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Tissue References Search Tool

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Heart
Rat
Rat, adult
Ren J. and Wold LE.
Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging ,
Biol Proced Online
 3 , 43 , 2001
Cells
Ventricular myocytes
Enzymes
Collagenase : 223 u/ml
Hyaluronidase : 0.01%
Trypsin : 0.002%
Medium
Tyrode solution
Heart
Rat
Rat, SD, male
Dai L, Brookes PS, Darley-Usmar VM. and Anderson PG.
Bioenergetics in cardiac hypertrophy: mitochondrial respiration as a pathological target of NO ,
Am J Physiol/Heart
 281 , H2261-H2269 , 2001
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 140 u/ml
Medium
Krebs-Henseleit
Heart
Rat
Rat, Wistar, female
Pyle, W, Lester, J. and Hofmann, P.
Effects of Kappa-opioid receptor activation of myocardium ,
Am J Physiol Heart Circ Physiol
 281 , H669 , 2001
Cells
Ventricular myocytes
Enzymes
Medium
Ringer solution
Heart
Rat
Rat, SD, 1-2 day
Arutunyan A, Webster D, Swift L, Sarvazyan N.
Localized injury in cardiomyocyte network: a new experimental model of ischemia-reperfusion arrhythmias ,
Am J Physiol Heart Circ Physiol
 280 , H1905-15 , 2001
Cells
Cardiomyocytes
Enzymes
Trypsin : 0.01%
Medium
HBSS
Heart
Rat
Rat, SD, adult female
Puri S, Bansal DD, Uskokovic MR. and MacGregor RR.
Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)(2)D(3) ,
Am J Physiol/Endo
 278(2) , E293 , 2000
Cells
Heart microvascular cells
Enzymes
CLSPA : 250u/ml
Papain : 5 u/ml
Elastase : 0.8 u/ml
Medium
L-15
Heart
Rat
Rat, 1-2 day
Bueno OF, De Windt LJ, Tymitz KM, Witt SA, Kimball TR, Klevitsky R, Hewett TE, Jones SP, Lefer DJ, Peng CF, Kitsis RN. and Molkentin JD.
The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice ,
EMBO J
 19(23) , 6341 , 2000
Cells
Cardiomyocytes
Enzymes
Collagenase Type 1 : 84 u/ml
Pancreatin : 0.05%
Medium
M199 medium
Heart
Rat
Rat, Winstar, 1-2 day
Maki T, Horio T, Yoshihara F, Suga S, Takeo S, Matsuo H. and Kangawa K
Effect of neutral endopeptidase inhibitor on endogenous atrial natriuretic peptide as a paracrine factor in cultured cardiac fibroblasts ,
Br J Pharmacol
 131(6) , 1204 , 2000
Cells
Ventricular myocytes
Enzymes
Medium
DMEM
Heart
Rat
Rat, SD, male, 250-300g
Sun L, Chang J, Kirchhoff SR. and Knowlton AA.
Activation of HSF and selective increase in heat-shock proteins by acute dexamethasone treatment ,
Am J Physiol Heart Circ Physiol
 278(4) , H1091 , 2000
Cells
Cardiomyocytes
Enzymes
Medium
M199
Heart
Rat
Rat, 1-3 day
Adderley SR. and Fitzgerald DJ.
Oxidative damage of cardiomyocytes is limited by extracellular regulated kinases 1/2-mediated induction of cyclooxygenase-2 ,
J Biol Chem
 274 , 5038 , 1999
Cells
Neonatal rat cardiomyocytes
Enzymes
Pancreatin : 0.06%
Medium
DMEM/F12
Heart
Rat
Rat, 1-2 day
Reinecke H, Zhang M, Bartosek T. and Murry CE.
Survival, integration, and differentiation of cardiomyocyte grafts: a study in normal and injured rat hearts ,
Circulation
 100(2) , 193 , 1999
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 95 u/ml
Pancreatin : 0.06%
Medium
DMEM
Heart
Rat
Rat, Wistar, male
Sambandam N, Abrahani MA, St Pierre E, Al-Atar O, Cam MC. and Rodrigues B.
Localization of lipoprotein lipase in the diabetic heart: regulation by acute changes in insulin ,
Arterioscler Thromb Vasc Biol
 19(6) , 1526 , 1999
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 228 u/ml
Medium
Joklik MEM
Heart
Rat
Rat, adult and neonatal
Richards SM, Jaconi ME, Vassort G. and Puceat M.
A spliced variant of AE1 gene encodes a truncated form of Band 3 in heart: the predominant anion exchanger in ventricular myocytes ,
J Cell Sci
 112 , 1519 , 1999
Cells
Cardioomyocytes
Enzymes
Collagenase Type 4 : 0.12%-adult
Collagenase Type 2 : 0.05%-neonatal
Medium
DMEM/medium 199
Heart
Rat
Rat, SD, 300-350 g
Yu L, Netticadan T, Xu YJ, Panagia V, Dhalla NS.
Mechanisms of lysophosphatidylcholine-induced increase in intracellular calcium in rat cardiomyocytes ,
J Pharmacol Exp Ther
 286 , 1-8 , 1998
Cells
Cardiomyocytes
Enzymes
Medium
see reference
Heart
Rat
Rat, SD, female, 200g
Westfall M V, Rust E M, Metzger J M.
Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function ,
Proc Natl Acad Sci U S A
 94 , 5444-9 , 1997
Cells
Ventricular cardiac myocytes
Enzymes
Medium
Krebs-Henseleit buffer
Heart
Rat
Rat, Wistar, 1 day
Kinugawa, K. , Kohmoto, O. , Yao, A. , Serizawa, T. and Takahashki, T.
Cardiac inducible nitric oxide synthase negatively modulates myocardial function in cultured rat myocytes ,
Am J Physiol
 272 , H35-47 , 1997
Cells
Cardiomyoctes
Enzymes
Collagenase Type 2 : 80 u/ml
Medium
HBSS
Heart
Rat
Rat, SD, fetal and neonatal
Li RK, Mickle DA, Weisel RD, Zhang J, Mohabeer MK.
In vivo survival and function of transplanted rat cardiomyocytes ,
Circ Res
 78 , 283-8 , 1996
Cells
Cardiomyocytes
Enzymes
Collagenase : 0.1%
Trypsin : 0.2%
Medium
PBS
Heart
Rat
Rat, SD, 250-300 g
Sharma VK, Colecraft HM, Wang DX, Levey AI, Grigorenko EV, Yeh HH. and Sheu SS
Molecular and functional identification of m1 muscarinic acetylcholine receptors in rat ventricular myocytes ,
Circ Res
 79(1) , 86 , 1996
Cells
Cardiomyocytes
Enzymes
Medium
Joklik's
Heart
Rat
Rat, SD, male, 200-250 g
Laughlin, M. , Schaeffer, M. and Sturek, M.
Effect of Exercise Training on Intracellular Free Ca2+ Transients in Ventricular Myocytes of Rats ,
Am J Physiol
 73 , 1141 , 1992
Cells
Myocytes
Ventricles
Enzymes
Collagenase : 0.07%
Medium
Joklik's MEM
Heart
Rat
Rat, SD, adult, female, 180-220 g
Fischer, Y. , Rose, H. and Kammermeier. H.
Highly Insulin-Responsive Isolated Rat Heart Muscle Cells Yielded By a Modified Isolation Method ,
Life Sci
 49 , 1679 , 1991
Cells
Cardiomyocytes
Enzymes
Collagenase : 0.11%
Medium
(see reference)
Heart
Rat
Rat, SD, female, 60-90 days
Welder, A.A. , Grant, R. , Bradlaw, J. and Acosta, D.
A Primary Culture System of Adult Rat Heart Cells for the Study of Toxicologic Agents ,
In Vitro Cell Dev Biol
 27 , 921 , 1991
Cells
Myocardial
Enzymes
Medium
Joklik's MEM, Kreb's-Henseleit buffer, CF
Heart
Rat
Rat, neonatal, 1-4 days
Marino, T.A. , Walter, R.,Cobb, E. , Palasiuk, M. , Parsons, T. and Mercer, W.E.
Effects of Norepinephrine on Neonatal Rat Cardiocyte Growth and Differentiation ,
In Vitro Cell Dev Biol
 26 , 229 , 1990
Cells
Cardiomyocytes
Enzymes
Medium
Krebs-Henseleit Buffer, CF
Heart
Rat
Rat, adult, female
Nag, A.C. , Lee, M. and Kosiur, J.R.
Adult Cardiac Muscle Cells in Long-Term Serum-Free Culture:Myofibrillar Organization and Expression of Myosin Heavy Chain Isoforms ,
In Vitro Cell Dev Biol
 26 , 464 , 1990
Cells
Myocytes
Enzymes
Medium
Joklik's MEM
Heart
Rat
Rats, AW, late-gestation pregnant females
Engelmann, G. , McTiernan, C. , Gerrity, R. and Samarel, A.
Serum-free Primary Cultures of Neonatal Rat Cardiomyocytes: Cellular and Molecular Applications ,
Technique
 2 (6) , 279 , 1990
Cells
Ventricular cardiomyocytes
Enzymes
Collagenase Type 2 : 0.05 - 0.08%
Medium
See reference
Heart
Rat
Rat, SD, adult, female, 175 - 225 g
Bugaisky, L.B. and Zak, R.
Differentiation of Adult Rat Cardiac Myocytes in Cell Culture ,
Circ Res
 64 , 493 , 1989
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Medium
Joklik's medium
Heart
Rat
Rat,SD, albino, 10-14 weeks
Berg, I. , Guse, A.H. and Gercken, G.
Carbamoylcholine-Induced Accumulation of Inositol Mono-,Bis-,Tris-, And Tetrakisphosphates In Isolated Cardiac Myocytes From Adult Rats ,
Biochim Biophys Acta
 1010 , 100 , 1989
Cells
Myocytes
Enzymes
Collagenase Type 1 : 100 u/ml and 150 u/ml
Medium
Krebs Ringer bicarbonate buffer
Heart
Rat
Rat, SD, male, 250 - 350 g
De Young, M. , Giannattasio, B. and Scarpa, A.
Isolation of Calcium-Tolerant Atrial And Ventricular Myocytes From Adult Rat Heart , Methods Enzymology Vol. 173 , 662 , 1989
Cells
Myocytes, ventricular
Enzymes
Collagenase Type 1 : 90 and 100 u/ml
Medium
Joklik's solution with and without calcium
Heart
Rat
Rat, adult, 150-200 g
McMahon, K.K.
A Study of Adult Rat Atrial Myocyte Attachment to Extricellular Matrix Components and Long Term Culture , Biology of Isolated Adult Cardiac Myocytes , (Clark, Decker, Borg, Eds) , Elsevier Science Publishing Co. Inc. , 318 , 1988
Cells
Myocytes, atria
Enzymes
Collagenase Type 1 : 100 u/ml
Medium
Kreb's Ringer bicarbonate - HEPES buffer
Heart
Rat
Rat, SD
Buxton, I.L.O. and Doggwiler, K.O.
Alpha1-Adrenergic Receptor Signal Transduction in the Adult Rat Cardiac Myocyte , Biology of Isolated Adult Cardiac Myocytes , (Clark, Decker, Borg, eds) , 298 , 1988
Cells
Myocytes
Enzymes
Medium
Kreb's Ringer w/ calcium
Heart
Rat
Rat, SD, female, 12 weeks
Piper, H.M. , Probst, I. , Schwartz, P. , Hutter, F.J. and Spieckermann, P.G.
Culturing of Calcium Stable Adult Cardiac Myocytes ,
J Mol Cell Cardiol
 14 , 397 , 1982
Cells
Myocytes
Enzymes
Medium
Krebs Ringer bicarbonate buffer
Heart
Rat
Rat, SD, female, 12 wks, 250 g
Powell, T. , Terrar, D.A. and Twist, V.W.
Electrical Properties of Individual Cells Isolated From Adult Rat Ventricular Myocardium ,
J Physiol
 302 , 131 , 1980
Cells
Myocytes
Ventricular myocardium
Enzymes
Medium
Kreb's Ringer bicarbonate buffer
Heart
Rat
Rat, female, adult, 200 - 300 g
Claycomb, W.C. and Palazzo, M.C.
Culture of The Terminally Differentiated Adult Cardiac Muscle Cell: A Light and Scanning Electron Microscope Study ,
Dev Biol
 80 , 466 , 1980
Cells
Myocytes
Enzymes
Medium
Joklik's MEM
Heart
Rat
Rat, 10 wk
Bishop, S. , Oparil, S. , Reynolds, R. and Drummond, J.
Regional Myocyte Size in Normotensive and Spontaneously Hypertensive Rats ,
Hypertension
 1 (4) , 378 , 1979
Cells
Myocytes
Enzymes
Medium
Saline
Heart
Rat
Rat, adult, male, 150-200g
Grosso, D. , Frangakis, C. , Carlson, E. and Bressler, R.
Isolation and Characterization of Myocytes From the Adult Rat Heart ,
Prep Biochem
 7 (5) , 383 , 1977
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Medium
MEM CF
Heart
Rat
Rat, adult
Moustafa, E. , Skomedal, T. , Osnes, J. and Oye, I.
Cyclic Amp Formation and Morphology of Myocardial Cells Isolated from Adult Heart: Effect of Ca2+ and Mg2+ ,
Biochim Biophys Acta
 421 , 411 , 1976
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Medium
Perfusing solution
Heart
Rat
Rat, SD, albino, male, 180-230 g
Glick, M. , Burns, A. and Reddy, W.
Dispersion and Isolation of Beating Cells from Adult Rat Heart ,
Anal Biochem
 61 , 32 , 1974
Cells
Heart ventricles, beating
Enzymes
Collagenase : 0.05-0.2%
Medium
Phosphate buffer
Intestine
Rat
Rat, postnatal, adult
Kruger, G. , Mosher, J. , Bixby, S. , Joseph, N. , Iwashita, T. and Morrison, S.
Neural Crest Stem Cells Persist in the Adult Gut but Undergo Changes in Self-Renewal, Neuronal Subtype Potential, and Factor Responsiveness. ,
Neuron
 35 , 657 , 2002
Cells
Gut neural crest stem
Enzymes
Trypsin : 0.025%
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.