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Tissue References Search Tool

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Prostate
Human
Human
Taylor, R. , Toivanen, R. , Frydenberg, M. , Pedersen, J. , Harewood, L. , Collins, A. , Maitland, N. and Risbridger, G.
Human Epithelial Basal Cells are Cells of Origin of Prostate Cancer, Independent of CD133 Status. ,
Stem Cells
 30 , 1087 , 2012
Cells
Prostatic cancer fibroblasts
Enzymes
Collagenase : 225 u/ml
Hyaluronidase : 125 u/ml
Medium
RPMI 1640
Prostate
Human
Human, 52-56 yr
Le Hanh, Arnold Julia T, McFann Kimberly K, Blackman Marc R.
DHT and testosterone, but not DHEA or E2, differentially modulate IGF-I, IGFBP-2, and IGFBP-3 in human prostatic stromal cells ,
Am J Physiol/Endo
 290 , E952-60 , 2006
Cells
Prostatic stromal cells
Enzymes
Medium
DMEM/F-12
Prostate
Human
Human
Nakashiro Koh-Ichi, Hara Shingo, Shinohara Yuji, Oyasu Miho, Kawamata Hitoshi, Shintani Satoru, Hamakawa Hiroyuki, Oyasu Ryoichi.
Phenotypic switch from paracrine to autocrine role of hepatocyte growth factor in an androgen-independent human prostatic carcinoma cell line, CWR22R ,
Am J Pathol
 165 , 533-40 , 2004
Cells
Prostate stromal cells
Enzymes
Medium
RPMI 1640
Prostate
Human
Human, fetal
Levine AC, Liu XH, Greenberg PD, Eliashvili M, Schiff JD, Aaronson SA, Holland JF, Kirschenbaum A.
Androgens induce the expression of vascular endothelial growth factor in human fetal prostatic fibroblasts ,
Endocrinology
 139 , 4672-8 , 1998
Cells
Prostatic fibroblasts
Enzymes
Medium
DMEM/F12
Prostate
Mouse
Mouse, 6-8 week
Burger, P. , Gupta, R. , Xiong, X. , Ontiveros, C. , Salm, S. , Moscatelli, D. and Wilson, E.
High Aldehyde Dehydrogenase Activity: A Novel Functional Marker of Murine Prostate Stem/Progenitor Cells. ,
Stem Cells
 27 , 2220-8 , 2009
Cells
Prostate epithelial/stem
Enzymes
Trypsin : 0.05%
Medium
HBSS
Prostate
Mouse
Mouse, 2 week
Imamov Otabek, Morani Andrea, Shim Gil-Jin, Omoto Yoko, Thulin-Andersson Christina, Warner Margaret, Gustafsson Jan-Ake.
Estrogen receptor beta regulates epithelial cellular differentiation in the mouse ventral prostate ,
Proc Natl Acad Sci U S A
 101 , 9375-80 , 2004
Cells
Prostatic epithelial
Enzymes
Collagenase Type 3 : 170 u/ml
Medium
DMEM
Prostate
Mouse
Mouse, male
Dubey P, Wu H, Reiter RE, Witte ON.
Alternative pathways to prostate carcinoma activate prostate stem cell antigen expression ,
Cancer Res
 61 , 3256-61 , 2001
Cells
Prostatic stem
Enzymes
Collagenase Type 1 : 170 u/ml
Medium
DMEM
Skin
Canine
Canine
Zheng, Y. , Nace, A. , Chen, W. , Watkins, K. , Sergott, L. , Homan, Y. , Vandeberg, J. , Breen, M. and Stenn, K.
Mature Hair Follicles Generated from Dissociated Cells: A Universal Mechanism of Folliculoneogenesis. ,
Dev Dyn
 239 , 2619-26 , 2010
Cells
Epidermal
Enzymes
Collagenase : 0.35%
Medium
DMEM
Skin
Goat
Goat
Deng, Y. , Hu, J. and Athanasiou, K.
Isolation and Chondroinduction of a Dermis-Isolated, Aggrecan-Sensitive Subpopulation with High Chondrogenic Potential. ,
Arthritis Rheum
 56 , 168 , 2007
Cells
Dermis isolated aggrecan sensitive
Enzymes
Medium
DMEM
Skin
Human

Human

Burja, B. , Paul, D. , Tastanova, A. , Edalat, S. , Gerber, R. , Houtman, M. , Elhai, M. , Bürki, K. , Staeger, R. , Restivo, G. , Lang, R. , Sodin-Semrl, S. , Lakota, K. , Tomšič, M. , Levesque, M. , Distler, O. , Rotar, �. , Robinson, M. and Frank-Bertoncelj, M.
An Optimized Tissue Dissociation Protocol for Single-Cell RNA Sequencing Analysis of Fresh and Cultured Human Skin Biopsies. , Front Cell Dev Biol Vol. 10 , 872688 , 2022
Cells
Skin
Enzymes
Neutral Protease : 2.4 u/ml
Collagenase Type 4 : 1,000 u/ml
Medium
RPMI
Skin
Human
Human
Tan, J. , Jaafar, F. and Makpol, S.
Proteomic Profiling of Senescent Human Diploid Fibroblasts Treated with gamma-tocotrienol. ,
BMC Complement Altern Med
 18 , 314 , 2018
Cells
Diploid fibroblasts
Enzymes
Medium
DMEM
Skin
Human
Human
Botting, Ra. , Bertram, K. , Baharlou, H. , Sandgren, K. , Fletcher, J. , Rhodes, J. , Rana, H. , Plasto, T. , Wang, X. , Lim, J. , Barnouti, L. , Kohout, M. , Papadopoulos, T. , Merten, S. , Olbourne, N. and Cunningham, A.
Phenotypic and Functional Consequences of Different Isolation Protocols on Skin Mononuclear Phagocytes. ,
J Leukoc Biol
 101 , 1393-1403 , 2017
Cells
Mononuclear phagocytes
Enzymes
Neutral Protease : 1 u/ml
Collagenase : 200 u/ml
Trypsin : 0.5%
Medium
RPMI-1640
Skin
Human
Human
Chen, Z. , Wang, X. , Jin, T. , Wang, Y. , Hong, C. , Tan, L. , Dai, T. , Wu, L. , Zhuang, Z. and Shi, C.
Increase in the Radioresistance of Normal Skin Fibroblasts but not Tumor Cells by Mechanical Injury. ,
Cell Death Dis
 8 , e2573 , 2017
Cells
Fibroblasts
Enzymes
Medium
DMEM
Skin
Human
Human
Douvaras, P. , Wang, J. , Zimmer, M. , Hanchuk, S. , O'Bara, M. , Sadiq, S. , Sim, F. , Goldman, J. and Fossati, V.
Efficient Generation of Myelinating Oligodendrocytes from Primary Progressive Multiple Sclerosis Patients by Induced Pluripotent Stem Cells. ,
Stem Cell Reports
 3 , 250 , 2014
Cells
Skin fibroblasts
Enzymes
Collagenase Type 1 : 1000 u/ml
Medium
DMEM
Skin
Human
Human
Cheuk, S. , Wiken, M. , Blomqvist, L. , Nylen, S. , Talme, T. and Stahle, M.and Eidsmo, L.
Epidermal Th22 and Tc17 Cells Form a Localized Disease Memory in Clinically Healed Psoriasis. ,
J Immunol
 192 , 3111 , 2014
Cells
Skin
Enzymes
Medium
RPMI 1640
Skin
Human
Human
Cerny, D. , Haniffa, M. , Shin, A. , Bigliardi, P. , Tan, B. , Lee, B. , Poidinger, M. , Tan, E. , Ginhoux, F. and Fink, K.
Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. ,
PLoS Pathog
 10 , e1004548 , 2014
Cells
Skin
Enzymes
Medium
RPMI
Skin
Human
Human
Carriel, V. , Garzon, I. , Jimenez, J. , Oliveira, A. , Arias-Santiago, S. , Campos, A. , Sanchez-Quevedo, M. and Alaminos, M.
Epithelial and Stromal Developmental Patterns in a Novel Substitute of the Human Skin Generated with Fibrin-Agarose Biomaterials. ,
Cells Tissues Organs
 196 , 1-12 , 2012
Cells
Fibroblasts and keratinocytes
Enzymes
Medium
DMEM
Skin
Human
Human
Karumbayaram, S. , Lee, P. , Azghadi, S. , Cooper, A. , Patterson, M. , Kohn, D. , Pyle, A. , Clark, A. , Byrne, J. , Zack, J. , Plath, K. and Lowry, W.
From Skin Biopsy to Neurons Through a Pluripotent Intermediate Under Good Manufacturing Practice Protocols ,
Stem Cells Transl Med
 1 , 36 , 2012
Cells
Fibroblasts
Enzymes
Medium
HBSS
Skin
Human
Human
Zheng, Y. , Nace, A. , Chen, W. , Watkins, K. , Sergott, L. , Homan, Y. , Vandeberg, J. , Breen, M. and Stenn, K.
Mature Hair Follicles Generated from Dissociated Cells: A Universal Mechanism of Folliculoneogenesis. ,
Dev Dyn
 239 , 2619-26 , 2010
Cells
Epidermal
Enzymes
Collagenase : 0.35%
Medium
DMEM
Skin
Human
Human
Zhang, Q. , Yamaza, T. , Kelly, AP, Shi, S. , Wang, S. , Brown, J. , Wang, L. , French, S. , Shi, S. and Le, A.
Tumor-like Stem Cells Derived from Human Keloid are Governed by the Inflammatory Niche Driven by IL-17/IL-6 Axis. ,
PLoS ONE
 4 , e7798 , 2009
Cells
Skin and keloid progenitor
Enzymes
Medium
PBS
Skin
Human
Human
Tuan, T. , Hwu, P. , Ho, W. , Yiu, P. , Chang, R. , Wysocki, A. and Benya, P.
Adenoviral Overexpression and Small Interfering RNA Suppression Demonstrate that Plasminogen Activator Inhibitor-1 Produces Elevated Collagen Accumulation in Normal and Keloid Fibroblasts. ,
Am J Pathol
 173 , 1311-25 , 2008
Cells
Fibroblasts
Enzymes
Collagenase Type 1 : 100-320 u/ml
Medium
DMEM
Skin
Human
Human, 6-12 year
Chen, F. , Zhang, W. , Bi, D. , Liu, W. , Wei, X. , Chen, F. , Zhu, L. , Cui, L. and Cao, Y.
Clonal Analysis of Nestin(-) Vimentin(+) Multipotent Fibroblasts Isolated from Human Dermis. ,
J Cell Sci
 120 , 2875 , 2007
Cells
Dermal fibroblasts
Enzymes
Medium
DMEM
Skin
Human
Human
Clark, R. , Chong, B. , Mirchandani, N. , Yamanaka, K. , Murphy, G. , Dowgiert, R. and Kupper, T.
A Novel Method for the Isolation of Skin Resident T cells from Normal and Diseased Human Skin. ,
J Invest Dermatol
 126 , 1059 , 2006
Cells
fibroblasts
Enzymes
Collagenase : 0.5%
Trypsin : 0.25%
Medium
DMEM/F12
Skin
Human
Human, adult
Wang, H. , Van Blitterswijk, C. , Bertrand-De Haas, M. , Schuurman, A. and Lamme, E.
Improved Enzymatic Isolation of Fibroblasts for the Creation of Autologous Skin Substitutes. ,
In Vitro Cell Dev Biol Anim
 40 , 268 , 2004
Cells
Dermal fibroblasts
Enzymes
Collagenase : 0.5-1.0%
Medium
DMEM
Skin
Human
Human, neonatal
Li, A. , Pouliot, N. , Redvers, R. and Kaur, P.
Extensive tissue-regenerative capacity of neonatal human keratinocytes stem cells and their progeny ,
J Clin Invest
 113 , 390-400 , 2004
Cells
Keratinocytes
Enzymes
Medium
DMEM
Skin
Human
Human
Babina Magda, Guhl Sven, Starke Andrae, Kirchhof Loreen, Zuberbier Torsten, Henz Beate M.
Comparative cytokine profile of human skin mast cells from two compartments--strong resemblance with monocytes at baseline but induction of IL-5 by IL-4 priming ,
J Leukoc Biol
 75 , 244-52 , 2004
Cells
Mast cells
Enzymes
Medium
RPMI
Skin
Human
Human
Supp Dorothy M, Wilson-Landy Kaila, Boyce Steven T.
Human dermal microvascular endothelial cells form vascular analogs in cultured skin substitutes after grafting to athymic mice ,
FASEB J
 16 , 797-804 , 2002
Cells
Keratinocytes, fibroblasts, endothelial
Enzymes
Neutral Protease : see reference
Collagenase : see reference
Medium
see reference
Skin
Human
Human
Grutzkau A, Kruger-Krasagakes S, Baumeister H, Schwarz C, Kogel H, Welker P, Lippert U, Henz BM, Moller A.
Synthesis, storage, and release of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) by human mast cells: implications for the biological significance of VEGF206 ,
Mol Biol Cell
 9 , 875-84 , 1998
Cells
Human skin mast cells
Enzymes
Collagenase Type 1 : see reference
Medium
PBS
Skin
Human
Human
Reece J C, Handley A J, Anstee E J, Morrison W A, Crowe S M, Cameron P U.
HIV-1 selection by epidermal dendritic cells during transmission across human skin ,
J Exp Med
 187 , 1623-31 , 1998
Cells
Epidermal
Enzymes
Medium
PBS
Skin
Human
Human, 18-50 years
Bell, C. and Quinton, P.
Effects of Media Buffer Systems on Growth and Electrophysiologic Characteristics of Cultured Sweat Duct Cells ,
In Vitro Cell Dev Biol
 27 , 47 , 1991
Cells
Sweat duct
Enzymes
Collagenase : 0.03%
Medium
MEM
Skin
Human
Human
Krouse, M. , Hagiqara, G. , Chen, J. , Lewiston, N. and Wine, J.
Ion Channels in Normal Human and Cystic Fibrosis Sweat Gland Cells ,
Am J Physiol
 257 , C129 , 1989
Cells
Sweat gland
Enzymes
Medium
(see reference)
Skin
Human
Human, ages 16-30
Pedersen, P.
Human Sweat Duct Cells in Primary Culture. Basic Bioelectric Properties of Cultures Derived From Normals and Patients with Cystic Fibrosis ,
In Vitro Cell Dev Biol
 25 (4) , 342 , 1989
Cells
Human sweat duct
Enzymes
Medium
RPMI 1640
(see reference)
Skin
Human
Human
Lee, C. , Carpenter, F. , Coaker, T. and Kealey, T.
The Primary Culture of Epithelia From the Secretory Coil and Collecting Duct of Normal Human and Cystic Fibrotic Eccrine Sweat Glands ,
J Cell Sci
 83 , 103 , 1986
Cells
Human sweat glands
Enzymes
Collagenase : 0.2%
Medium
HBSS
Skin
Human
Human, adult
Alitalo K, Kuismanen E, Myllyla R, Kiistala U, Asko-Seljavaara S, Vaheri A.
Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells ,
J Cell Biol
 94 , 497-505 , 1982
Cells
Epidermal keratinocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.2%
Medium
Eagle's MEM
Skin
Mouse
Mouse
Spidale, N. , Malhotra, N. , Frascoli, M. , Sylvia, K. , Miu, B. , Freeman, C. , Stadinski, B. , Huseby, E. and Kang, J.
Neonatal-Derived IL-17 Producing Dermal gd T Cells are Required to Prevent Spontaneous Atopic Dermatitis ,
Elife
 9 , 5118 , 2020
Cells
Keratinocytes
Enzymes
Medium
DMEM
Skin
Mouse
Mouse
Blanco, S. , Bandiera, R. , Popis, M. , Hussain, S. , Lombard, P. , Aleksic, J. , Sajini, A. , Tanna, H. , Cortes-Garrido, R. , Gkatza, N. , Dietmann, S. and Frye, M.
Stem Cell Function and Stress Response are Controlled by Protein Synthesis. ,
Nature
 534 , 335-40 , 2016
Cells
Keratinocytes
Enzymes
Medium
Low calcium containing media

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.