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Tissue References Search Tool

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Adrenal
Bovine
Bovine
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Bovine
Bovine
Hahm, S. , Chen, Y. , Vinson, C. and Eiden, L.
A Calcium-Initiated Signaling Pathway Propagated Through Calcineurin and cAMP Response Element-Binding Protein Activates Proenkephalin Gene Transcription after Depolarization. ,
Mol Pharmacol
 64 , 1503 , 2003
Cells
Chromaffin cells
Enzymes
Collagenase : 0.1%
Medium
DMEM
Adrenal
Bovine
Bovine
Zhu, J. , Li, W. , Toews, M. and Hexum, T.
Neuropeptide Y Inhibits Forskolin-Stimulated Adenylate Cyclase in Bovine Adrenal Chromaffin Cells via a Pertussis Toxin-Sensitive Process ,
J Pharmacol Exp Ther
 263 (3) , 1479 , 1992
Cells
Chromaffin
Enzymes
Medium
HEPES
Adrenal
Bovine
Bovine
Higgins, L. and Berg, D.
Immunological Identification of a Nicotinic Acetylcholine Receptor on Bovine Chromaffin Cells ,
J Neurosci
 7 (6) , 1792 , 1987
Cells
Chromaffin
Enzymes
Medium
Locke's solution
Adrenal
Bovine
Bovine
Wilson, S.P. and Viveros, O.H.
Primary Culture of Adrenal Medullary Chromaffin Cells in a Chemically Defined Medium ,
Exp Cell Res
 133 , 159 , 1981
Cells
Chromaffin
Enzymes
Deoxyribonuclease I : 15 µg/ml
Medium
Medium A
(see reference)
Adrenal
Human
Human
Caroccia, B. , Fassina, A. , Seccia, T. , Recarti, C. , Petrelli, L. , Belloni, A. , Pelizzo, M. and Rossi, G.
Isolation of Human Adrenocortical Aldosterone-Producing Cells by a Novel Immunomagnetic Beads Method. ,
Endocrinology
 151 , 1375 , 2010
Cells
Adrenocortical
Enzymes
Medium
Krebs Ringer
Adrenal
Mouse
Mouse
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Ovine
Ovine, adult
Keating, D. , Rychkov, G. , Adams, M. , Holgert, H. , McMillen, I.C. and Roberts, M.
Opioid Receptor Stimulation Suppresses the Adrenal Medulla Hypoxic Response in Sheep by Actions on Ca(2+) and K(+) Channels. ,
J Physiol
 555 , 489 , 2004
Cells
Chromaffin cells
Enzymes
Medium
Locke's solution
Adrenal
Ovine
Ovine, 3 year
Canny B J, O'Farrell K A, Clarke I J, Tilbrook A J.
The influence of sex and gonadectomy on the hypothalamo-pituitary-adrenal axis of the sheep ,
J Endocrinol
 162 , 215-25 , 1999
Cells
Anterior pituitary
Enzymes
Trypsin : 2.5%
Medium
DMEM
Adrenal
Rat
Rat
Dominguez, N. , Rodri­guez, M. , Machado, J. and Borges, R.
Preparation and Culture of Adrenal Chromaffin Cells. ,
Methods Mol Biol
 846 , 223 , 2012
Cells
Chromaffin
Enzymes
Collagenase Type 1 : 0.1-0.2%
Deoxyribonuclease I : 0.003-0.015%
Hyaluronidase : 0.015%
Medium
DMEM/F12
Adrenal
Rat
Rat
Gilabert, J, Montalvo, G. and Artalejo A.
Rat Chromaffin Cells Primary Cultures: Standardization and Quality Assessment for Single-Cell Assays ,
Nat Protoc
 , 294 , 2006
Cells
Chromaffin
Enzymes
Medium
HBSS
Adrenal
Rat
Rat, SD
Gilabert, J.
Necessary Conditions to Maintain Rat Adrenal Chromaffin Cells in Primary Culture , Cell Biology of the Chromaffin Cell , Borges, R. and Gandia, L. , Instituto Teofilo Hernando , 2004
Cells
Chromaffin cells
Enzymes
Medium
HBSS
Adrenal
Rat
Rat, Wistar, newborn
Zhang L, Castell A, Avila E, Drucker-Colín R, Escobar A.
Immunocytochemical, ultrastructural and neurochemical evidences on synaptogenesis and dopamine release of rat chromaffin cells co-cultured with striatal neurons ,
J Neuropathol Exp Neurol
 59 , 170-4 , 2000
Cells
Chromaffin cells
Enzymes
Medium
DMEM
Adrenal
Rat
Rat, Fischer, male, 10-16 weeks
Roskelley, C.D. and Auersperg, N.
Density Separation of Rat Adrenocortical Cells: Morphology, Steroidogenesis, and P-450scc Expression in Primary Culture ,
In Vitro Cell Dev Biol
 26 , 493 , 1990
Cells
Adrenocortical
Enzymes
Medium
BSS
Adrenal
Rat
Rat, Fischer, male, 1-10 months
Leonard, R.K. , Auersperg, N. and Parkes, C.O.
Ascorbic Acid Accumulation by Cultured Rat Adrenocortical Cells ,
In Vitro
 19 , 46 , 1983
Cells
Adrenocortical
Enzymes
Medium
BSS
Adrenal
Rat
Rat, SD, male, 400-450 g
Li, C.H. , Ng, T.B. and Cheng, C.H.K.
Melanotropins: Aldosterone- and Corticosterone-Stimulating Activity in Isolated Rat Adrenal Cells ,
Int J Pept Protein Res
 19 , 361 , 1982
Cells
Decapular
Capsular
Glomerulosa
Enzymes
Medium
Medium 199
Adrenal
Rat
Rat , SD, female, 200 g
Braley, L. , Williams, G. and Bradwin, G.
The Effect of Unit Gravity Sedimentation on Adrenal Steroidogenesis by Isolated Rat Glomerulosa and Fasciculata Cells ,
Endocrinology
 106 (1) , 50 , 1980
Cells
Glomerulosa
Enzymes
Medium
Kreb's
Muscle
Human
Human, female
Richardson, M. , Taylor, D. , Casey, M. , MacDonald, P. and Stull, J.
Biochemical Markers of Contraction in Human Myometrial Smooth Muscle Cells in Culture ,
In Vitro Cell Dev Biol
 23 , 21 , 1987
Cells
Smooth muscle
Myometrial
Enzymes
Deoxyribonuclease I : 0.015% and 0.007%
Medium
HBSS
Muscle
Human
Human, female
Casey, M. , MacDonald, P. , Mitchell, M. and Snyder, J.
Maintenance and Characterization of Human Myometrial Smooth Muscle Cells in Monolayer Culture ,
In Vitro
 20 , 396 , 1984
Cells
Smooth muscle
Myometrial
Enzymes
Medium
HBSS
Muscle
Ovine
Sheep, adult and neonatal
Driska S. , Laudadio R. , Wolfson M. and Shaffer T.
A Method for Isolating Adult and Neonatal Airway Smooth Muscle Cells and Measuring Shortening Velocity ,
J Appl Physiol
 86(1) , 427 , 1999
Cells
Tracheal smooth muscle cells
Enzymes
Papain : 0.2%
Medium
MOPS-PSS
Muscle
Porcine
Porcine, postnatal
Miersch, C. , Stange, K. and Rontgen, M.
Effects of Trypsinization and of a Combined Trypsin, Collagenase, and DNase Digestion on Liberation and in Vitro Function of Satellite Cells Isolated from Juvenile Porcine Muscles. ,
In Vitro Cell Dev Biol Anim
 54 , 406-412 , 2018
Cells
Muscle satellite
Enzymes
Medium
Eagle MEM
Muscle
Porcine
Porcine, male, 25-40 kg
Korzick, D. , Laughlin, M. and Bowles, D.
Alterations in PKC signaling underlie enhanced myogenic tone in exercise-trained porcine coronary resistance arteries ,
J Appl Physiol
 96 , 1425-32 , 2004
Cells
Coronary myocytes
Enzymes
Medium
low calcium physiological saline
Muscle
Porcine
Porcine, adult, 35-45 kg
Wamhoff BR, Dixon JL, Sturek M.
Atorvastatin treatment prevents alterations in coronary smooth muscle nuclear Ca2+ signaling in diabetic dyslipidemia ,
J Vasc Res
 39 , 208 , 2002
Cells
Arterial smooth muscle
Enzymes
Collagenase Type 2 : 294 u/ml
Elastase : 6.5 u/ml
Deoxyribonuclease I : 0.4 mg/ml
Medium
MEM
Muscle
Rat
Rat
Weber, S. , Gratopp, A. , Akanbi, S. , Rheinlaender, C. , Sallmon, H. , Barikbin, P. and Koehne, P.
Isolation and Culture of Fibroblasts, Vascular Smooth Muscle, and Endothelial Cells From the Fetal Rat Ductus Arteriosus ,
Pediatr Res
 70 , 236 , 2011
Cells
Vascular smooth muscle
Enzymes
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.