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Tissue References Search Tool

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Pancreas
Canine
Canine, adult mongrel, 15-25 kg
Noel, J. , Rabinovitch, A. , Olson, L. , Kyriakides, G. , Miller, J. and Mintz, D.
A Method for Large-Scale, High-Yield Isolation of Canine Pancreatic Islets of Langerhans ,
Metabolism
 31 (2) , 184 , 1982
Cells
Islets
Enzymes
Collagenase Type 4 : 600-1100 u/ml
Deoxyribonuclease I : 10 ug/ml
Medium
RPMI 1640
Pancreas
Human
Human
Brissova, M. , Haliyur, R. , Saunders, D. , Shrestha, S. , Dai, C. , Blodgett, D. , Bottino, R. , Campbell-Thompson, M. , Aramandla, R. , Poffenberger, G. , Lindner, J. , Pan, F. , von Herrath, M. , Greiner, D. and Shultz, L.
α Cell Function and Gene Expression Are Compromised in Type 1 Diabetes. ,
Cell Rep
 22 , 2667-2676 , 2018
Cells
Islets
Enzymes
Collagenase Type 1 : 1,600u/isolation
Neutral Protease : 200 u/isolation
Deoxyribonuclease I : 12,000 u/isolation
Medium
RPMI
Pancreas
Human
Human
Watt, P. , Mullen, Y. , Benhamou, P. , Hober, C. , Nomura, Y. , Watanabe, Y. , Passaro, E. , Zinner, M. and Brunicardi, F.
Simplified Semiautomated Method For Isolating Islets From the Human Pancreas ,
Transplant Proc
 26 (2) , 582 , 1994
Cells
Islets
Enzymes
Trypsin : Trypsin: 0.125-0.25%
Collagenase : 0.2%
Deoxyribonuclease I : 200 u/ml
Medium
Eurocollins solution
Tonsil
Human
Human, female, 25-45 year
Grammer Amrie C, Slota Rebecca, Fischer Randy, Gur Hanan, Girschick Hermann, Yarboro Cheryl, Illei Gabor G, Lipsky PeterE.
Abnormal germinal center reactions in systemic lupus erythematosus demonstrated by blockade of CD154-CD40 interactions ,
J Clin Invest
 112 , 1506-20 , 2003
Cells
Tonsillar mononuclear cells
Enzymes
Medium
RPMI
Tonsil
Human
Human
Grammer AC, McFarland RD, Heaney J, Darnell BF, Lipsky PE.
Expression, regulation, and function of B cell-expressed CD154 in germinal centers ,
J Immunol
 163 , 4150-9 , 1999
Cells
Tonsillar mononuclear cells
Enzymes
Medium
RPMI

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.