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Tissue References Search Tool

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Heart
Human
Human
Jensen, B. , Swigart, P. , Laden, M. , DeMarco, T. , Hoopes, C. and Simpson, P.
The Alpha-1D Is the Predominant Alpha-1-Adrenergic Receptor Subtype in Human Epicardial Coronary Arteries. ,
J Am Coll Cardiol
 54 , 1137 , 2009
Cells
Coronary artery smooth muscle
Enzymes
Elastase : 0.05%
Medium
DMEM
Heart
Human
Human
Mathew, S. , Tustison, K. , Sugatani, T. , Chaudhary, L. , Rifas, L. and Hruska, K.
The Mechanism of Phosphorus as a Cardiovascular Risk Factor in CKD. ,
J Am Soc Nephrol
 19 , 1092 , 2008
Cells
Smooth muscle aortic
Enzymes
Medium
DMEM
Heart
Mouse
Mouse, 2-6 month
Christel, C. , Cardona, N. , Mesirca, P. , Herrmann, S. , Hofmann, F. , Striessnig, J. , Ludwig, A. , Mangoni, M. and Lee, A.
Distinct Localization and Modulation of Cav1.2 and Cav1.3 L-type Ca2+ Channels in Mouse Sinoatrial Node. ,
J Physiol
 590 , 6327 , 2012
Cells
Sinoatrial node
Enzymes
Collagenase Type 2 : 229 u/ml
Elastase : 1.9 u/ml
Protease Type XIV : 0.9 u/ml
Medium
Tyrode's solution
Heart
Mouse
Mouse
Butcher, M. , Herre, M. , Ley, K. and Galkina, E.
Flow Cytometry Analysis of Immune Cells Within Murine Aortas. ,
J Vis Exp
 53 , e2828 , 2011
Cells
Aortic adventitial leukocytes
Enzymes
Collagenase Type 2 : 300 u/ml
Elastase : 5.6 u/ml
Medium
PBS
Heart
Mouse
Mouse
Verheye, S. , Martinet, W. , Kockx, M. , Knaapen, M. , Salu, K. , Timmermans, J. , Ellis, J. , Kilpatrick, D. and DeMayer, G.
Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy ,
J Am Coll Cardiol
 49 , 706 , 2007
Cells
Smooth muscle cells
Enzymes
Collagenase Type 2 : 300 u/ml
Elastase : 5 u/ml
Medium
F10 Ham
Heart
Mouse
Mouse
Fukumoto Yoshihiro, Deguchi Jun-o, Libby Peter, Rabkin-Aikawa Elena, Sakata Yasuhiko, Chin Michael T, Hill Christopher C, Lawler Patrick R, Varo Nerea, Schoen Frederick J, Krane Stephen M, Aikawa Masanori.
Genetically determined resistance to collagenase action augments interstitial collagen accumulation in atherosclerotic plaques ,
Circulation
 110 , 1953-9 , 2004
Cells
Smooth muscle cells
Enzymes
Elastase : 0.0125%
Medium
DMEM
Heart
Mouse
Mouse, female, 14-18 g
Zhang Sui, Wang Dachun, Estrov Zeev, Raj Sean, Willerson James T, Yeh Edward T H.
Both cell fusion and transdifferentiation account for the transformation of human peripheral blood CD34-positive cells into cardiomyocytes in vivo ,
Circulation
 110 , 3803-7 , 2004
Cells
Cardiomyocytes
Enzymes
Elastase : 0.03%
Pancreatin : 0.06%
Medium
see reference
Heart
Mouse
Mouse, 6-8 week
Qian, Q. , Hunter, L. , Li, M. , Marin-Padilla, M. , Prakash, Y. , Somlo, S. , Harris, P. , Torres, V. and Sieck, G.
Pkd2 Haploinsufficiency Alters Intracellular Calcium Regulation in Vascular Smooth Muscle Cells ,
Hum Mol Genet
 12(15) , 1875 , 2003
Cells
Vascular smooth muscle
Enzymes
Papain : 10 u/ml
Elastase : .005%
Collagenase : 0.05%
Deoxyribonuclease I : 1000 u/ml
PDS kit : with modifications
Medium
EBSS
Heart
Rabbit
Rabbit
Verheye, S. , Martinet, W. , Kockx, M. , Knaapen, M. , Salu, K. , Timmermans, J. , Ellis, J. , Kilpatrick, D. and DeMayer, G.
Selective Clearance of Macrophages in Atherosclerotic Plaques by Autophagy ,
J Am Coll Cardiol
 49 , 706 , 2007
Cells
Smooth muscle cells
Enzymes
Collagenase Type 2 : 300 u/ml
Elastase : 5 u/ml
Medium
F10 Ham
Heart
Rat
Rat, adult
Zhang, Y. , Wang, Y. , Yanni, J. , Qureshi, M. , Logantha, S. , Kassab, S. , Boyett, M. , Gardiner, N. , Sun, H. , Howarth, F. and Dobrzynski, H.
Electrical Conduction System Remodeling in Streptozotocin-Induced Diabetes Mellitus Rat Heart. ,
Front Physiol
 10 , 826 , 2019
Cells
Sinoatrial nodal
Enzymes
Collagenase Type 4 : 220 u/ml
Elastase : 1.4 u/ml
Protease : 0.45 u/ml
Medium
Tyrode solution
Heart
Rat
Rat, SD, adult female
Puri S, Bansal DD, Uskokovic MR. and MacGregor RR.
Induction of tissue plasminogen activator secretion from rat heart microvascular cells by fM 1,25(OH)(2)D(3) ,
Am J Physiol/Endo
 278(2) , E293 , 2000
Cells
Heart microvascular cells
Enzymes
CLSPA : 250u/ml
Papain : 5 u/ml
Elastase : 0.8 u/ml
Medium
L-15
Heart
Rat
Rat
Zilberman, A. , Dave, V. , Miano, J. , Olson, E. and Periasamy, M.
Evolutionary Conserved Promoter Region Containing CArg-Like Elements is Crucial for Smooth Muscle Myosin Heavy Chain Gene Expression ,
Circ Res
 82 , 566 , 1998
Cells
Thoracic aorta, smooth muscle
Enzymes
Elastase : 0.02%
Medium
(see reference)
Muscle
Canine
Dog
Subramanian, M. , Madden, J. and Harder, D.
A Method for the Isolation of Cells from Arteries of Various Sizes ,
J Tiss Cul Meth
 13 , 13 , 1991
Cells
Smooth muscle
Enzymes
Elastase : 50 u/ml
Medium
PSS
Muscle
Canine
Dog, beagle, adult
Wilde, D. and Lee, K.
Outward Potassium Currents in Freshly Isolated Smooth Muscle Cell of Dog Coronary Arteries ,
Circ Res
 65 , 1718 , 1989
Cells
Smooth muscle
Vascular
Enzymes
Elastase : 34 u/ml
Medium
Tyrode's solution w/ calcium
Muscle
Canine
Dog
Dobrin, P. and Canfield, T.
Elastase, Collagenase, and the Biaxial Elastic Properties of Dog Carotid Artery ,
Am J Physiol Heart Circ Physiol
 247 (16) , H124 , 1984
Cells
Artery
Carotid
Enzymes
Elastase : 80 u/ml
Medium
PSS
Muscle
Feline
Cat, adult mongrel, either sex, 2.5-4.0 kg
Madden, J. , Vadula, M. and Kurup, V.
Effects of Hypoxia and Other Vasoactive Agents on Pulmonary and Cerebral Artery Smooth Muscle Cells ,
Am J Physiol
 263 , L384 , 1992
Cells
Cerebral arteries
Enzymes
Elastase : 50 u/ml
Medium
Puck's solution
Muscle
Monkey
Rhesus monkey, 1 year
(also human, rabbits)
Chamley, J. , Campbell, G. , McConnell, J. and Groschel-Stewart, U.
Comparison of Vascular Smooth Muscle Cells from Adult Human, Monkey and Rabbit in Primary Culture and in Subculture ,
Cell Tissue Res
 177 , 503 , 1977
Cells
Smooth muscle, saphenous vein
Enzymes
Elastase : 0.05%
Medium
BSS
Muscle
Mouse
Mouse
Eckhardt, J. , Bachmann, C. , Sekulic-Jablanovic, M. , Enzmann, V. , Park, K. , Ma, J. , Takeshima, H. , Zorzato, F. and Treves, S.
Extraocular Muscle Function is Impaired in ryr3−/− Mice ,
J Gen Physiol
 151 , 929-943 , 2019
Cells
Extraocular muscles
Enzymes
Elastase : 0.0125-0.1%
Collagenase Type 2 : 0.03-0.2%
Medium
Ringer
Muscle
Mouse
Mouse, 6-8 week
Sachdeva, J. , Mahajan, A. , Cheng, J. , Baeten, J. , Lilly, B. , Kuivaniemi, H. and Hans, C.
Smooth Muscle Cell-Specific Notch1 Haploinsufficiency Restricts the Progression of Abdominal Aortic Aneurysm by Modulating CTGF Expression. ,
PLoS ONE
 12 , e0178538 , 2017
Cells
vascular smooth muscle
Enzymes
Elastase : 0.75 u/ml
Medium
DMEM/F12
Muscle
Porcine
Porcine, male, 25-40 kg
Korzick, D. , Laughlin, M. and Bowles, D.
Alterations in PKC signaling underlie enhanced myogenic tone in exercise-trained porcine coronary resistance arteries ,
J Appl Physiol
 96 , 1425-32 , 2004
Cells
Coronary myocytes
Enzymes
Medium
low calcium physiological saline
Muscle
Porcine
Porcine, adult, 35-45 kg
Wamhoff BR, Dixon JL, Sturek M.
Atorvastatin treatment prevents alterations in coronary smooth muscle nuclear Ca2+ signaling in diabetic dyslipidemia ,
J Vasc Res
 39 , 208 , 2002
Cells
Arterial smooth muscle
Enzymes
Collagenase Type 2 : 294 u/ml
Elastase : 6.5 u/ml
Deoxyribonuclease I : 0.4 mg/ml
Medium
MEM
Muscle
Porcine
Porcine, Yorkshire, 30kg
Sirous ZN, Fleming JB, Khalil RA.
Endothelin-1 enhances eicosanoids-induced coronary smooth muscle contraction by activating specific protein kinase C isoforms ,
Hypertension
 37 , 497-504 , 2001
Cells
Coronary smooth muscle
Enzymes
Collagenase Type 2 : 150 u/ml
Elastase : 0.05%
Medium
HBSS
Muscle
Porcine
Porcine, juvenile, 30kg
Huckle WR, Drag MD, Acker WR, Powers M, McFall RC, Holder DJ, Fujita T, Stabilito II, Kim D, Ondeyka DL, Mantlo NB, Chang RS, Reilly CF, Schwartz RS, Greenlee WJ, Johnson RG.
Effects of subtype-selective and balanced angiotensin II receptor antagonists in a porcine coronary artery model of vascular restenosis ,
Circulation
 93 , 1009-19 , 1996
Cells
Coronary smooth muscle cells
Enzymes
Collagenase : 0.3%
Elastase : 0.05%
Medium
HBSS
Muscle
Rabbit
Rabbit, New Zealand White
Croons Valerie, Martinet Wim, Herman Arnold G, Timmermans Jean-Pierre, De Meyer Guido R Y.
Selective clearance of macrophages in atherosclerotic plaques by the protein synthesis inhibitor cycloheximide ,
J Pharmacol Exp Ther
 320 , 986-93 , 2007
Cells
Aortic smooth muscle
Enzymes
Collagenase Type 2 : 300 u/ml
Elastase : 5 u/ml
Medium
F10 Ham's
Muscle
Rabbit
Rabbit, adult, 1-2 kg
Benham, C. , Bolton, T. , Byrne, N. and Large, W.
Action of Extremely Applied Adenosine Triphosphate O Single Smooth Muscle Cells Dispersed From Rabbit Ear Artery ,
J Physiol
 387 , 473 , 1987
Cells
Smooth muscle
Enzymes
Elastase : 0.17 - 0.25%
Medium
Saline
Muscle
Rabbit
Rabbits, New Zealand white albino, 5-6 months
(also human, Rhesus monkey, rabbit)
Chamley, J. , Campbell, G. , McConnell, J. and Groschel-Stewart, U.
Comparison of Vascular Smooth Muscle Cells from Adult Human, Monkey and Rabbit in Primary Culture and in Subculture ,
Cell Tissue Res
 177 , 503 , 1977
Cells
Smooth muscle, saphenous vein
Enzymes
Elastase : 0.05%
Medium
BSS
Muscle
Rabbit
Rabbit, New England, albino
Day, A. , Phil, D. and Newman, H.
Synthesis of Phospholipid by Foam Cells Isolated from Rabbit Atherosclerotic Lesions ,
Circ Res
 XIX , 122 , 1966
Cells
Thoracic aorta
Enzymes
Elastase : 0.008%
Medium
Kreb's Ringer
Muscle
Rat
Rat, SD
Zeng, Y. , Liu, H. , Kang, K. , Wang, Z. , Hui, G. , Zhang, X. , Zhong, J. , Peng, W. , Ramchandran, R. , Raj, J. and Gou, D.
Hypoxia Inducible Factor-1 Mediates Expression of miR-322: Potential Role in Proliferation and Migration of Pulmonary Arterial Smooth Muscle Cells. ,
Sci Rep
 5 , 12098 , 2015
Cells
Pulmonary arterial smooth muscle
Enzymes
Elastase : 0.05%
Medium
Buffer
Muscle
Rat
Rat
Weber, S. , Gratopp, A. , Akanbi, S. , Rheinlaender, C. , Sallmon, H. , Barikbin, P. and Koehne, P.
Isolation and Culture of Fibroblasts, Vascular Smooth Muscle, and Endothelial Cells From the Fetal Rat Ductus Arteriosus ,
Pediatr Res
 70 , 236 , 2011
Cells
Vascular smooth muscle
Enzymes
Medium
DMEM
Muscle
Rat
Rat, SD, 3 month
Stevenson S. , Rollence M. , Marshall-Neff J. and Liau G.
A Genetically Modified Adenoviral Vector Exhibits Enhanced Gene Transfer of Human Smooth Muscle Cells ,
J Vasc Res
 38(5) , 471 , 2001
Cells
Smooth muscle cells
Enzymes
Medium
M-199
Muscle
Rat
Rat, SD, male, 250-350 g
Hrometz, S. , Edelmann, S. , McCune, D. , Olges, J. , Hadley, R. , Perez ,D. and Piascik, M.
Expression of Multiple Alpha1-Adrenoceptors on Vascular Smooth Muscle: Correlation with the Regulation of Contraction ,
J Pharmacol Exp Ther
 290(1) , 452 , 1999
Cells
Vascular smooth muscle
Enzymes
Elastase : 0.0125%
Medium
DMEM
Muscle
Rat
Rat, 1-3 day
Barone, L. , Wolfe, L. , Faris, B. and Franzblau, C.
Elastin mRNA Levels and Insoluble Elastin Accumulation in Neonatal Rat ,
Biochemistry
 27 , 3175 , 1988
Cells
Smooth muscle, aortic
Enzymes
Elastase : 0.0125%
Medium
DMEM
Muscle
Rat
Rat, SHR, WKY. either sex, 12-19 day, 3 month, and retired breeders
Gordon, D. , Mohai, L. and Schwartz, S.
Induction of Polyploidy in Cultures of Neonatal Rat Aortic Smooth Muscle Cells ,
Circ Res
 59 , 633 , 1986
Cells
Endothelial, aortic
Enzymes
Elastase : 0.05%
Medium
Waymouth's culture medium
Muscle
Rat
Rat, SD, 225-250g
Gunther S, Alexander RW, Atkinson WJ. and Gimbrone MA Jr.
Functional angiotensin II receptors in cultured vascular smooth muscle cells ,
J Cell Biol
 92 , 289 , 1982
Cells
Mesenteric artery smooth muscle cells
Enzymes
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.