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Adipose/Fat
Bovine

Belgian Blue, 1-7 year

Mitić, R. , Cantoni, F. , Börlin, C. , Post, M. and Jackisch, L.
A Simplified and Defined Serum-free Medium for Cultivating Fat Across Species. , iScience Vol. 26 , 105822 , 2023
Cells
Stomal vascular cells
Medium
Growth medium
Adipose/Fat
Human
Human
Clark, K. , Fierro, F. , Ko, E. , Walker, N. , Arzi, B. , Tepper, C. , Dahlenburg, H. , Cicchetto, A. , Koi, A. , Marsh, L. , Murphy, W. , Fazel, N. and Borjesson, D.
Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome. ,
Stem Cell Res Ther
 8 , 69 , 2017
Cells
Adipose derived mesenchymal stem
Enzymes
Medium
PBS
Adipose/Fat
Human
Human
Hu, J. , Cao, Y. , Xie, Y. , Wang, H. , Fan, Z. , Wang, J. , Zhang, C. , Wang, J. and Wu, C. aand Wang, S.
Periodontal Regeneration in Swine After Cell Injection and Cell Sheet Transplantation of Human Dental Pulp Stem Cells Following Good Manufacturing Practice. ,
Stem Cell Res Ther
 7 , 130 , 2016
Cells
Dental pulp stem
Enzymes
Medium
HBSS
Adipose/Fat
Human
Human
Carvalho, P. , Gimble, J. , Dias, I. , Gomes, M. and Reis, R.
Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells. ,
Tiss Eng
 19 , 473-8 , 2013
Cells
Adipose stromal stem
Enzymes
Medium
DMEM/Hams F-12
Adipose/Fat
Ovine

Ovine

Mitić, R. , Cantoni, F. , Börlin, C. , Post, M. and Jackisch, L.
A Simplified and Defined Serum-free Medium for Cultivating Fat Across Species. , iScience Vol. 26 , 105822 , 2023
Cells
Stomal vascular cells
Medium
Growth medium
Adipose/Fat
Porcine

Porcine, 2-6 year

Mitić, R. , Cantoni, F. , Börlin, C. , Post, M. and Jackisch, L.
A Simplified and Defined Serum-free Medium for Cultivating Fat Across Species. , iScience Vol. 26 , 105822 , 2023
Cells
Stomal vascular cells
Medium
Growth medium
Brain
Mouse
Mouse, 0-30 day
Shrier, B. , Wilson, S. and Nirenberg, M.
Cultured Cell Systems and Methods for Neurobiology , Vol. 32 , 765 , 1974
Cells
Neural
Enzymes
Medium
BSS
Colon
Human
Human
Rollo, B. , Zhang, D. , Stamp, L. , Menheniott, T. , Stathopoulos, L. , Denham, M. , Dottori, M. , King, S. , Hutson, J. and Newgreen, D.
Enteric Neural Cells from Hirschsprung Disease Patients Form Ganglia in Autologous Aneuronal Colon ,
Cell Mol Gastroenterol Hepatol
 2 , 92 , 2016
Cells
Colon enteric neural
Enzymes
Medium
Ham's F-12
Endothelial
Human
Human
Lewis, L.J. , Haok, J.C. , Maca, R.D. and Fry, G.L.
Replication of Human Endothelial Cells in Culture ,
Science
 181 , 452 , 1973
Cells
Umbilical cord
Enzymes
Trypsin NF 1:250 : 0.125%
Medium
Saline, normal
Endothelial
Human
Human
Fryer, D.G. , Birnbaum, G. and Luttrell, C.N.
Human Endothelium in Cell Culture ,
J Atheroscler Res
 6 , 151 , 1966
Cells
Umbilical cord
Enzymes
Medium
CMF solution
Heart
Chicken
Chick, 8 day embryo
(also rat, neonate and mouse)
Gross, W. , Schopf-Ebner, E. and Bucher, O.
Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells ,
Exp Cell Res
 53 , 1 , 1968
Cells
Myocytes
Enzymes
Medium
Rinaldini's buffer solution, CF
Heart
Mouse
Mouse
(also rat, neonate and chick, 8 day embryo)
Gross, W. , Schopf-Ebner, E. and Bucher, O.
Technique for the Preparation of Homogeneous Cultures of Isolated Heart Muscle Cells ,
Exp Cell Res
 53 , 1 , 1968
Cells
Myocytes
Enzymes
Medium
Rinaldini's buffer solution, CF
Intestine
Mouse
Mouse, P4
Hotta, R. , Stamp, L. , Foong, J. , McConnell, S. , Bergner, A. , Anderson, R. , Enomoto, H. , Newgreen, D. , Obermayr, F. , Furness, J. and Young, H.
Transplanted Progenitors Generate Functional Enteric Neurons in the Postnatal Colon. ,
J Clin Invest
 123 , 1182 , 2013
Cells
Enteric neural crest progenitors
Enzymes
Medium
DMEM/F12
Mammary
Guinea-Pig
Guinea-pig, pregnant, 4-10 day
Turba, F. and Hilpert, N.
Secretion and Resorption of Proteins by Isolated Mammary Gland Cells. German ,
Biochem Z
 334 , 501 , 1961
Cells
Mammary gland
Enzymes
Medium
Dulbecco phosphate
Mammary
Mouse
Mouse, lactating, 14 day
Kopelovich, L. , Abraham, S. , McGrath, H. , DeOme, K. and Chaikoff, I.
Metabolic Characteristics of a Naturally Ocurring Preneoplastic Tissue. I. Glycolytic Enzyme Activators of Hyperplastic Alveolar Nodule Outgrowths and Adenocarcinomas of Mouse Mammary Gland ,
Cancer Res
 26 , 1534 , 1966
Cells
Mammary
Enzymes
Medium
HBSS
Muscle
Rat
Rat, 3-4 day
Kasten, F.
Rat Myocardial Cells In Vitro: Mitosis and Differentiated Properties ,
In Vitro
 8 , 128 , 1972
Cells
Myocardial
Enzymes
Trypsin NF 1:250 : 0.125%
Medium
HBSS CMF
Muscle
Rat
Rat, Wistar, 3-10 day
Harary, I. and Farley, B.
In Vitro Studies on Single Beating Rat Heart Cells ,
Exp Cell Res
 29 , 451 , 1963
Cells
Heart
Enzymes
Trypsin NF 1:250 : 250: 0.1%
Medium
Saline A
(See reference)
Neural
Avian
Quail and Chicken, embryonic
Rollo, B. , Zhang, D. , Simkin, J. , Menheniott, T. and Newgreen, D.
Why are Enteric Ganglia so Small? Role of Differential Adhesion of Enteric Neurons and Enteric Neural Crest Cells. ,
F1000Res
 4 , 113 , 2015
Cells
Midgut DRG
Enzymes
Medium
Ham's F12
Neural
Mouse
Mouse
Salisbury, E. , Lazard, Z. , Ubogu, E. , Davis, A. and Olmsted-Davis, E.
Transient Brown Adipocyte-Like Cells Derive from Peripheral Nerve Progenitors in Response to Bone Morphogenetic Protein 2. ,
Stem Cells Transl Med
 1 , 874-85 , 2012
Cells
Nerve progenitors
Enzymes
Medium
HBSS
Neural
Mouse
Mouse, 0-30 day
Shrier, B. , Wilson, S. and Nirenberg, M.
Cultured Cell Systems and Methods for Neurobiology , Vol. 32 , 765 , 1974
Cells
Neural
Enzymes
Medium
BSS
Skin
Human
Human
Karumbayaram, S. , Lee, P. , Azghadi, S. , Cooper, A. , Patterson, M. , Kohn, D. , Pyle, A. , Clark, A. , Byrne, J. , Zack, J. , Plath, K. and Lowry, W.
From Skin Biopsy to Neurons Through a Pluripotent Intermediate Under Good Manufacturing Practice Protocols ,
Stem Cells Transl Med
 1 , 36 , 2012
Cells
Fibroblasts
Enzymes
Medium
HBSS
Stem
Human
Human
Clark, K. , Fierro, F. , Ko, E. , Walker, N. , Arzi, B. , Tepper, C. , Dahlenburg, H. , Cicchetto, A. , Koi, A. , Marsh, L. , Murphy, W. , Fazel, N. and Borjesson, D.
Human and Feline Adipose-Derived Mesenchymal Stem Cells Have Comparable Phenotype, Immunomodulatory Functions, and Transcriptome. ,
Stem Cell Res Ther
 8 , 69 , 2017
Cells
Adipose derived mesenchymal stem
Enzymes
Medium
PBS
Stem
Human
Human
Lee, S. , Park, B. , Kim, J. , Jekarl, D. , Choi, H. , Lee, S. , Kim, M. , Kim, Y. and Park, M.
The Effect of Fibroblast Growth Factor on Distinct Differentiation Potential of Cord Blood-Derived Unrestricted Somatic stem Cells and Wharton's Jelly-Derived Mesenchymal Stem/Stromal Cells. ,
Cytotherapy
 17 , 1723-31 , 2015
Cells
Wharton jelly mesenchymal stem
Enzymes
Medium
PBS
Stem
Human
Human
Carvalho, P. , Gimble, J. , Dias, I. , Gomes, M. and Reis, R.
Xenofree Enzymatic Products for the Isolation of Human Adipose-Derived Stromal/Stem Cells. ,
Tiss Eng
 19 , 473-8 , 2013
Cells
Adipose stromal stem
Enzymes
Medium
DMEM/Hams F-12
Stem
Mouse
Mouse, P4
Hotta, R. , Stamp, L. , Foong, J. , McConnell, S. , Bergner, A. , Anderson, R. , Enomoto, H. , Newgreen, D. , Obermayr, F. , Furness, J. and Young, H.
Transplanted Progenitors Generate Functional Enteric Neurons in the Postnatal Colon. ,
J Clin Invest
 123 , 1182 , 2013
Cells
Enteric neural crest progenitors
Enzymes
Medium
DMEM/F12
Stem
Mouse
Mouse
Salisbury, E. , Lazard, Z. , Ubogu, E. , Davis, A. and Olmsted-Davis, E.
Transient Brown Adipocyte-Like Cells Derive from Peripheral Nerve Progenitors in Response to Bone Morphogenetic Protein 2. ,
Stem Cells Transl Med
 1 , 874-85 , 2012
Cells
Nerve progenitors
Enzymes
Medium
HBSS
Tumor
Mouse
Mouse, lactating, 14 day
Kopelovich, L. , Abraham, S. , McGrath, H. , DeOme, K. and Chaikoff, I.
Metabolic Characteristics of a Naturally Ocurring Preneoplastic Tissue. I. Glycolytic Enzyme Activators of Hyperplastic Alveolar Nodule Outgrowths and Adenocarcinomas of Mouse Mammary Gland ,
Cancer Res
 26 , 1534 , 1966
Cells
Mammary
Enzymes
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.