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Epithelial
Chicken
Chick, 5 day old
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Cells
Intestinal mesenchymal and epithelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Epithelial
Fish
Shark (Squalus acanthias)
Karnaky, Jr. , Valentich, J.D. , Currie, M.G. , Oehlenschlager, W.F. and Kennedy, M.P.
Atriopeptin Stimulates Chloride Secretion in Cultured Shark Rectal Gland Cells ,
Am J Physiol
 260 , 1125 , 1991
Cells
Rectal gland
Enzymes
Collagenase : 0.2%
Medium
Ringer's solution
Epithelial
Fish
Shark (Squalus acanthias)
Valentich, J.
Primary Cultures of Shark Rectal Gland Epithelial Cells: A Model for Hormone-Sensitive Chloride Transport ,
J Tiss Cul Meth
 13 , 149 , 1991
Cells
Rectal gland
Enzymes
Collagenase : 0.2%
Medium
Ringer's solution
Epithelial
Frog
Frog, Xenopus laevis, adult, female
Heinke, B. and Clauss, W.
Potassium Conductances in Isolated Single Cells from Xenopus Laevis Colonic Epithelium ,
J Comp Physiol [B]
 169 , 148 , 1999
Cells
Colonic epithelial
Enzymes
Medium
Kreb's
Epithelial
Guinea-Pig
Guinea-pig, Hartley, female, 200 g
Rutten, M.
Use of Commerically Available Cell Culture Inserts for Primary Culture and Electrophysiologic Studies of Guinea Pig Gastric Mucous Epithelial Cells ,
J Tiss Cul Meth
 14 , 235 , 1992
Cells
Epithelial
Enzymes
Medium
DMEM
Epithelial
Guinea-Pig
Guinea-pig, Hartley albino,
500-600 g
Chaminadas, G. , Alkhalaf, M. , Remy-Martin, J.P. , Propper, A.Y. and Adessi, G.L.
Specific Effect of Oestrone Sulphate on Protein Synthesis and Secretion by Cultured Epithelial Cells from Guinea-pig Endometrium ,
J Endocrinol
 123 , 233 , 1989
Cells
Endometrial
Enzymes
Collagenase : 0.25%
Medium
HBSS
Epithelial
Human
Human
Gottipamula, S. , Saraswat, S. and Sridhar, K.
Comparative Study of Isolation, Expansion and Characterization of Epithelial Cells. ,
Cytotherapy
 19 , 263-271 , 2017
Cells
Epithelial
Enzymes
Medium
PBS
Epithelial
Human
Human
Hsueh, Y. , Huang, S. , Lai, J. , Ma, S. , Chen, H. , Wu, S. , Wang, T. , Sun, C. , Ma, K. , Chen, J. , Lai, C. and Ma, D.
Preservation of Epithelial Progenitor Cells from Collagenase-Digested Oral Mucosa During Ex Vivo Cultivation. ,
Sci Rep
 6 , 36266 , 2016
Cells
Oral mucosal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Human
Human
VanDussen, K. , Marinshaw, J. , Shaikh, N. , Miyoshi, H. , Moon, C. , Tarr, P. , Ciorba, M. and Stappenbeck, T.
Development of an Enhanced Human Gastrointestinal Epithelial Culture System to Facilitate Patient-Based Assays. ,
Gut
 64 , 911-20 , 2015
Cells
Gastrointestinal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Human
Human
Fukushima, K. and Fiocchi, C.
Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. ,
Am J Physiol Gastrointest Liver Physiol
 286 , G804-13 , 2004
Cells
Colonic epithelial
Enzymes
Medium
RPMI 1640
Epithelial
Human
Human
Fahlgren, A. , Hammarstrom, S. , Danielsson, A. and Hammarstrom, M.
Increased Expression of Antimicrobial Peptides and Lysozyme in Colonic Epithelial Cells of Patients with Ulcerative Colitis. ,
Clin Exp Immunol
 131 , 90 , 2003
Cells
Intestinal epithelial
Enzymes
Collagenase Type 4 : 72.5 u/ml
Medium
HBSS
Epithelial
Human
Human
Smoot, D. , Sewchand, J. , Young, K. , Desbrodes, B. , Allen, C. and Naab, T.
A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells ,
Meth Cell Sci
 22 , 133 , 2000
Cells
Gastric epithelial cells
Enzymes
Medium
L-15
Epithelial
Human
Human
Sarosiek, J, Marshall, B, Peura, D, Guerrant, L, McCallum, R. and Little, C
The Isolation and Maintenance of Human Gastric Epithelial Cells in Primary Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:10.1 , 1995
Cells
Gastric
Enzymes
Medium
F-12 medium
Epithelial
Human
Human
Emerman, J. and Wilkinson, D.
Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples ,
In Vitro Cell Dev Biol
 26 , 1186 , 1990
Cells
Epithelial
Enzymes
Collagenase : 2.0%
Medium
DMEM/Ham's F-12
Epithelial
Human
Human
Wood, L. and Neufeld, E.
A Cystic Fibrosis Phenotype in Cells Cultured from Sweat Gland Secretory Coil. Altered Kinetics of 36Cl Efflux ,
J Biol Chem
 265 , 12796 , 1990
Cells
Epithelial
Sweat gland
Enzymes
Medium
(see reference)
Epithelial
Mouse
Mouse, male, 8-16 week
Breggia, A. and Himmelfarb, J.
Primary Mouse Renal Tubular Epithelial Cells have Variable Injury Tolerance to Ischemic and Chemical Mediators of Oxidative Stress. ,
Oxid Med Cell Longev
 1 , 33 , 2008
Cells
Renal tubular epithelial
Enzymes
Collagenase : 200 u/ml
Soybean Trypsin Inhibitor : see reference
Medium
HBSS
Epithelial
Mouse
Mouse
Mathew, R. , Degenhard,t K. , Haramaty, L. , Karp, C. and White, E.
Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer. ,
Methods Enzymol
 446 , 77-106 , 2008
Cells
Epithelial
Enzymes
Collagenase : 0.025%
Medium
DMEM
Epithelial
Mouse
Mouse, 6-8 week
Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.
Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA ,
J Immunol
 168(1) , 411 , 2002
Cells
Lamina propria mononuclear cells
Enzymes
Medium
RPMI
Epithelial
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Epithelial
Mouse
Mouse, female
Ishimaru N, Saegusa K, Yanagi K, Haneji N, Saito I, Hayashi Y.
Estrogen deficiency accelerates autoimmune exocrinopathy in murine Sjogren's syndrome through fas-mediated apoptosis ,
Am J Pathol
 155 , 173-81 , 1999
Cells
Salivary gland epithelial
Enzymes
Collagenase Type 1 : 750 u/ml
Hyaluronidase : 500 u/ml
Medium
DMEM/F12
Epithelial
Mouse
Mouse, fetal 12-13 day
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Cells
Intestinal mesenchymal and epithelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Epithelial
Mouse
Mouse
Durban, E.
Submandibular Salivary Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:2.1 , 1995
Cells
Submandibular salivary
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse, (BALB/c), male, 3-5 months
Durban, E.M.
Mouse Submandibular Salivary Epithelial Cell Growth and Differentiation in Long-Term Culture:Influence of the Extracellular Matrix ,
In Vitro Cell Dev Biol
 26 , 33 , 1990
Cells
Epithelial
Submandibular salivary gland
Enzymes
Collagenase Type 3 : 0.16% , 1:1 v/v
Medium
DMEM with 15 mM HEPES
Epithelial
Mouse
Mouse, female
Riser, M. , Huff, B. and Medina, D.
Pepsin Can Be Used To Subculture Viable Mammary Epithelial Cells ,
In Vitro
 19 , 730 , 1983
Cells
Epithelial
Enzymes
Pepsin : 0.1% and 0.05%
Pepsin : 0.1%
Medium
HBSS with 0.2% EDTA, CMF
Epithelial
Mouse
Mouse, (BALB/cfC3H or BALB/c) 8-12 day mid-pregnant
Yang, J. , Flynn, D. , Larson, L. and Hamamoto, S.
Growth in Primary Culture of Mouse Submandibular Epithelial Cells ,
In Vitro
 18 , 435 , 1982
Cells
Epithelial
Submandibular gland
Enzymes
Medium
HBSS
Epithelial
Mouse
Mouse (BALB/cfC3H)
Yang, J. , Guzman, R. , Richards, J. and Nandi, S.
Primary Cultures of Mouse Mammary Tumor Epithelial Cells Embedded in Collagen Gels ,
In Vitro
 16 , 502 , 1980
Cells
Mammary tumors
Epithelial
Enzymes
Collagenase : 1.0%
Medium
HBSS
Epithelial
Mouse
Mouse, C3H, 6-8 weeks
Lillehaug, J. , Mondal, S. and Heidelberger, C.
Establishment of Epithelial Cell Lines from Mouse Regenerating Liver ,
In Vitro
 15 , 910 , 1979
Cells
Epithelial
Enzymes
Collagenase : 0.10%
Medium
DMEM
Epithelial
Porcine
Porcine, 5-60 kg
Wiencke, A. , Kiilgaard, J. , Nicolini, J. , Bundgaard, M. , Ropke, C. and La Cour, M.
Growth of Cultured Porcine Retinal Pigment Epithelial Cells ,
Acta Opthalmol Scand
 81(2) , 170 , 2003
Cells
Retinal pigment epithelial cells
Enzymes
Medium
DMEM
Epithelial
Rabbit
Rabbit, New Zealand white, male, 4-5lb.
Chew, C.S. and Brown,M.R.
Release of Intracellular Ca2+ and Elevation of Inositol Triphosphate by Secretagogues in Parietal and Chief Cells Isolated from Rabbit Gastric Mucosa ,
Biochim Biophys Acta
 888 , 116 , 1986
Cells
Gastric
Parietal and chief
Enzymes
Medium
Sodium phosphate buffer
Epithelial
Rabbit
Rabbit, fetal
Logsdon, C.D. , Bisbee, C.A. , Rutten, M.J. and Machen, T.E.
Fetal Rabbit Gastric Epithelial Cells Cultured on Floating Collagen Gel ,
In Vitro
 18 , 233 , 1981
Cells
Epithelial
Gastric
Enzymes
Medium
HBSS
Epithelial
Rat
Rat
Mei, N. , McDaniel, L. , Dobrovolsky, V. , Guo, X. , Shaddock, J. , Mittelstaedt, R. , Azuma, M. , Shelton, S. , McGarrity, L. , Doerge, D. and Heflich, R.
The Genotoxicity of Acrylamide and Glycidamide in Big Blue Rats. ,
Toxicol Sci
 115 , 412 , 2010
Cells
Mammary epithelial
Enzymes
Medium
HBSS
Epithelial
Rat
Rat, embryonic
Shannon JM, Gebb SA. and Nielsen LD
Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture ,
Development
 126 , 1675 , 1999
Cells
Tracheal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Rat
Rat, fetal, 14-15 day
Simon-Assmann, P. and Kedinger, M
Embryonic Gut-Dissagregated Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12A:3.1 , 1995
Cells
Intestinal mesenchymal and epithelial
Enzymes
Collagenase : 0.03%
Medium
DMEM
Epithelial
Rat
Rat, 4-14 week
Wang N, Koutz CA. and Anderson RE.
A method for the isolation of retinal pigment epithelial cells from adult rats ,
Inv Ophthal Visual Sci
 34(1) , 101 , 1993
Cells
Retinal pigment epithelial cells
Enzymes
Collagenase Type 1 : 65 u/ml
Hyaluronidase : 220 u/ml
Medium
CF Hanks with EDTA
Epithelial
Rat
Rat, 6 day old
Evans GS, Flint N, Somers AS, Eyden B. and Potten CS.
The development of a method for the preparation of rat intestinal epithelial cell primary cultures ,
J Cell Sci
 101 , 219 , 1992
Cells
Rat intestinal epithelial
Enzymes
Collagenase : 300 u/ml
Medium
DMEM
Epithelial
Rat
Rat, SD, adult, 90-120 day old, 350-450 g
Klinefelter, G.
A Novel System for the Co-Culture of Epididymal Epithelial Cells and Sperm From Adult Rats ,
J Tiss Cul Meth
 14 , 195 , 1992
Cells
Epididymal epithelial
Enzymes
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.