Tissue References Search Tool

Rabbit

Rabbit, New Zealand, white, male, 2-3 kg

Buxton, I. and Brunton, L.

Compartments of Cyclic AMP and Protein Kinase in Mammalian Cardiomyocytes ,
J Biol Chem
258 (17) , 10233 , 1983

Cells

Cardiomyocytes

Enzymes

Hyaluronidase : 0.5%

Medium

Eagle's MEM

Rabbit

Rabbit, adult, 2 kg

Dani, A. , Cittadini, A. , Flamini, G. , Festuccia, G. and Terranova, T.

Preparation and Some Properties of Isolated Beating Myocytes from Adult Rabbit Heart ,
J Mol Cell Cardiol
9 , 777 , 1977

Cells

Myocytes

Enzymes

Hyaluronidase : 0.007%

Medium

Kreb's Ringer

Rat

Rat, SD, 200-250 g

Farkas, A. , Acsai, K. , Nagy, N. , Toth, A. , Fulop, F. , Seprenyi, G. , Birinyi, P. , Nanasi, P. , Forster, T. , Csanady, M. , Papp, J. , Varrro, A. and Farkas, A.

Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart. ,
Br J Pharmacol
154 , 93 , 2008

Cells

Cardiomyocytes

Enzymes

Collagenase Type 1 : 0.05%

Hyaluronidase : see reference

Protease : see reference

Medium

Krebs-Henseleit

Rat

Rat, SD, male

Stagg Mark A, Coppen Steven R, Suzuki Ken, Varela-Carver Anabel, Lee Joon, Brand Nigel J, Fukushima Satsuki, Yacoub Magdi H, Terracciano Cesare M.

Evaluation of frequency, type, and function of gap junctions between skeletal myoblasts overexpressing connexin43 and cardiomyocytes: relevance to cell transplantation ,
FASEB J
20 , 744-6 , 2006

Cells

Ventricular myocytes

Enzymes

Collagenase : 0.13%

Hyaluronidase : 0.06%

Medium

DMEM

Rat

Rat, SD, 300-350 g

Niederbichler Andreas D, Hoesel Laszlo M, Westfall Margaret V, Gao Hongwei, Ipaktchi Kyros R, Sun Lei, Zetoune Firas S, Su Grace L, Arbabi Saman, Sarma J Vidya, Wang StewartC, Hemmila MarkR, Ward PeterA.

An essential role for complement C5a in the pathogenesis of septic cardiac dysfunction ,
J Exp Med
203 , 53-61 , 2006

Cells

Cardiomyocytes

Enzymes

Collagenase Type 2 : 0.05%

Hyaluronidase : 0.02%

Medium

DMEM

Rat

Rat, 50 g

Aberle II NS. and Ren J.

Experimental Assessment of the Role of Acetaldehyde in Alcoholic Cardiomyopathy ,
Biol Proced Online
5 , 1 , 2003

Cells

Ventricular myocytes

Enzymes

Collagenase : 223 u/ml

Hyaluronidase : 0.01%

Trypsin : 0.002%

Medium

Medium 199

Rat

Rat, adult, 220-280g

Gordon Jennifer M, Dusting Gregory J, Woodman Owen L, Ritchie Rebecca H.

Cardioprotective action of CRF peptide urocortin against simulated ischemia in adult rat cardiomyocytes ,
Am J Physiol Heart Circ Physiol
284 , H330-6 , 2003

Cells

Cardiomyocytes

Enzymes

Collagenase Type 2 : 178 u/ml

Hyaluronidase : 0.01%

Medium

Krebs-Henseleit, CF

Rat

Rat, SD, pregnant female

DeAlmeida, A. , Mustin, D. , Forman, M. , Brower, G. , Janicki, J. and Carver, W.

Effects of mast cells on the behavior of isolated heart fibroblasts: modulation of collagen remodeling and gene expression ,
J Cell Physiol
191 , 51 , 2002

Cells

Peritoneal mast

Enzymes

Hyaluronidase : 100 u/ml

Medium

DMEM (see reference)

Rat

Rat, adult

Ren J. and Wold LE.

Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging ,
Biol Proced Online
3 , 43 , 2001

Cells

Ventricular myocytes

Enzymes

Collagenase : 223 u/ml

Hyaluronidase : 0.01%

Trypsin : 0.002%

Medium

Tyrode solution

Rat

Rat, SD, female, 200g

Westfall M V, Rust E M, Metzger J M.

Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function ,
Proc Natl Acad Sci U S A
94 , 5444-9 , 1997

Cells

Ventricular cardiac myocytes

Enzymes

Collagenase Type 2 : 0.5%

Hyaluronidase : 0.2%

Medium

Krebs-Henseleit buffer

Rat

Rat, SD, male, 250-350 g

Kim, D. and Smith, T.W.

Temporal Variation in Contractile State And [Ca++] in Isolated Adult Rat And Guinea Pig Cardiac Myocytes , Biology of Isolated Adult Cardiac Myocytes , (Clark, Decker, Borg, eds) , Elsevier Science Publishing Co. Inc. , 370 , 1988

Cells

Myocytes, heart

Enzymes

Hyaluronidase : 0.03%

Medium

Bicarbonate-buffered medium

Rat

Rat, male/female, 200-250 g

Nag, A. and Cheng, M.

Adult Mammalian Cardiac Muscle Cells in Culture ,
Tissue Cell
13 , 515 , 1981

Cells

Muscle

Enzymes

Hyaluronidase : 0.1%

Medium

Krebs Ringer phosphate buffer, CMF

Rat

Rat, SD, adult, male, 200-250 g

Frangakis, C. , Bahl, J. , McDaniel, H. and Bressler, R.

Tolerance to Physiological Calcium by Isolated Mycocytes from the Adult Rat Heart; An Improved Cellular Preparation ,
Life Sci
27 , 815 , 1980

Cells

Myocytes

Enzymes

Hyaluronidase : 0.10%

Medium

Joklik MEM, CF

Rat

Rat, male, 200-300 g

Farmer, B. , Harris, R. , Jolly, W. , Hathaway, D. , Katzberg, A. , Watanabe, A. , Whitlow, A. and Besch, H.

Isolation and Characterization of Adult Rat Heart Cells ,
Arch Biochem Biophys
179 , 545 , 1977

Cells

Myocytes

Enzymes

Hyaluronidase : 82 u/ml

Medium

Kreb's Henseleit buffer

Rat

Rat, female, albino, 200-250 g

Nag, A. , Fischman, D. , Aumont, M. and Zak, R.

Studies of Isolated Adult Rat Heart Cells: The Surface Morphology and the Influence of Extracellular Calcium Ion Concentration on Cellular Viability ,
Tissue Cell
9 (3) , 419 , 1977

Cells

Myocytes

Enzymes

Hyaluronidase : 0.1%

Medium

Kreb's Ringers phosphate

Rat

Rat, adult, male, 150-200g

Grosso, D. , Frangakis, C. , Carlson, E. and Bressler, R.

Isolation and Characterization of Myocytes From the Adult Rat Heart ,
Prep Biochem
7 (5) , 383 , 1977

Cells

Myocytes

Enzymes

Collagenase : 0.1%

Hyaluronidase : 0.1%

Medium

MEM CF

Rat

Rat, SD, male, 150-200 g

Pretlow II, T. , Glick, M. and Reddy, W.

Separation of Beating Cardiac Myocytes from Suspensions of Heart Cells ,
Am J Pathol
67 (2) , 215 , 1972

Cells

Myocytes

Enzymes

Hyaluronidase : 0.05%

Medium

(see reference)

Rat

Rat, albino, male, 250 g

Berry, M. , Friend, D. and Scheuer, J.

Morphology and Metabolism of Intact Muscle Cells Isolated from Adult Rat Heart ,
Circ Res
26 , 679 , 1970

Cells

Myocytes

Enzymes

Hyaluronidase : 0.20%

Medium

Hank's solution, CF

Intestine

Mouse

Mouse, embryonic

Ren HJ, Zhang CL, Liu RD, Li N, Li XG, Xue HK, Guo Y, Wang ZQ, Cui J. and Ming L

Primary Cultures of Mouse Small Intestinal Epithelial Cells using the Dissociating Enzyme Type I Collagenase and Hyaluronidase. ,
Braz J Med Biol Res
50 , e5831 , 2017

Cells

Crypt

Enzymes

Collagenase Type 1 : 200 u/ml

Hyaluronidase : 100u/ml

Medium

DMEM

Bovine

Husted, R. , Hayashi, M. and Stokes, J.

Characteristics of Papillary Collecting Duct Cells in Primary Culture ,
Am J Physiol
255 , F1160 , 1988

Cells

Papillary duct

Enzymes

Hyaluronidase : 0.2%

Medium

Keri's buffer

HEPES buffered saline

Hamster

Hamster
(also rat, SD and Wistar, 150-225 g, rabbit, bovine)

Husted, R. , Hayashi, M. and Stokes, J.

Characteristics of Papillary Collecting Duct Cells in Primary Culture ,
Am J Physiol
255 , F1160 , 1988

Cells

Papillary duct

Enzymes

Hyaluronidase : 0.2%

Medium

Keri's buffer

HEPES buffered saline

Mouse

Comeau, K. , Caillon, A. , Paradis, P. and Schiffrin, E.

Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4, and CD8 T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and Lung. ,
Bio Protoc
13 , e4679 , 2023

Cells

T cells

Enzymes

Collagenase Type 1 : 0.1%

Collagenase Type 2 : 0.2%

Elastase : 2 u/ml

Soybean Trypsin Inhibitor : 0.025%

Hyaluronidase : 50 u/ml

Medium

RPMI

Rabbit

Rabbit
(also rat, SD and Wistar, 150-225 g, hamster, bovine)

Husted, R. , Hayashi, M. and Stokes, J.

Characteristics of Papillary Collecting Duct Cells in Primary Culture ,
Am J Physiol
255 , F1160 , 1988

Cells

Papillary duct

Enzymes

Hyaluronidase : 0.2%

Medium

Keri's buffer

HEPES buffered saline

Avian

Ducklings, 7 day

Lee J. , Culvenor J. , Angus P. , Smallwood R. , Nicoll A. and Locarnini S.

Duck Hepatitis B Virus Replication in Primary Bile Duct Epithelial Cells ,
J Virol
75(16) , 7651 , 2001

Cells

Hepatocytes

Enzymes

Collagenase : 0.05%

Hyaluronidase : 0.05%

Medium

DMEM/ F12

Fish

Trout 100g - 5kg

Bailey, G. , Taylor, M. and Selivonchick, D.

Aflatoxin B1 Metabolism and DNA Binding in Isolated Hepatocytes From Rainbow Trout Salmo gairdner ,
Carcinogenesis
3 , 511 , 1982

Cells

Hepatocytes

Enzymes

Hyaluronidase : 0.08%

Medium

See reference

Human

Human, fetal

Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.

Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
70 , e4181 , 2012

Cells

Liver hematopoietic

Enzymes

Collagenase Type 4 : 0.1%

Hyaluronidase : 0.1%

Deoxyribonuclease I : 2 u/ml

Medium

RPMI

Mouse

Mouse, 20-30 g

Crisp, D. and Pogson, C.

Glycolytic and Gluconeogenic Enzyme Activities in Parenchymal and Non-parenchymal Cells from Mouse Liver ,
Biochem J
126 , 1009 , 1972

Cells

Parenchymal and non-parenchymal

Enzymes

Hyaluronidase : 0.1%

Medium

Hank's w/ Insulin, CMF

Rat

Rat, SD, 500-750 g

Li, Z. , Dranoff, J. , Chan, E. , Uemura, M. and Sevigny, J.and Wells, R.

Transforming Growth Factor-beta and Substrate Stiffness Regulate Portal Fibroblast Activation in Culture. ,
Hepatol
46 , 1246-56 , 2007

Cells

Portal fibroblasts

Enzymes

Collagenase : 0.03%

Pronase : 0.033%

Hyaluronidase : 0.036%

Medium

DMEM/F-12

Rat

Rat, Fisher

Blouin, M. and Marceau, N.

Primary Culture of Fetal Rat Liver Bipotential Progenitor Cells ,
J Tiss Cul Meth
13 , 117 , 1991

Cells

Epithelial

Enzymes

Collagenase : 0.05%

Neutral Protease : 0.48%

Hyaluronidase : 0.075%

Medium

Eagles's MEM

Rat

Rat, Wistar, male, 180-210 g

De Gerlache, J. , Lans, M. , Taper, H. and Roberfroid, M.

Separate Isolation of Cells from Nodules and Surrounding Parenchyma of the Same Precancerous Rat Liver: Biochemical and Cytochemical Characterization ,
Toxicology
18 , 225 , 1980

Cells

Hepatocytes

Enzymes

Hyaluronidase : 0.02%

Medium

Kreb's buffer

Rat

Rat, Wistar, male, 200-250 g

Poli, G. , Gravela, E. , Albano, E. and Dianzani, M.

Studies on Fatty Liver with Isolated Hepatocytes: II., The Action of Carbon Tetrachloride on Lipid Peroxidation, Protein and Triglyceride Synthesis and Secretion ,
Exp Mol Pathol
30 , 116 , 1979

Cells

Hepatocytes

Enzymes

Hyaluronidase : 460 u/ml

Medium

Saline

Rat

Rat, embryos, 1-3 days

Gerschenson, L. , Berliner, J. and Davidson, M.

The Isolation and Culture of Liver Cells , Vol. 32 , 733 , 1974

Cells

Hepatocytes

Enzymes

Hyaluronidase : 0.10%

Medium

HBSS

Rat

Rat, Fischer, adult, 200-250 g

Williams, G. and Gunn, J.

Long-Term Culture of Adult Rat Liver Epithelial Cells ,
Exp Cell Res
89 , 139 , 1974

Cells

Epithelial

Enzymes

Hyaluronidase : 0.0075%

Medium

KCl-NaCl HEPES Buffer

Rat

Rat, SD, albino, male

Bonney, R. , Becker, J. , Walker, P. and Potter, V.

Primary Monolayer Cultures of Adult Rat Liver Parenchymal Cells Suitable for Study of the Regulation of Enzyme Synthesis ,
In Vitro
9 , 399 , 1974

Cells

Parenchymal

Enzymes

Hyaluronidase : 0.1%

Medium

Hank's solution, CMF