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Tissue References Search Tool

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Organoids
Human

Human

Schreurs, R. , Baumdick, M. , Drewniak, A. and Bunders, M.
Co-culture of Human Intestinal Organoids and Lamina Propria-derived CD4 T Cells. , STAR Protoc Vol. 2 , 100519 , 2021
Cells
Lamina propria lymphocytes
Enzymes
Medium
DMEM/F-12
Organoids
Human

Human, embryonic

Nikolić, M. , Caritg, O. , Jeng, Q. , Johnson, J. , Sun, D. , Howell, K. , Brady, J. , Laresgoiti, U. , Allen, G. , Butler, R. , Zilbauer, M. , Giangreco, A. and Rawlins, E.
Human Embryonic Lung Epithelial Tips are Multipotent Progenitors that can be Expanded In vitro as Long-term Self-renewing Organoids. ,
Elife
 6 , 2017
Cells
Lung organoids
Enzymes
Medium
DMEM/F12
Pancreas
Human
Human
Brissova, M. , Haliyur, R. , Saunders, D. , Shrestha, S. , Dai, C. , Blodgett, D. , Bottino, R. , Campbell-Thompson, M. , Aramandla, R. , Poffenberger, G. , Lindner, J. , Pan, F. , von Herrath, M. , Greiner, D. and Shultz, L.
α Cell Function and Gene Expression Are Compromised in Type 1 Diabetes. ,
Cell Rep
 22 , 2667-2676 , 2018
Cells
Islets
Enzymes
Collagenase Type 1 : 1,600u/isolation
Neutral Protease : 200 u/isolation
Deoxyribonuclease I : 12,000 u/isolation
Medium
RPMI
Pancreas
Human
Human
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Reproductive
Human
Human
Evans, J. and Salamonsen, L.
Decidualized Human Endometrial Stromal Cells are Sensors of Hormone Withdrawal in the Menstrual Inflammatory Cascade. ,
Biol Reprod
 90 , 14 , 2014
Cells
Endometrial stromal
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Patel, J. , Seppanen, E. , Chong, M. , Yeo, J. , Teo, E. , Chan, J. , Fisk, N. and Khosrotehrani, K.
Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta. ,
Stem Cells Transl Med
 2 , 839-47 , 2013
Cells
Endothelial colony forming
Enzymes
Medium
HBSS
Reproductive
Human
Human
Shalini, V. , Pratheep, S. , Muhammad, A. , Sharmili, V. , Elizabeth, G. and Rajesh, R.
Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta ,
Regenerative Research
 1 , 48 , 2012
Cells
Mesenchymal stem
Enzymes
Medium
DMEM/F12
Reproductive
Human
Human, female
Lockwood, C. , Arcuri, F. , Toti, P. , Felice, C. , Krikun, G. , Guller, S. , Buchwalder, L. and Schatz, F.
Tumor Necrosis Factor-Alpha and Interleukin-1 Beta Regulate Interleukin-8 Expression in Third Trimester Decidual Cells: Implications for the Genesis of Chorioamnionitis. ,
Am J Pathol
 169 , 1294-302 , 2006
Cells
Decidual
Enzymes
Collagenase : 0.25%
Deoxyribonuclease I : 6.25 u/ml
Medium
DMEM/F12
Reproductive
Human
Human
Spessotto, P. , Bulla, R. , Danussi, C. , Radillo, O. , Cervi, M. , Monami, G. , Bossi, F. , Tedesco, F. , Doliana, R. and Colombatti, A.
EMILIN1 Represents a Major Stromal Element Determining Human Trophoblast Invasion of the Uterine Wall. ,
J Cell Sci
 119 , 4574 , 2006
Cells
Extravillanous trophoblasts and decidual stromal
Enzymes
Trypsin : 0.1-0.25%
Deoxyribonuclease I : 0.02-0.05%
Medium
HBSS RPMI
Reproductive
Human
Human
Wang Xin, Athayde Neil, Trudinger Brian.
Microvascular endothelial cell activation is present in the umbilical placental microcirculation in fetal placental vascular disease ,
Am J Obstet Gynecol
 190 , 596-601 , 2004
Cells
Endothelial placental
Enzymes
Medium
DMEM
Reproductive
Human
Human, female, 34-51 yr
Chan Rachel W S, Schwab Kjiana E, Gargett Caroline E.
Clonogenicity of human endometrial epithelial and stromal cells ,
Biol Reprod
 70 , 1738-50 , 2004
Cells
Endometrial epithelial and stromal cells
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Sun Kang, Myatt Leslie.
Enhancement of glucocorticoid-induced 11beta-hydroxysteroid dehydrogenase type 1 expression by proinflammatory cytokines in cultured human amnion fibroblasts ,
Endocrinology
 144 , 5568-77 , 2003
Cells
Amnion epithelial and fibroblast
Enzymes
Trypsin : 0.125%
Collagenase : 0.1%
Medium
PBS
Reproductive
Human
Human, female
Witz Craig A, Allsup Karen T, Montoya-Rodriguez Iris A, Vaughn Shelby L, Centonze Victoria E, Schenken Robert S.
Culture of menstrual endometrium with peritoneal explants and mesothelial monolayers confirms attachment to intact mesothelial cells ,
Hum Reprod
 17 , 2832-8 , 2002
Cells
Mesothelial
Enzymes
Medium
Eagle's MEM
Reproductive
Human
Human, female, 20-40 year
Gargett CE, Bucak K, Rogers PA.
Isolation, characterization and long-term culture of human myometrial microvascular endothelial cells ,
Hum Reprod
 15 , 293-301 , 2000
Cells
Microvascular endothelial cells
Enzymes
Medium
PBS
Reproductive
Human
Human
Friden BE, Runesson E, Hahlin M, Brannstrom M.
Evidence for nitric oxide acting as a luteolytic factor in the human corpus luteum ,
Mol Hum Reprod
 6 , 397-403 , 2000
Cells
Corpus luteum cells
Enzymes
Medium
PBS
Reproductive
Human
Human
Runesson E, Ivarsson K, Janson P, Brannstrom M.
Gonadotropin- and cytokine-regulated expression of the chemokine interleukin 8 in the human preovulatory follicle of the menstrual cycle ,
J Clin Endocrinol Metab
 85 , 4387-95 , 2000
Cells
Theca cells
Enzymes
Medium
PBS
Reproductive
Human
Human, female
Zhang J, Lathbury LJ, Salamonsen LA.
Expression of the chemokine eotaxin and its receptor, CCR3, in human endometrium ,
Biol Reprod
 62 , 404-11 , 2000
Cells
Endometrial epithelial cells
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human, females, 25-45 yr
Friden BE, Hagstrom H, Lindblom B, Sjoblom P, Wallin A, Brannstrom M, Hahlin M.
Cell characteristics and function of two enriched fraction of human luteal cells prolonged culture ,
Mol Hum Reprod
 5 , 714-9 , 1999
Cells
Luteal cells
Enzymes
Medium
PBS
Reproductive
Human
Human
Bradbury. , Sunn, K. , Crossley, M. , Bai, M. , Brown, E. , Delbridge, D. and Conigrave, A.
Expression of the Parathyroid Ca 2+ - Sensing Receptor in Cytotrophoblasts From Human Term Placenta ,
J Endocrinol
 156 , 425-430 , 1998
Cells
Placental
Enzymes
Medium
PSS
Reproductive
Human
Human
Zhang J, Nie G, Jian W, Woolley DE, Salamonsen LA.
Mast cell regulation of human endometrial matrix metalloproteinases: A mechanism underlying menstruation ,
Biol Reprod
 59 , 693-703 , 1998
Cells
Stromal endometrial
Enzymes
Medium
DMEM/Ham's F12
Reproductive
Human
Human
Egan, D. , Grzegorczyk, V. , Tricarico, K. , Rueter, A.H. , Olleman, W. and Marcotte, P.
Human Placental Chorionic Renin: Production, Purification and Characterization ,
Biochim Biophys Acta
 965 , 68 , 1988
Cells
Chorionic, placental
Enzymes
Medium
HBSS
Skin
Human
Human
Botting, Ra. , Bertram, K. , Baharlou, H. , Sandgren, K. , Fletcher, J. , Rhodes, J. , Rana, H. , Plasto, T. , Wang, X. , Lim, J. , Barnouti, L. , Kohout, M. , Papadopoulos, T. , Merten, S. , Olbourne, N. and Cunningham, A.
Phenotypic and Functional Consequences of Different Isolation Protocols on Skin Mononuclear Phagocytes. ,
J Leukoc Biol
 101 , 1393-1403 , 2017
Cells
Mononuclear phagocytes
Enzymes
Neutral Protease : 1 u/ml
Collagenase : 200 u/ml
Trypsin : 0.5%
Medium
RPMI-1640
Skin
Human
Human
Cheuk, S. , Wiken, M. , Blomqvist, L. , Nylen, S. , Talme, T. and Stahle, M.and Eidsmo, L.
Epidermal Th22 and Tc17 Cells Form a Localized Disease Memory in Clinically Healed Psoriasis. ,
J Immunol
 192 , 3111 , 2014
Cells
Skin
Enzymes
Medium
RPMI 1640
Skin
Human
Human
Cerny, D. , Haniffa, M. , Shin, A. , Bigliardi, P. , Tan, B. , Lee, B. , Poidinger, M. , Tan, E. , Ginhoux, F. and Fink, K.
Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. ,
PLoS Pathog
 10 , e1004548 , 2014
Cells
Skin
Enzymes
Medium
RPMI
Skin
Human
Human, adult
Alitalo K, Kuismanen E, Myllyla R, Kiistala U, Asko-Seljavaara S, Vaheri A.
Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells ,
J Cell Biol
 94 , 497-505 , 1982
Cells
Epidermal keratinocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.2%
Medium
Eagle's MEM
Stem
Human
Human
de Witte, S. , Lambert, E. , Merino, A. , Strini, T. , Douben, H. , O'Flynn, L. , Elliman, S. , de Klein, A. , Newsome, P. , Baan, C. and Hoogduijn, M.
Aging of Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stromal Cells During Expansion. ,
Cytotherapy
 19 , 798-807 , 2017
Cells
Umbilical cord mesenchymal stromal
Enzymes
Medium
MEM
Stem
Human
Human
Kusuma, G. , Menicanin, D. , Gronthos, S. , Manuelpillai, U. , Abumaree, M. , Pertile, M. , Brennecke, S. and Kalionis, B.
Ectopic Bone Formation by Mesenchymal Stem Cells Derived from Human Term Placenta and the Decidua. ,
PLoS ONE
 10 , e0141246 , 2015
Cells
Decidua mesenchymal stem
Enzymes
Medium
PBS
Stem
Human
Human
Patel, J. , Seppanen, E. , Chong, M. , Yeo, J. , Teo, E. , Chan, J. , Fisk, N. and Khosrotehrani, K.
Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta. ,
Stem Cells Transl Med
 2 , 839-47 , 2013
Cells
Endothelial colony forming
Enzymes
Medium
HBSS
Stem
Human
Human
Welte, Y. , Davies, C. , Schafer, R. and Regenbrecht, C.
Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma. ,
J Vis Exp
 73 , e50200 , 2013
Cells
Melanoma
Enzymes
Medium
PBS
Stem
Human
Human
Shalini, V. , Pratheep, S. , Muhammad, A. , Sharmili, V. , Elizabeth, G. and Rajesh, R.
Generation and Characterisation of Human Mesenchymal Stem Cells Derived From Umbilical Cord and Placenta ,
Regenerative Research
 1 , 48 , 2012
Cells
Mesenchymal stem
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Vellasamy, S. , Sandrasaigaran, P. , Vidyadaran, S. , George, E. and Ramasamy, R.
Isolation and Characterisation of Mesenchymal Stem cells Derived from Human Placenta Tissue. ,
World J Stem Cells
 4 , 53-61 , 2012
Cells
Placental mesenchymal stem
Enzymes
Medium
DMEM
Stem
Human
Human, fetal
Vatakis, D. , Bristol, G. , Kim, S. , Levin, B. , Liu, W. , Radu, C. , Kitchen, S. and Zack, J.
Using the BLT Humanized Mouse as a Stem Cell Based Gene Therapy Tumor Model. ,
J Vis Exp
 70 , e4181 , 2012
Cells
Liver hematopoietic
Enzymes
Medium
RPMI
Stem
Human
Human, male 40-60 years
Blasi, A. , Martino, C. , Balducci, L. , Saldarelli, M. , Soleti, A. , Navone, S. , Canzi, L. , Cristini, S. , Invernici, G. , Parati, E. and Alessandri, G.
Dermal Fibroblasts Display Similar Phenotypic and Differentiation Capacity to Fat-Derived Mesenchymal Stem Cells, but Differ in Anti-Inflammatory and Angiogenic Potential ,
Vasc Cell
 3 , 5 , 2011
Cells
Adipose derived stem cells
Enzymes
Collagenase : 0.25%
Medium
PBS
Stem
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P.
Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. , Neuro Oncol Vol. 11 , 109 , 2009
Cells
Tumor
Enzymes
Medium
see reference
Stem
Human
Human, male
Kossack, N. , Meneses, J. , Shefi, S. , Nguyen, H. , Chavez, S. , Nicholas, C. , Gromoll, J. , Turek, P. and Reijo-Pera, R.
Isolation and Characterization of Pluripotent Human Spermatogonial Stem Cell-Derived Cells. ,
Stem Cells
 , 2008
Cells
Spermatogonial stem cells
Enzymes
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.