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Tissue References Search Tool

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Kidney
Canine
Dog, mongrel, 20 Kg
Yau, C. , Rao, L. and Silverman, M.
Sugar Uptake Into a Primary Culture of Dog Kidney Proximal Tubular Cells ,
Can J Physiol Pharmacol
 63 , 417 , 1985
Cells
Proximal tubular
Enzymes
Medium
(see reference)
Kidney
Mouse

Mouse

Li, Z. , Zimmerman, K. , Cherakara, S. , Chumley, P. , Collawn, J. , Wang, J. , Haycraft, C. , Song, C. , Chacana, T. , Andersen, R. , Croyle, M. , Aloria, E. , Hombal, R. , Thomas, I. , Chweih, H. , Simanyi, K. , George, J. , Parant, J. , Mrug, M. and Yoder, B.
A Kidney Resident Macrophage Subset is a Candidate Biomarker for Renal Cystic Disease in Preclinical Models. , Dis Model Mech Vol. 16 , 2023
Cells
Macrophages
Enzymes
Medium
RPMI 1640
Kidney
Mouse
Mouse
Yuan, Y. , Li, L. , Zhu, L. , Liu, F. , Tang, X. , Liao, G. , Liu, J. , Cheng, J. , Chen, Y. and Lu, Y.
Mesenchymal Stem Cells Elicit Macrophages into M2 Phenotype Via Improving Transcription Factor EB-Mediated Autophagy to Alleviate Diabetic Nephropathy. ,
Stem Cells
 38 , 639-652 , 2020
Cells
Mactrophages
Enzymes
Medium
DMEM
Kidney
Mouse
Mouse
Adam, M. , Potter, A. and Potter, S.
Psychrophilic Proteases Dramatically Reduce Single-Cell RNA-Seq Artifacts: A Molecular Atlas of Kidney Development. ,
Development
 144 , 3625-3632 , 2017
Cells
Kidney
Enzymes
Deoxyribonuclease I : 125 u/ml
Protease, Bacillus Licheniformis : 1%
Medium
DPBS
Kidney
Mouse
Mouse
Sedrakyan, S. , Da Sacco, S. , Milanesi, A. , Shiri, L. , Petrosyan, A. , Varimezova, R. , Warburton, D. , Lemley, K. , De Filippo, R. and Perin, L.
Injection of Amniotic Fluid Stem Cells Delays Progression of Renal Fibrosis. ,
J Am Soc Nephrol
 23 , 661-73 , 2012
Cells
Glomeruli
Enzymes
Medium
DMEM
Kidney
Mouse
Mouse, embryonic
Brown, A. , Blank, U. , Adams, D. , Karolak, M. , Fetting, J. , Hill, B. and Oxburgh, L.
Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney. ,
J Vis Exp
 50 , e2555 , 2011
Cells
Kidney
Enzymes
Medium
HBSS
Kidney
Mouse
Mouse, naive SJL (H-2S)
Haverty, T. , Kelly, C. , Hines, W. , Amenta, P. , Watanabe, M. , Harper, R. , Kefalides, N. and Neilson, E.
Characterization of a Renal Tubular Epithelial Cell Line Which Secretes the Autologous Target Antigen of Autoimmune Experimental Interstitial Nephritis ,
J Cell Biol
 107 , 1359 , 1988
Cells
Proximal tubular epithelial
Enzymes
Deoxyribonuclease I : 15 µg/ml
Medium
RPMI 1640
Kidney
Rabbit
Rabbit, New Zealand White, female
Schafer, J. , Watkins, M. , Li, L. , Herter, P. , Haxelmans, S. and Schlatter, E.
A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments ,
Am J Physiol
 273 , F650 , 1997
Cells
Nephron segments
Enzymes
Medium
Eagle's MEM
Kidney
Rabbit
Rabbit, New Zealand white, female, 2-3 Kg
Rodeheaver, D. , Aleo, M. and Schnellmann, R.
Differences in Enzymatic and Mechanical Isolated Rabbit Renal Proximal Tubules: Comparison in Long-Term Incubation ,
In Vitro Cell Dev Biol
 26 , 898 , 1990
Cells
Renal proximal tubules
Enzymes
Medium
Modified DME-F12
Kidney
Rat
Rat, SD, male
Schafer, J. , Watkins, M. , Li, L. , Herter, P. , Haxelmans, S. and Schlatter, E.
A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments ,
Am J Physiol
 273 , F650 , 1997
Cells
Nephron segments
Enzymes
Medium
Eagle's MEM
Lung
Human

Human

Konishi, S. , Tata, A. and Tata, P.
Defined Conditions for Long-term Expansion of Murine and Human Alveolar Epithelial Stem Cells in Three-dimensional Cultures. , STAR Protoc Vol. 3 , 101447 , 2022
Cells
Alveolar epithelial stem
Enzymes
Collagenase Type 1 : 450 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Human

Human

Youk, J. , Kim, T. , Evans, K. , Jeong, Y. , Hur, Y. , Hong, S. , Kim, J. , Yi, K. , Kim, S. , Na, K. , Bleazard, T. , Kim, H. , Fellows, M. , Mahbubani, K. , Saeb-Parsy, K. , Kim, S. , Kim, Y. , Koh, G. , Choi, B. , Ju, Y. and Lee, J.
Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2. , Cell Stem Cell Vol. 27 , 905-919.e10 , 2020
Cells
Aveolar
Enzymes
Medium
DMEM
Lung
Human
Human
Quatromoni, J. , Singhal, S. , Bhojnagarwala, P. , Hancock, W. , Albelda, S. and Eruslanov, E.
An Optimized Dissagregation Method for Human Lung Tumors That Perserves the Phenotype and Function of the Immune Cells ,
J Leukoc Biol
 97 , 201 , 2015
Cells
Lung tumor
Enzymes
Collagenase Type 1 : 45-60 u/ml
Collagenase Type 2 : 15-20 u/ml
Collagenase Type 4 : 45-60 u/ml
Elastase : 0.002%
Medium
DMEM/F12
Lung
Human
Human
Fujino, N. , Kubo, H. , Suzuki, T. , Ota, C. , Hegab, A. , He, M. , Suzuki, S. , Suzuki, T. , Yamada, M. , Kondo, T. , Kato, H. and Yamaya, M.
Isolation of Alveolar Epithelial Type II Progenitor Cells from Adult Human Lungs. ,
Lab Invest
 91 , 363 , 2011
Cells
Lung
Enzymes
Medium
DMEM
Lung
Mouse

Mouse

Horn, K. , Fulte, S. , Yang, M. , Lorenz, B. and Clark, S.
Neutrophil Responsiveness to IL-10 Impairs Clearance of Streptococcus pneumoniae from the Lungs. ,
J Leukoc Biol
 , 2023
Cells
Lung
Enzymes
Medium
PBS
Lung
Mouse

Mouse

Konishi, S. , Tata, A. and Tata, P.
Defined Conditions for Long-term Expansion of Murine and Human Alveolar Epithelial Stem Cells in Three-dimensional Cultures. , STAR Protoc Vol. 3 , 101447 , 2022
Cells
Alveolar epithelial stem
Enzymes
Collagenase Type 1 : 450 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Mouse
Mouse
Qiu, Y. , Xu, Y. , Wang, M. , Miao, M. , Zhou, H. , Xu, J. , Kong, J. , Zheng, D. , Li, R. , Zhang, R. , Guo, Y. , Li, X. , Cui, J. , Qin, C. and Zhou , X.
Flavivirus Induces and Antagonizes Antiviral RNA Interference in Both Mammals and Mosquitoes. ,
Sci. Adv.
 6 , 7989 , 2020
Cells
Fibroblasts
Enzymes
Medium
HBSS
Lung
Mouse
Mouse, 6 week
Lewis, K. , Hall, J. , Kiyotake, E. , Christensen, T. , Balasubramaniam, V. and Anseth, K.
Epithelial-Mesenchymal Crosstalk Influences Cellular Behavior in a 3D Alveolus-Fibroblast Model System. ,
Biomaterials
 155 , 124 , 2018
Cells
Alveolar epithelial
Enzymes
Medium
DMEM/F-12
Lung
Mouse
Mouse
Sun, F. , Xiao, G. and Qu, Z.
Isolation of Murine Alveolar Type II Epithelial Cells. ,
Bio-protocol
 7 , 2017
Cells
Alveolar epithelial
Enzymes
Medium
DMEM
Lung
Mouse
Mouse, 8-12 week
Singer, B. , Mock, J. , D'Alessio, F. , Aggarwal, N. , Mandke, P. , Johnston, L. and Damarla, M.
Flow-Cytometric Method for Simultaneous Analysis of Mouse Lung Epithelial, Endothelial, and Hematopoietic Lineage Cells. ,
Am J Physiol Lung Cell Mol Physiol
 310 , L796-801 , 2016
Cells
Epithelial, Endothelial
Enzymes
Medium
PBS
Lung
Mouse
Mouse
Yu, Y. , O'Koren, E. , Holten, D. , Kan, M. , Kojin, D. , Nelson, E. , Que, L. and Gunn, M.
A Protocol for the Comprehensive Flow Cytometric Analysis of Immune Cells in Normal and Inflammed Murine Non-Lymphoid Tissues ,
PLoS ONE
 10 , 1371 , 2016
Cells
Lung
Enzymes
Medium
PBS
Lung
Mouse
Mouse
Braza, F. , Dirou, S. , Forest, V. , Sauzeau, V. , Hassoun, D. , Chesne, J. , Cheminant-Muller, M. , Sagan, C. , Magnan, A. and Lemarchand, P.
Mesenchymal Stem Cells Induce Suppressive Macrophages Through Phagocytosis in a Mouse Model of Asthma. ,
Stem Cells
 34 , 1836-45 , 2016
Cells
Lung
Enzymes
Medium
PBS
Lung
Mouse
Mouse
Mohapatra, A. , Van Dyken, S. , Schneider, C. , Nussbaum, J. , Liang, H. and Locksley, R.
Group 2 Innate Lymphoid Cells Utilize the IRF4-IL-9 Module to Coordinate Epithelial Cell Maintenance of Lung Homeostasis. ,
Mucosal Immunol
 , 2015
Cells
Alveolar
Enzymes
Collagenase Type 1 : 300 u/ml
Elastase : 4 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 200 u/ml
Medium
HBSS
Lung
Mouse
Mouse, embryonic
Desai, T. , Brownfield, D. and Krasnow, M.
Alveolar Progenitor and Stem Cells in Lung Development, Renewal and Cancer. ,
Nature
 507 , 190-4 , 2014
Cells
Avelolar progenitor
Enzymes
Elastase : 3 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Mouse
Mouse
Kim, H. , Lee, H. , Chang, Y. , Pichavant, M. , Shore, S. , Fitzgerald, K. , Ikawura, Y. , Israel, E. , Bolger, K. , Faul, J. , DeKruyff, R. and Umetsu, D.
IL-17 Producing Innate Lymphoid Cells and the NLRP3 Inflammasone Facilitate Obescity-associated Airway Hyperractivity ,
Nat Med
 20 , 54 , 2014
Cells
Pulmonary
Enzymes
Medium
see reference
Lung
Mouse
Mouse
Misharin, A. , Morales-Nebreda, L. , Mutlu, G. , Budinger, G. and Perlman, H.
Flow Cytometric Analysis of Macrophages and Dendritic Cell Subsets in the Mouse Lung. ,
Am J Respir Cell Mol Biol
 49 , 503-10 , 2013
Cells
Lung
Enzymes
Medium
HBSS
Lung
Mouse
Mouse
Vaughan, A. , Halbert, C. , Wootton, S. and Miller, A.
Lung Cancer in Mice Induced by the Jaagsiekte Sheep Retrovirus Envelope Protein is not Maintained by Rare Cancer Stem Cells, but Tumorigenicity does Correlate with Wnt Pathway Activation. ,
Mol Cancer Res
 10 , 86 , 2012
Cells
Lung tumor
Enzymes
Neutral Protease : 50 u/ml
Collagenase : 400 u/ml
Medium
DMEM
Lung
Mouse
Mouse
Hardy, C. , LeMasurier, J. , Belz, G. , Scalzo-Inguanti, K. , Yao, J. , Xiang, S. , Kanellakis, P. , Bobik, A. , Strickland, D. , Rolland, J. , O'Hehir, R. and Plebanski, M.
Inert 50-nm Polystyrene Nanoparticles That Modify Pulmonary Dendritic Cell Function and Inhibit Allergic Airway Inflammation. ,
J Immunol
 188 , 1431 , 2012
Cells
Lung
Enzymes
Medium
RPMI 1640
Lung
Mouse
Mouse, 8-12 week
Driscoll, B. , Kikuchi, A. , Lau, A. , Lee, J. , Reddy, R. , Jesudason, E. , Kim, C. and Warburton, D.
Isolation and Characterization of Distal Lung Progenitor Cells. ,
Methods Mol Biol
 879 , 109 , 2012
Cells
Lung progenitor
Enzymes
Medium
DMEM
Lung
Mouse
Mouse
Rock, J, Barkauskas, C, Cronc, M, Xue, Y, Harris, J, Liang, J, Noble, P. and Hogan, B.
Multiple Stromal Populations Contribute to Pulmonary Fibrosis Without Evidence for Epithelial to Mesenchymal Transition. ,
Proc Natl Acad Sci U S A
 108 , E1475 , 2011
Cells
Lung
Enzymes
Collagenase Type 1 : 450 u/ml
Elastase : 4 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Mouse
Mouse
Lancelin, W. and Guerrero-Plata, A.
Isolation of Mouse Lung Dendritic Cells. ,
J Vis Exp
 57 , e3563 , 2011
Cells
Dendritic
Enzymes
Medium
HBSS
Lung
Mouse
Mouse, female, 6-12 week
Breslow, R. , Rao, J. , Xing, W. , Hong, D. , Barrett, N. and Katz, H.
Inhibition of Th2 Adaptive Immune Responses and Pulmonary Inflammation by Leukocyte Ig-Like Receptor B4 on Dendritic Cells. ,
J Immunol
 184 , 1003 , 2010
Cells
Lung mononuclear
Enzymes
Medium
HBSS
Lung
Mouse
Mouse
Ferreira, C. , Antunes, F. , Leonard, V. , Welstead, G. , Richardson, C. and Cattaneo, R.
Measles Virus Infection of Alveolar Macrophages and Dendritic Cells Precedes Spread to Lymphatic Organs in Transgenic Mice Expressing Human Signaling Lymphocytic Activation Molecule (SLAM, CD150). ,
J Virol
 84 , 3033 , 2010
Cells
Lung
Enzymes
Medium
RPMI 1640
Lung
Mouse
Mouse, 6-7 week
Jungblut, M. , Oeltze, K. , Zehnter, I. , Hasselmann, D. and Bosio, A.
Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue Using the GentleMACS Dissociator. ,
J Vis Exp
 29 , 2009
Cells
Leukocytes and endothelial
Enzymes
Collagenase : 0.2%
Deoxyribonuclease I : 40-80 u/ml
Medium
PBS
Lung
Mouse
Mouse
Zhao, J. , Zhao, J. , Van Rooijen, N. and Perlman, S.
Evasion by Stealth: Inefficient Immune Activation Underlies Poor T Cell Response and Severe Disease in SARS-CoV-Infected Mice. ,
PLoS Pathog
 5 , e1000636 , 2009
Cells
Lung
Enzymes
Medium
HBSS
Lung
Mouse
Mouse, embryo, day 18
Trotter, A. , Kipp, M. , Schrader, R. and Beyer, C.
Combined Application of 17Beta-Estradiol and Progesterone Enhance Vascular Endothelial Growth Factor and Surfactant Protein Expression in Cultured Embryonic Lung Cells of Mice. ,
Int J Pediatr
 2009 , 170491 , 2009
Cells
Alveolar and fibroblast
Enzymes
Trypsin : 2.5%
Collagenase Type 1 : 1250 u/ml
Medium
MEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.