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Tissue References Search Tool

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Lung
Rabbit
Rabbit, New Zealand, white
Scott, J.
The Role of Sera, Growth Factors, and Hormones in the In Vitro Production of Disaturated Phosphatidylcholine and Propagation of Undifferentiated Type II Alveolar Cells from the Fetal Rabbit Lung ,
Exp Lung Res
 12 , 181 , 1987
Cells
Alveolar type II
Enzymes
Trypsin : 0.05%
Medium
HBSS
Lung
Rabbit
Rabbit, New Zealand white, adult,male
Finkelstein, J. , Maniscalco, W. and Shapiro, D.
Properties of Freshly Isolated Type II Alveolar Epithelial Cells ,
Biochim Biophys Acta
 762 , 398 , 1983
Cells
Alveolar type II
Enzymes
Trypsin : 0.0025%
Medium
Joklik's MEM
Lung
Rat
Rat
Jones, R. , Capen, D. , Cohen, K. , Munn, L. , Jain, R. and Duda, D.
A Protocol for Phenotypic Detection and Characterization of Vascular Cells of Different Origins in a Lung Neovascularization Model in Rodents. ,
Nat Protoc
 3 , 388-97 , 2008
Cells
Lung
Enzymes
Medium
PBS
Lung
Rat
Rat, SD, 125-150 g
Liebler, J. , Borok, Z. , Li, X. , Zhou, B. , Sandoval, A. and Kim, K.and Crandall, E.
Alveolar Epithelial Type I Cells Express Beta 2-Adrenergic Receptors and G-protein Receptor Kinase 2. ,
J Histochem Cytochem
 52 , 759 , 2004
Cells
Alveolar type I & II
Enzymes
Elastase : 2.5-8 u/ml
Medium
DMEM/F12
Lung
Rat
Rat, SD, 300-400 g
King J, Hamil T, Creighton J, Wu S, Bhat P, McDonald F. and Stevens T
Structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes ,
Microvasc Res
 67 , 139 , 2004
Cells
Pulmonary endothelial cells
Enzymes
Collagenase Type 2 : 1000 u/ml
Medium
DMEM/F-12
Lung
Rat
Rat, Lewis, male, 200-250g
Steinmuller C, Franke-Ullmann G, Lohmann-Matthes ML, Emmendorffer A.
Local activation of nonspecific defense against a respiratory model infection by application of interferon-gamma: comparison between rat alveolar and interstitial lung macrophages ,
Am J Respir Cell Mol Biol
 22 , 481-90 , 2000
Cells
Interstitial lung macrophages
Enzymes
Medium
RPMI-1640
Lung
Rat
Rat, male, 200-250g
Bakhramov, A, Evans, M. and Kozlowski, R
Differential effects of hypoxia on the intracellular Ca2+ concentration of myocytes isolated from different regions of the rat pulmonary arterial tree ,
Exp Physiol
 83 , 337-47 , 1998
Cells
Pulmonary arterial myocytes
Enzymes
Collagenase : 0.15%
Papain : 0.15%
Elastase : 0.05%
Medium
PBS
Lung
Rat
Rat, embryonic day 15
Fraslon-Vanhulle, C, Bourbon, J. and Batenburg, J
Culture of Fetal Alveolar Epithelial Type II Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 13A:2.1 , 1995
Cells
Fetal alveolar epithelial type II
Enzymes
Trypsin : 0.1%
Medium
DMEM
Lung
Rat
Rat, Wistar, female, virgin
Fraslon, C. , Rolland, G. , Bourbon, J. , Rieutort, M. and Valenza, C.
Culture of Fetal Alveolar Epithelial Type II Cells in Serum-Free Medium ,
In Vitro Cell Dev Biol
 27A , 843 , 1991
Cells
Alveolar epithelial type II
Enzymes
Trypsin : 1%
Medium
Eagle's MEM
Lung
Rat
Rat, Wistar
Jassal, D. , Han, R. , Caniggia, I. , Post, M. and Tanswell, A.
Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium ,
In Vitro Cell Dev Biol
 27A , 625 , 1991
Cells
Epithelial
Enzymes
Trypsin : 0.1%
Medium
HBSS
Lung
Rat
Rat, SD, 8 day
Berk, J. , Franzblau, C. and Goldstein, R.
Recombinant Interleukin-1beta Inhibits Elastin Formation by a Neonatal Rat Lung Fibroblast Subtype ,
J Biol Chem
 266 , 3192 , 1991
Cells
Interstitial
Enzymes
Trypsin : 1.125%
Medium
HEPES buffer
Lung
Rat
Rat, Wistar, adult, male and pregnant female (known gestation)
Batenburg, J. , Otto-Verberne, C. , Have-Opbroek, A. and Klazinga, W.
Isolation of Alveolar Type II Cells from Fetal Rat Lung by Differential Adherence in Monolayer Culture ,
Biochim Biophys Acta
 960 , 441 , 1988
Cells
Alveolar type II
Enzymes
Trypsin : 0.1%
Medium
RPMI 1640
Lung
Rat
Rat, SD, male, 150 - 200 g
Brown, L. and Longmore, W.
Altered Phospholipid Secretion in Type II Pneumocytes Isolated from Streptozotocin-diabetic Rats ,
Biochim Biophys Acta
 878 , 258 , 1986
Cells
Pneumocytes type II
Enzymes
Trypsin : 0.30%
Medium
BSS
Lung
Rat
Rat, SD, male
Weller, N. and Karnovksy, M.
Isolation of Pulmonary Alveolar Type I Cells From Adult Rats ,
Am J Pathol
 124 , 448 , 1986
Cells
Alveolar type I
Enzymes
Trypsin : 0.05%
Medium
DMEM
Lung
Rat
Rat, fetus, 19 day
Post, M. , Torday, J. and Smith, B.
Alveolar Type II Cells Isolated from Fetal Rat Lung Organotypic Cultures Synthesize and Secrete Surfactant-Associated Phospholipids and Respond to Fibroblast-Pneumonocyte Factor ,
Exp Lung Res
 7 , 53 , 1984
Cells
Fibroblasts & type II
Enzymes
Trypsin : 0.05%
Medium
HBSS: serum free MEM, CMF
Lung
Rat
Rat, SD, male/female, 180-250 g
Mason, R. , Williams, M. , Greenleaf, R. and Clements, J.
Isolation and Properties of Type II Alveolar Cells from Rat Lung ,
Am Rev Respir Dis
 115 , 1015 , 1977
Cells
Alveolar type II
Enzymes
Trypsin : 0.30%
Medium
BSS
Lung
Rat
Rat, Wistar, pathogen free
King, R.
Metabolic Fate of the Apoproteins of Pulmonary Surfactant ,
Am Rev Respir Dis
 115 , 73 , 1977
Cells
Alveolar type II
Enzymes
Trypsin : 0.50%
Medium
Earle's MEM
Lung
Rat
Rat, fetus, 19 days
Douglas, W. and Teel, R.
An Organotypic in Vitro Model System for Studying Pulmonary Surfactant Production by Type II Alveolar Pneumonocytes ,
Am Rev Respir Dis
 113 , 17 , 1976
Cells
Alveolar pneumonocytes, type II
Enzymes
Trypsin : 0.1 %
Medium
HBSS, CMF
Lung
Rat
Rat, adult
Douglas, W. and Kaighn, M.
Clonal Isolation of Differentiated Rat Lung Cells ,
In Vitro
 10 , 230 , 1974
Cells
Lung
Enzymes
Collagenase : 0.1%
Medium
Moscona saline, CMF
Lung
Rat
Rat, SD, male, 100 g
Kikkawa, Y. and Yoneda, K.
Type II Epithelial Cell of the Lung. I. Method of Isolation ,
Lab Invest
 30 , 76 , 1974
Cells
Alveolar type II
Enzymes
Trypsin : 1.0%
Medium
Joklik's medium
Lymph nodes
Mouse
Mouse
Terhorst, D. , Chelbi, R. , Wohn, C. , Malosse, C. , Tamoutounour, S. , Jorquera, A. , Bajenoff, M. , Dalod, M. , Malissen, B. and Henri, S.
Dynamics and Transcriptomics of Skin Dendritic Cells and Macrophages in an Imiquimod-Induced, Biphasic Mouse Model of Psoriasis. ,
J Immunol
 195 , 4953-61 , 2015
Cells
Dendritic
Enzymes
Medium
RPMI 1640
Lymph nodes
Mouse
Mouse
Dubrot, J. , Duraes, F. , Potin, L. , Capotosti, F. , Brighouse, D. , Suter, T. , LeibundGut-Landmann, S. , Garbi, N. , Reith, W. , Swartz, M. and Hugues, S.
Lymph Node Stromal Cells Acquire Peptide-MHCII Complexes from Dendritic Cells and Induce Antigen-Specific CD4+T Cell Tolerance ,
J Exp Med
 211 , 1153 , 2014
Cells
Stromal
Enzymes
Medium
RPMI
Lymph nodes
Mouse
Mouse
Rayamajhi, M. , Redente, E. , Condon, T. , Gonzalez-Juarrero, M. , Riches, D. and Lenz, L.
Non-Surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry. ,
J Vis Exp
 51 , 2702 , 2011
Cells
Lung draining
Enzymes
Collagenase Type 4 : 0.1-0.125%
Medium
HBSS
Lymph nodes
Mouse
Mouse
Kapasi ZF, Qin D, Kerr WG, Kosco-Vilbois MH, Shultz LD, Tew JG, Szakal AK.
Follicular dendritic cell (FDC) precursors in primary lymphoid tissues ,
J Immunol
 160 , 1078-84 , 1998
Cells
Follicular dentritic
Enzymes
Medium
HBSS
Mammary
Bovine
Bovine, lactating Holstein
Miranda, S. , Wang, Y. , Purdie, N. , Osborne, V. , Coomber, B. and Cant, J.
Selenomethionine Stimulates Expression of Glutathione Peroxidase 1 and 3 and Growth of Bovine Mammary Epithelial Cells in Primary Culture. ,
J Dairy Sci
 92 , 2670 , 2009
Cells
Mammary epithelial
Enzymes
Collagenase Type 3 : 400 u/ml
Hyaluronidase : 100 u/ml
Medium
HBSS
Mammary
Bovine
Bovine, heifer, 200kg
Weber M. , Purup S. , Vestergaard M. , Ellis S. , Scndergard-Andersen J. , Akers R. and Sejrsen K.
Contribution of Insulin-like Growth Factor (IGF)-I and IGF-binding Protein-3 to Mitogenic Activity in Bovine Mammary Extracts and Serum ,
J Endocrinol
 161 , 365 , 1999
Cells
Mammary epithelial
Enzymes
Medium
M-199
Mammary
Bovine
Bovine, young, lactating, female
Anderson, C. and Larson, B.
Comparative Maintenance of Function in Dispersed Cell and Organ Cultures ,
Exp Cell Res
 61 , 24 , 1970
Cells
Mammary
Enzymes
Collagenase : 0.30 %
Medium
HBSS or EBSS
Mammary
Bovine
Bovine, dairy, purebred,
(also rat, Holtzmann, albino, white)
Schingoethe, D. , Hageman, E. and Larson, B.
Essential Amino Acids for Milk Protein Synthesis in the In Vitro Secretory Cell and Stimulation by Elevated Levels ,
Biochim Biophys Acta
 148 , 469 , 1967
Cells
Secretory
Mammary gland
Enzymes
Collagenase : 0.02 - 0.03%
Medium
HBSS or EBSS
Mammary
Canine

Canine, adult

Petrouškova, P. , Hudáková, N. , Almášiová, V. , Valenčáková, A. , Horňáková, L. , Huniadi, M. and Čížková, D.
Establishment of Primary Cell Cultures from Canine Mammary Gland Malignant Tumours: A Preliminary Study. , J Vet Res Vol. 69 , 159-168 , 2025
Cells
Mammary gland tumor
Enzymes
Medium
EBSS
Mammary
Goat
Goat, lactating, 1 month
Blanco, A. , Rife, U. and Larson, B.
Lactate Dehydrogenase Isozymes during Dedifferentiation in Cultures of Mammary Secretory Cells ,
Nature
 214 , 1331 , 1967
Cells
Mammary gland
Enzymes
Collagenase : 0.02 - 0.03%
Medium
HBSS or EBSS
Mammary
Human
Human
Labarge, M. , Garbe, J. and Stampfer, M.
Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture. ,
J Vis Exp
 71 , 2013
Cells
Mammary epithelial
Enzymes
Collagenase : 200 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM/F-12
Mammary
Human
Human, normal biopsy
Ronnov-Jessen L. , Villadsen R. , Edwards J. and Petersen O.
Differential Expression of a Chloride Intracellular Channel Gene, CLIC4, in Transforming Growth Factor-beta1-mediated Conversion of Fibroblasts to Myofibroblasts ,
Am J Pathol
 161 , 471 , 2002
Cells
Fibroblasts
Enzymes
Collagenase Type 3 : 900 u/ml
Medium
DMEM/F-12
Mammary
Human
Human
Huss, F. and Kratz, G.
Mammary Epithelial Cell and Adipocyte Co-Culture in a 3-D Matrix: The First Step Towards Tissue-Engineered Human Breast Tissue. ,
Cells Tissues Organs
 169 , 361-7 , 2001
Cells
Mammary epithelial cells
Enzymes
Medium
DMEM
Mammary
Human
Human
Ogmundsdottir, H. , Petursdottir, I. , Gudmundsdottir, I. , Amundadottir, L. , Ronnov-Jessen, L. and Petersen, O.
Effects of Lymphocytes and Fibroblasts on the Growth of Human Mammary Carcinoma Cells Studied in Short-Term Primary Cultures ,
In Vitro Cell Dev Biol
 29A , 936 , 1993
Cells
Epithelial
Fibroblasts
Enzymes
Collagenase Type 1 : 450 IU/ml
Medium
DMEM/Ham's F-12
Mammary
Human
Human
Ronnov-Jessen, L. , VanDeurs, B. , Nielsen, M. and Petersen, O.W.
Identification, Paracrine Generation, and Possible Function of Human Breast Carcinoma Myofibroblasts in Culture ,
In Vitro Cell Dev Biol
 28 , 273 , 1992
Cells
Myofibroblasts
Enzymes
Collagenase : 900 IU/ml
Medium
DME - F12
Mammary
Human
Human
Emerman, J. and Wilkinson, D.
Routine Culturing of Normal, Dysplastic and Malignant Human Mammary Epithelial Cells from Small Tissue Samples ,
In Vitro Cell Dev Biol
 26 , 1186 , 1990
Cells
Epithelial
Enzymes
Collagenase : 2.0%
Medium
DMEM/Ham's F-12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.