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Tissue References Search Tool

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Eye
Bovine
Bovine
Terlizzi, V. , Kolibabka, M. , Burgess, J. , Hammes, H. and Harmsen, M.
The Pericytic Phenotype of Adipose Tissue-Derived Stromal Cells Is Promoted by NOTCH2. ,
Stem Cells
 36 , 240-251 , 2018
Cells
Retinal endothelial
Enzymes
Pronase : 0.02%
Medium
DMEM
Eye
Bovine
Bovine
Bryan, B. and D'Amore, P.
Pericyte Isolation and USe is Endothelial/Pericyte Coculture Models ,
Meth Enzymol
 443 , 315 , 2008
Cells
Pericyte
Enzymes
Collagenase : 0.2%
Medium
DMEM
Eye
Bovine
Bovine
Bowman, P. , Betz, A. and Goldstein, G.
Primary Culture of Microvascular Endothelial Cells From Bovine Retina ,
In Vitro
 18 (7) , 626 , 1982
Cells
Microvascular endothelial
Enzymes
Medium
MEM
Eye
Chicken
Chick embryo
Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H.
ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent ,
Inv Ophthal Visual Sci
 43 , 3181-9 , 2002
Cells
Corneal epithelia
Enzymes
Collagenase : 0.08%
Trypsin : 0.08%
Medium
HBSS
Eye
Human
Human
Yusoff,B, Riau, A. , Yam, G. , Halim, N. and Mehta, J.
Isolation and Propagation of Human Corneal Stromal Keratocytes for Tissue Engineering and Cell Therapy. ,
Cells
 11 , 178 , 2022
Cells
Corneal stromal keratocytes
Enzymes
Medium
DMEM/F-12
Eye
Human
Human
Sidney, L. , Branch, M. , Dua, H. and Hopkinson, A.
Effect of Culture Medium on Propagation and Phenotype of Corneal Stroma-Derived Stem Cells. ,
Cytotherapy
 17 , 1706-22 , 2015
Cells
Corneal stroma stem
Enzymes
Medium
DMEM
Eye
Human
Human
Du, Y. , Roh, D. , Funderburgh, M. , Mann, M. , Marra, K. , Rubin, J. , Li, X. and Funderburgh, J.
Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro. ,
Mol Vis
 16 , 2680 , 2010
Cells
Corneal stromal stem
Enzymes
Neutral Protease : 1.2 u/ml
Collagenase : 0.1%
Medium
DMEM
Eye
Human
Human, 18-68 yr
Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G.
A novel method of isolation, preservation, and expansion of human corneal endothelial cells ,
Inv Ophthal Visual Sci
 48 , 614-20 , 2007
Cells
Corneal endothelial
Enzymes
Medium
DMEM/F12
Eye
Monkey
Monkey, cynomolgus, young adult
Whittum-Hudson JA, Taylor HR.
Antichlamydial specificity of conjunctival lymphocytes during experimental ocular infection ,
Infect Immun
 57 , 2977 , 1989
Cells
Conjunctival lymphocytes
Enzymes
Medium
RPMI 1640
Eye
Mouse
Mouse
Iqbal, N. , Xu, L. , Devitt, C. and Skapek, S.
Isolation and Characterization of Mammalian Cells Expressing the Arf Promoter During Eye Development. ,
Biotechniques
 56 , 239-49 , 2014
Cells
Vitreous cells
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.03%
Medium
DMEM
Eye
Mouse
Mouse
Schmidt, T. and Kofuji, P.
An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells. ,
J Vis Exp
 47 , 2367 , 2011
Cells
Retinal ganglion
Enzymes
CLSPA : 240 u/ml
Hyaluronidase : 0.2%
Medium
Ames
Eye
Mouse
Mouse, 4-5 week
Ebihara, L. , Tong, J. , Vertel, B. , White, T. and Chen, T.
Properties of Connexin 46 Hemichannels in Dissociated Lens Fiber Cells. ,
Inv Ophthal Visual Sci
 52 , 882-9 , 2011
Cells
Lens Fiber
Enzymes
Protease : 0.5%
Medium
PBS
Eye
Mouse
Mouse, postnatal day 1
Jiang, C. Klassen, H. , Zhang, X. and Young, M.
Laser Injury Promotes Migration and Integration of Retinal Progenitor Cells into Host Retina. ,
Mol Vis
 16 , 983 , 2010
Cells
Retinal progenitor
Enzymes
Collagenase : 0.1%
Medium
HBSS
Eye
Mouse
Mouse
Amadi-Obi, A. , Yu, C. , Liu, X. , Mahdi, R. , Clarke, G. , Nussenblatt, R, Gery, I. , Lee, Y. and Egwuagu, C.
TH17 Cells Contribute to Uveitis and Scleritis and are Expanded by IL-2 and Inhibited by IL-27/STAT1. ,
Nat Med
 13 , 711 , 2007
Cells
Retinal
Enzymes
Medium
RPMI
Eye
Mouse
Mouse, adult
Yoshida, S. , Shimmura, S. , Nagoshi, N. , Fukuda, K. , Matsuzaki, Y. , Okano, H. and Tsubota, K.
Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea. ,
Stem Cells
 24 , 2714-22 , 2006
Cells
Stromal
Enzymes
Trypsin : 0.05%
Collagenase : 78 u/ml
Hyaluronidase : 38 u/ml
Medium
DMEM/F-12
Eye
Mouse
Mouse, 4 week
Su X, Sorenson CM. and Sheibani N.
Isolation and characterization of murine retinal endothelial cells ,
Mol Vis
 9 , 171 , 2003
Cells
Retinal endothelial cells
Enzymes
Medium
DMEM
Eye
Porcine
Porcine, 5-60 kg
Wiencke, A. , Kiilgaard, J. , Nicolini, J. , Bundgaard, M. , Ropke, C. and La Cour, M.
Growth of Cultured Porcine Retinal Pigment Epithelial Cells ,
Acta Opthalmol Scand
 81(2) , 170 , 2003
Cells
Retinal pigment epithelial cells
Enzymes
Medium
DMEM
Eye
Rabbit
Rabbit, New Zealand, Adult
Lai, J. , Cheng, H. and Ma, D.
Investigation of Overrun-Processed Porous Hyaluronic Acid Carriers in Corneal Endothelial Tissue Engineering ,
PLoS ONE
 10 , 1371 , 2015
Cells
Corneal endothelial
Enzymes
Medium
HBSS
Eye
Rabbit
Rabbit
Hao, M. , Flynn, K. , Nien-Shy, C. , Jester, B. , Winkler, M. , Brown, D. , La Schiazza, O. , Bille, J. and Jester, J.
In Vivo Non Linear Optical (NLO) Imaging in Live Rabbit Eyes Using the Heidelbert Two-Photon Laser Opthalmoscope ,
Exp Eye Res
 91 , 308 , 2010
Cells
Corneal keratocytes
Enzymes
Collagenase : 0.2%
Hyaluronidase : 0.05%
Medium
DMEM
Eye
Rabbit
Rabbit, New Zealand
Stramer Brian M, Kwok Michael G K, Farthing-Nayak Pamela J, Jung Jae-Chang, Fini M Elizabeth, Nayak Ramesh C.
Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes ,
Inv Ophthal Visual Sci
 45 , 807-12 , 2004
Cells
Corneal keratocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.5%
Medium
PBS
Eye
Rat
Rat, 4-14 week
Wang N, Koutz CA. and Anderson RE.
A method for the isolation of retinal pigment epithelial cells from adult rats ,
Inv Ophthal Visual Sci
 34(1) , 101 , 1993
Cells
Retinal pigment epithelial cells
Enzymes
Collagenase Type 1 : 65 u/ml
Hyaluronidase : 220 u/ml
Medium
CF Hanks with EDTA
Skin
Canine
Canine
Zheng, Y. , Nace, A. , Chen, W. , Watkins, K. , Sergott, L. , Homan, Y. , Vandeberg, J. , Breen, M. and Stenn, K.
Mature Hair Follicles Generated from Dissociated Cells: A Universal Mechanism of Folliculoneogenesis. ,
Dev Dyn
 239 , 2619-26 , 2010
Cells
Epidermal
Enzymes
Collagenase : 0.35%
Medium
DMEM
Skin
Goat
Goat
Deng, Y. , Hu, J. and Athanasiou, K.
Isolation and Chondroinduction of a Dermis-Isolated, Aggrecan-Sensitive Subpopulation with High Chondrogenic Potential. ,
Arthritis Rheum
 56 , 168 , 2007
Cells
Dermis isolated aggrecan sensitive
Enzymes
Medium
DMEM
Skin
Human

Human

Burja, B. , Paul, D. , Tastanova, A. , Edalat, S. , Gerber, R. , Houtman, M. , Elhai, M. , Bürki, K. , Staeger, R. , Restivo, G. , Lang, R. , Sodin-Semrl, S. , Lakota, K. , Tomšič, M. , Levesque, M. , Distler, O. , Rotar, �. , Robinson, M. and Frank-Bertoncelj, M.
An Optimized Tissue Dissociation Protocol for Single-Cell RNA Sequencing Analysis of Fresh and Cultured Human Skin Biopsies. , Front Cell Dev Biol Vol. 10 , 872688 , 2022
Cells
Skin
Enzymes
Neutral Protease : 2.4 u/ml
Collagenase Type 4 : 1,000 u/ml
Medium
RPMI
Skin
Human
Human
Tan, J. , Jaafar, F. and Makpol, S.
Proteomic Profiling of Senescent Human Diploid Fibroblasts Treated with gamma-tocotrienol. ,
BMC Complement Altern Med
 18 , 314 , 2018
Cells
Diploid fibroblasts
Enzymes
Medium
DMEM
Skin
Human
Human
Botting, Ra. , Bertram, K. , Baharlou, H. , Sandgren, K. , Fletcher, J. , Rhodes, J. , Rana, H. , Plasto, T. , Wang, X. , Lim, J. , Barnouti, L. , Kohout, M. , Papadopoulos, T. , Merten, S. , Olbourne, N. and Cunningham, A.
Phenotypic and Functional Consequences of Different Isolation Protocols on Skin Mononuclear Phagocytes. ,
J Leukoc Biol
 101 , 1393-1403 , 2017
Cells
Mononuclear phagocytes
Enzymes
Neutral Protease : 1 u/ml
Collagenase : 200 u/ml
Trypsin : 0.5%
Medium
RPMI-1640
Skin
Human
Human
Chen, Z. , Wang, X. , Jin, T. , Wang, Y. , Hong, C. , Tan, L. , Dai, T. , Wu, L. , Zhuang, Z. and Shi, C.
Increase in the Radioresistance of Normal Skin Fibroblasts but not Tumor Cells by Mechanical Injury. ,
Cell Death Dis
 8 , e2573 , 2017
Cells
Fibroblasts
Enzymes
Medium
DMEM
Skin
Human
Human
Douvaras, P. , Wang, J. , Zimmer, M. , Hanchuk, S. , O'Bara, M. , Sadiq, S. , Sim, F. , Goldman, J. and Fossati, V.
Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells. ,
Stem Cell Reports
 3 , 250 , 2014
Cells
Skin fibroblasts
Enzymes
Collagenase Type 1 : 1000 u/ml
Medium
DMEM
Skin
Human
Human
Cheuk, S. , Wiken, M. , Blomqvist, L. , Nylen, S. , Talme, T. and Stahle, M.and Eidsmo, L.
Epidermal Th22 and Tc17 Cells Form a Localized Disease Memory in Clinically Healed Psoriasis. ,
J Immunol
 192 , 3111 , 2014
Cells
Skin
Enzymes
Medium
RPMI 1640
Skin
Human
Human
Cerny, D. , Haniffa, M. , Shin, A. , Bigliardi, P. , Tan, B. , Lee, B. , Poidinger, M. , Tan, E. , Ginhoux, F. and Fink, K.
Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. ,
PLoS Pathog
 10 , e1004548 , 2014
Cells
Skin
Enzymes
Medium
RPMI
Skin
Human
Human
Carriel, V. , Garzon, I. , Jimenez, J. , Oliveira, A. , Arias-Santiago, S. , Campos, A. , Sanchez-Quevedo, M. and Alaminos, M.
Epithelial and Stromal Developmental Patterns in a Novel Substitute of the Human Skin Generated with Fibrin-Agarose Biomaterials. ,
Cells Tissues Organs
 196 , 1-12 , 2012
Cells
Fibroblasts and keratinocytes
Enzymes
Medium
DMEM
Skin
Human
Human
Karumbayaram, S. , Lee, P. , Azghadi, S. , Cooper, A. , Patterson, M. , Kohn, D. , Pyle, A. , Clark, A. , Byrne, J. , Zack, J. , Plath, K. and Lowry, W.
From Skin Biopsy to Neurons Through a Pluripotent Intermediate Under Good Manufacturing Practice Protocols ,
Stem Cells Transl Med
 1 , 36 , 2012
Cells
Fibroblasts
Enzymes
Medium
HBSS
Skin
Human
Human
Zheng, Y. , Nace, A. , Chen, W. , Watkins, K. , Sergott, L. , Homan, Y. , Vandeberg, J. , Breen, M. and Stenn, K.
Mature Hair Follicles Generated from Dissociated Cells: A Universal Mechanism of Folliculoneogenesis. ,
Dev Dyn
 239 , 2619-26 , 2010
Cells
Epidermal
Enzymes
Collagenase : 0.35%
Medium
DMEM
Skin
Human
Human
Zhang, Q. , Yamaza, T. , Kelly, AP, Shi, S. , Wang, S. , Brown, J. , Wang, L. , French, S. , Shi, S. and Le, A.
Tumor-like Stem Cells Derived from Human Keloid are Governed by the Inflammatory Niche Driven by IL-17/IL-6 Axis. ,
PLoS ONE
 4 , e7798 , 2009
Cells
Skin and keloid progenitor
Enzymes
Medium
PBS
Skin
Human
Human
Tuan, T. , Hwu, P. , Ho, W. , Yiu, P. , Chang, R. , Wysocki, A. and Benya, P.
Adenoviral Overexpression and Small Interfering RNA Suppression Demonstrate that Plasminogen Activator Inhibitor-1 Produces Elevated Collagen Accumulation in Normal and Keloid Fibroblasts. ,
Am J Pathol
 173 , 1311-25 , 2008
Cells
Fibroblasts
Enzymes
Collagenase Type 1 : 100-320 u/ml
Medium
DMEM
Skin
Human
Human, 6-12 year
Chen, F. , Zhang, W. , Bi, D. , Liu, W. , Wei, X. , Chen, F. , Zhu, L. , Cui, L. and Cao, Y.
Clonal Analysis of Nestin(-) Vimentin(+) Multipotent Fibroblasts Isolated from Human Dermis. ,
J Cell Sci
 120 , 2875 , 2007
Cells
Dermal fibroblasts
Enzymes
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.