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Tissue References Search Tool

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Mammary
Mouse
Mouse (BALB/cCrgl), mature
Bandyopadhyay, G. , Imagawa, W. , Wallace, D. and Nandi, S.
Proliferative effects of insulin and epidermal growth factor on mouse mammary epithelial cells in primary culture ,
J Biol Chem
 263 , 7567 , 1988
Cells
Epithelial
Enzymes
Pronase : 0.01%
Medium
Hepes buffered medium 199
Mammary
Mouse
Mouse (BALB/c), pregnant, 60-80 days
Ceriani, R. , Peterson, J. and Abraham, S.
Removal of Cell Surface Material by Enzymes Used to Dissociate Mammary Gland Cells ,
In Vitro
 14 , 887 , 1978
Cells
Mammary
Enzymes
Hyaluronidase : 0.1%
Medium
CMF medium
Mammary
Mouse
Mouse, BALB/cfC3H/Crgl, virgin, female
DeOme, K. , Miyamoto, M. , Osborn, R. , Guzman, R. and Lum, K.
Detection of Inaparent Nodule-transformed Cells in the Mammary Gland Tissues of Virgn Female BALB/cfC3H Mice ,
Cancer Res
 38 , 2103 , 1978
Cells
Nodule-transformed
Enzymes
Hyaluronidase : 0.1%
Medium
Medium 199
Mammary
Mouse
Mouse, CBA, virgin, 9/10 wk
Prop, F. and Wiepjes, G.
Improved method for preparation of single-cell suspensions from mammary glands of adult virgin mouse ,
Exp Cell Res
 61 , 451 , 1970
Cells
Mammary
Enzymes
Hyaluronidase : 0.1%
Medium
BSS, CMF
Mammary
Rat
Rat, Fisher 344, virgin, 80-100 day
McGrath, M. , Palmer, S. and Nandi, S.
Differential Response of Normal Rat Mammary Epithelial Cells to Mammogenic Hormones and EGF ,
J Cell Physiol
 125 , 182 , 1985
Cells
Epithelial
Enzymes
Hyaluronidase : 0.1%
Medium
Medium 199
Miscellaneous
Canine

Canine, Rat, Rabbit, Ovine, Bovine, Human

Basatvat, S. , Bach, F. , Barcellona, M. , Binch, A. , Buckley, C. , Bueno, B. , Chahine, N. , Chee, A. and Maitre, C.
Harmonization and Standardization of Nucleus Pulposus Cell Extraction and Culture Methods. ,
Spine
 6 , e1238 , 2023
Cells
nucleus pulposus
Enzymes
Pronase : 0.075%
Medium
DMEM
Miscellaneous
Human
Human
Sun, Y. , Lv, M. , Zhou, L. , Tam, V. , Lv, F. , Chan, D. , Wang, H. , Zheng, Z. , Cheung, K. and Leung, V.
Enrichment of Committed Human Nucleus Pulposus Cells Expressing Chondroitin Sulfate Proteoglycans under Alginate Encapsulation. ,
Osteoarthritis Cartilage
 23 , 1194-203 , 2015
Cells
Nucleus pulposus
Enzymes
Pronase : 0.25%
Collagenase Type 2 : 600 u/ml
Medium
DMEM
Miscellaneous
Insect
Insect, Lepidoptera
Goodwin, R. and McCawley, P
Initiating Attached Cell Lines From the Lepidoptera (Insecta) ,
Meth Cell Sci
 3 , 567 , 1977
Cells
Lepitopteran
Enzymes
Medium
Dulbecco PBS
Miscellaneous
Porcine
Porcine, 6 month
Kean, T. and Dennis, J.
Synoviocyte Derived-Extracellular Matrix Enhances Human Articular Chondrocyte Proliferation and Maintains Re-Differentiation Capacity at Both Low and Atmospheric Oxygen Tensions. ,
PLoS ONE
 10 , e0129961 , 2015
Cells
Synoviocytes
Enzymes
Hyaluronidase : 660 u/ml
Trypsin : 0.25%
Collagenase Type 2 : 583 u/ml
Medium
DMEM
Miscellaneous
Porcine
Porcine, <10 week
Potier, E. and Ito. K.
Can Notochordal Cells Promote Bone Marrow Stromal Cell Potential for Nucleus Pulposus Enrichment? A Simplified in Vitro System. ,
Tissue Eng Part A
 20 , 3241-51 , 2014
Cells
Nucleus pulposus
Enzymes
Collagenase : 0.05%
Pronase : 0.3%
Medium
DMEM
Miscellaneous
Porcine
Porcine, various ages
Cho, H. , Park, S. , Lee, S. , Kang, M. , Hasty, K. and Kim, S.
Snapshot of Degenerative Aging of Porcine Intervertebral Disc: A Model to Unravel the Molecular Mechanisms. ,
Exp Mol Med
 43 , 334-40 , 2011
Cells
Nucleous pulposus and AF
Enzymes
Pronase : 0.05%
Collagenase : 0.2%
Medium
Modified F-12K
Muscle
Mouse
Mouse, 10 week
Rezk, B. , Yoshida, T. , Semprun-Prieto, L. , Higashi, Y. , Sukhanov, S. and Delafontaine, P.
Angiotensin II Infusion Induces Marked Diaphragmatic Skeletal Muscle Atrophy. ,
PLoS ONE
 7 , e30276 , 2012
Cells
Diaphragm cells
Enzymes
Collagenase Type 2 : 100 u/ml
Pronase : 0.125%
Medium
PBS
Muscle
Rabbit
Rabbit, chinchilla, 5-8 month, virgin, female, 2-3 kg
Peters, T. , Muller, M. and deDuve, C.
Lysosomes of the Arterial Wall. I. Isolation and Subcellular Fractionation of Cells from Normal Rabbit Aorta ,
J Exp Med
 136 , 1117 , 1972
Cells
Smooth muscle, aorta
Enzymes
Hyaluronidase : 800 u/ml
Medium
HBSS
Neural
Canine
Canine
Tongtako, W. , Lehmbecker, A. , Wang, Y. , Hahn, K. , Baumgartner, W. and Gerhauser, I.
Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties. ,
Sci Rep
 7 , 13915 , 2017
Cells
Satellite glial
Enzymes
Medium
HBSS
Neural
Chicken
Chick, White Leghorn, embryos, 7 day
Wiseman, L. and Hammond, W.
The Reacquisition of Cell Adhesiveness Following Tissue Disaggregation by Eleven Different Agents ,
J Exp Zool
 197 , 429 , 1976
Cells
Neural retina
Enzymes
Collagenase : 0.25%
Elastase : 0.2%
Hyaluronidase : 1.0%
Papain : 1.0%
Protease : 0.1%
Trypsin : 0.05%
Medium
HBSS
Neural
Mouse
Mouse, embryonic 12.5
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Neural
Mouse
Mouse
Xu, W. , Sachewsky, N. , Azimi, A. , Hung, M. , Gappasov, A. and Morshead, C.
Myelin Basic Protein Regulates Primitive and Definitive Neural Stem Cell Proliferation from the Adult Spinal Cord. ,
Stem Cells
 35 , 485-496 , 2017
Cells
Neurospheres
Enzymes
Trypsin : 0.13%
Hyaluronidase : 0.08%
Medium
Neurobasal A
Neural
Mouse
Mouse, 4 month
Tongtako, W. , Lehmbecker, A. , Wang, Y. , Hahn, K. , Baumgartner, W. and Gerhauser, I.
Canine Dorsal Root Ganglia Satellite Glial Cells Represent an Exceptional Cell Population with Astrocytic and Oligodendrocytic Properties. ,
Sci Rep
 7 , 13915 , 2017
Cells
Satellite glial
Enzymes
Medium
HBSS
Neural
Mouse
Mouse, 6-8 week
Babona-Pilipos, R. , Popovic, M. and Morshead, C.
A Galvanotaxis Assay for Analysis of Neural Precursor Cell Migration Kinetics in an Externally Applied Direct Current Electric Field. ,
J Vis Exp
 68 , e4193 , 2012
Cells
Neural precursors
Enzymes
Trypsin : 0.13%
Hyaluronidase : 0.08%
Medium
see reference
Neural
Rat
Rat, embryonic 14.5 day
Mirsky, R. and Jessen, K.
Isolation of Schwann Cell Precursors from Rodents. ,
Methods Mol Biol
 1739 , 3-15 , 2018
Cells
Schwann cell precursors
Enzymes
Medium
DMEM/ Ham's F-12
Organoids
Human

Human

Vaart, J. , Böttinger, L. , Geurts, M. , Wetering, W. , Knoops, K. , Sachs, N. , Begthel, H. , Korving, J. , Lopez-Iglesias, C. , Peters, P. , Eitan, K. , Gileles-Hillel, A. and Clevers, H.
Modelling of Primary Ciliary Dyskinesia using Patient-derived Airway Organoids. , EMBO Rep Vol. 22 , e52058 , 2021
Cells
Airway organoids
Enzymes
Pronase : 0.15%
Collagenase : 0.05%
Medium
Airway medium
Organoids
Human

Human

Marinucci, M. , Ercan, C. , Taha-Mehlitz, S. , Fourie, L. , Panebianco, F. , Bianco, G. , Gallon, J. , Staubli, S. , Soysal, S. , Zettl, A. , Rauthe, S. , Vosbeck, J. , Droeser, R. , Bolli, M. , Peterli, R. , Flüe, M. , Ng, C. , Kollmar, O. , Coto-Llerena, M. and Piscuoglio, S.
Standardizing Patient-Derived Organoid Generation Workflow to Avoid Microbial Contamination From Colorectal Cancer Tissues. , Front Oncol Vol. 11 , 781833 , 2021
Cells
Colorectal cancer organoids
Enzymes
Medium
DMEM/F-12
Pancreas
Guinea-Pig
Guinea-pig
Amsterdam, J. and Jamieson, J.
Structural and Functional Characterization of Isolated Pancreatic Exocrine Cells ,
Proc Natl Acad Sci U S A
 69 (10) , 3028 , 1972
Cells
Exocrine
Enzymes
Hyaluronidase : 0.15% - 0.2%
Medium
Kreb's Ringer
Pancreas
Monkey
Monkey, 3-5 Kg
Scharp, D. , Murphy, J. , Newton, W. , Ballinger, W. and Lacy, P.
Application of an Improved Isolation Technique for Islet Transplantation in Primates and Rats ,
Transplant Proc
 7 , 739 , 1975
Cells
Islets
Enzymes
Hyaluronidase : 0.05%
Medium
HBSS
Pancreas
Mouse
Mouse, 6-10 month
Wang, Y. , Soyombo, A. , Shcheynikov, N. , Zeng, W. , Dorwart, M. , Marino, C. , Thomas, P. and Muallem, S.
Slc26a6 Regulates CFTR Activity In Vivo to Determine Pancreatic Duct HCO3- Secretion: Relevance to Cystic Fibrosis. ,
EMBO J
 25 , 5049 , 2006
Cells
Pancreatic ductal
Enzymes
CLSPA : 50 u/ml
Hyaluronidase : 400 u/ml
Medium
DMEM
Pancreas
Mouse
Mouse (C57BL/6J-ob/ob), 9-12 wks, male
Dalpe-Scott, M. , Heick, H. and Begin-Heick, N.
Secretion in the Obese (ob/ob) Mouse. The Effect of Oxytetracycline on Insulin Release ,
Diabetes
 32 , 932 , 1983
Cells
Islets
Enzymes
Hyaluronidase : 0.5%
Medium
Kreb's Ringer bicarbonate buffer
Pancreas
Rabbit
Rabbit, New Zealand white, Male/Female, 2-3 Kg
Renckens, B. , Schrijen, J. , Swarts, H. , DePont, J. and Bonting, S.
Role of Calcium in Exocrine Pancreatic Secretion. IV. Calcium Movements in Isolated Acinar Cells of Rabbit Pancreas ,
Biochim Biophys Acta
 544 , 338 , 1978
Cells
Acinar
Enzymes
Hyaluronidase : 0.2%
Medium
Kreb's Ringer bicarbonate buffer
Pancreas
Rat
Rat, SD, male, 40 - 100 g
Hirschi, K. , Kenny, S. , Justice, J. and Brannon, P.
Effects of Secretin And Caerulein On Enzymes Of Cultured Pancreatic Acinar Cells ,
In Vitro Cell Dev Biol
 27 , 660 , 1991
Cells
Acinar
Enzymes
Hyaluronidase : 462 u/ml
Medium
Ham's F12
Pancreas
Rat
Rat, Wistar, male, 250 - 350 g
Melvin, J. , Kawaguchi, M. , Baum, B. and Turner, R.
A Muscarinic Agonist-Stimulated Chloride Efflux Pathway is Associated With Fluid Secretion in Rat Parotid Acinar Cells ,
Biochem Biophys Res Commun
 145 , 754 , 1987
Cells
Acinar, parotid
Enzymes
Hyaluronidase : 0.015%
Medium
Earle's MEM
Pancreas
Rat
Rat, SD, male, 42 - 48 day, 175 - 200 g
Quissell, D. , Redman, R. and Mark, M.
Short-Term Primary Culture of Acinar-Intercalated Duct Complexes From Rat Submandibular Glands ,
In Vitro Cell Dev Biol
 22 , 469 , 1986
Cells
Acinar, submandibular gland
Enzymes
Hyaluronidase : 0.1 %
Medium
HBSS, CF
Pancreas
Rat
Rat, SD, male, 50-75 g
Brannon, P. , Orrison, B. and Kretchmer, N.
Primary Cultures of Rat Pancreatic Acinar Cells in Serum-Free Medium ,
In Vitro Cell Dev Biol
 21 (1) , 6 , 1985
Cells
Acinar
Enzymes
Hyaluronidase : 0.1%
Medium
HBSS
(see reference)
Pancreas
Rat
Rat
Schulz, I. , Heil, K. , Kribben, A. , Sachs, G. and Haase, W.
Isolation and Functional Characterization of Cells From the Exocrine Pancreas , Biology of Normal and Cancerous Exocrine Pancreatic Cells , Ribet, A., Pradayrol, L., and Susini, C. , Elsevier , 1980
Cells
Exocrine
Enzymes
Hyaluronidase : 0.9%
Medium
Kreb's Ringer
Pancreas
Rat
Rat, SD, male, 250 - 350 g
(also mouse, white, Swiss, male, 20 - 24 g)
Williams, J. , Korc, M. and Dormer, R.
Action of Secretagogues on a New Preparation of Functionally Intact, Isolated Pancreatic Acini ,
Am J Physiol
 235 , 517 , 1978
Cells
Acinar
Enzymes
Hyaluronidase : 0.18%
Medium
Kreb's Henseleit bicarbonate buffer
Pancreas
Rat
Rat
Kondo, S. and Schulz, I.
Calcium Ion Uptake in Isolated Pancreas Cells Induced by Secretagogues ,
Biochim Biophys Acta
 419 , 76 , 1976
Cells
Exocrine
Enzymes
Hyaluronidase : 0.15%
Medium
Krebs
Pancreas
Rat
Rat, SD, albino, male, 150-200 g
Mangos, J. , McSherry, N. , Butcher, F. , Irwin, K. and Barber, T.
Dispersed Rat Parotid Acinar Cells (Morphological, Functional Character) ,
Am J Physiol
 229 (3) , 560 , 1975
Cells
Acinar, parotid
Enzymes
Trypsin : 0.01%
Collagenase : 40-50 u/ml
Hyaluronidase : 0.10%
Medium
HBSS CMF
Parotid
Rat
Rat, Wistar, male, 130 g
Looms, D. , Dissing, S. , Tritsaris, K. , Pedersen, A. and Nauntofte, B.
Adrenoceptor-Activated Nitric Oxide Synthesis in Salivary Acinar Cells. ,
Adv Dent Res
 14 , 62 , 2000
Cells
Acinar
Enzymes
Collagenase : 75 u/ml
Hyaluronidase : 153 u/ml
Medium
RPMI 1640

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.