For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Tumor
Hamster
Hamster, 90-100 g
Gonzalez, A. , Oberley, T. , Schultz, J. , Ostrom, J. and Li, J.
In Vitro Charactrization of Estrogen Induced Syrian Hamster Renal Tumors: Comparison with an Immortalized Cell Line Derived from Diethylstilbestrol-Treated Adult Hamster Kidney ,
In Vitro Cell Dev Biol
 29A , 562 , 1993
Cells
Tumor
Enzymes
Hyaluronidase : 0.1%
Medium
Waymouth's MB
Tumor
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Tumor
Human
Human
Baldan, V. , Griffiths, R. , Hawkins, R. and Gilham, D
Efficient and Reproducible Generation of Tumour-Infiltrating Lymphocytes for Renal Cell Carcinoma ,
Br J Cancer
 112 , 1510 , 2015
Cells
Tumour infiltrating lymphocytes
Enzymes
Medium
RPMI 1640
Tumor
Human
Human
Ali, M. , Anand, S. , Tangella, K. , Ramkumar, D. and Saif, T.
Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry. ,
J Vis Exp
 , e52532 , 2015
Cells
Colon
Enzymes
Trypsin : 0.1%
Collagenase : 0.1%
Medium
HBSS
Tumor
Human
Human
Hasselbach, L. , Irtenkauf, S. , Lemke, N. , Nelson, K. , Berezovsky, A. , Carlton, E. , Transou, A. , Mikkelsen, T. and deCarvalho, A.
Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry. ,
J Vis Exp
 , e51088 , 2014
Cells
Glioblastoma tumor
Enzymes
Trypsin : 0.025%
Collagenase Type 4 : 500 u/ml
Medium
HBSS
Tumor
Human
Human
Dobbin, Z. and Landen, C.
Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications. ,
Curr Protoc Pharmacol
 63 , Unit 14.28. , 2013
Cells
Cancer stem
Enzymes
Trypsin : 0.25%
Collagenase : 0.05%
Hyaluronidase : 0.005%
Medium
RPMI
Tumor
Human
Human
Chou, J. , Fitzgibbon, M. , Mortales, C. , Towlerton, A. , Upton, M. , Yeung, R. , McIntosh, M. and Warren, E.
Phenotypic and Transcriptional Fidelity of Patient-Derived Colon Cancer Xenografts in Immune-Deficient Mice. ,
PLoS ONE
 8 , e79874 , 2013
Cells
Tumor
Enzymes
Medium
DMEM/F12
Tumor
Human
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Tumor
Human
Human
Quintana, E. , Shackleton, M. , Foster, H. , Fullen, D. , Sabel, M. , Johnson, T. and Morrison, S.
Phenotypic Heterogeneity Among Tumorigenic Melanoma Cells from Patients that is Reversible and Not Hierarchically Organized. ,
Cancer Cell
 18 , 510 , 2010
Cells
Tumorigenic melanoma
Enzymes
Collagenase Type 4 : 200 u/ml
Trypsin : 0.05%
Deoxyribonuclease I : 50-100 u/ml
Medium
PBS
Tumor
Human
Human
Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P.
Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. , Neuro Oncol Vol. 11 , 109 , 2009
Cells
Tumor
Enzymes
Medium
see reference
Tumor
Human
Human
Varnat, F. , Duquet, A. , Malerba, M. , Zbinden, M. , Mas, C. , Gervaz, P. and Ruiz i Altaba, A.
Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion. ,
EMBO Mol Med
 1 , 338-51 , 2009
Cells
Colon cancer
Enzymes
Collagenase Type 1 : 300 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM/F12
Tumor
Human
Human
MacLeod, R.
Rapid Monolayer Primary Cell Culture from Tissue Biopsy , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons Ltd , 3E:2.1 , 1995
Cells
Epithelial, fibroblasts
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Tumor
Human
Human
Hague, A. and Paraskeva, C
Colon Adenocarcinoma Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12C:1.1 , 1995
Cells
Colon adenocarcinoma
Enzymes
Hyaluronidase : 100 u/ml
Medium
DMEM
Tumor
Human
Human
Beaupain, R. , Mainquene, C. , Brouty-Boye, D. , Planchon, P. and Magniew, V.
"Normal" Breast Cells Adjacent to a Tumor Grown in Long-term Three Dimensional Culture ,
In Vitro Cell Dev Biol
 29 , 100 , 1993
Cells
Tumor, breast
Enzymes
Hyaluronidase : 100 u/ml
Medium
DMEM
Tumor
Human
Human
Kruse, C. , Mitchell, D. , Kleinschmidt-DeMasteis, B. , Franklin, W. , Morse, H. , Spector, E. and Lillehei, K.
Characterization of a Continuous Human Glioma Cell Line DBTRG-OSMG: Growth Kinetics, Karyotype, Receptor Expression and Tumor Suppressor Gene Analyses ,
In Vitro Cell Dev Biol
 28 , 609 , 1992
Cells
Glioma
Enzymes
Hyaluronidase : 0.01%
Medium
HBSS
Tumor
Human
Human, 9-74 year
Sheela S, Riccardi VM, Ratner N.
Angiogenic and invasive properties of neurofibroma Schwann cells ,
J Cell Biol
 111 , 645-53 , 1990
Cells
Neurofibroma
Enzymes
Medium
L-15
Tumor
Human
Human
Sacks, P. , Parnes, S. , Gallick, G. , Mansouri, Z. , Lichtner, R. , Satya-Prakash, K. , Pathak, S. and Parsons, D.
Establishment and Characterization of Two New Squamous Cell Carcinoma Cell Lines Derived from Tumors of the Head and Neck ,
Cancer Res
 48 , 2858 , 1988
Cells
Tumor
Enzymes
Trypsin : 0.05%
Medium
DMEM
Tumor
Human
Human
Brattain, M. , Marks, M. , McCombs, J. , Finely, W. and Brattain, D.
Characterization of Human Colon Carcinoma Cell Lines Isolated From a Single Primary Tumour ,
Br J Cancer
 47 , 373 , 1983
Cells
Tumor, colon
Enzymes
Trypsin : 0.25%
Medium
McCoy's
Tumor
Mouse
Mouse
Hosseini, H. , Obradovic, M. , Hoffmann, M. , Harper, K. , Sosa, M. , Werner-Klein, M. , Nanduri, L. , Werno, C. , Ehrl, C. , Maneck, M. , Patwary, N. , Haunschild, G. , Guzvic, M. and Reimelt, C. et al.
Early Dissemination Seeds Metastasis in Breast Cancer. ,
Nature
 , 2016
Cells
Mammary tumor
Enzymes
Collagenase Type 1 : 200 u/ml
Hyaluronidase : 0.001%
Medium
DMEM/F12
Tumor
Mouse
Mouse
Blanco, S. , Bandiera, R. , Popis, M. , Hussain, S. , Lombard, P. , Aleksic, J. , Sajini, A. , Tanna, H. , Cortes-Garrido, R. , Gkatza, N. , Dietmann, S. and Frye, M.
Stem Cell Function and Stress Response are Controlled by Protein Synthesis. ,
Nature
 534 , 335-40 , 2016
Cells
Keratinocytes
Enzymes
Medium
Low calcium containing media
Tumor
Mouse
Mouse
Kitajima, S. , Kohno, S. , Kondoh, A. , Sasaki, N. , Nishimoto, Y. , Li, F. , Mohammed, M. , Muranaka, H. , Nagatani, N. , Suzuki, M. , Kido, Y. and Takahashi, C.
Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts. ,
Stem Cells
 33 , 1657-69 , 2015
Cells
Thyroid tumor
Enzymes
Collagenase Type 1 : 300 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM
Tumor
Mouse
Mouse
Devaud, C. , Westwood, J. , John, L. , Flynn, J. , Paquet-Fifield, S. , Duong, C. , Yong, C. , Pegram, H. , Stacker, S. , Achen, M. Stewart, T. , Snyder, L. , Teng, M. , Smyth, M. , Darcy, P. and Kershaw, M
Tissues in Different Anatomical Sites can Sculpt and Vary the Tumor Microenvironment to Affect Responses to Therapy. ,
Mol Ther
 22 , 18-27 , 2014
Cells
Tumor
Enzymes
Medium
PBS
Tumor
Mouse
Mouse
Liu, X. , Johnson, S. , Liu, S. , Kanojia, D. , Yue, W. , Singh, U. , Wang, Q, Wang Qi, Nie, Q. and Chen H.
Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy. ,
Sci Rep
 3 , 2473 , 2013
Cells
Mammary tumor
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.020%
Medium
DMEM/F12
Tumor
Mouse
Mouse
Chen, X. , Stewart, E. , Shelat, A. , Qu, C. , Bahrami, A. , Hatley, M. , Wu, G. , Bradley, C. , McEvoy, J. , Pappo, A. , Spunt, S. , Valentine, M. , Valentine, V. , Krafcik, F. , Lang, W. , Wierdl, M. and Tsurkan, L.
Targeting Oxidative Stress in Embryonal Rhabdomyosarcoma. ,
Cancer Cell
 24 , 710-24 , 2013
Cells
Rhabdomycosarcoma tumor
Enzymes
Trypsin : 0.012%
Medium
DMEM
Tumor
Mouse
Mouse
Kwong, B. , Roberts, S. , Silberzahn, T. , Filler, R. , Neustadter, J. , Galan, A. , Reddy, S. , Lin, W. , Ellis, P. , Langford, C. , Hayday, A. and Girardi, M.
Molecular Analysis of Tumor-Promoting CD8+ T cells in Two-Stage Cutaneous Chemical Carcinogenesis. ,
J Invest Dermatol
 130 , 1726 , 2010
Cells
Tumor infiltration lymphocytes
Enzymes
Medium
RPMI 1640
Tumor
Mouse
Mouse, BALB/cfC3H/Crgl
Hosick, H.
A Note on Growth Patterns of Epithelial Tumor Cells in Primary Culture ,
Cancer Res
 34 , 259 , 1974
Cells
Neoplastic
Epithelial tumor
Medium
DMEM
Tumor
Rat
Rat, adult
Duarte, S. , Momier, D. , Baque, P. , Casanova, V. , Loubat, A. , Samson, M. , Guigonis, J. , Staccini, P. , Saint-Paul, M. , De Lima, M. , Carle, G. and Pierrefite-Carle, V.
Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model. ,
Stem Cells
 31 , 423-32 , 2013
Cells
Tumor
Enzymes
Medium
DMEM/F12
Tumor
Rat
Rat
Brennan, M. , Oldberg, A. , Hayman, E. and Ruoslahti, E.
Effect of a Proteoglycan Produced by Rat Tumor Cells on Their Adhesion to Fibronectin-Collagen Substrata ,
Cancer Res
 43 , 4302 , 1983
Cells
Yolk sac tumor
Enzymes
Trypsin : 0.01%
Medium
DMEM
Tumor
Rat
Rat
Gazdar, A. , Chick, W. , Oie, H. , Sims, H. , King, D. , Weir, G. and Lauris, V.
Continuous, Clonal, Insulin-and Somatostatin-Secreting Cell Lines Established from a Transplantable Rat Islet Cell Tumor ,
Proc Natl Acad Sci U S A
 77 (6) , 3519 , 1980
Cells
Tumor, islet
Enzymes
Trypsin : 0.05%
Medium
Medium 199
Tumor
Rat
Rat
Essner, E.
Experiments on an Ascites Hepatoma. I. Enzymatic Digestion and Alkaline Degradation of the Cementing Substance and Separation of Cells, in Tumor Islands ,
Exp Cell Res
 7 , 430 , 1954
Cells
Ascites hepatoma
Enzymes
Trypsin : 0.1%
Medium
Phosphate buffer (see reference)

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.