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Reproductive
Chicken

Chicken, male, 1 day

Huang, H. , Wang, M. , Hou, L. , Lin, X. , Pan, S. , Zheng, P. and Zhao, Q.
A Potential Mechanism Associated with Lead-induced Spermatogonia and Leydig Cell Toxicity and Mitigative Effect of Selenium in Chicken. , Ecotoxicol Environ Saf Vol. 209 , 111671 , 2021
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Trypsin : 0.1%
Medium
DMEM
Reproductive
Human
Human
Evans, J. and Salamonsen, L.
Decidualized Human Endometrial Stromal Cells are Sensors of Hormone Withdrawal in the Menstrual Inflammatory Cascade. ,
Biol Reprod
 90 , 14 , 2014
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Patel, J. , Seppanen, E. , Chong, M. , Yeo, J. , Teo, E. , Chan, J. , Fisk, N. and Khosrotehrani, K.
Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta. ,
Stem Cells Transl Med
 2 , 839-47 , 2013
Cells
Endothelial colony forming
Enzymes
Medium
HBSS
Reproductive
Human
Human
Patel, M. , Ghosh, M. , Fahey, J. and Wira, C.
Uterine Epithelial Cells Specifically Induce Interferon-Stimulated Genes in Response to Polyinosinic-Polycytidylic Acid Independently of Estradiol. ,
PLoS ONE
 7 , e35654 , 2012
Cells
Uterine epithelial
Enzymes
Pancreatin : 0.34%
Hyaluronidase : 0.01%
Collagenase : 0.16%
Medium
HBSS
Reproductive
Human
Human
Shalini, V. , Pratheep, S. , Muhammad, A. , Sharmili, V. , Elizabeth, G. and Rajesh, R.
Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta ,
Regenerative Research
 1 , 48 , 2012
Cells
Mesenchymal stem
Enzymes
Medium
DMEM/F12
Reproductive
Human
Human, female
Lockwood, C. , Arcuri, F. , Toti, P. , Felice, C. , Krikun, G. , Guller, S. , Buchwalder, L. and Schatz, F.
Tumor Necrosis Factor-Alpha and Interleukin-1 Beta Regulate Interleukin-8 Expression in Third Trimester Decidual Cells: Implications for the Genesis of Chorioamnionitis. ,
Am J Pathol
 169 , 1294-302 , 2006
Cells
Decidual
Enzymes
Collagenase : 0.25%
Deoxyribonuclease I : 6.25 u/ml
Medium
DMEM/F12
Reproductive
Human
Human
Spessotto, P. , Bulla, R. , Danussi, C. , Radillo, O. , Cervi, M. , Monami, G. , Bossi, F. , Tedesco, F. , Doliana, R. and Colombatti, A.
EMILIN1 Represents a Major Stromal Element Determining Human Trophoblast Invasion of the Uterine Wall. ,
J Cell Sci
 119 , 4574 , 2006
Cells
Extravillanous trophoblasts and decidual stromal
Enzymes
Trypsin : 0.1-0.25%
Deoxyribonuclease I : 0.02-0.05%
Medium
HBSS RPMI
Reproductive
Human
Human, female
Meter, R. , Wira, C. and Fahey, J.
Secretion of Monocyte Chemotactic Protein-1 by Human Uterine Epithelium Directs Monocyte Migration in Culture. ,
Fertil Steril
 84 , 191 , 2005
Cells
Uterine epithelial
Enzymes
Pancreatin : 0.34%
Hyaluronidase : 0.01%
Collagenase : 0.16%
Medium
HBSS
Reproductive
Human
Human
Wang Xin, Athayde Neil, Trudinger Brian.
Microvascular endothelial cell activation is present in the umbilical placental microcirculation in fetal placental vascular disease ,
Am J Obstet Gynecol
 190 , 596-601 , 2004
Cells
Endothelial placental
Enzymes
Medium
DMEM
Reproductive
Human
Human, female, 34-51 yr
Chan Rachel W S, Schwab Kjiana E, Gargett Caroline E.
Clonogenicity of human endometrial epithelial and stromal cells ,
Biol Reprod
 70 , 1738-50 , 2004
Cells
Endometrial epithelial and stromal cells
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Sun Kang, Myatt Leslie.
Enhancement of glucocorticoid-induced 11beta-hydroxysteroid dehydrogenase type 1 expression by proinflammatory cytokines in cultured human amnion fibroblasts ,
Endocrinology
 144 , 5568-77 , 2003
Cells
Amnion epithelial and fibroblast
Enzymes
Trypsin : 0.125%
Collagenase : 0.1%
Medium
PBS
Reproductive
Human
Human
Fahey John V, Wira Charles R.
Effect of menstrual status on antibacterial activity and secretory leukocyte protease inhibitor production by human uterine epithelial cells in culture ,
J Infect Dis
 185 , 1606-13 , 2002
Cells
Uterine epithelial cells
Enzymes
Pancreatin : 0.34%
Collagenase : 0.16%
Hyaluronidase : 0.16%
Medium
HBSS
Reproductive
Human
Human, female
Witz Craig A, Allsup Karen T, Montoya-Rodriguez Iris A, Vaughn Shelby L, Centonze Victoria E, Schenken Robert S.
Culture of menstrual endometrium with peritoneal explants and mesothelial monolayers confirms attachment to intact mesothelial cells ,
Hum Reprod
 17 , 2832-8 , 2002
Cells
Mesothelial
Enzymes
Medium
Eagle's MEM
Reproductive
Human
Human, female, 20-40 year
Gargett CE, Bucak K, Rogers PA.
Isolation, characterization and long-term culture of human myometrial microvascular endothelial cells ,
Hum Reprod
 15 , 293-301 , 2000
Cells
Microvascular endothelial cells
Enzymes
Medium
PBS
Reproductive
Human
Human
Friden BE, Runesson E, Hahlin M, Brannstrom M.
Evidence for nitric oxide acting as a luteolytic factor in the human corpus luteum ,
Mol Hum Reprod
 6 , 397-403 , 2000
Cells
Corpus luteum cells
Enzymes
Medium
PBS
Reproductive
Human
Human
Runesson E, Ivarsson K, Janson P, Brannstrom M.
Gonadotropin- and cytokine-regulated expression of the chemokine interleukin 8 in the human preovulatory follicle of the menstrual cycle ,
J Clin Endocrinol Metab
 85 , 4387-95 , 2000
Cells
Theca cells
Enzymes
Medium
PBS
Reproductive
Human
Human, female
Zhang J, Lathbury LJ, Salamonsen LA.
Expression of the chemokine eotaxin and its receptor, CCR3, in human endometrium ,
Biol Reprod
 62 , 404-11 , 2000
Cells
Endometrial epithelial cells
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human, females, 25-45 yr
Friden BE, Hagstrom H, Lindblom B, Sjoblom P, Wallin A, Brannstrom M, Hahlin M.
Cell characteristics and function of two enriched fraction of human luteal cells prolonged culture ,
Mol Hum Reprod
 5 , 714-9 , 1999
Cells
Luteal cells
Enzymes
Medium
PBS
Reproductive
Human
Human
Bradbury. , Sunn, K. , Crossley, M. , Bai, M. , Brown, E. , Delbridge, D. and Conigrave, A.
Expression of the Parathyroid Ca 2+ - Sensing Receptor in Cytotrophoblasts From Human Term Placenta ,
J Endocrinol
 156 , 425-430 , 1998
Cells
Placental
Enzymes
Medium
PSS
Reproductive
Human
Human
Zhang J, Nie G, Jian W, Woolley DE, Salamonsen LA.
Mast cell regulation of human endometrial matrix metalloproteinases: A mechanism underlying menstruation ,
Biol Reprod
 59 , 693-703 , 1998
Cells
Stromal endometrial
Enzymes
Medium
DMEM/Ham's F12
Reproductive
Human
Human
Egan, D. , Grzegorczyk, V. , Tricarico, K. , Rueter, A.H. , Olleman, W. and Marcotte, P.
Human Placental Chorionic Renin: Production, Purification and Characterization ,
Biochim Biophys Acta
 965 , 68 , 1988
Cells
Chorionic, placental
Enzymes
Medium
HBSS
Reproductive
Insect
Drosophila, embryonic
Salmand, P. Iche-Torres, M. and Perrin, L.
Tissue-Specific Cell Sorting from Drosophila Embryos: Application to Gene Expression Analysis. , Fly (Austin) Vol. 5 , 261
Cells
Cardiac differentiating
Enzymes
Collagenase Type 1 : 20 u/ml
Trypsin : 0.25%
Medium
Schneider
Reproductive
Monkey
Rhesus macaque
Xu, J, Lawson, MS, Yeoman, RR, Pau, KY, Barrett, SL, Zelinski, MB. and Stouffer, RL
Secondary Follicle Growth and Oocyte Maturation During Encapsulated Three-Dimensional Culture in Rhesus Monkeys: Effects of Gonadotrophins, Oxygen and Fetuin. ,
Hum Reprod
 26 , 1061-72 , 2011
Cells
Follicle
Enzymes
Medium
HEPES
Reproductive
Mouse
Mouse, adult
Lee, A, Rusch, J. , Lima, A. , Usmani, A. , Huang, N. , Lepamets, M. , Vigh-Conrad, K. , Worthington, R. and et al.
Rare Mutations in the Complement Regulatory Gene CSMD1 are Associated with Male and Female Infertility. ,
Nat Commun
 10 , 4626 , 2019
Cells
Testes
Enzymes
Medium
MEM
Reproductive
Mouse
Mouse, 1-7 day
Kang, H. , Chen, L. , Lichti-Kaiser, K. , Liao, G. , Gerrish, K. , Bortner, C. , Yao, Hu. , Eddy, E. and Jetten, A.
Transcription Factor GLIS3: A New and Critical Regulator of Postnatal Stages of Mouse Spermatogenesis. ,
Stem Cells
 34 , 2772-2783 , 2016
Cells
Testicular
Enzymes
Medium
HBSS
Reproductive
Mouse
Mouse, adult
Martin, L. and Seandel, M.
Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-Term Adult Mouse SSC Lines. ,
J Vis Exp
 72 , e50017 , 2013
Cells
Spermatogonial stem
Enzymes
Trypsin : 0.05%
Medium
DMEM
Reproductive
Mouse
Mouse, male
Getun, I. , Torres, B. and Bois, P.
Flow Cytometry Purification of Mouse Meiotic Cells. ,
J Vis Exp
 50 , 2602 , 2011
Cells
Testis, meiotic
Enzymes
Collagenase Type 1 : 120 u/ml
Trypsin : 0.1%
Medium
Gey's BSS
Reproductive
Mouse
Mouse
Chen, L. , Belton, R. and Nowak, R.
Basigin-Mediated Gene Expression Changes in Mouse Uterine Stromal Cells During Implantation. ,
Endocrinology
 150 , 966 , 2009
Cells
Uterine stomal
Enzymes
Medium
DMEM/F12
Reproductive
Mouse
Mouse, 6 day
Nalbandian Angele, Dettin Luis, Dym Martin, Ravindranath Neelakanta.
Expression of vascular endothelial growth factor receptors during male germ cell differentiation in the mouse ,
Biol Reprod
 69 , 985-94 , 2003
Cells
Sertoli
Enzymes
Medium
DMEM
Reproductive
Mouse
Mouse, 6-12 day
Eppig JJ, O'Brien MJ.
Development in vitro of mouse oocytes from primordial follicles ,
Biol Reprod
 54 , 197-207 , 1996
Cells
Oocyte-granulosa
Enzymes
Medium
Waymouth
Reproductive
Mouse
Mouse, male, 10-13 wk
Stalvcey, J. and Payne, A.
Luteinizing Hormone Receptors and Testosterone Production in Whole Testes and Purified Leydig Cells from the Mouse: Differences among Inbred Strains ,
Endocrinology
 112 , 1696 , 1983
Cells
Leydig
Testis
Enzymes
Medium
Medium 199 w/ BSA
Reproductive
Ovine
Ovine, 3-5 year
Letouzey, V. , Tan, K. , Deane, J. , Ulrich, D. , Gurung, S. , Ong, Y. and Gargett, C.
Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium. ,
PLoS ONE
 10 , e0127531 , 2015
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12
Reproductive
Porcine
Porcine, male, 8 day
Dirami G, Ravindranath N, Pursel V, Dym M.
Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM ,
Biol Reprod
 61 , 225-30 , 1999
Cells
Seminiferous epithelial cells
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.15%
Trypsin : 0.05%
Medium
DMEM/F12
Reproductive
Rabbit
Rabbit, New Zealand, 3 month
Kubota, H. , Wu, X. , Goodyear, S. , Avarbock, M. and Brinster, R.
Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties. ,
FASEB J
 25 , 2604 , 2011
Cells
Testicular germ
Enzymes
Medium
HBSS
Reproductive
Rabbit
Rabbit, New Zealand, 3 month
Kubota, H. , Wu, X. , Goodyear, S. , Avarbock, M. and Brinster, R.
Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties. ,
FASEB J
 25 , 2604 , 2011
Cells
Testicular germ
Enzymes
Medium
HBSS
Reproductive
Rabbit
Rabbit, New Zealand white, adult, female (nonpregnant),
3-4 kg
Phillippe, M. , Saunders, T. and Bangalore, S.
Alpha-1,Alpha-2,and Beta Adrenergic Signal Transduction in Cultured Uterine Myocytes ,
In Vitro Cell Dev Biol
 26 , 369 , 1990
Cells
Myocytes, uterine
Enzymes
Deoxyribonuclease I : 200 µg/ml
Medium
HBSS-HEPES buffer

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.