Tissue References Search Tool

Bovine

Calf, male, less then 6 months old

Hassan, H. and Merkel, R.

Perifusion Model System to Culture Bovine Hypothalamic Slices In Series with Dispersed Anterior Pituitary Cells ,
In Vitro Cell Dev Biol
30A , 435 , 1994

Cells

Pituitary

Enzymes

Collagenase : 0.3%

Medium

DMEM

Pituitary

Bovine

Mason, W. and Ingram, C.

Techniques for Studying the Role of Electrical Activity in Control of Secretion by Normal Anterior Pituitary Cells , Vol. 124 , 207 , 1986

Cells

Pituitary

Enzymes

Collagenase : 0.1%

Medium

EBSS, CMF

Pituitary

Mouse

Perez Millain, M. , Brinkmeier, M. , Mortensen, A. and Camper, S.

PROP1 Triggers Epithelial-Mesenchymal Transition-Like Process in Pituitary Stem Cells. ,
Elife
5 , 2016

Cells

Pituitary

Enzymes

Collagenase Type 2 : 0.5%

Deoxyribonuclease I : 0.005%

Medium

HBSS

Pituitary

Mouse

Pyczek, J. , Buslei, R. , Schult, D. , Holsken, A. , Buchfelder, M. , Hess, I. , Hahn, H. and Uhmann, A.

Hedgehog Signaling Activation Induces Stem Cell Proliferation and Hormone Release in the Adult Pituitary Gland. ,
Sci Rep
6 , 24928 , 2016

Cells

Pituitary

Enzymes

Collagenase Type 1 : 1,000 u/ml

Soybean Trypsin Inhibitor : 0.01%

Hyaluronidase : 0.1%

Deoxyribonuclease I : 0.001%

Medium

DMEM

Pituitary

Mouse

Mouse, male

Steveson Tami C, Ciccotosto Giuseppe D, Ma Xin-Ming, Mueller Gregory P, Mains Richard E, Eipper Betty A.

Menkes protein contributes to the function of peptidylglycine alpha-amidating monooxygenase ,
Endocrinology
144 , 188-200 , 2003

Cells

Pituitary

Enzymes

Collagenase : 0.4%

Hyaluronidase : 0.1%

Trypsin : 0.3%

Medium

DMEM/Han's F12

Pituitary

Ovine

Ovine, adult

Xu Ruwei, Wang Qinling, Yan Ming, Hernandez Maria, Gong Changhong, Boon WahChin, Murata Yoko, Ueta Yoichi, Chen Chen.

Orexin-A augments voltage-gated Ca2+ currents and synergistically increases growth hormone (GH) secretion with GH-releasing hormone in primary cultured ovine somatotropes ,
Endocrinology
143 , 4609-19 , 2002

Cells

Somatotropes

Enzymes

Collagenase Type 1 : 0.3%

Medium

Medium 199

Pituitary

Rat

Rat, male, 12-15 week

Akieda-Asai, S. , Zaima, N. , Ikegami, K. , Kahyo, T, Yao, I. , Hatanaka, T. , Iemura, S. , Sugiyama, R. , Yokozeki, T. , Eishi, Y. , Koike, M. , Ikeda, K. , Chiba, T. , Yamaza, H. , Shimokawa, I. , Song, S. , Matsuno, A. , Mizutani, A. , Sawabe, M. Chao, N. and Tanaka, M.

SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5K gamma Activity through Deacetylation of Specific Lysine Residues in Mammals. ,
PLoS ONE
5 , e11755 , 2010

Cells

Pituitary

Enzymes

Collagenase Type 2 : 0.4%

Deoxyribonuclease I : 0.04%

Medium

DMEM

Thymus

Human

Vandenabeele S, Hochrein H, Mavaddat N, Winkel K, Shortman K.

Human thymus contains 2 distinct dendritic cell populations ,
Blood
97 , 1733-41 , 2001

Cells

Dentritic

Enzymes

Collagenase Type 2 : 0.1%

Deoxyribonuclease I : 0.002%

Medium

RPMI 1640

Thymus

Mouse

Rode, I. and Boehm, T.

Regenerative Capacity of Adult Cortical Thymic Epithelial Cells. ,
Proc Natl Acad Sci U S A
109 , 3463-8 , 2012

Cells

Thymic stromal

Enzymes

Collagenase Type 4 : 0.02%

Neutral Protease : 0.02%

Deoxyribonuclease I : 0.0025%

Medium

RPMI-1640

Thymus

Mouse

Phillips, Joy, Brondstetter, T. , English, C. , Lee, H. , Virts, E. and Thoman, M.

IL-7 Gene Therapy in Aging Restores Early thymopoiesis without Reversing Involution ,
J Immunol
, 4869 , 2004

Cells

Stromal

Enzymes

Collagenase Type 3 : 0.2%

Hyaluronidase : 0.1%

Medium

RPMI 1640

Thymus

Mouse

Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F. and Gabrieli, L

ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells ,
J Exp Med
196 , 1415 , 2002

Cells

Dentritic

Enzymes

Collagenase Type 3 : 0.1%

Deoxyribonuclease I : 325 u/ml

Medium

RPMI 1640

Thymus

Mouse

Mouse, 3-6 week

Smith KM, Olson DC, Hirose R, Hanahan D.

Pancreatic gene expression in rare cells of thymic medulla: evidence for functional contribution to T cell tolerance ,
Int Immunol
9 , 1355-65 , 1997

Cells

Thymic

Enzymes

Collagenase Type 3 : 100-400 u/ml

Medium

HBSS

Thymus

Mouse

Mouse, C3H, 16 wk old, female

Ehmann, U. , Shiurba, R. and Peterson, W.

Long-Term Proliferation of Mouse Thymic Epithelial Cells in Culture ,
In Vitro Cell Dev Biol
22 (12) , 738 , 1986

Cells

Epithelial

Enzymes

Collagenase Type 3 : 0.1%

Medium

DMEM

Thymus

Mouse

Mouse, Swiss, 6 wk

Jones, K. and Pierre, R.

Analysis of Cellular Heterogeneity in Mouse Thymus Cultures ,
In Vitro
17 , 431 , 1981

Cells

Thymus

Enzymes

Collagenase Type 3 : 150 u/ml

Medium

DMEM

Thymus

Rat

Masuda, A. and Matsuyama, M

Epithelial Cell Lines From Rat Thymoma and Rat Thymus , Cell & Tissue Culture: Laboratory Procedures Vol. 2 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 21C:4.1 , 1995

Cells

Thymic epithelial

Enzymes

Collagenase Type 3 : 0.1%

Medium

DMEM

Thymus

Rat

Rat (ACI/NMs X BUF/Mna) F1, male, 28 months
Rat, (ACI/MNs) male, 8 weeks
(also bovine, adult)

Masuda, A. , Ohtsuka, K. and Matsuyama, M.

Establishment of Functional Epithelial Cell Lines from a Rat Thyoma and a Rat Thymus ,
In Vitro Cell Dev Biol
26 , 713 , 1990

Cells