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Tissue References Search Tool

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Reproductive
Human
Human
Yusuf RZ, Naeem R.
Cytogenetic studies of spontaneous miscarriages: a seven year study to compare significance of primary vs. secondary culture methods for assessment of fetal karyotype yield and maternal cell contamination ,
Early Pregnancy
 5 , 121-31 , 2001
Cells
Chorionic villi
Enzymes
Trypsin : see reference
Collagenase Type 3 : 100 u/ml
Medium
HBSS
Reproductive
Human
Human, female, 20-40 year
Gargett CE, Bucak K, Rogers PA.
Isolation, characterization and long-term culture of human myometrial microvascular endothelial cells ,
Hum Reprod
 15 , 293-301 , 2000
Cells
Microvascular endothelial cells
Enzymes
Medium
PBS
Reproductive
Human
Human
Friden BE, Runesson E, Hahlin M, Brannstrom M.
Evidence for nitric oxide acting as a luteolytic factor in the human corpus luteum ,
Mol Hum Reprod
 6 , 397-403 , 2000
Cells
Corpus luteum cells
Enzymes
Medium
PBS
Reproductive
Human
Human
Nikitenko LL, MacKenzie IZ,Rees MC, Bicknell R.
Adrenomedullin is an autocrine regulator of endothelial growth in human endometrium ,
Mol Hum Reprod
 6 , 811 , 2000
Cells
Endometrial endothelial cells
Enzymes
Medium
McCoys medium
Reproductive
Human
Human
Runesson E, Ivarsson K, Janson P, Brannstrom M.
Gonadotropin- and cytokine-regulated expression of the chemokine interleukin 8 in the human preovulatory follicle of the menstrual cycle ,
J Clin Endocrinol Metab
 85 , 4387-95 , 2000
Cells
Theca cells
Enzymes
Medium
PBS
Reproductive
Human
Human, female
Zhang J, Lathbury LJ, Salamonsen LA.
Expression of the chemokine eotaxin and its receptor, CCR3, in human endometrium ,
Biol Reprod
 62 , 404-11 , 2000
Cells
Endometrial epithelial cells
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human, females, 25-45 yr
Friden BE, Hagstrom H, Lindblom B, Sjoblom P, Wallin A, Brannstrom M, Hahlin M.
Cell characteristics and function of two enriched fraction of human luteal cells prolonged culture ,
Mol Hum Reprod
 5 , 714-9 , 1999
Cells
Luteal cells
Enzymes
Medium
PBS
Reproductive
Human
Human, female, 25-41 year
Hovatta O, Wright C, Krausz T, Hardy K, Winston RM.
Human primordial, primary and secondary ovarian follicles in long-term culture: effect of partial isolation ,
Hum Reprod
 14 , 2519-24 , 1999
Cells
Follicles
Enzymes
Collagenase Type 2 : 0.025-0.1%
Medium
EBSS
Reproductive
Human
Human, female
Huang JC, Liu DY, Dawood MY.
The expression of vascular endothelial growth factor isoforms in cultured human endometrial stromal cells and its regulation by 17beta-oestradiol ,
Mol Hum Reprod
 4 , 603-7 , 1998
Cells
Endometrial stromal cells
Enzymes
Collagenase : 4000 u/ml
Medium
DMEM/F-12
Reproductive
Human
Human
Bradbury. , Sunn, K. , Crossley, M. , Bai, M. , Brown, E. , Delbridge, D. and Conigrave, A.
Expression of the Parathyroid Ca 2+ - Sensing Receptor in Cytotrophoblasts From Human Term Placenta ,
J Endocrinol
 156 , 425-430 , 1998
Cells
Placental
Enzymes
Medium
PSS
Reproductive
Human
Human
Zhang J, Nie G, Jian W, Woolley DE, Salamonsen LA.
Mast cell regulation of human endometrial matrix metalloproteinases: A mechanism underlying menstruation ,
Biol Reprod
 59 , 693-703 , 1998
Cells
Stromal endometrial
Enzymes
Medium
DMEM/Ham's F12
Reproductive
Human
Human
Takeuchi, K. , Maruyama, I. , Yamamoto, S. , Oki, T. and Nagata, Y.
Isolation and Monolayer Culture of Human Fallopian Tube Epithelial Cells ,
In Vitro Cell Dev Biol
 27 , 720 , 1991
Cells
Epithelial, fallopian tube
Enzymes
Medium
Medium 199
Reproductive
Human
Human, female
Rinehart, C. , Lyn-Cook, B. and Kaufman, D.
Gland formation from human endometrial epithelial cells in vitro ,
In Vitro Cell Dev Biol
 24 (10) , 1037 , 1988
Cells
Endometrial
Enzymes
Medium
RPMI 1640
Reproductive
Human
Human
Siegfried, J. , Nelson, K. , Martin, J. and Kaufman, D.
Histochemical Identification of Cultured Cells From Human Endometrium ,
In Vitro
 20 (1) , 25 , 1984
Cells
Epithelial
Stromal
Enzymes
Collagenase : 0.25%
Medium
See reference
Reproductive
Human
Human
Kirk, D. , King, R. , Heyes, J. , Peachey, L. , Hirsch, P. and Taylor, W.
Normal Human Edometrium in Cell Culture ,
In Vitro
 14 (8) , 651 , 1978
Cells
Epithelial
Stromal
Enzymes
Collagenase Type 1 : 180 u/ml
Medium
DMEM
Reproductive
Insect
Drosophila
Ma, X. , Wang, S. , Do, T. , Song, X. , Inaba, M. , Nishimoto, Y. , Liu, L. , Gao, Y. , Mao, Y. , Li, H. , McDowell, W. , Park, J. , Malanowski, K. , Peak, A. , Perera, A. and Li, H. et al.
Piwi is Required in Multiple Cell Types to Control Germline Stem Cell Lineage Development in the Drosophila Ovary. ,
PLoS ONE
 9 , e90267 , 2014
Cells
Ovarian
Enzymes
Medium
Grace's
Reproductive
Insect
Drosophila, embryonic
Salmand, P. Iche-Torres, M. and Perrin, L.
Tissue-Specific Cell Sorting from Drosophila Embryos: Application to Gene Expression Analysis. , Fly (Austin) Vol. 5 , 261
Cells
Cardiac differentiating
Enzymes
Collagenase Type 1 : 20 u/ml
Trypsin : 0.25%
Medium
Schneider
Reproductive
Monkey
Rhesus macaque
Xu, J, Lawson, MS, Yeoman, RR, Pau, KY, Barrett, SL, Zelinski, MB. and Stouffer, RL
Secondary Follicle Growth and Oocyte Maturation During Encapsulated Three-Dimensional Culture in Rhesus Monkeys: Effects of Gonadotrophins, Oxygen and Fetuin. ,
Hum Reprod
 26 , 1061-72 , 2011
Cells
Follicle
Enzymes
Medium
HEPES
Reproductive
Mouse
Mouse, 4 week
Hou, M. , Han, J. , Li, G. , Kwon, M. , Jiang, J. , Emani, S. , Taglauer, E. , Park, J. , Choi, E. , Vodnala, M. , Fong, Y. , Emani, S. , Rosas, I. , Perrella, M. and Liu, X.
Multipotency of Mouse Trophoblast Stem Cells ,
Stem Cell Res Ther
 11 , 55 , 2020
Cells
Trophoblast
Enzymes
Medium
DMEM
Reproductive
Mouse
Mouse, adult
Lee, A, Rusch, J. , Lima, A. , Usmani, A. , Huang, N. , Lepamets, M. , Vigh-Conrad, K. , Worthington, R. and et al.
Rare Mutations in the Complement Regulatory Gene CSMD1 are Associated with Male and Female Infertility. ,
Nat Commun
 10 , 4626 , 2019
Cells
Testes
Enzymes
Medium
MEM
Reproductive
Mouse
Mouse, 1-7 day
Kang, H. , Chen, L. , Lichti-Kaiser, K. , Liao, G. , Gerrish, K. , Bortner, C. , Yao, Hu. , Eddy, E. and Jetten, A.
Transcription Factor GLIS3: A New and Critical Regulator of Postnatal Stages of Mouse Spermatogenesis. ,
Stem Cells
 34 , 2772-2783 , 2016
Cells
Testicular
Enzymes
Medium
HBSS
Reproductive
Mouse
Mouse, adult
Martin, L. and Seandel, M.
Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-Term Adult Mouse SSC Lines. ,
J Vis Exp
 72 , e50017 , 2013
Cells
Spermatogonial stem
Enzymes
Trypsin : 0.05%
Medium
DMEM
Reproductive
Mouse
Mouse, female
Jiang, J. and Kelly, K.
Isolation of Lymphocytes from Mouse Genital Tract Mucosa. ,
J Vis Exp
 67 , e4391 , 2012
Cells
Lymphocytes
Enzymes
Collagenase : 450 u/ml
Medium
RPMI
Reproductive
Mouse
Mouse
White, Y. , Woods, D. , Takai, Y. , Ishihara, O. , Seki, H. and Tilly, J.
Oocyte Formation by Mitotically Active Germ Cells Purified from Ovaries of Reproductive-Age Aomen. ,
Nat Med
 18 , 413 , 2012
Cells
Ovarian
Enzymes
Collagenase Type 4 : 800 u/ml
Medium
HBSS
Reproductive
Mouse
Mouse
Venkataraman, L. , Lenis, A. , Couri, B. , Damaser, M. and Ramarurthi, A
Induced Regenerative Elastic Matrix Repair in LOXL 1 Knockout Mouse Cell Cultures: Towards Potential Therapy for Pelvic Organ Prolapse ,
J Tissue Sci Eng
 3 , 3 , 2012
Cells
Vaginal smooth muscle
Enzymes
Collagenase Type 2 : 175 u/ml
Elastase : 0.025%
Medium
DMEM/F12
Reproductive
Mouse
Mouse
Yamatoya, K. , Ito, C. , Araki, M. , Furuse, R. and Toshimori, K.
One-Step Collagenase Method for Zona Pellucida Removal in Unfertilized Eggs: Easy and Gentle Method for Large-Scale Preparation. ,
Reprod Med Bio
 10 , 97-103 , 2011
Cells
Zona pellucid free eggs
Enzymes
Collagenase : 0.1%
Medium
THY-HEPES
Reproductive
Mouse
Mouse, male
Getun, I. , Torres, B. and Bois, P.
Flow Cytometry Purification of Mouse Meiotic Cells. ,
J Vis Exp
 50 , 2602 , 2011
Cells
Testis, meiotic
Enzymes
Collagenase Type 1 : 120 u/ml
Trypsin : 0.1%
Medium
Gey's BSS
Reproductive
Mouse
Mouse
Chen, L. , Belton, R. and Nowak, R.
Basigin-Mediated Gene Expression Changes in Mouse Uterine Stromal Cells During Implantation. ,
Endocrinology
 150 , 966 , 2009
Cells
Uterine stomal
Enzymes
Medium
DMEM/F12
Reproductive
Mouse
Mouse, embryonic
Gekas, C. , Rhodes, K. and Mikkola, H.
Isolation and Analysis of Hematopoietic Stem Cells from the Placenta. ,
J Vis Exp
 16 , e742 , 2008
Cells
Hematopoietic stem cells
Enzymes
Collagenase : 0.1%
Medium
PBS
Reproductive
Mouse
Mouse
Breault, D. , Min, I. , Carlone, D. , Farilla, L. , Ambruzs, D. , Henderson, D. , Algra, S. , Montgomery, R. , Wagers, A. and Hole, N.
Generation of mTert-GFP Mice as a Model to Identify and Study Tissue Progenitor Cells. ,
Proc Natl Acad Sci U S A
 105 , 10420 , 2008
Cells
Germ cells
Enzymes
Collagenase Type 1 : 100 u/ml
Medium
HBSS
Reproductive
Mouse
Mouse, adult, neonatal and fetal
O'Shaughnessy PJ, Fleming LM, Jackson G, Hochgeschwender U, Reed P, Baker PJ.
Adrenocorticotropic hormone directly stimulates testosterone production by the fetal and neonatal mouse testis ,
Endocrinology
 144 , 3279-84 , 2003
Cells
Testicular cells
Enzymes
Medium
DMEM/F-12
Reproductive
Mouse
Mouse, 6 day
Nalbandian Angele, Dettin Luis, Dym Martin, Ravindranath Neelakanta.
Expression of vascular endothelial growth factor receptors during male germ cell differentiation in the mouse ,
Biol Reprod
 69 , 985-94 , 2003
Cells
Sertoli
Enzymes
Medium
DMEM
Reproductive
Mouse
Mouse, male, 3.5 months old
Faldikova, L. , Diblikova, I. , Canderle, J. , Zraly, Z. , Veznik, Z. and Sulcova, A.
Effects of Nutrition, Social Factors and Chronic Stress on the Mouse Leydig Cell Testosterone Production ,
Vet Med
 46 (6) , 160 , 2001
Cells
Leydig
Enzymes
Collagenase : 0.06%
Medium
Medium E 199
Reproductive
Mouse
Mouse, 6-12 day
Eppig JJ, O'Brien MJ.
Development in vitro of mouse oocytes from primordial follicles ,
Biol Reprod
 54 , 197-207 , 1996
Cells
Oocyte-granulosa
Enzymes
Medium
Waymouth
Reproductive
Mouse
Mouse, female, 21 day, 10-12 g
Tsai, P. , Uchima, F. , Hamamoto, S. and Bern, H.
Proliferation and Differentiation of Prepubertal Mouse Vaginal Epithelial Cells In Vitro and the Specificity of Estrogen-Induced Growth Retardation ,
In Vitro Cell Dev Biol
 27A , 461 , 1991
Cells
vaginal epithelial
Enzymes
Collagenase Type 3 : 38 u/ml
Medium
DMEM
Reproductive
Mouse
Mouse (BALB/cCrgl), female, 50 - 60 days
Iguchi, T. , Uchima, F.A. , Ostrander, P. and Bern, H.
Growth of Normal Mouse Vaginal Epithelial Cells in and on Collagen Gels ,
Proc Natl Acad Sci U S A
 80 , 3743 , 1983
Cells
Epithelial, vagina
Enzymes
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.