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Tissue References Search Tool

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Adipose/Fat
Human
Human, male 40-60 years
Blasi, A. , Martino, C. , Balducci, L. , Saldarelli, M. , Soleti, A. , Navone, S. , Canzi, L. , Cristini, S. , Invernici, G. , Parati, E. and Alessandri, G.
Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential ,
Vasc Cell
 3 , 5 , 2011
Cells
Adipose derived stem cells
Enzymes
Collagenase : 0.25%
Medium
PBS
Adrenal
Bovine
Bovine
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Bovine
Bovine
Hahm, S. , Chen, Y. , Vinson, C. and Eiden, L.
A Calcium-Initiated Signaling Pathway Propagated Through Calcineurin and cAMP Response Element-Binding Protein Activates Proenkephalin Gene Transcription after Depolarization. ,
Mol Pharmacol
 64 , 1503 , 2003
Cells
Chromaffin cells
Enzymes
Collagenase : 0.1%
Medium
DMEM
Adrenal
Bovine
Bovine
Zhu, J. , Li, W. , Toews, M. and Hexum, T.
Neuropeptide Y Inhibits Forskolin-Stimulated Adenylate Cyclase in Bovine Adrenal Chromaffin Cells via a Pertussis Toxin-Sensitive Process ,
J Pharmacol Exp Ther
 263 (3) , 1479 , 1992
Cells
Chromaffin
Enzymes
Medium
HEPES
Adrenal
Bovine
Bovine
Higgins, L. and Berg, D.
Immunological Identification of a Nicotinic Acetylcholine Receptor on Bovine Chromaffin Cells ,
J Neurosci
 7 (6) , 1792 , 1987
Cells
Chromaffin
Enzymes
Medium
Locke's solution
Adrenal
Bovine
Bovine
Wilson, S.P. and Viveros, O.H.
Primary Culture of Adrenal Medullary Chromaffin Cells in a Chemically Defined Medium ,
Exp Cell Res
 133 , 159 , 1981
Cells
Chromaffin
Enzymes
Deoxyribonuclease I : 15 µg/ml
Medium
Medium A
(see reference)
Adrenal
Human
Human
Caroccia, B. , Fassina, A. , Seccia, T. , Recarti, C. , Petrelli, L. , Belloni, A. , Pelizzo, M. and Rossi, G.
Isolation of Human Adrenocortical Aldosterone-Producing Cells by a Novel Immunomagnetic Beads Method. ,
Endocrinology
 151 , 1375 , 2010
Cells
Adrenocortical
Enzymes
Medium
Krebs Ringer
Brain
Human
Human
Scherpelz, K. , Lu, J.,Tycko, R. and Meredith, S.
Preparation of Amyloid Fibrils Seeded from Brain and Meninges ,
Methods Mol Biol
 1345 , 299 , 2016
Cells
Amyloid Fibrils
Enzymes
Medium
50mM Tris
Brain
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Brain
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Brain
Human
Human
Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P.
Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. , Neuro Oncol Vol. 11 , 109 , 2009
Cells
Tumor
Enzymes
Medium
see reference
Brain
Human
Human
Klegeris Andis, McGeer PatrickL.
Chymotrypsin-like proteases contribute to human monocytic THP-1 cell as well as human microglial neurotoxicity ,
Glia
 51 , 56-64 , 2005
Cells
Microglia
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Brain
Human
Human
Roher, A.E. , Palmer, K.C. , Chau, V. and Ball, M.J.
Isolation and Chemical Characterization of Alzheimer's Disease Paired Helical Filament Cytoskeletons:Differentiation from Amyloid Plaque Core Protein ,
J Cell Biol
 107 , 2703 , 1988
Cells
Neuronal
Enzymes
Deoxyribonuclease I : 10 µg/ml
Medium
Tris-HCl, 50 mM, CaCl2, 2 mM
Cartilage
Human
Human, adult
Liagre B, Vergne-Salle P, Corbiere C, Charissoux JL. and Beneytout JL.
Diosgenin, a Plant Steroid, Induces Apoptosis in Human Rheumatoid Arthritis Synoviocytes with Cyclooxygenase-2 Overexpression ,
Arthritis Res Ther
 6(4) , R373 , 2004
Cells
Synovial
Enzymes
Medium
DMEM
Cartilage
Human
Human
Dunham B P, Koch R J.
Basic fibroblast growth factor and insulinlike growth factor I support the growth of human septal chondrocytes in a serum-free environment ,
Arch Otolaryngol Head Neck Surg
 124 , 1325-30 , 1998
Cells
Septal chondrocytes
Enzymes
Medium
DMEM/F12
Colon
Human
Human
Moser, A. , Spindelboeck, W. , Strohmaier, H. , Enzinger, C. , Gattringer, T. , Fuchs, S. , Fazekas, F. , Gorkiewicz, G. , Wurm, P. , Hogenauer, C. and Khalil, M.
Mucosal Biopsy Shows Immunologic Changes of the Colon in Patients with Early MS ,
Neurol Neuroimmunol Neuroinflamm
 4 , e362 , 2017
Cells
Lamina propria mononuclear
Enzymes
Medium
HBSS
Colon
Human
Human
Rollo, B. , Zhang, D. , Stamp, L. , Menheniott, T. , Stathopoulos, L. , Denham, M. , Dottori, M. , King, S. , Hutson, J. and Newgreen, D.
Enteric Neural Cells from Hirschsprung Disease Patients Form Ganglia in Autologous Aneuronal Colon ,
Cell Mol Gastroenterol Hepatol
 2 , 92 , 2016
Cells
Colon enteric neural
Enzymes
Medium
Ham's F-12
Colon
Human
Human
Zhou, J. , Belov, L. , Huang, P. , Shin, J. , Solomon, M. , Chapuis, P. , Bokey, L. , Chan, C. , Clarke, C. , Clarke, S. and Christopherson, R.
Surface Antigen Profiling of Colorectal Cancer Using Antibody Microarrays With Fluorescence Multiplexing. ,
J Immunol Methods
 355 , 40 , 2010
Cells
Colorectal cancer
Enzymes
Medium
HBSS
Colon
Human
Human
Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F.
Phenotypic characterization of human colorectal cancer stem cells ,
Proc Natl Acad Sci U S A
 104 , 10158-63 , 2007
Cells
Cancer stem cell
Enzymes
Collagenase Type 3 : 200 u/ml
Deoxyribonuclease I : 100 u/ml
Medium
RPMI-1640
Colon
Human
Human
Fukushima, K. and Fiocchi, C.
Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. ,
Am J Physiol Gastrointest Liver Physiol
 286 , G804-13 , 2004
Cells
Colonic epithelial
Enzymes
Medium
RPMI 1640
Colon
Human
Human
Hisamatsu, T. , Watanabe, M. , Ogata, H. , Ezaki, T. , Hozawa, S. , Ishii, H. , Kanai, T. and Hibi, T.
Interferon-Inducible Gene Family 1-8U Expression in Colitis-Associated Colon Cancer and Severely Inflamed Mucosa in Ulcerative Colitis. ,
Cancer Res
 59 , 5927-31 , 1999
Cells
Epithelial and mucosal lymphocytes
Enzymes
CLSPA : 0.02%
Medium
RPMI 1640
Epithelial
Human
Human
Fukushima, K. and Fiocchi, C.
Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. ,
Am J Physiol Gastrointest Liver Physiol
 286 , G804-13 , 2004
Cells
Colonic epithelial
Enzymes
Medium
RPMI 1640
Epithelial
Human
Human
Widdicombe, J.H. , Coleman, D.L. , Finkbeiner, W.E. and Tuet, I.K.
Electrical Properties of Monolayers Cultured From Cells of Human Tracheal Mucosa ,
J Appl Physiol
 58 , 1729 , 1985
Cells
Epithelial
Enzymes
Medium
HEPES with 5.9mM Glucose,5mM DTT
Eye
Bovine
Bovine
Terlizzi, V. , Kolibabka, M. , Burgess, J. , Hammes, H. and Harmsen, M.
The Pericytic Phenotype of Adipose Tissue-Derived Stromal Cells Is Promoted by NOTCH2. ,
Stem Cells
 36 , 240-251 , 2018
Cells
Retinal endothelial
Enzymes
Pronase : 0.02%
Medium
DMEM
Intestine
Human
Human
Trapecar, M. , Khan, S. , Roan, N. , Chen, T. , Telwatte, S. , Deswal, M. , Pao, M. , Somsouk, M. , Deeks, S. , Hunt, P. , Yukl, S. and Sanjabi, S.
An Optimized and Validated Method for Isolation and Characterization of Lymphocytes from HIV+ Human Gut Biopsies. ,
AIDS Res Hum Retroviruses
 33 , S31-S39 , 2017
Cells
Lymphocytes
Enzymes
CLSPA : 0.05%
Deoxyribonuclease I : 0.00075%
Medium
RPMI-1640
Intestine
Human
Human
Stallmach A, Schaffer F, Hoffmann S, Weber S, Muller-Molaian I, Schneider T, Kohne G, Ecker KW, Feifel G, Zeitz M.
Increased state of activation of CD4 positive T cells and elevated interferon gamma production in pouchitis ,
Gut
 43 , 499-505 , 1998
Cells
Mucosal mononuclear cells
Enzymes
Medium
RPMI
Liver
Human

Human

Kucykowicz, S. , Amin, O. , Burton, A. , Swadling, L. , Schmidt, N. , Zakeri, N. , Davies, J. , Aidoo-Micah, G. , Stegmann, K. , Easom, N. , Jeffery-Smith, A. , Maini, M. and Pallett, L.
Isolation of Human Intrahepatic Leukocytes for Phenotypic and Functional Characterization by Flow Cytometry. ,
Star Protocols
 3 , 101356 , 2022
Cells
Intrahepatic leukocytes
Enzymes
Medium
RPMI-1640
Liver
Human
Human, fetal
Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.
Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
 70 , e4181 , 2012
Cells
Liver hematopoietic
Enzymes
Medium
RPMI
Lung
Human

Human

Konishi, S. , Tata, A. and Tata, P.
Defined Conditions for Long-term Expansion of Murine and Human Alveolar Epithelial Stem Cells in Three-dimensional Cultures. , STAR Protoc Vol. 3 , 101447 , 2022
Cells
Alveolar epithelial stem
Enzymes
Collagenase Type 1 : 450 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Human

Human

Youk, J. , Kim, T. , Evans, K. , Jeong, Y. , Hur, Y. , Hong, S. , Kim, J. , Yi, K. , Kim, S. , Na, K. , Bleazard, T. , Kim, H. , Fellows, M. , Mahbubani, K. , Saeb-Parsy, K. , Kim, S. , Kim, Y. , Koh, G. , Choi, B. , Ju, Y. and Lee, J.
Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2. , Cell Stem Cell Vol. 27 , 905-919.e10 , 2020
Cells
Aveolar
Enzymes
Medium
DMEM
Lung
Human
Human
Quatromoni, J. , Singhal, S. , Bhojnagarwala, P. , Hancock, W. , Albelda, S. and Eruslanov, E.
An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells ,
J Leukoc Biol
 97 , 201 , 2015
Cells
Lung tumor
Enzymes
Collagenase Type 1 : 45-60 u/ml
Collagenase Type 2 : 15-20 u/ml
Collagenase Type 4 : 45-60 u/ml
Elastase : 0.002%
Medium
DMEM/F12
Lung
Human
Human
Fujino, N. , Kubo, H. , Suzuki, T. , Ota, C. , Hegab, A. , He, M. , Suzuki, S. , Suzuki, T. , Yamada, M. , Kondo, T. , Kato, H. and Yamaya, M.
Isolation of Alveolar Epithelial Type II Progenitor Cells from Adult Human Lungs. ,
Lab Invest
 91 , 363 , 2011
Cells
Lung
Enzymes
Medium
DMEM
Mammary
Bovine
Bovine, lactating Holstein
Miranda, S. , Wang, Y. , Purdie, N. , Osborne, V. , Coomber, B. and Cant, J.
Selenomethionine Stimulates Expression of Glutathione Peroxidase 1 and 3 and Growth of Bovine Mammary Epithelial Cells in Primary Culture. ,
J Dairy Sci
 92 , 2670 , 2009
Cells
Mammary epithelial
Enzymes
Collagenase Type 3 : 400 u/ml
Hyaluronidase : 100 u/ml
Medium
HBSS
Mammary
Bovine
Bovine, heifer, 200kg
Weber M. , Purup S. , Vestergaard M. , Ellis S. , Scndergard-Andersen J. , Akers R. and Sejrsen K.
Contribution of Insulin-like Growth Factor (IGF)-I and IGF-binding Protein-3 to Mitogenic Activity in Bovine Mammary Extracts and Serum ,
J Endocrinol
 161 , 365 , 1999
Cells
Mammary epithelial
Enzymes
Medium
M-199
Mammary
Bovine
Bovine
Baumrucker, C. , Deemer, K. , Walsh, R. , Riss, T. and Akers, R.
Primary Culture of Bovine Mammary Acini on a Collagen Matrix ,
Tissue Cell
 20 (4) , 541 , 1988
Cells
Epithelial
Enzymes
Medium
HBSS/Medium 199
Miscellaneous
Human
Human
Patino WillmarD, Kang Ju-Gyeong, Matoba Satoaki, Mian OmarY, Gochuico BernadetteR, Hwang PaulM.
Atherosclerotic plaque macrophage transcriptional regulators are expressed in blood and modulated by tristetraprolin ,
Circ Res
 98 , 1282-9 , 2006
Cells
Carotid artery plaque macrophage
Enzymes
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.