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Tissue References Search Tool

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Adipose/Fat
Rat
Rat, SD, male, 4-7 weeks
Omatsu-Kanbe, M. and Matsuura, H.
Inhibition of Store-operated Ca2+ Entry by Extracellular ATP in Rat Brown Adipocytes ,
J Physiol
 521 (3) , 601 , 1999
Cells
Brown adipocytes
Enzymes
Medium
DMEM
Adrenal
Rat
Rat
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Rat
Rat
Gilabert, J, Montalvo, G. and Artalejo A.
Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assays ,
Nat Protoc
 , 294 , 2006
Cells
Chromaffin
Enzymes
Medium
HBSS
Adrenal
Rat
Rat, SD
Gilabert, J.
Necessary Conditions to Maintain Rat Adrenal Chromaffin Cells in Primary Culture , Cell Biology of the Chromaffin Cell , Borges, R. and Gandia, L. , Instituto Teofilo Hernando , 2004
Cells
Chromaffin cells
Enzymes
Medium
HBSS
Adrenal
Rat
Rat, Wistar, newborn
Zhang L, Castell A, Avila E, Drucker-Colín R, Escobar A.
Immunocytochemical, ultrastructural and neurochemical evidences on synaptogenesis and dopamine release of rat chromaffin cells co-cultured with striatal neurons ,
J Neuropathol Exp Neurol
 59 , 170-4 , 2000
Cells
Chromaffin cells
Enzymes
Medium
DMEM
Adrenal
Rat
Rat, Fischer, male, 10-16 weeks
Roskelley, C.D. and Auersperg, N.
Density Separation of Rat Adrenocortical Cells: Morphology, Steroidogenesis, and P-450scc Expression in Primary Culture ,
In Vitro Cell Dev Biol
 26 , 493 , 1990
Cells
Adrenocortical
Enzymes
Medium
BSS
Adrenal
Rat
Rat, Fischer, male, 1-10 months
Leonard, R.K. , Auersperg, N. and Parkes, C.O.
Ascorbic Acid Accumulation by Cultured Rat Adrenocortical Cells ,
In Vitro
 19 , 46 , 1983
Cells
Adrenocortical
Enzymes
Medium
BSS
Adrenal
Rat
Rat, SD, male, 400-450 g
Li, C.H. , Ng, T.B. and Cheng, C.H.K.
Melanotropins: Aldosterone- and Corticosterone-Stimulating Activity in Isolated Rat Adrenal Cells ,
Int J Pept Protein Res
 19 , 361 , 1982
Cells
Decapular
Capsular
Glomerulosa
Enzymes
Medium
Medium 199
Adrenal
Rat
Rat , SD, female, 200 g
Braley, L. , Williams, G. and Bradwin, G.
The Effect of Unit Gravity Sedimentation on Adrenal Steroidogenesis by Isolated Rat Glomerulosa and Fasciculata Cells ,
Endocrinology
 106 (1) , 50 , 1980
Cells
Glomerulosa
Enzymes
Medium
Kreb's
Brain
Rat
Rat, P0-P15
Foo, L.
Purification of Rat and Mouse Astrocytes by Immunopanning. ,
Cold Spring Harb. Protoc.
 2013 , 421-32 , 2013
Cells
Astrocytes
Enzymes
Papain : 5 u/ml
Medium
DPBS
Brain
Rat
Rat, SD, E18
Behar, T, Smith, S, Kennedy, R, Mckenzie, J, Maric, I. and Barker, J
GABA(B) receptors mediate motility signals for migrating embryonic cortical cells ,
Cereb Cortex
 11 , 744-53 , 2001
Cells
Cortical cells
Enzymes
Papain : 20 u/ml
Medium
EBSS
Brain
Rat
Rat, SD, 5 day
Mattson, M. and Kater, S.
Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions ,
Brain Res
 490 , 110 , 1989
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.2%
Deoxyribonuclease I : 10 ug/ml
Medium
HBSS
Brain
Rat
Rat, SD, fetal
Peterfreund, R. and Vale, W.
High Molecular Weight Somatostatin Secretion by Cultured Rat Brain Cell ,
Brain Res
 239 , 463 , 1982
Cells
Cerebral cortex
Hypothalmus
Enzymes
Medium
HEPES
Epithelial
Rat
Rat, male
Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M.
Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair ,
J Cell Sci
 112 , 243 , 1999
Cells
Alveolar epithelial
Enzymes
Elastase : 40 u/ml
Medium
DMEM
Epithelial
Rat
Rat, embryonic
Shannon JM, Gebb SA. and Nielsen LD
Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture ,
Development
 126 , 1675 , 1999
Cells
Tracheal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Rat
Rat, SD, 200 g
Yassin, R. , Clearfield, H. , Katz, S. and Murthy, S.
Gastrin Induction of mRNA Expression in Rat Colonic Epithelium In Vitro ,
Peptides
 12 , 63 , 1991
Cells
Colon
Enzymes
Deoxyribonuclease I : 10 µg/ml
Medium
(see reference)
Eye
Rat

Rat, SD, 1-2 day

Lian, W. , Hu, X. , Zhang, J. , Wu, Y. , Zhao, N. , Ma, H. , He, H. and Lu, Q.
Fucoxanthin Protects Retinal Ganglion Cells and Promotes Parkin-mediated Mitophagy against Glutamate Excitotoxicity. ,
Neuroreport
 34 , 385-394 , 2023
Cells
Retinal ganglion
Enzymes
Papain : 0.5%
Medium
MEM
Eye
Rat
Rat, Wister, 3-30 wk
Cameron, M. , Kekesi, O. , Morley, J. , Tapson, J. , Breen, P. , Van Schaik, A. and Buskila, Y.
Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue. ,
PLoS ONE
 11 , e0155468 , 2016
Cells
Retinal
Enzymes
Papain : 30 u/ml
Medium
EBSS
Heart
Rat
Rat, adult
Zhang, Y. , Wang, Y. , Yanni, J. , Qureshi, M. , Logantha, S. , Kassab, S. , Boyett, M. , Gardiner, N. , Sun, H. , Howarth, F. and Dobrzynski, H.
Electrical Conduction System Remodeling in Streptozotocin-Induced Diabetes Mellitus Rat Heart. ,
Front Physiol
 10 , 826 , 2019
Cells
Sinoatrial nodal
Enzymes
Collagenase Type 4 : 220 u/ml
Elastase : 1.4 u/ml
Protease : 0.45 u/ml
Medium
Tyrode solution
Heart
Rat
Rat, SD, 175-200g
Tardif Annie, Julien Nathalie, Chiasson Jean-Louis, Coderre Lise.
Stimulation of glucose uptake by chronic vanadate pretreatment in cardiomyocytes requires PI 3-kinase and p38 MAPK activation ,
Am J Physiol/Endo
 284 , E1055-64 , 2003
Cells
Cardiomyocytes
Enzymes
Medium
Krebs-Ringer
Heart
Rat
Rat, SD, adult female
Puri S, Bansal DD, Uskokovic MR. and MacGregor RR.
Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)(2)D(3) ,
Am J Physiol/Endo
 278(2) , E293 , 2000
Cells
Heart microvascular cells
Enzymes
CLSPA : 250u/ml
Papain : 5 u/ml
Elastase : 0.8 u/ml
Medium
L-15
Heart
Rat
Rat
Zilberman, A. , Dave, V. , Miano, J. , Olson, E. and Periasamy, M.
Evolutionary Conserved Promoter Region Containing CArg-Like Elements is Crucial for Smooth Muscle Myosin Heavy Chain Gene Expression ,
Circ Res
 82 , 566 , 1998
Cells
Thoracic aorta, smooth muscle
Enzymes
Elastase : 0.02%
Medium
(see reference)
Intestine
Rat
Rat, SD, embryonic
Bixby, S. , Kruger, G. , Mosher, J. , Joseph, N. and Morrison, S.
Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity. ,
Neuron
 35 , 643 , 2002
Cells
Sciatic nerve and gut neural crest stem
Enzymes
Trypsin : 0.005%
Medium
HBSS
Kidney
Rat
Rat, SD, male
Schafer, J. , Watkins, M. , Li, L. , Herter, P. , Haxelmans, S. and Schlatter, E.
A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments ,
Am J Physiol
 273 , F650 , 1997
Cells
Nephron segments
Enzymes
Medium
Eagle's MEM
Liver
Rat
Rat, SD, 500-700g
Perepelyuk, M. , Terajima, M. , Wang, A. , Georges, P. , Janmey, P. , Yamauchi, M. and Wells, R.
Hepatic Stellate Cells and Portal Fibroblasts are the Major Cellular Sources of Collagens and Lysyl Oxidases in Normal Liver and Early After Injury. ,
Am J Physiol Gastrointest Liver Physiol
 304 , G605 , 2013
Cells
Hepatocytes and sinusoidal endothelial
Enzymes
Medium
HBSS
Liver
Rat
Rat, adult
Jensen C. , Jauho E. , Santoni-Rugiu E. , Holmskov U. , Teisner B. , Tygstrup N. and Bisgaard H.
Transit-amplifying Ductular (oval) Cells and Their Hepatocytic Progeny are Characterized by a Novel and Distinctive Expression of Delta-like Protein/preadipocyte Factor 1/fetal Antigen 1 ,
Am J Pathol
 164(4) , 1347 , 2004
Cells
Hepatocytes
Enzymes
Pronase : 1%
Medium
Williams E
Lung
Rat
Rat, SD, 250-300g
Chen J. , Chen Z. , Narasaraju T. , Jin N. and Liu L.
Isolation of Highly Pure Alveolar Epithelial Type I and Type II Cells from Rat Lungs ,
Lab Invest
 84 , 727 , 2004
Cells
Alveolar epithelial
Enzymes
Elastase : 3-4.5 u/ml
Medium
RPMI 1640
Lung
Rat
Rat, SD, 125-150 g
Liebler, J. , Borok, Z. , Li, X. , Zhou, B. , Sandoval, A. and Kim, K.and Crandall, E.
Alveolar Epithelial Type I Cells Express Beta 2-Adrenergic Receptors and G-protein Receptor Kinase 2. ,
J Histochem Cytochem
 52 , 759 , 2004
Cells
Alveolar type I & II
Enzymes
Elastase : 2.5-8 u/ml
Medium
DMEM/F12
Lung
Rat
Rat, SD, 200-225 g
Sunil VR, Connor AJ, Guo Y, Laskin JD. and Laskin DL
Activation of type II alveolar epithelial cells during acute endotoxemia ,
Am J Physiol Lung Cell Mol Physiol
 282 , L872 , 2002
Cells
Type II alveolar epithelial cells
Enzymes
Elastase : 4.2 u/ml
Medium
DMEM
Lung
Rat
Rat, SD, male, adult
Kemp, P. , Kim, K. , Borok. and Crandall, E.
Re-evaluating the Na+ Conductance of Adult Rat Alveolar Type II Pneumocytes: Evidence for the Involvement of cGMP-Activated Cation Channels ,
J Physiol
 536 (3) , 693 , 2001
Cells
Alveolar
Enzymes
Elastase : 2.0 - 2.5 u/ml
Medium
DMEM/Ham's F-12
(see reference)
Lung
Rat
Rat, Lewis, male, 200-250g
Steinmuller C, Franke-Ullmann G, Lohmann-Matthes ML, Emmendorffer A.
Local activation of nonspecific defense against a respiratory model infection by application of interferon-gamma: comparison between rat alveolar and interstitial lung macrophages ,
Am J Respir Cell Mol Biol
 22 , 481-90 , 2000
Cells
Interstitial lung macrophages
Enzymes
Medium
RPMI-1640
Lung
Rat
Rat, male
Planus, E. , Galiacy, S. , Matthay, M. , Laurent, V. , Gavrilovic, J. , Murphy, G. , Clerici, C. , Isabey, D. , Lafuma, C. and d'Ortho, M.
Role of Collagenase in Mediating in Vitro Alveolar Epithelial Wound Repair ,
J Cell Sci
 112 , 243 , 1999
Cells
Alveolar epithelial
Enzymes
Elastase : 40 u/ml
Medium
DMEM
Lung
Rat
Rat, male, 200-250g
Bakhramov, A, Evans, M. and Kozlowski, R
Differential effects of hypoxia on the intracellular Ca2+ concentration of myocytes isolated from different regions of the rat pulmonary arterial tree ,
Exp Physiol
 83 , 337-47 , 1998
Cells
Pulmonary arterial myocytes
Enzymes
Collagenase : 0.15%
Papain : 0.15%
Elastase : 0.05%
Medium
PBS
Lung
Rat
Rat, SD, male, 125-175 g
Kim, K. , Suh, D. , Lubman, R. , Danto, S. , Borok, Z. and Crandall, E.
Studies on the Mechanisms of Active Ion Fluxes Across Alveolar Epithelial Cell Monolayers ,
J Tiss Cul Meth
 14 , 187 , 1992
Cells
Alveolar epithelial
Enzymes
Elastase : 2 u/ml
Medium
EBSS
Lung
Rat
Rat, Fischer 344, male
Mangum, J. , Everitt, J. , Bonner, J. , Moore, L. and Brody, A.
Co-Culture of Primary Pulmonary Cells to Model Alveolar Injury and Translocation of Proteins ,
In Vitro Cell Dev Biol
 26 , 1135 , 1990
Cells
Alveolar type II pneumocytes
Enzymes
Elastase : 40 u/ml
Medium
HEPES buffer
(see reference)
Lung
Rat
Rat, SD, male, 250-400 g
Ma, J. , LaCagnin, L. , Bowman, L. and Miles, P.
Carbon Tetrachloride Inhibits Synthesis of Pulmonary Surfactant Disaturated Phosphatidylcholines and ATP Production in Alveolar Type II Cells ,
Biochim Biophys Acta
 1003 , 136 , 1989
Cells
Alveolar type II
Enzymes
Elastase : 40 u/ml
Medium
Phosphate-buffered medium
(see reference)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.