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Tissue References Search Tool

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Eye
Bovine
Bovine
Terlizzi, V. , Kolibabka, M. , Burgess, J. , Hammes, H. and Harmsen, M.
The Pericytic Phenotype of Adipose Tissue-Derived Stromal Cells Is Promoted by NOTCH2. ,
Stem Cells
 36 , 240-251 , 2018
Cells
Retinal endothelial
Enzymes
Pronase : 0.02%
Medium
DMEM
Eye
Bovine
Bovine
Bryan, B. and D'Amore, P.
Pericyte Isolation and USe is Endothelial/Pericyte Coculture Models ,
Meth Enzymol
 443 , 315 , 2008
Cells
Pericyte
Enzymes
Collagenase : 0.2%
Medium
DMEM
Eye
Bovine
Bovine
Bowman, P. , Betz, A. and Goldstein, G.
Primary Culture of Microvascular Endothelial Cells From Bovine Retina ,
In Vitro
 18 (7) , 626 , 1982
Cells
Microvascular endothelial
Enzymes
Medium
MEM
Eye
Chicken
Chick embryo
Reenstra Wende R, Orlow Daniel L, Svoboda Kathy K H.
ECM-stimulated signaling and actin reorganization in embryonic corneal epithelia are Rho dependent ,
Inv Ophthal Visual Sci
 43 , 3181-9 , 2002
Cells
Corneal epithelia
Enzymes
Collagenase : 0.08%
Trypsin : 0.08%
Medium
HBSS
Eye
Human
Human
Yusoff,B, Riau, A. , Yam, G. , Halim, N. and Mehta, J.
Isolation and Propagation of Human Corneal Stromal Keratocytes for Tissue Engineering and Cell Therapy. ,
Cells
 11 , 178 , 2022
Cells
Corneal stromal keratocytes
Enzymes
Medium
DMEM/F-12
Eye
Human
Human
Sidney, L. , Branch, M. , Dua, H. and Hopkinson, A.
Effect of Culture Medium on Propagation and Phenotype of Corneal Stroma-Derived Stem Cells. ,
Cytotherapy
 17 , 1706-22 , 2015
Cells
Corneal stroma stem
Enzymes
Medium
DMEM
Eye
Human
Human
Du, Y. , Roh, D. , Funderburgh, M. , Mann, M. , Marra, K. , Rubin, J. , Li, X. and Funderburgh, J.
Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro. ,
Mol Vis
 16 , 2680 , 2010
Cells
Corneal stromal stem
Enzymes
Neutral Protease : 1.2 u/ml
Collagenase : 0.1%
Medium
DMEM
Eye
Human
Human, 18-68 yr
Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G.
A novel method of isolation, preservation, and expansion of human corneal endothelial cells ,
Inv Ophthal Visual Sci
 48 , 614-20 , 2007
Cells
Corneal endothelial
Enzymes
Medium
DMEM/F12
Eye
Monkey
Monkey, cynomolgus, young adult
Whittum-Hudson JA, Taylor HR.
Antichlamydial specificity of conjunctival lymphocytes during experimental ocular infection ,
Infect Immun
 57 , 2977 , 1989
Cells
Conjunctival lymphocytes
Enzymes
Medium
RPMI 1640
Eye
Mouse
Mouse
Iqbal, N. , Xu, L. , Devitt, C. and Skapek, S.
Isolation and Characterization of Mammalian Cells Expressing the Arf Promoter During Eye Development. ,
Biotechniques
 56 , 239-49 , 2014
Cells
Vitreous cells
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.03%
Medium
DMEM
Eye
Mouse
Mouse
Schmidt, T. and Kofuji, P.
An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells. ,
J Vis Exp
 47 , 2367 , 2011
Cells
Retinal ganglion
Enzymes
CLSPA : 240 u/ml
Hyaluronidase : 0.2%
Medium
Ames
Eye
Mouse
Mouse, 4-5 week
Ebihara, L. , Tong, J. , Vertel, B. , White, T. and Chen, T.
Properties of Connexin 46 Hemichannels in Dissociated Lens Fiber Cells. ,
Inv Ophthal Visual Sci
 52 , 882-9 , 2011
Cells
Lens Fiber
Enzymes
Protease : 0.5%
Medium
PBS
Eye
Mouse
Mouse, postnatal day 1
Jiang, C. Klassen, H. , Zhang, X. and Young, M.
Laser Injury Promotes Migration and Integration of Retinal Progenitor Cells into Host Retina. ,
Mol Vis
 16 , 983 , 2010
Cells
Retinal progenitor
Enzymes
Collagenase : 0.1%
Medium
HBSS
Eye
Mouse
Mouse
Amadi-Obi, A. , Yu, C. , Liu, X. , Mahdi, R. , Clarke, G. , Nussenblatt, R, Gery, I. , Lee, Y. and Egwuagu, C.
TH17 Cells Contribute to Uveitis and Scleritis and are Expanded by IL-2 and Inhibited by IL-27/STAT1. ,
Nat Med
 13 , 711 , 2007
Cells
Retinal
Enzymes
Medium
RPMI
Eye
Mouse
Mouse, adult
Yoshida, S. , Shimmura, S. , Nagoshi, N. , Fukuda, K. , Matsuzaki, Y. , Okano, H. and Tsubota, K.
Isolation of Multipotent Neural Crest-Derived Stem Cells from the Adult Mouse Cornea. ,
Stem Cells
 24 , 2714-22 , 2006
Cells
Stromal
Enzymes
Trypsin : 0.05%
Collagenase : 78 u/ml
Hyaluronidase : 38 u/ml
Medium
DMEM/F-12
Eye
Mouse
Mouse, 4 week
Su X, Sorenson CM. and Sheibani N.
Isolation and characterization of murine retinal endothelial cells ,
Mol Vis
 9 , 171 , 2003
Cells
Retinal endothelial cells
Enzymes
Medium
DMEM
Eye
Porcine
Porcine, 5-60 kg
Wiencke, A. , Kiilgaard, J. , Nicolini, J. , Bundgaard, M. , Ropke, C. and La Cour, M.
Growth of Cultured Porcine Retinal Pigment Epithelial Cells ,
Acta Opthalmol Scand
 81(2) , 170 , 2003
Cells
Retinal pigment epithelial cells
Enzymes
Medium
DMEM
Eye
Rabbit
Rabbit, New Zealand, Adult
Lai, J. , Cheng, H. and Ma, D.
Investigation of Overrun-Processed Porous Hyaluronic Acid Carriers in Corneal Endothelial Tissue Engineering ,
PLoS ONE
 10 , 1371 , 2015
Cells
Corneal endothelial
Enzymes
Medium
HBSS
Eye
Rabbit
Rabbit
Hao, M. , Flynn, K. , Nien-Shy, C. , Jester, B. , Winkler, M. , Brown, D. , La Schiazza, O. , Bille, J. and Jester, J.
In Vivo Non Linear Optical (NLO) Imaging in Live Rabbit Eyes Using the Heidelbert Two-Photon Laser Opthalmoscope ,
Exp Eye Res
 91 , 308 , 2010
Cells
Corneal keratocytes
Enzymes
Collagenase : 0.2%
Hyaluronidase : 0.05%
Medium
DMEM
Eye
Rabbit
Rabbit, New Zealand
Stramer Brian M, Kwok Michael G K, Farthing-Nayak Pamela J, Jung Jae-Chang, Fini M Elizabeth, Nayak Ramesh C.
Monoclonal antibody (3G5)-defined ganglioside: cell surface marker of corneal keratocytes ,
Inv Ophthal Visual Sci
 45 , 807-12 , 2004
Cells
Corneal keratocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.5%
Medium
PBS
Eye
Rat
Rat, 4-14 week
Wang N, Koutz CA. and Anderson RE.
A method for the isolation of retinal pigment epithelial cells from adult rats ,
Inv Ophthal Visual Sci
 34(1) , 101 , 1993
Cells
Retinal pigment epithelial cells
Enzymes
Collagenase Type 1 : 65 u/ml
Hyaluronidase : 220 u/ml
Medium
CF Hanks with EDTA
Miscellaneous
Bovine
Bovine
Lee, J. , Cheung, K. and Leung, V.
Systematic Study of Cell Isolation from Bovine Nucleus Pulposus: Improving Cell Yield and Experiment Reliability. ,
J Orthop Res
 33 , 1743-55 , 2015
Cells
Nucleus pulposus
Enzymes
Medium
DMEM
Miscellaneous
Canine

Canine, Rat, Rabbit, Ovine, Bovine, Human

Basatvat, S. , Bach, F. , Barcellona, M. , Binch, A. , Buckley, C. , Bueno, B. , Chahine, N. , Chee, A. and Maitre, C.
Harmonization and Standardization of Nucleus Pulposus Cell Extraction and Culture Methods. ,
Spine
 6 , e1238 , 2023
Cells
nucleus pulposus
Enzymes
Pronase : 0.075%
Medium
DMEM
Miscellaneous
Equine
Equine
Nemoto, M. , Kizaki, K. , Yamamoto, Y. , Oonuma, T. and Hashizume, K.
Tenascin-C Expression in Equine Tendon-derived Cells During Proliferation and Migration. ,
J Equine Sci
 24 , 17-24 , 2013
Cells
Tendon Cells
Enzymes
Medium
DMEM
Miscellaneous
Goat
Goat
Arora, A. , Sriram, M. , Kothari, A. and Katti, D.
Co-culture of Infrapatellar Fat Pad-Derived Mesenchymal Stromal Cells and Articular Chondrocytes in Plasma Clot for Cartilage Tissue Engineering. ,
Cytotherapy
 19 , 881-894 , 2017
Cells
Infrapatellar fat pad mesenchymal stromal
Enzymes
Collagenase : 0.15%
Medium
DMEM
Miscellaneous
Human

Human, 18-30 years

Pardo-Rodríguez, B. , Baraibar, A. , Manero-Roig, I. , Luzuriaga, J. , Salvador-Moya, J. , Polo, Y. , Basanta-Torres, R. , Unda, F. , Mato, S. , Ibarretxe, G. and Pineda, J.
Functional Differentiation of Human Dental Pulp Stem Cells into Neuron-like Cells Exhibiting Electrophysiological Activity. ,
Stem Cell Res Ther
 16 , 10 , 2025
Cells
Dental pulp stem cells
Enzymes
Medium
DMEM
Miscellaneous
Human
Human
Foster, D. , Marshall, C. , Gulati, G. , Chinta, M. , Nguyen, A. , Salhotra, A. , Jones, R. , Burcham, A. , Lerbs, T. , Cui, L. , King, M. , Titan, A. , Ransom, R. , Manjunath, A. , Hu, M. and Blackshear, C.
Elucidating the Fundamental Fibrotic Processes Driving Abdominal Adhesion Formation. ,
Nat Commun
 11 , 4061 , 2020
Cells
Adhesion fibroblasts
Enzymes
Medium
Medium 199
Miscellaneous
Human
Human
Sun, Y. , Lv, M. , Zhou, L. , Tam, V. , Lv, F. , Chan, D. , Wang, H. , Zheng, Z. , Cheung, K. and Leung, V.
Enrichment of Committed Human Nucleus Pulposus Cells Expressing Chondroitin Sulfate Proteoglycans under Alginate Encapsulation. ,
Osteoarthritis Cartilage
 23 , 1194-203 , 2015
Cells
Nucleus pulposus
Enzymes
Pronase : 0.25%
Collagenase Type 2 : 600 u/ml
Medium
DMEM
Miscellaneous
Human
Human, 3-7 year
Salmon, B. , Bardet, C, Khaddam, M. Naji, J. , Coyac, B. , Baroukh, B. , Letourneur, F. , Lesieur, J. , Decup, F. , Le Denmat, D. , Nicoletti, A. , Poliard, A, Rowe, P. , Huet, E. , Vital, S. and Linglart, A.
MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia. ,
PLoS ONE
 8 , e56749 , 2013
Cells
Dental pulp stem cells
Enzymes
Medium
DMEM
Miscellaneous
Human
Human
Bonnamain, V. , Thinard, R. , Sergent-Tanguy, S. , Huet, P. , Bienvenu, G. , Naveilhan, P. , Farges, J. and Alliot-Licht, B.
Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium. ,
Front Physiol
 4 , 357 , 2013
Cells
Dental pulp stem
Enzymes
Medium
DMEM/F12
Miscellaneous
Human
Human
Lee, Y. , Lee, J. , Park, H. , Lim, Y. , Lee, J. , Wang, S. and Lee, B.
Isolation of Mesenchymal Stromal Cells (MSCs) from Human Adenoid Tissue. ,
Cell Physiol Biochem
 31 , 513-24 , 2013
Cells
Mesenchymal stromal
Enzymes
Medium
PBS
Miscellaneous
Human
Human
Ozdener, H. , Spielman, A. and Rawson, N.
Isolation and Culture of Human Fungiform Taste Papillae Cells ,
J Vis Exp
 63 , e3730 , 2012
Cells
Fungiform taste papillae
Enzymes
Medium
see reference
Miscellaneous
Human
Human
Pauly, S. , Klatte, F. , Strobel, C. , Schmidmaier, G. , Greiner, S. and Scheibel, M. Wildemann B
Characterization of Tendon Cell Cultures of the Human Rotator Cuff. ,
Eur Cell Mater
 20 , 84-97 , 2010
Cells
Tendon cells
Enzymes
Medium
DMEM/Hams F12
Miscellaneous
Human
Human
Panetta, N. , Gupta, D. , Kwan, M. , Wan, D. , Commons, G. and Longaker, M.
Tissue Harvest by Means of Suction-Assisted or Third-Generation Ultrasound-Assisted Lipoaspiration Has No Effect on Osteogenic Potential of Human Adipose-Derived Stromal Cells. ,
Plast Reconstr Surg
 124 , 65 , 2009
Cells
Adipose derived stromal
Enzymes
Medium
DMEM
Miscellaneous
Human
Human
Patino WillmarD, Kang Ju-Gyeong, Matoba Satoaki, Mian OmarY, Gochuico BernadetteR, Hwang PaulM.
Atherosclerotic plaque macrophage transcriptional regulators are expressed in blood and modulated by tristetraprolin ,
Circ Res
 98 , 1282-9 , 2006
Cells
Carotid artery plaque macrophage
Enzymes
Medium
HBSS
Miscellaneous
Human
Human
Rafiee, P. , Ogawa, H. , Heidemann, J. , Li, M. , Aslam, M. , Lamirand, T. , Fisher, P. , Graewin, S. , Dwinell, M. , Johnson, C. , Shaker, R. and Binion, D.
Isolation and Characterization of Human Esophageal Microvascular Endothelial Cells: Mechanisms of Inflammatory Activation. ,
Am J Physiol Gastrointest Liver Physiol
 285 , G1277 , 2003
Cells
Esophageal microvascular endothelial
Enzymes
Medium
MCDB-131

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.