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Tissue References Search Tool

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Adipose/Fat
Rat
Rat, SD, male, 4-7 weeks
Omatsu-Kanbe, M. and Matsuura, H.
Inhibition of Store-operated Ca2+ Entry by Extracellular ATP in Rat Brown Adipocytes ,
J Physiol
 521 (3) , 601 , 1999
Cells
Brown adipocytes
Enzymes
Medium
DMEM
Adrenal
Rat
Rat
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Rat
Rat
Gilabert, J, Montalvo, G. and Artalejo A.
Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assays ,
Nat Protoc
 , 294 , 2006
Cells
Chromaffin
Enzymes
Medium
HBSS
Adrenal
Rat
Rat, SD
Gilabert, J.
Necessary Conditions to Maintain Rat Adrenal Chromaffin Cells in Primary Culture , Cell Biology of the Chromaffin Cell , Borges, R. and Gandia, L. , Instituto Teofilo Hernando , 2004
Cells
Chromaffin cells
Enzymes
Medium
HBSS
Adrenal
Rat
Rat, Wistar, newborn
Zhang L, Castell A, Avila E, Drucker-Colín R, Escobar A.
Immunocytochemical, ultrastructural and neurochemical evidences on synaptogenesis and dopamine release of rat chromaffin cells co-cultured with striatal neurons ,
J Neuropathol Exp Neurol
 59 , 170-4 , 2000
Cells
Chromaffin cells
Enzymes
Medium
DMEM
Adrenal
Rat
Rat, Fischer, male, 10-16 weeks
Roskelley, C.D. and Auersperg, N.
Density Separation of Rat Adrenocortical Cells: Morphology, Steroidogenesis, and P-450scc Expression in Primary Culture ,
In Vitro Cell Dev Biol
 26 , 493 , 1990
Cells
Adrenocortical
Enzymes
Medium
BSS
Adrenal
Rat
Rat, Fischer, male, 1-10 months
Leonard, R.K. , Auersperg, N. and Parkes, C.O.
Ascorbic Acid Accumulation by Cultured Rat Adrenocortical Cells ,
In Vitro
 19 , 46 , 1983
Cells
Adrenocortical
Enzymes
Medium
BSS
Adrenal
Rat
Rat, SD, male, 400-450 g
Li, C.H. , Ng, T.B. and Cheng, C.H.K.
Melanotropins: Aldosterone- and Corticosterone-Stimulating Activity in Isolated Rat Adrenal Cells ,
Int J Pept Protein Res
 19 , 361 , 1982
Cells
Decapular
Capsular
Glomerulosa
Enzymes
Medium
Medium 199
Adrenal
Rat
Rat , SD, female, 200 g
Braley, L. , Williams, G. and Bradwin, G.
The Effect of Unit Gravity Sedimentation on Adrenal Steroidogenesis by Isolated Rat Glomerulosa and Fasciculata Cells ,
Endocrinology
 106 (1) , 50 , 1980
Cells
Glomerulosa
Enzymes
Medium
Kreb's
Brain
Rat
Rat, P0-P15
Foo, L.
Purification of Rat and Mouse Astrocytes by Immunopanning. ,
Cold Spring Harb. Protoc.
 2013 , 421-32 , 2013
Cells
Astrocytes
Enzymes
Papain : 5 u/ml
Medium
DPBS
Brain
Rat
Rat, SD, E18
Behar, T, Smith, S, Kennedy, R, Mckenzie, J, Maric, I. and Barker, J
GABA(B) receptors mediate motility signals for migrating embryonic cortical cells ,
Cereb Cortex
 11 , 744-53 , 2001
Cells
Cortical cells
Enzymes
Papain : 20 u/ml
Medium
EBSS
Brain
Rat
Rat, SD, 5 day
Mattson, M. and Kater, S.
Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions ,
Brain Res
 490 , 110 , 1989
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.2%
Deoxyribonuclease I : 10 ug/ml
Medium
HBSS
Brain
Rat
Rat, SD, fetal
Peterfreund, R. and Vale, W.
High Molecular Weight Somatostatin Secretion by Cultured Rat Brain Cell ,
Brain Res
 239 , 463 , 1982
Cells
Cerebral cortex
Hypothalmus
Enzymes
Medium
HEPES
Epithelial
Rat
Rat, embryonic
Shannon JM, Gebb SA. and Nielsen LD
Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture ,
Development
 126 , 1675 , 1999
Cells
Tracheal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Rat
Rat, 4-14 week
Wang N, Koutz CA. and Anderson RE.
A method for the isolation of retinal pigment epithelial cells from adult rats ,
Inv Ophthal Visual Sci
 34(1) , 101 , 1993
Cells
Retinal pigment epithelial cells
Enzymes
Collagenase Type 1 : 65 u/ml
Hyaluronidase : 220 u/ml
Medium
CF Hanks with EDTA
Epithelial
Rat
Rat, SD, 200 g
Yassin, R. , Clearfield, H. , Katz, S. and Murthy, S.
Gastrin Induction of mRNA Expression in Rat Colonic Epithelium In Vitro ,
Peptides
 12 , 63 , 1991
Cells
Colon
Enzymes
Deoxyribonuclease I : 10 µg/ml
Medium
(see reference)
Epithelial
Rat
Rat, Fischer, male, 4-6 weeks
Babcock, M. , Marino, M. , Gunning, W. and Stoner, G.
Clonal Growth and Serial Propagation of Rat Esophageal Epithelial Cells ,
In Vitro
 19 , 403 , 1983
Cells
Epithelial
Esophagus
Enzymes
Hyaluronidase : 0.1%
Medium
HEPES BSS
Epithelial
Rat
Rat, Fischer, adult, 200-250 g
Williams, G. and Gunn, J.
Long-Term Culture of Adult Rat Liver Epithelial Cells ,
Exp Cell Res
 89 , 139 , 1974
Cells
Epithelial
Enzymes
Hyaluronidase : 0.0075%
Medium
KCl-NaCl HEPES Buffer
Eye
Rat

Rat, SD, 1-2 day

Lian, W. , Hu, X. , Zhang, J. , Wu, Y. , Zhao, N. , Ma, H. , He, H. and Lu, Q.
Fucoxanthin Protects Retinal Ganglion Cells and Promotes Parkin-mediated Mitophagy against Glutamate Excitotoxicity. ,
Neuroreport
 34 , 385-394 , 2023
Cells
Retinal ganglion
Enzymes
Papain : 0.5%
Medium
MEM
Eye
Rat
Rat, Wister, 3-30 wk
Cameron, M. , Kekesi, O. , Morley, J. , Tapson, J. , Breen, P. , Van Schaik, A. and Buskila, Y.
Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue. ,
PLoS ONE
 11 , e0155468 , 2016
Cells
Retinal
Enzymes
Papain : 30 u/ml
Medium
EBSS
Eye
Rat
Rat, 4-14 week
Wang N, Koutz CA. and Anderson RE.
A method for the isolation of retinal pigment epithelial cells from adult rats ,
Inv Ophthal Visual Sci
 34(1) , 101 , 1993
Cells
Retinal pigment epithelial cells
Enzymes
Collagenase Type 1 : 65 u/ml
Hyaluronidase : 220 u/ml
Medium
CF Hanks with EDTA
Heart
Rat
Rat, SD, 200-250 g
Farkas, A. , Acsai, K. , Nagy, N. , Toth, A. , Fulop, F. , Seprenyi, G. , Birinyi, P. , Nanasi, P. , Forster, T. , Csanady, M. , Papp, J. , Varrro, A. and Farkas, A.
Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart. ,
Br J Pharmacol
 154 , 93 , 2008
Cells
Cardiomyocytes
Enzymes
Hyaluronidase : see reference
Protease : see reference
Medium
Krebs-Henseleit
Heart
Rat
Rat, SD, male
Stagg Mark A, Coppen Steven R, Suzuki Ken, Varela-Carver Anabel, Lee Joon, Brand Nigel J, Fukushima Satsuki, Yacoub Magdi H, Terracciano Cesare M.
Evaluation of frequency, type, and function of gap junctions between skeletal myoblasts overexpressing connexin43 and cardiomyocytes: relevance to cell transplantation ,
FASEB J
 20 , 744-6 , 2006
Cells
Ventricular myocytes
Enzymes
Collagenase : 0.13%
Hyaluronidase : 0.06%
Medium
DMEM
Heart
Rat
Rat, SD, 300-350 g
Niederbichler Andreas D, Hoesel Laszlo M, Westfall Margaret V, Gao Hongwei, Ipaktchi Kyros R, Sun Lei, Zetoune Firas S, Su Grace L, Arbabi Saman, Sarma J Vidya, Wang StewartC, Hemmila MarkR, Ward PeterA.
An essential role for complement C5a in the pathogenesis of septic cardiac dysfunction ,
J Exp Med
 203 , 53-61 , 2006
Cells
Cardiomyocytes
Enzymes
Medium
DMEM
Heart
Rat
Rat, 50 g
Aberle II NS. and Ren J.
Experimental Assessment of the Role of Acetaldehyde in Alcoholic Cardiomyopathy ,
Biol Proced Online
 5 , 1 , 2003
Cells
Ventricular myocytes
Enzymes
Collagenase : 223 u/ml
Hyaluronidase : 0.01%
Trypsin : 0.002%
Medium
Medium 199
Heart
Rat
Rat, adult, 220-280g
Gordon Jennifer M, Dusting Gregory J, Woodman Owen L, Ritchie Rebecca H.
Cardioprotective action of CRF peptide urocortin against simulated ischemia in adult rat cardiomyocytes ,
Am J Physiol Heart Circ Physiol
 284 , H330-6 , 2003
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 178 u/ml
Hyaluronidase : 0.01%
Medium
Krebs-Henseleit, CF
Heart
Rat
Rat, SD, 175-200g
Tardif Annie, Julien Nathalie, Chiasson Jean-Louis, Coderre Lise.
Stimulation of glucose uptake by chronic vanadate pretreatment in cardiomyocytes requires PI 3-kinase and p38 MAPK activation ,
Am J Physiol/Endo
 284 , E1055-64 , 2003
Cells
Cardiomyocytes
Enzymes
Medium
Krebs-Ringer
Heart
Rat
Rat, SD, pregnant female
DeAlmeida, A. , Mustin, D. , Forman, M. , Brower, G. , Janicki, J. and Carver, W.
Effects of mast cells on the behavior of isolated heart fibroblasts: modulation of collagen remodeling and gene expression ,
J Cell Physiol
 191 , 51 , 2002
Cells
Peritoneal mast
Enzymes
Hyaluronidase : 100 u/ml
Medium
DMEM (see reference)
Heart
Rat
Rat, adult
Ren J. and Wold LE.
Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging ,
Biol Proced Online
 3 , 43 , 2001
Cells
Ventricular myocytes
Enzymes
Collagenase : 223 u/ml
Hyaluronidase : 0.01%
Trypsin : 0.002%
Medium
Tyrode solution
Heart
Rat
Rat, SD, female, 200g
Westfall M V, Rust E M, Metzger J M.
Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function ,
Proc Natl Acad Sci U S A
 94 , 5444-9 , 1997
Cells
Ventricular cardiac myocytes
Enzymes
Medium
Krebs-Henseleit buffer
Heart
Rat
Rat, SD, male, 250-350 g
Kim, D. and Smith, T.W.
Temporal Variation in Contractile State And [Ca++] in Isolated Adult Rat And Guinea Pig Cardiac Myocytes , Biology of Isolated Adult Cardiac Myocytes , (Clark, Decker, Borg, eds) , Elsevier Science Publishing Co. Inc. , 370 , 1988
Cells
Myocytes, heart
Enzymes
Hyaluronidase : 0.03%
Medium
Bicarbonate-buffered medium
Heart
Rat
Rat, male/female, 200-250 g
Nag, A. and Cheng, M.
Adult Mammalian Cardiac Muscle Cells in Culture ,
Tissue Cell
 13 , 515 , 1981
Cells
Muscle
Enzymes
Hyaluronidase : 0.1%
Medium
Krebs Ringer phosphate buffer, CMF
Heart
Rat
Rat, SD, adult, male, 200-250 g
Frangakis, C. , Bahl, J. , McDaniel, H. and Bressler, R.
Tolerance to Physiological Calcium by Isolated Mycocytes from the Adult Rat Heart; An Improved Cellular Preparation ,
Life Sci
 27 , 815 , 1980
Cells
Myocytes
Enzymes
Hyaluronidase : 0.10%
Medium
Joklik MEM, CF
Heart
Rat
Rat, male, 200-300 g
Farmer, B. , Harris, R. , Jolly, W. , Hathaway, D. , Katzberg, A. , Watanabe, A. , Whitlow, A. and Besch, H.
Isolation and Characterization of Adult Rat Heart Cells ,
Arch Biochem Biophys
 179 , 545 , 1977
Cells
Myocytes
Enzymes
Hyaluronidase : 82 u/ml
Medium
Kreb's Henseleit buffer
Heart
Rat
Rat, female, albino, 200-250 g
Nag, A. , Fischman, D. , Aumont, M. and Zak, R.
Studies of Isolated Adult Rat Heart Cells: The Surface Morphology and the Influence of Extracellular Calcium Ion Concentration on Cellular Viability ,
Tissue Cell
 9 (3) , 419 , 1977
Cells
Myocytes
Enzymes
Hyaluronidase : 0.1%
Medium
Kreb's Ringers phosphate
Heart
Rat
Rat, adult, male, 150-200g
Grosso, D. , Frangakis, C. , Carlson, E. and Bressler, R.
Isolation and Characterization of Myocytes From the Adult Rat Heart ,
Prep Biochem
 7 (5) , 383 , 1977
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Medium
MEM CF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.