Tissue References Search Tool

Human

Moser, A. , Spindelboeck, W. , Strohmaier, H. , Enzinger, C. , Gattringer, T. , Fuchs, S. , Fazekas, F. , Gorkiewicz, G. , Wurm, P. , Hogenauer, C. and Khalil, M.

Mucosal Biopsy Shows Immunologic Changes of the Colon in Patients with Early MS ,
Neurol Neuroimmunol Neuroinflamm
4 , e362 , 2017

Cells

Lamina propria mononuclear

Enzymes

Collagenase Type 1 : 0.1%

Deoxyribonuclease I : 5 u/ml

Medium

HBSS

Colon

Human

Rollo, B. , Zhang, D. , Stamp, L. , Menheniott, T. , Stathopoulos, L. , Denham, M. , Dottori, M. , King, S. , Hutson, J. and Newgreen, D.

Enteric Neural Cells from Hirschsprung Disease Patients Form Ganglia in Autologous Aneuronal Colon ,
Cell Mol Gastroenterol Hepatol
2 , 92 , 2016

Cells

Colon enteric neural

Enzymes

Neutral Protease : 0.5%

Collagenase animal free : 0.1%

Hyaluronidase : 0.1%

Deoxyribonuclease I : 0.01%

Medium

Ham's F-12

Colon

Human

Ali, M. , Anand, S. , Tangella, K. , Ramkumar, D. and Saif, T.

Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry. ,
J Vis Exp
, e52532 , 2015

Cells

Colon

Enzymes

Trypsin : 0.1%

Collagenase : 0.1%

Medium

HBSS

Colon

Human

VanDussen, K. , Marinshaw, J. , Shaikh, N. , Miyoshi, H. , Moon, C. , Tarr, P. , Ciorba, M. and Stappenbeck, T.

Development of an Enhanced Human Gastrointestinal Epithelial Culture System to Facilitate Patient-Based Assays. ,
Gut
64 , 911-20 , 2015

Cells

Gastrointestinal epithelial

Enzymes

Collagenase Type 1 : 0.2%

Medium

DMEM/F12

Colon

Human

Raghupathi, R. , Duffield, M. , Zelkas, L. , Meedeniya, A. , Brookes, S. , Sia, T. , Wattchow, D. , Spencer, N. and Keating, D.

Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells. ,
J Physiol
591 , 5959-75 , 2013

Cells

Enterochromaffin

Enzymes

Trypsin : 0.05%

Collagenase Type 1 : 0.1%

Medium

DMEM

Colon

Human

Roig, A. , Eskiocak, U. , Hight, S. , Kim, S. , Delgado, O. , Souza, R. , Spechler, S. , Wright, W. and Shay, J.

Immortalized Epithelial Cells Derived from Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro. ,
Gastroenterol
138 , 1012 , 2010

Cells

Colonic epithelial

Enzymes

Collagenase : 150 u/ml

Neutral Protease : 0.04 mg/ml

Medium

Basal X media

Colon

Human

Zhou, J. , Belov, L. , Huang, P. , Shin, J. , Solomon, M. , Chapuis, P. , Bokey, L. , Chan, C. , Clarke, C. , Clarke, S. and Christopherson, R.

Surface Antigen Profiling of Colorectal Cancer Using Antibody Microarrays With Fluorescence Multiplexing. ,
J Immunol Methods
355 , 40 , 2010

Cells

Colorectal cancer

Enzymes

Collagenase Type 4 : 1%

Deoxyribonuclease I : 0.2%

Medium

HBSS

Colon

Human

Huang, E. , Hynes, M. , Zhang, T. , Ginestier, C. , Dontu, G. , Appelman, H. , Fields, J. , Wicha, M. and Boman, B.

Aldehyde Dehydrogenase 1 is a Marker for Normal and Malignant Human Colonic Stem Cells (SC) and Tracks SC Overpopulation During Colon Tumorigenesis. ,
Cancer Res
69 , 3382-9 , 2009

Cells

Colonic epithelial

Enzymes

Collagenase Type 4 : 0.1%

Medium

not listed

Colon

Human

Varnat, F. , Duquet, A. , Malerba, M. , Zbinden, M. , Mas, C. , Gervaz, P. and Ruiz i Altaba, A.

Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion. ,
EMBO Mol Med
1 , 338-51 , 2009

Cells

Colon cancer

Enzymes

Collagenase Type 1 : 300 u/ml

Hyaluronidase : 100 u/ml

Medium

DMEM/F12

Colon

Human

Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F.

Phenotypic characterization of human colorectal cancer stem cells ,
Proc Natl Acad Sci U S A
104 , 10158-63 , 2007

Cells

Cancer stem cell

Enzymes

Collagenase Type 3 : 200 u/ml

Deoxyribonuclease I : 100 u/ml

Medium

RPMI-1640

Colon

Human

Fukushima, K. and Fiocchi, C.

Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. ,
Am J Physiol Gastrointest Liver Physiol
286 , G804-13 , 2004

Cells

Colonic epithelial

Enzymes

Neutral Protease : 0.3%

Deoxyribonuclease I : 0.05%

Medium

RPMI 1640

Colon

Human

Wang D. , Lehman R. , Donner D. , Matli M. , Warren R. and Welton M.

Expression and Endocytosis of VEGF and Its Receptors in Human Colonic Vascular Endothelial Cells ,
Am J Physiol/Gastro
282 , G1088 , 2002

Cells

Colonic endothelial cells

Enzymes

Collagenase Type 2 : 0.25%

Medium

HBSS/5%FBS

Colon

Human

Emenaker N, Calaf G, Cox D, Basson M. and Qureshi N

Short Chain Fatty Acids Differentially Modulate Cellular Phenotype and c-myc Protein Levels in Primary Human Nonmalignant and Malignant Colonocytes ,
J Nutr
46 , 96-105 , 2001

Cells

Colonocytes

Enzymes

Collagenase : 0.15%

Medium

DMEM/F12

Colon

Human

Hisamatsu, T. , Watanabe, M. , Ogata, H. , Ezaki, T. , Hozawa, S. , Ishii, H. , Kanai, T. and Hibi, T.

Interferon-Inducible Gene Family 1-8U Expression in Colitis-Associated Colon Cancer and Severely Inflamed Mucosa in Ulcerative Colitis. ,
Cancer Res
59 , 5927-31 , 1999

Cells

Epithelial and mucosal lymphocytes

Enzymes

Neutral Protease : 0.1%

CLSPA : 0.02%

Deoxyribonuclease I : 0.01%

Medium

RPMI 1640

Colon

Human

Gibson, P. , Rosella, O. , Wilson, A. , Mariadason, J. , Rickard, K. , Byron, K. and Barkla, D.

Colonic Epithelial Cell Activation and the Paradoxical Effects of Butyrate. ,
Carcinogenesis
20 , 539 , 1999

Cells

Colonic epithelial

Enzymes

Neutral Protease : 1.2 u/ml

Collagenase Type 4 : 50 u/ml

Medium

HBSS

Colon

Human

Ueyama H, Kiyohara T, Sawada N, Isozaki K, Kitamura S, Kondo S, Miyagawa J, Kanayama S, Shinomura Y, Ishikawa H, Ohtani T, Nezu R, Nagata S, Matsuzawa Y.

High Fas ligand expression on lymphocytes in lesions of ulcerative colitis ,
Gut
43 , 48-55 , 1998

Cells

Lamina propria lymphocytes

Enzymes

Collagenase : 25 u/ml

Medium

HBSS