe S, Uramoto H, Jishage K, Uchida S, Sasaki S, Okada Y.
ClC-3-independent sensitivity of apoptosis to Cl- channel blockers in mouse cardiomyocytes ,
Cell Physiol Biochem
 15 , 263 , 2005
Cells
Cardiomyoctes
Enzymes
Collagenase Type 1 : 150 u/ml
Trypsin : 0.01%
Medium
M199
Heart
Mouse
Mouse
Fukumoto Yoshihiro, Deguchi Jun-o, Libby Peter, Rabkin-Aikawa Elena, Sakata Yasuhiko, Chin Michael T, Hill Christopher C, Lawler Patrick R, Varo Nerea, Schoen Frederick J, Krane Stephen M, Aikawa Masanori.
Genetically determined resistance to collagenase action augments interstitial collagen accumulation in atherosclerotic plaques ,
Circulation
 110 , 1953-9 , 2004
Cells
Smooth muscle cells
Enzymes
Elastase : 0.0125%
Medium
DMEM
Heart
Mouse
Mouse, female, 14-18 g
Zhang Sui, Wang Dachun, Estrov Zeev, Raj Sean, Willerson James T, Yeh Edward T H.
Both cell fusion and transdifferentiation account for the transformation of human peripheral blood CD34-positive cells into cardiomyocytes in vivo ,
Circulation
 110 , 3803-7 , 2004
Cells
Cardiomyocytes
Enzymes
Elastase : 0.03%
Pancreatin : 0.06%
Medium
see reference
Heart
Mouse
Mouse, 6-8 week
Qian, Q. , Hunter, L. , Li, M. , Marin-Padilla, M. , Prakash, Y. , Somlo, S. , Harris, P. , Torres, V. and Sieck, G.
Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells ,
Hum Mol Genet
 12(15) , 1875 , 2003
Cells
Vascular smooth muscle
Enzymes
Papain : 10 u/ml
Elastase : .005%
Collagenase : 0.05%
Deoxyribonuclease I : 1000 u/ml
PDS kit : with modifications
Medium
EBSS
Heart
Mouse
Mouse
Rybkin, I. , Markham, D. , Yan, Z. , Bassel-Duby, R. , Williams, R. and Olson, E.
Conditional Expression of SV40 T-antigen in Mouse Cardiomyocytes Facilitates an Inducible Switch from Proliferation to Differentiation ,
J Biol Chem
 278(18) , 15927 , 2003
Cells
Cardiomyocytes
Enzymes
Medium
DMEM/F-12
Heart
Mouse
Mouse, neonatal and adult
Schreiber, K. , Paquet, L. , Allen, B. and Rindt, H.
Protein Kinase C Isoform Expression and Activity in the Mouse Heart ,
Am J Physiol Heart Circ Physiol
 281(5) , H2062 , 2001
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 0.03-0.2%
Medium
Joklik's MEM
Heart
Mouse
Mouse, 1-6 day
Watzka SB, Lucien J, Shimada M, Edwards V, Yeger H, Hannigan G. and Coles JG.
Selection of viable cardiomyocytes for cell transplantation using three-dimensional tissue culture ,
Transplantation
 70 , 1310 , 2000
Cells
Cardiomyocytes
Enzymes
Medium
PBS
Heart
Mouse
Mouse, NTg and Tg, 8 - 12 week
Nelson, D. , Setser, E. , Hall, D. , Schwartz, S. , Hewitt, T. , Klevitsky, R. , Osinska, H. , Bellgrau, D. , Duke, R. and Robbins, J.
Proinflammatory Consequences of Transgenic Fas Ligand Expression in the Heart ,
J Clin Invest
 105 (9) , 1199 , 2000
Cells
Myocytes
Enzymes
Collagenase Type 2 : 150 u/ml
Medium
Joklik's MEM
Heart
Mouse
Mouse
Christensen G, Minamisawa S, Gruber PJ, Wang Y, Chien KR.
High-efficiency, long-term cardiac expression of foreign genes in living mouse embryos and neonates ,
Circulation
 101 , 178-84 , 2000
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 150 u/ml
Medium
Joklik's MEM
Heart
Mouse
Mouse, 2-4 mo
Zhou YY, Wang SQ, Zhu WZ, Chruscinski A, Kobilka BK, Ziman B, Wang S, Lakatta EG, Cheng H, Xiao RP.
Culture and adenoviral infection of adult mouse cardiac myocytes: methods for cellular genetic physiology ,
Am J Physiol Heart Circ Physiol
 279 , H429-36 , 2000
Cells
Ventricular myocytes
Enzymes
Protease XIV : 0.002%
Medium
MEM
Heart
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Heart
Mouse
Mouse (BALB/c), adult
Felzen, B. , Shilkrut, M. , Less, H. , Sarapov, I. , Maor, G. , Coleman, R. , Robinson, R. and et al.
Fas (CD95/Apo-1)-Mediated Damage to Ventricular Myocytes Induced by Cytotoxic T Lymphocytes from Perforin-Deficient Mice ,
Circ Res
 82 , 438 , 1998
Cells
Myocytes
Enzymes
Medium
Tyrode's solution
Heart
Mouse
Mouse, adult
Grupp, I. , Lorenz, J. , Walsh, R. , Boivin, G. and Rindt, H.
Overexpression of Alpha1B-Adrenergic Receptor Induces Left Ventricular Dysfunction in the Absence of Hypertrophy. ,
Am J Physiol
 275 , H1338-50 , 1998
Cells
Cardiomyocytes
Enzymes
Medium
Joklik's MEM
Heart
Mouse
Mouse, P0-P1
Arber, S. , Hunter, J. , Ross, J. , Hongo, M. , Sansig, G. , Borg, J. , Perriard, J. , Chien, K. and Caroni, P.
MLP-Deficient Mice Exhibit a Disruption of Cardiac Cytoarchitectural Organization, Dilated Cadiomyopathy, and Heart Failure ,
Cell
 88 , 393 , 1997
Cells
Cardiomyocytes
Enzymes
Collagenase : 0.045%
Medium
DMEM, Medium 199
Heart
Mouse
Mouse, 6-9 or 21-41 day
Valenzuela, D. , Han, X. , Mende, U. , Fankhauser, C. , Mashimo, H. , Huang, P. , Pfeffer, J. , Neer, E. and Fishman, M.
G alpha(o) is Necessary for Muscarinic Regulation of Ca2+ Channels in Mouse Heart ,
Proc Natl Acad Sci U S A
 94(5) , 1727 , 1997
Cells
Ventricular and atrial myocytes
Enzymes
Collagenase Type 2 : 95 u/ml
Hyaluronidase : 172.5 u/ml
Trypsin : 0.002%
Medium
DMEM/Tyrodes
Heart
Mouse
Mouse
Soonpaa, M. , Kim, K. , Pajak, aL. , Franklin, M. and Field, L.
Cardiomyocyte DNA Synthesis and Binucleation During Murine Development ,
Am J Physiol
 271 , H2183 , 1996
Cells
Cardiomyocyte
Enzymes
Collagenase : 0.17 %
Medium
PBS
Heart
Mouse
Mouse embryos
Wobus, A. , Kleppisch, T. , Maltsev, V. and Hescheler, J.
Cardiomyocyte-Like Cells Differentiated In Vitro From Embryonic Carcinoma Cells P19 are Characterized by Functional Expression of Adrenoceptors and Ca2+ Channels ,
In Vitro Cell Dev Biol
 30A , 425 , 1994
Cells
Myocytes
Enzymes
Medium
DMEM
Heart
Ovine
Ovine
Voigt, N. , Pearman, C. , Dobrev, D. and Dibb, K.
Methods for Isolating Atrial Cells from Large Mammals and Humans. ,
J Mol Cell Cardiol
 86 , 187-98 , 2015
Cells
Atrial myocytes
Enzymes
Protease XIV : 0.006%
Medium
Tyrode's solution
Heart
Ovine
Ovine
Dibb, K. , Clarke, J. , Horn, M. , Richards, M. , Graham, H. , Eisner, D. and Trafford, A.
Characterization of an Extensive Transverse Tubular Network in Sheep Atrial Myocytes and its Depletion in Heart Failure. ,
Circ Heart Fail
 2 , 482-9 , 2009
Cells
Cardiomyocytes
Enzymes
Protease Type XIV : 0.006%
Medium
See reference
Heart
Ovine
Ovine
Dhamoon, A, Pandit, S. , Sarmast, F. , Parisian, K. , Guha, P. , Li, Y. , Bagwe, S. , Taffet, S. and Anumonwo, J.
Unique Kir2.x Properties Determine Regional and Species Differences in the Cardiac Inward Rectifier K+ Current. ,
Circ Res
 94 , 1332-9 , 2004
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 100-200 u/ml
Medium
See reference
Heart
Porcine
Porcine
Butcher, J. and Nerem, R.
Porcine Aortic Valve Interstitial Cells in Three-Dimensional Culture: Comparison of Phenotype with Aortic Smooth Muscle Cells. ,
J Heart Valve Dis
 13 , 478 , 2004
Cells
Aortic smooth muscle and intersitial cells
Enzymes
Collagenase : 600 u/ml
Medium
PBS
Heart
Rabbit
Rabbit, New Zealand, 3-3.5 kg
Fowler, E. , Wang, N. , Hezzell, M. , Chanoit, G. , Hancox, J. and Cannell, M.
Arrhythmogenic Late Ca2+ Sparks in Failing Heart Cells and Their Control by Action Potential Configuration ,
Proc Natl Acad Sci U S A
 117 , 2687-2692 , 2020
Cells
Cardiac Myocytes
Enzymes
Trypsin : 0.08%
Protease XIV : 0.005%
Collagenase Type 1 : 0.1-0.2%
Medium
HEPES
Heart
Rabbit
Rabbit, New Zealand, 2.3-2.9 kg
Farkas, A. , Acsai, K. , Nagy, N. , Toth, A. , Fulop, F. , Seprenyi, G. , Birinyi, P. , Nanasi, P. , Forster, T. , Csanady, M. , Papp, J. , Varrro, A. and Farkas, A.
Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart. ,
Br J Pharmacol
 154 , 93 , 2008
Cells
Cardiomyocytes
Enzymes
Hyaluronidase : see reference
Protease : see reference
Medium
Krebs-Henseleit
Heart
Rabbit
Rabbit
Verheye, S. , Martinet, W. , Kockx, M. , Knaapen, M. , Salu, K. , Timmermans, J. , Ellis, J. , Kilpatrick, D. and DeMayer, G.
Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy ,
J Am Coll Cardiol
 49 , 706 , 2007
Cells
Smooth muscle cells
Enzymes
Collagenase Type 2 : 300 u/ml
Elastase : 5 u/ml
Medium
F10 Ham
Heart
Rabbit
Rabbit, New Zealand, 2 kg
Driesen Ronald B, Dispersyn Gerrit D, Verheyen Fons K, van den Eijnde Stefan M, Hofstra Leo, Thone Fred, Dijkstra Petra, Debie Wiel, Borgers Marcel, Ramaekers Frans C S.
Partial cell fusion: a newly recognized type of communication between dedifferentiating cardiomyocytes and fibroblasts ,
Cardiovasc Res
 68 , 37-46 , 2005
Cells
Cardiomyocytes and fibroblasts
Enzymes
Collagenase : 0.06%
Medium
Medium 199
Heart
Rabbit
Rabbit, New Zealand, white, either sex
Sedarat, F. , Xu, L. , Moore, E. and Tibbits, G.
Colocalization of Dihydropyridine and Ryanodine Receptors in Neonate Rabbit Heart Using Confocal Microscopy ,
Am J Physiol Heart Circ Physiol
 279 , H202 , 2000
Cells
Myocytes
Enzymes
Medium
EGTA-KB
Heart
Rabbit
Rabbit, New Zealand White, 2-3 kg
Spitzer, K. , Ershler, P. , Skolnick, R. and Vaughan-Jones, R.
Generation of Intracellular pH Gradients in Single Cardiac Myocytes with a Microperfusion System ,
Am J Physiol
 278 , H1371 , 2000
Cells
Myocytes
Enzymes
Medium
HEPES
Heart
Rabbit
Rabbit, adult 1.5-2.5 kg
Anumonwo JM, Delmar M. and Jalife J.
Electrophysiology of single heart cells from the rabbit tricuspid valve ,
J Physiol
 425 , 145 , 1990
Cells
Tricuspid valve cells
Enzymes
Collagenase : 0.8 mg/ml
Medium
HEPES Tyrode solution
Heart
Rabbit
Rabbit, New Zealand, white, male, 2-3 kg
Buxton, I. and Brunton, L.
Compartments of Cyclic AMP and Protein Kinase in Mammalian Cardiomyocytes ,
J Biol Chem
 258 (17) , 10233 , 1983
Cells
Cardiomyocytes
Enzymes
Hyaluronidase : 0.5%
Medium
Eagle's MEM
Heart
Rabbit
Rabbit, adult, 2 kg
Dani, A. , Cittadini, A. , Flamini, G. , Festuccia, G. and Terranova, T.
Preparation and Some Properties of Isolated Beating Myocytes from Adult Rabbit Heart ,
J Mol Cell Cardiol
 9 , 777 , 1977
Cells
Myocytes
Enzymes
Hyaluronidase : 0.007%
Medium
Kreb's Ringer
Heart
Rat

Rat, 1-2 day

Yang, M. , Abudureyimu, M. , Wang, X. , Zhou, Y. , Zhang, Y. and Ren, J.
PHB2 ameliorates Doxorubicin-induced cardiomyopathy through interaction with NDUFV2 and restoration of mitochondrial complex I function. , Redox Biol Vol. 65 , 102812 , 2023
Cells
Cardiomyocytes
Enzymes
Medium
DMEM
Heart
Rat
Rat, adult
Zhang, Y. , Wang, Y. , Yanni, J. , Qureshi, M. , Logantha, S. , Kassab, S. , Boyett, M. , Gardiner, N. , Sun, H. , Howarth, F. and Dobrzynski, H.
Electrical Conduction System Remodeling in Streptozotocin-Induced Diabetes Mellitus Rat Heart. ,
Front Physiol
 10 , 826 , 2019
Cells
Sinoatrial nodal
Enzymes
Collagenase Type 4 : 220 u/ml
Elastase : 1.4 u/ml
Protease : 0.45 u/ml
Medium
Tyrode solution
Heart
Rat
Rat, adult
Savi, M. , Bocchi, L. , Mena, P. , Dall'Asta, M. , Crozier, A. , Brighenti, F. , Stilli, D. and Del Rio, D.
In vivo Administration of Urolithin A and B Prevents the Occurrence of Cardiac Dysfunction in Streptozotocin-induced Diabetic Rats. ,
Cardiovasc Diabetol
 16 , 80 , 2017
Cells
Cardiomyocytes
Enzymes
Protease Type XIV : 0.01%
Medium
Tyrode solution

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.