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Tissue References Search Tool

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Skin
Mouse
Mouse, 2-3 day
Farina, G. , York, M. , Di Marzio, M. , Collins, C. , Meller, S. , Homey, B. , Rifkin, I. , Marshak-Rothstein, A. , Radstake, T. and Lafyatis, R.
Poly(I:C) Drives Type I IFN- and TGFβ-Mediated Inflammation and Dermal Fibrosis Simulating Altered Gene Expression in Systemic Sclerosis. ,
J Invest Dermatol
 130 , 2583-93 , 2010
Cells
Dermal fibroblasts
Enzymes
Trypsin : 0.025%
Medium
DME
Skin
Mouse
Mouse
Eidsmo, L. , Allan, R. , Caminschi, I. , Van Rooijen, N. , Heath, W. and Carbone, F.
Differential Migration of Epidermal and Dermal Dendritic Cells During Skin Infection. ,
J Immunol
 182 , 3165 , 2009
Cells
Dermal
Enzymes
Medium
HBSS
Skin
Mouse
Mouse, neonatal
Crigler Lauren, Kazhanie Amita, Yoon Tae-Jin, Zakhari Julia, Anders Joanna, Taylor Barbara, Virador VictoriaM.
Isolation of a mesenchymal cell population from murine dermis that contains progenitors of multiple cell lineages ,
FASEB J
 21 , 2050-63 , 2007
Cells
Dermal
Enzymes
Collagenase : 0.35%
Deoxyribonuclease I : see reference
Medium
PBS
Skin
Mouse
mouse
Baxter Ruth M, Crowell Thomas P, McCrann Margaret E, Frew Erica M, Gardner Humphrey.
Analysis of the tight skin (Tsk1/+) mouse as a model for testing antifibrotic agents ,
Lab Invest
 85 , 1199-209 , 2005
Cells
Dermal fibroblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Mouse
Mouse
Takanami-Ohnishi Yoko, Amano Shinya, Kimura Sadao, Asada Sachie, Utani Atsushi, Maruyama Masumi, Osada Hiroyuki, Tsunoda Hajime, Irukayama-Tomobe Yoko, Goto Katsutoshi, Karin Michael, Sudo Tatsuhiko, Kasuya Yoshitoshi.
Essential role of p38 mitogen-activated protein kinase in contact hypersensitivity ,
J Biol Chem
 277 , 37896-903 , 2002
Cells
Ear epidermal
Enzymes
Trypsin : 0.1%
Collagenase : 0.2%
Medium
PBS
Skin
Mouse
Mouse, 3-7 month
Bradshaw AD, Francki A, Motamed K, Howe C, Sage EH.
Primary mesenchymal cells isolated from SPARC-null mice exhibit altered morphology and rates of proliferation ,
Mol Biol Cell
 10 , 1569-79 , 1999
Cells
Fibroblasts, mesangial, smooth muscle
Enzymes
Trypsin : 0.25%
Collagenase : see reference
Medium
DMEM
Skin
Rabbit

Rabbit, Leporidae

Santos, F. , Carvalho, C. , Almeida, I. , Fagulha, T. , Rammos, F. , Barros, S. , Henriques, M. , Luís, T. and Duarte, M.
Simple Method for Establishing Primary Leporidae Skin Fibroblast Cultures. ,
Cells
 10 , 2021
Cells
Fibroblast
Enzymes
Trypsin : 0.25%
Medium
DMEM/HAMSF12
Skin
Rat
Rat, Wistar, 4-8 day
Sugihara, H. and Toda, S
Primary Tissue Intact and Dissociated Cell Culture , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 3A:2.1 , 1995
Cells
Dermal fibroblasts and keratinocytes
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Skin
Rat
Rat, SD, male, 40-60 days
Laurent, S. , Mednieks, M. and Rosenfield, R.
Growth of Sebaceous Cells in Monolayer Culture ,
In Vitro Cell Dev Biol
 28 , 83 , 1992
Cells
Sebaceous
Enzymes
Trypsin : 0.2%
Medium
DMEM with FBS
PBS
Skin
Rat
Rat, neonatal, 12-24 hr post partum
Acheson, A. , Barker, P. , Alderson, R. , Miller, F. and Murphy, R.
Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF ,
Neuron
 7 , 265 , 1991
Cells
Fibroblasts
Enzymes
Trypsin : 0.2%
Medium
HEPES buffered DMEM
Skin
Rat
Rat, albino, one day old, CFN
Vaughan, F. , Gray, R. and Bernstein, I.
Growth and Differentiation of Primary Rat Keratinocytes on Synthetic Membranes ,
In Vitro Cell Dev Biol
 22 (3) , 141 , 1986
Cells
Keratinocytes
Enzymes
Trypsin : 1%
Medium
EBSS
Spleen
Mouse
Mouse
Yi, T. and Cyster, J.
EBI2-Mediated Bridging Channel Positioning Supports Splenic Dendritic Cell Homeostasis and Particulate Antigen Capture. ,
Elife
 2 , e00757 , 2013
Cells
Splenocytes
Enzymes
Medium
PBS
Spleen
Mouse
Mouse
Shi, C. , Jia, T. , Mendez-Ferrer, S. , Hohl, T. , Serbina, N. , Lipuma, L. , Leiner, I. , Li, M. , Frenette, P. and Pamer, E.
Bone Marrow Mesenchymal Stem and Progenitor Cells Induce Monocyte Emigration in Response to Circulating Toll-Like Receptor Ligands. ,
Immunity
 34 , 590 , 2011
Cells
Spleen, bone marrow endothelial
Enzymes
Medium
PBS
Spleen
Mouse
Mouse
Abe, K. , Nguyen, K. , Fine, S. , Mo, J. , Shen, C. , Shenouda, S. , Corr, M. , Jung, S. , Lee, J. , Eckmann, L. and Raz, E.
Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells. ,
Proc Natl Acad Sci U S A
 104 , 17022 , 2007
Cells
Dentritic
Enzymes
Collagenase : 300 u/ml
Medium
RPMI 1640
Spleen
Mouse
Mouse
Kang, H. , Liu, M. and Datta, S.
Low-dose Peptide Tolerance Therapy of Lupus Generates Plasmacytoid Dendritic Cells That Cause Expansion of Autoantigen-Specific Regulatory T Cells and Contraction of Inflammatory Th17 Cells ,
J Immunol
 178 , 7849-58 , 2007
Cells
Splenic dendritic
Enzymes
Medium
DMEM
Spleen
Mouse
Mouse
Mueller, S. , Matloubian, M. , Clemens, D, Sharpe, A. , Freeman, G. , Gangappa, S. , Larsen, C. and Ahmed, R.
Viral Targeting of Fibroblastic Reticular Cells Contributes to Immunosuppression and Persistence During Chronic Infection. ,
Proc Natl Acad Sci U S A
 104 , 15430 , 2007
Cells
Splenic
Enzymes
Medium
RPMI
Spleen
Mouse
Mouse
Schiavoni, F, Mattei, F, Sestili, P, Borghi, P, Venditti, M, Morse, H, Belardelli, F. and Gabrieli, L
ICSBP is Essential for the Development of Mouse Type I Interferon-producing Cells and for the Generation and Activation of CD8a+ Dendritic Cells ,
J Exp Med
 196 , 1415 , 2002
Cells
Dentritic
Enzymes
Medium
RPMI 1640
Spleen
Mouse
Mouse, 4-6 week
McLellan, A. , Kapp, M. , Eggert, A. , Linden, C. , Bommhardt, U. , Brocker, E. , Kammerer, U. and Kampgen, E.
Anatomic Location and T-cell Stimulatory Functions of Mouse Dendritic Cell Subsets Defined by CD4 and CD8 Expression ,
Blood
 99 , 2084-93 , 2002
Cells
Dentritic
Enzymes
Medium
RPMI-1640
Spleen
Mouse
Mouse
Stagg, AJ, Burke, F, Hill, S. and Knight, S.
Isolation of Mouse Spleen Dendritic Cells. ,
Methods Mol Med
 64 , 9-22 , 2001
Cells
Dentritic cells
Enzymes
Medium
HEPES/RPMI 1640
Stem
Canine
Canine
Park, S. , Seo, M. , Kim, H. and Kang, K.
Isolation and Characterization of Canine Amniotic Membrane-Derived Multipotent Stem Cells. ,
PLoS ONE
 7 , e44693 , 2012
Cells
Amniotic membrane MSC
Enzymes
Trypsin : 0.25%
Medium
LG-DMEM
Stem
Human
Human
de Witte, S. , Lambert, E. , Merino, A. , Strini, T. , Douben, H. , O'Flynn, L. , Elliman, S. , de Klein, A. , Newsome, P. , Baan, C. and Hoogduijn, M.
Aging of Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stromal Cells During Expansion. ,
Cytotherapy
 19 , 798-807 , 2017
Cells
Umbilical cord mesenchymal stromal
Enzymes
Medium
MEM
Stem
Human
Human
Kusuma, G. , Menicanin, D. , Gronthos, S. , Manuelpillai, U. , Abumaree, M. , Pertile, M. , Brennecke, S. and Kalionis, B.
Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua. ,
PLoS ONE
 10 , e0141246 , 2015
Cells
Decidua mesenchymal stem
Enzymes
Medium
PBS
Stem
Human
Human
Hang, H. , Yu, Y. , Wu, N. , Huang, Q. , Xia, Q. and Bian, J.
Induction of Highly Functional Hepatocytes from Human Umbilical Cord Mesenchymal Stem Cells by HNF4α Transduction. ,
PLoS ONE
 9 , e104133 , 2014
Cells
Umbilical mesenchymal stem
Enzymes
Trypsin : 0.25%
Medium
DMEM
Stem
Human
Human
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Type 2 Alveolar Cells are Stem Cells in Adult Lung ,
J Clin Invest
 123 , 3025 , 2013
Cells
Alveolar epithelial
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 0.25%
Elastase : 10 u/ml
Medium
Bronchial Epithelial Growth Medium
Stem
Human
Human
Dobbin, Z. and Landen, C.
Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications. ,
Curr Protoc Pharmacol
 63 , Unit 14.28. , 2013
Cells
Cancer stem
Enzymes
Trypsin : 0.25%
Collagenase : 0.05%
Hyaluronidase : 0.005%
Medium
RPMI
Stem
Human
Human
Han, Y. , Tao, R. , Sun, T. , Chai, J. , Xu, G. and Liu, J.
Optimization of Human Umbilical Cord Mesenchymal Stem Cell Isolation and Culture Methods. ,
Cytotechnology
 65 , 819 , 2013
Cells
Umbilical cord mesenchymal stem
Enzymes
Collagenase : 0.1-0.2%
Trypsin : 0.1%
Medium
DMEM
Stem
Human
Human, adult
Nagel, S. , Rohr, F. , Weber, C. , Kier, J. , Siemers, F. , Kruse, C. , Danner, S. , Brandenburger, M. and Matthiessen, A.
Multipotent Nestin-Positive Stem Cells Reside in the Stroma of Human Eccrine and Apocrine Sweat Glands and can be Propagated Robustly In Vitro. ,
PLoS ONE
 8 , e78365 , 2013
Cells
Epidermal stem cells
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 2.5%
Medium
DMEM
Stem
Human
Human
Patel, J. , Seppanen, E. , Chong, M. , Yeo, J. , Teo, E. , Chan, J. , Fisk, N. and Khosrotehrani, K.
Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta. ,
Stem Cells Transl Med
 2 , 839-47 , 2013
Cells
Endothelial colony forming
Enzymes
Medium
HBSS
Stem
Human
Human
Welte, Y. , Davies, C. , Schafer, R. and Regenbrecht, C.
Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma. ,
J Vis Exp
 73 , e50200 , 2013
Cells
Melanoma
Enzymes
Medium
PBS
Stem
Human
Human
Salehinejad, P. , Alitheen, N. , Ali, A. , Omar, A. , Mohit, M. , Janzamin, E. , Samani, F. , Torshizi, Z. and Nematollahi-Mahani, S.
Comparison of Different Methods for the Isolation of Mesenchymal Stem Cells from Human Umbilical Cord Wharton's Jelly. ,
In Vitro Cell Dev Biol Anim
 48 , 75 , 2012
Cells
Wharton jelly mesenchymal stem
Enzymes
Trypsin : 0.25%
Medium
DMEM
Stem
Human
Human
Shalini, V. , Pratheep, S. , Muhammad, A. , Sharmili, V. , Elizabeth, G. and Rajesh, R.
Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta ,
Regenerative Research
 1 , 48 , 2012
Cells
Mesenchymal stem
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Nazarov, I. , Lee, J. , Soupene, E. , Etemad, S. , Knapik, D. , Green, W. , Bashkirova, E. , Fang, X. , Matthay, M. , Kuypers, F. and Serikov, V.
Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential. ,
Stem Cells Transl Med
 1 , 359-72 , 2012
Cells
Chorionic mesenchymal
Enzymes
Neutral Protease : 2.5 u/ml
Trypsin : 0.25%
Medium
MEM
Stem
Human
Human
Vellasamy, S. , Sandrasaigaran, P. , Vidyadaran, S. , George, E. and Ramasamy, R.
Isolation and Characterisation of Mesenchymal Stem cells Derived from Human Placenta Tissue. ,
World J Stem Cells
 4 , 53-61 , 2012
Cells
Placental mesenchymal stem
Enzymes
Medium
DMEM
Stem
Human
Human, fetal
Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.
Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
 70 , e4181 , 2012
Cells
Liver hematopoietic
Enzymes
Medium
RPMI
Stem
Human
Human, male 40-60 years
Blasi, A. , Martino, C. , Balducci, L. , Saldarelli, M. , Soleti, A. , Navone, S. , Canzi, L. , Cristini, S. , Invernici, G. , Parati, E. and Alessandri, G.
Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential ,
Vasc Cell
 3 , 5 , 2011
Cells
Adipose derived stem cells
Enzymes
Collagenase : 0.25%
Medium
PBS
Stem
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.