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Tissue References Search Tool

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Heart
Canine
Canine
Xi, Y. , Wu, G. , Ai, T. , Cheng, N. , Kalisnik, J. , Sun, J. , Abbasi, S. , Yang, D. , Fan, C. , Yuan, X. and Wang, S. et al.
Ionic Mechanisms Underlying the Effects of Vasoactive Intestinal Polypeptide on Canine Atrial Myocardium. ,
Circ Arrhythm Electrophysiol
 6 , 976-83 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 300 u/ml
Protease : 0.03%
Medium
Tyrode's solution
Heart
Canine
Canine, adult
Gavi, S. , Yin, D. , Shumay, E. , Wang, H. and Malbon, C.
Insulin-Like Growth Factor-I Provokes Functional Antagonism and Internalization of Beta1-Adrenergic Receptors. ,
Endocrinology
 148 , 2653-62 , 2007
Cells
Cardiomyocytes
Enzymes
Protease : 0.008%
Medium
M199
Heart
Chicken
Chicken, embryonic
Blech-Hermoni, Y. and Ladd, A.
Identification of Transcripts Regulated by CUG-BP, Elav-like Family Member 1 (CELF1) in Primary Embryonic Cardiomyocytes by RNA-seq. ,
Genom Data
 6 , 74-76 , 2015
Cells
Cardiomyocytes
Enzymes
Medium
HBSS
Heart
Human
Human
Voigt, N. , Zhou, X. and Dobrev, D.
Isolation of Human Atrial Myocytes for Simultaneous Measurements of Ca2+ Transients and Membrane Currents. ,
J Vis Exp
 , e50235 , 2013
Cells
Atrial myocytes
Enzymes
Collagenase Type 1 : 286 u/ml
Protease : 5 u/ml
Medium
see references
Heart
Human
Human
Voigt, N. , Makary, S. , Nattel, S. and Dobrev, D.
Voltage-Clamp-Based Methods for the Detection of Constitutively Active Acetylcholine-Gated I(K,ACh) Channels in the Diseased Heart. ,
Methods Enzymol
 484 , 653 , 2010
Cells
Atrial cardiomyocytes
Enzymes
Collagenase Type 1 : 286 u/ml
Protease : 5 u/ml
Medium
see reference
Heart
Mouse

Mouse, adult

Zhang, Z. , Zheng, W. , He, D. , Hu, Z. , Xie, Q. , Huang, M. , Li, W. and Huang, Z.
An Improved Procedure for Isolating Adult Mouse Cardiomyocytes for Epicardial Activation Mapping. ,
J Cell Mol Med
 25 , 11257-11263 , 2021
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 35-50 u/ml
Protease : 0.005%
Medium
Tyrode solution
Heart
Mouse
Mouse
Hu, D. , Yin, C. , Mohanta, S. , Weber, C. and Habenicht, A.
Prepartaion of Single Cell Suspensions from Mouse Aorta ,
Bio Protoc
 6 , 11 , 2016
Cells
Aortic
Enzymes
Collagenase Type 1 : 400 u/ml
Collagenase : 120 u/ml
Hyaluronidase : 60 u/ml
Medium
DPBS
Heart
Mouse
Mouse, adult
Santos Nascimento, D, Mosqueira, D, Sousa, L, Teixeira, M, Filipe, M, Resende, T, Araujo, A, Valente, M, Almeida, J, Martins, J, Santos, J, Barcia, R, Cruz, P, Cruz, H. and Pinto-d-O, P
Human Umbilical Cord Tissue-Derived Mesenchymal Stromal Cells Attenuate Remodeling after Myocardial Infarction by Proangiogenic, Antiapoptotic, and Endogenous Cell-Activation Mechanisms. ,
Stem Cell Res Ther
 5 , 5 , 2014
Cells
Cardiac Progenitor
Enzymes
Medium
MEM
Heart
Mouse
Mouse
Touchberry, C. , Green, T. , Tchikrizov, V. , Mannix, J. , Mao, T. , Carney, B. , Girgis, M. , Vincent, R. , Wetmore, L. , Dawn, B. , Bonewald, L. , Stubbs, J. and Wacker, M.
FGF23 is a Novel Regulator of Intracellular Calcium and Cardiac Contractility in Addition to Cardiac Hypertrophy. ,
Am J Physiol Endocrinol Metab
 304 , E863 , 2013
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 360 u/ml
Papain : 0.4 u/ml
Medium
L-15
Heart
Mouse
Mouse, adult, 9-13 week
Kabaeva, Z. , Zhao, M. and Michele, D.
Blebbistatin Extends Culture Life of Adult Mouse Cardiac Myocytes and Allows Efficient and Stable Transgene Expression. ,
Am J Physiol Heart Circ Physiol
 294 , H1667-74 , 2008
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 620 u/ml
Protease XIV : 0.104 u/ml
Medium
Myocyte buffer see reference
Heart
Mouse
Mouse, 6-8 week
Qian, Q. , Hunter, L. , Li, M. , Marin-Padilla, M. , Prakash, Y. , Somlo, S. , Harris, P. , Torres, V. and Sieck, G.
Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells ,
Hum Mol Genet
 12(15) , 1875 , 2003
Cells
Vascular smooth muscle
Enzymes
Papain : 10 u/ml
Elastase : .005%
Collagenase : 0.05%
Deoxyribonuclease I : 1000 u/ml
PDS kit : with modifications
Medium
EBSS
Heart
Mouse
Mouse, 1-6 day
Watzka SB, Lucien J, Shimada M, Edwards V, Yeger H, Hannigan G. and Coles JG.
Selection of viable cardiomyocytes for cell transplantation using three-dimensional tissue culture ,
Transplantation
 70 , 1310 , 2000
Cells
Cardiomyocytes
Enzymes
Medium
PBS
Heart
Mouse
Mouse, 6-9 or 21-41 day
Valenzuela, D. , Han, X. , Mende, U. , Fankhauser, C. , Mashimo, H. , Huang, P. , Pfeffer, J. , Neer, E. and Fishman, M.
G alpha(o) is Necessary for Muscarinic Regulation of Ca2+ Channels in Mouse Heart ,
Proc Natl Acad Sci U S A
 94(5) , 1727 , 1997
Cells
Ventricular and atrial myocytes
Enzymes
Collagenase Type 2 : 95 u/ml
Hyaluronidase : 172.5 u/ml
Trypsin : 0.002%
Medium
DMEM/Tyrodes
Heart
Rabbit
Rabbit, New Zealand, 2.3-2.9 kg
Farkas, A. , Acsai, K. , Nagy, N. , Toth, A. , Fulop, F. , Seprenyi, G. , Birinyi, P. , Nanasi, P. , Forster, T. , Csanady, M. , Papp, J. , Varrro, A. and Farkas, A.
Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart. ,
Br J Pharmacol
 154 , 93 , 2008
Cells
Cardiomyocytes
Enzymes
Hyaluronidase : see reference
Protease : see reference
Medium
Krebs-Henseleit
Heart
Rat
Rat, adult
Zhang, Y. , Wang, Y. , Yanni, J. , Qureshi, M. , Logantha, S. , Kassab, S. , Boyett, M. , Gardiner, N. , Sun, H. , Howarth, F. and Dobrzynski, H.
Electrical Conduction System Remodeling in Streptozotocin-Induced Diabetes Mellitus Rat Heart. ,
Front Physiol
 10 , 826 , 2019
Cells
Sinoatrial nodal
Enzymes
Collagenase Type 4 : 220 u/ml
Elastase : 1.4 u/ml
Protease : 0.45 u/ml
Medium
Tyrode solution
Heart
Rat
Rat, Wistar, 200g
Miller, L. , Greensmith, D. , Sankaranarayanan, R. , O'Neill, S. and Eisner D.
The Effect of 2,5-di-(tert-butyl)-1,4-benzohydroquinone (TBQ) on Intracellular Ca2+ Handling in Rat Ventricular Myocytes. ,
Cell Calcium
 58 , 208-14 , 2015
Cells
Ventricular myocytes
Enzymes
Collagenase Type 2 : 95 u/ml
Protease : 0.1 u/ml
Medium
HEPES
Heart
Rat
Rat, SD, 200-250 g
Farkas, A. , Acsai, K. , Nagy, N. , Toth, A. , Fulop, F. , Seprenyi, G. , Birinyi, P. , Nanasi, P. , Forster, T. , Csanady, M. , Papp, J. , Varrro, A. and Farkas, A.
Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart. ,
Br J Pharmacol
 154 , 93 , 2008
Cells
Cardiomyocytes
Enzymes
Hyaluronidase : see reference
Protease : see reference
Medium
Krebs-Henseleit
Heart
Rat
Rat, male, 250-300 g
Hunton DaciaL, Zou LuYun, Pang Yi, Marchase Richard B.
Adult rat cardiomyocytes exhibit capacitative calcium entry ,
Am J Physiol Heart Circ Physiol
 286 , H1124-32 , 2004
Cells
Cardiomyocytes
Enzymes
Protease : 0.01%
Medium
HBSS
Heart
Rat
Rat, SD, 175-200g
Tardif Annie, Julien Nathalie, Chiasson Jean-Louis, Coderre Lise.
Stimulation of glucose uptake by chronic vanadate pretreatment in cardiomyocytes requires PI 3-kinase and p38 MAPK activation ,
Am J Physiol/Endo
 284 , E1055-64 , 2003
Cells
Cardiomyocytes
Enzymes
Medium
Krebs-Ringer
Heart
Rat
Rat, male, 6-12 months old
Kitta, K. , Day, R. , Ikeda, T. and Suzuki, Y.
Hepatocyte Growth Factor Protects Cardiac Myocytes Against Oxidative Stress-Induced Apoptosis ,
Free Radic Biol Med
 31 (7) , 902 , 2001
Cells
Cardiac myocytes
Enzymes
Protease : 0.55 u/ml
Medium
Tyrode's solution

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.