Tissue References Search Tool

Human

Gamero-Estevez, E. , Hensel, I. , Steinhauer, M. , Müllertz, O. , Savochkina, E. , Sektioglu, I. , Stoll, B. , Derakhshani, S. , Devriese, S. , Kim, K. and Resnik-Docampo, M.

Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine. ,
Bio Protoc
15 , e5157 , 2025

Cells

Intestinal organoids

Enzymes

Collagenase Type 4 : 0.2%

Deoxyribonuclease I : 0.015%

Medium

RPMI

Organoids

Human

Geest, A. , Jakobs, C. , Ljubikj, T. , Huffels, C. , Luna, M. , Sá, R. , Adolfs, Y. , Wit, M. , Rutten, D. , Kaal, M. , Zwartkruis, M. , Carcolé, M. , Groen, E. , Hol, E. , Basak, O. , Isaacs, A. , Westeneng, H. , Berg, L. , Veldink, J. , Schlegel, D. and Pasterkamp, R.

Molecular Pathology, Developmental Changes, and Synaptic Dysfunction in (pre-) Symptomatic Human C9ORF72-ALS/FTD Cerebral Organoids. , Acta Neuropathol Commun Vol. 12 , 152 , 2024

Cells

Cerebral organoids

Enzymes

Papain : 20 u/ml

Deoxyribonuclease I : 0.01%

Medium

DMEM/F12

Organoids

Human

Lisowski, P. , Lickfett, S. , Rybak-Wolf, A. , Menacho, C. and Le, S.

Mutant Huntingtin Impairs Neurodevelopment in Human Brain Organoids through CHCHD2-mediated Neurometabolic Failure. ,
Nat Commun
15 , 7027 , 2024

Cells

Brain organoids

Enzymes

Papain : 20 u/ml

Deoxyribonuclease I : 0.005%

Medium

EBSS

Organoids

Human

Marinucci, M. , Ercan, C. , Taha-Mehlitz, S. , Fourie, L. , Panebianco, F. , Bianco, G. , Gallon, J. , Staubli, S. , Soysal, S. , Zettl, A. , Rauthe, S. , Vosbeck, J. , Droeser, R. , Bolli, M. , Peterli, R. , Flüe, M. , Ng, C. , Kollmar, O. , Coto-Llerena, M. and Piscuoglio, S.

Standardizing Patient-Derived Organoid Generation Workflow to Avoid Microbial Contamination From Colorectal Cancer Tissues. , Front Oncol Vol. 11 , 781833 , 2021

Cells

Colorectal cancer organoids

Enzymes

Collagenase Type 4 : 0.25%

Deoxyribonuclease I : 0.01%

Hyaluronidase : 0.002%

Medium

DMEM/F-12

Organoids

Human

Schreurs, R. , Baumdick, M. , Drewniak, A. and Bunders, M.

Co-culture of Human Intestinal Organoids and Lamina Propria-derived CD4 T Cells. , STAR Protoc Vol. 2 , 100519 , 2021

Cells

Lamina propria lymphocytes

Enzymes

Collagenase Type 4 : 0.1%

Deoxyribonuclease I : 10 u/ml

Medium

DMEM/F-12

Organoids

Human

Human, embryonic

Nikolić, M. , Caritg, O. , Jeng, Q. , Johnson, J. , Sun, D. , Howell, K. , Brady, J. , Laresgoiti, U. , Allen, G. , Butler, R. , Zilbauer, M. , Giangreco, A. and Rawlins, E.

Human Embryonic Lung Epithelial Tips are Multipotent Progenitors that can be Expanded In vitro as Long-term Self-renewing Organoids. ,
Elife
6 , 2017

Cells

Lung organoids

Enzymes

Neutral Protease : 24 u/ml

Deoxyribonuclease I : 0.001%

Medium

DMEM/F12

Organoids

Mouse

Fujimura, T. , Enomoto, Y. , Katsura, H. , Ogawa, T. , Baba, S. , Ogata, A. , Yamaoka, A. , Shiroguchi, K. and Morimoto, M.

Identifying a Lung Stem Cell Subpopulation by Combining Single-Cell Morphometrics, Organoid Culture, and Transcriptomics. ,
Stem Cells
41 , 809-820 , 2023

Cells

Lung

Enzymes

Collagenase Type 1 : 450 u/ml

Elastase : 1 u/ml

Neutral Protease : 5 u/ml

Deoxyribonuclease I : 0.005%

Medium

DMEM/F12

Organoids

Mouse

Mouse, embryonic

Nikolić, M. , Caritg, O. , Jeng, Q. , Johnson, J. , Sun, D. , Howell, K. , Brady, J. , Laresgoiti, U. , Allen, G. , Butler, R. , Zilbauer, M. , Giangreco, A. and Rawlins, E.

Human Embryonic Lung Epithelial Tips are Multipotent Progenitors that can be Expanded In vitro as Long-term Self-renewing Organoids. ,
Elife
6 , 2017

Cells

Lung organoids

Enzymes

Neutral Protease : 24 u/ml

Deoxyribonuclease I : 0.001%

Medium

DMEM/F12

Organoids

Mouse

O'Rourke, K. , Ackerman, S. , Dow, L. and Lowe, S.

Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells. ,
Bio Protoc
6 , 2016