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Tissue References Search Tool

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Colon
Human
Human
Moser, A. , Spindelboeck, W. , Strohmaier, H. , Enzinger, C. , Gattringer, T. , Fuchs, S. , Fazekas, F. , Gorkiewicz, G. , Wurm, P. , Hogenauer, C. and Khalil, M.
Mucosal Biopsy Shows Immunologic Changes of the Colon in Patients with Early MS ,
Neurol Neuroimmunol Neuroinflamm
 4 , e362 , 2017
Cells
Lamina propria mononuclear
Enzymes
Medium
HBSS
Colon
Human
Human
Rollo, B. , Zhang, D. , Stamp, L. , Menheniott, T. , Stathopoulos, L. , Denham, M. , Dottori, M. , King, S. , Hutson, J. and Newgreen, D.
Enteric Neural Cells from Hirschsprung Disease Patients Form Ganglia in Autologous Aneuronal Colon ,
Cell Mol Gastroenterol Hepatol
 2 , 92 , 2016
Cells
Colon enteric neural
Enzymes
Medium
Ham's F-12
Colon
Human
Human
Zhou, J. , Belov, L. , Huang, P. , Shin, J. , Solomon, M. , Chapuis, P. , Bokey, L. , Chan, C. , Clarke, C. , Clarke, S. and Christopherson, R.
Surface Antigen Profiling of Colorectal Cancer Using Antibody Microarrays With Fluorescence Multiplexing. ,
J Immunol Methods
 355 , 40 , 2010
Cells
Colorectal cancer
Enzymes
Medium
HBSS
Colon
Human
Human
Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F.
Phenotypic characterization of human colorectal cancer stem cells ,
Proc Natl Acad Sci U S A
 104 , 10158-63 , 2007
Cells
Cancer stem cell
Enzymes
Collagenase Type 3 : 200 u/ml
Deoxyribonuclease I : 100 u/ml
Medium
RPMI-1640
Colon
Human
Human
Fukushima, K. and Fiocchi, C.
Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. ,
Am J Physiol Gastrointest Liver Physiol
 286 , G804-13 , 2004
Cells
Colonic epithelial
Enzymes
Medium
RPMI 1640
Colon
Human
Human
Hisamatsu, T. , Watanabe, M. , Ogata, H. , Ezaki, T. , Hozawa, S. , Ishii, H. , Kanai, T. and Hibi, T.
Interferon-Inducible Gene Family 1-8U Expression in Colitis-Associated Colon Cancer and Severely Inflamed Mucosa in Ulcerative Colitis. ,
Cancer Res
 59 , 5927-31 , 1999
Cells
Epithelial and mucosal lymphocytes
Enzymes
CLSPA : 0.02%
Medium
RPMI 1640
Colon
Mouse
Mouse
Larmonier, C. , Shehab, K. , Laubitz, D. , Jamwal, D. , Ghishan, F. and Kiela, P.
Transcriptional Reprogramming and Resistance to Colonic Mucosal Injury in Poly(ADP-ribose) Polymerase 1 (PARP1)-deficient Mice. ,
J Biol Chem
 291 , 8918-30 , 2016
Cells
Lamina propria
Enzymes
Collagenase : 100 u/ml
Medium
RPMI 1640
Colon
Mouse
Mouse
Atarashi, K. , Nishimura, J. , Shima, T. , Umesaki, Y. , Yamamoto, M. , Onoue, M. , Yagita, H. , Ishii, N. , Evans, R. , Honda, K. and Takeda, K.
ATP Drives Lamina Propria T(H)17 Cell Differentiation. ,
Nature
 455 , 808 , 2008
Cells
Lamina propria
Enzymes
Medium
RPMI 1640
Colon
Mouse
Mouse
Weigmann Benno, Tubbe Ingrid, Seidel Daniel, Nicolaev Alex, Becker Christoph, Neurath Markus F.
Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue ,
Nat Protoc
 2 , 2307-11 , 2007
Cells
Lamina propria mononuclear cells
Enzymes
Medium
HBSS
Colon
Mouse
Mouse
Abe, K. , Nguyen, K. , Fine, S. , Mo, J. , Shen, C. , Shenouda, S. , Corr, M. , Jung, S. , Lee, J. , Eckmann, L. and Raz, E.
Conventional Dendritic Cells Regulate the Outcome of Colonic Inflammation Independently of T Cells. ,
Proc Natl Acad Sci U S A
 104 , 17022 , 2007
Cells
Dentritic
Enzymes
Collagenase : 300 u/ml
Medium
RPMI 1640
Colon
Mouse
Mouse, 6-8 week
Dalerba Piero, Dylla Scott J, Park In-Kyung, Liu Rui, Wang Xinhao, Cho Robert W, Hoey Timothy, Gurney Austin, Huang Emina H, Simeone Diane M, Shelton Andrew A, Parmiani Giorgio, Castelli Chiara, Clarke Michael F.
Phenotypic characterization of human colorectal cancer stem cells ,
Proc Natl Acad Sci U S A
 104 , 10158-63 , 2007
Cells
Cancer stem cell
Enzymes
Collagenase Type 3 : 200 u/ml
Deoxyribonuclease I : 100 u/ml
Medium
RPMI-1640
Colon
Mouse
Mouse, 6-8 week
Totsuka T, Kanai T, Uraushihara K, Iiyama R, Yamazaki M, Akiba H, Yagita H, Okumura K, Watanabe M.
Therapeutic effect of anti-OX40L and anti-TNF-alpha MAbs in a murine model of chronic colitis ,
Am J Physiol/Gastro
 284 , G595-603 , 2003
Cells
Lamina propria lymphocytes
Enzymes
Medium
HBSS
Colon
Mouse
Mouse, 6-8 week
Wirtz S. , Becker C. , Blumberg R. , Galle P. and Neurath M.
Treatment of T cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing IL-18 antisense mRNA ,
J Immunol
 168(1) , 411 , 2002
Cells
Lamina propria mononuclear cells
Enzymes
Medium
RPMI
Kidney
Canine
Dog, mongrel, 20 Kg
Yau, C. , Rao, L. and Silverman, M.
Sugar Uptake Into a Primary Culture of Dog Kidney Proximal Tubular Cells ,
Can J Physiol Pharmacol
 63 , 417 , 1985
Cells
Proximal tubular
Enzymes
Medium
(see reference)
Kidney
Mouse

Mouse

Li, Z. , Zimmerman, K. , Cherakara, S. , Chumley, P. , Collawn, J. , Wang, J. , Haycraft, C. , Song, C. , Chacana, T. , Andersen, R. , Croyle, M. , Aloria, E. , Hombal, R. , Thomas, I. , Chweih, H. , Simanyi, K. , George, J. , Parant, J. , Mrug, M. and Yoder, B.
A Kidney Resident Macrophage Subset is a Candidate Biomarker for Renal Cystic Disease in Preclinical Models. , Dis Model Mech Vol. 16 , 2023
Cells
Macrophages
Enzymes
Medium
RPMI 1640
Kidney
Mouse
Mouse
Yuan, Y. , Li, L. , Zhu, L. , Liu, F. , Tang, X. , Liao, G. , Liu, J. , Cheng, J. , Chen, Y. and Lu, Y.
Mesenchymal Stem Cells Elicit Macrophages into M2 Phenotype Via Improving Transcription Factor EB-Mediated Autophagy to Alleviate Diabetic Nephropathy. ,
Stem Cells
 38 , 639-652 , 2020
Cells
Mactrophages
Enzymes
Medium
DMEM
Kidney
Mouse
Mouse
Adam, M. , Potter, A. and Potter, S.
Psychrophilic Proteases Dramatically Reduce Single-Cell RNA-Seq Artifacts: A Molecular Atlas of Kidney Development. ,
Development
 144 , 3625-3632 , 2017
Cells
Kidney
Enzymes
Deoxyribonuclease I : 125 u/ml
Protease, Bacillus Licheniformis : 1%
Medium
DPBS
Kidney
Mouse
Mouse
Sedrakyan, S. , Da Sacco, S. , Milanesi, A. , Shiri, L. , Petrosyan, A. , Varimezova, R. , Warburton, D. , Lemley, K. , De Filippo, R. and Perin, L.
Injection of Amniotic Fluid Stem Cells Delays Progression of Renal Fibrosis. ,
J Am Soc Nephrol
 23 , 661-73 , 2012
Cells
Glomeruli
Enzymes
Medium
DMEM
Kidney
Mouse
Mouse, embryonic
Brown, A. , Blank, U. , Adams, D. , Karolak, M. , Fetting, J. , Hill, B. and Oxburgh, L.
Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney. ,
J Vis Exp
 50 , e2555 , 2011
Cells
Kidney
Enzymes
Medium
HBSS
Kidney
Mouse
Mouse, naive SJL (H-2S)
Haverty, T. , Kelly, C. , Hines, W. , Amenta, P. , Watanabe, M. , Harper, R. , Kefalides, N. and Neilson, E.
Characterization of a Renal Tubular Epithelial Cell Line Which Secretes the Autologous Target Antigen of Autoimmune Experimental Interstitial Nephritis ,
J Cell Biol
 107 , 1359 , 1988
Cells
Proximal tubular epithelial
Enzymes
Deoxyribonuclease I : 15 µg/ml
Medium
RPMI 1640
Kidney
Rabbit
Rabbit, New Zealand White, female
Schafer, J. , Watkins, M. , Li, L. , Herter, P. , Haxelmans, S. and Schlatter, E.
A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments ,
Am J Physiol
 273 , F650 , 1997
Cells
Nephron segments
Enzymes
Medium
Eagle's MEM
Kidney
Rabbit
Rabbit, New Zealand white, female, 2-3 Kg
Rodeheaver, D. , Aleo, M. and Schnellmann, R.
Differences in Enzymatic and Mechanical Isolated Rabbit Renal Proximal Tubules: Comparison in Long-Term Incubation ,
In Vitro Cell Dev Biol
 26 , 898 , 1990
Cells
Renal proximal tubules
Enzymes
Medium
Modified DME-F12
Kidney
Rat
Rat, SD, male
Schafer, J. , Watkins, M. , Li, L. , Herter, P. , Haxelmans, S. and Schlatter, E.
A Simplified Method for Isolation of Large Numbers of Defined Nephron Segments ,
Am J Physiol
 273 , F650 , 1997
Cells
Nephron segments
Enzymes
Medium
Eagle's MEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.