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Tissue References Search Tool

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Stem
Mouse
Mouse, postnatal
Sher, F. , Rossler, R. , Brouwer, N. , Balasubramaniyan, V. , Boddeke, E. and Copray, S.
Differentiation of Neural Stem Cells Into Oligodendrocytes: Involvement of the Polycomb Group Protein Ezh2. ,
Stem Cells
 26 , 2875 , 2008
Cells
Astrocytes
Enzymes
Trypsin : 0.25%
Deoxyribonuclease I : 1,000 u/ml
Medium
HBSS
Stem
Mouse
Mouse, 12 week
Taddei, I. , Deugnier, M. , Faraldo, M. , Petit, V. , Bouvard, D. , Medina, D. , Fassler, R. , Thiery, J. and Glukhova, M.
Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells ,
Nat Cell Biol
 10 , 716-22 , 2008
Cells
Mammary epithelial
Enzymes
Collagenase : 0.3%
Hyaluronidase : 100 u/ml
Trypsin : 0.25%
Medium
see reference
Stem
Mouse
Mouse, embryonic
Lu Shi-Jiang, Feng Qiang, Caballero Sergio, Chen Yu, Moore Malcolm A, Grant Maria B, Lanza Robert.
Generation of functional hemangioblasts from human embryonic stem cells ,
Nat Methods
 4 , 501-9 , 2007
Cells
HES-BC cells
Enzymes
Trypsin : 0.05%
Medium
DMEM
Stem
Mouse
Mouse, 6-8 week
McKinney-Freeman SL, Jackson KA, Camargo FD, Ferrari G, Mavilio F, Goodell MA.
Muscle-derived hematopoietic stem cells are hematopoietic in origin ,
Proc Natl Acad Sci U S A
 99 , 1341 , 2002
Cells
Myocytes
Enzymes
Trypsin : 0.25%
Medium
HBSS
Stem
Mouse
Mouse
Boulanger CA, Smith GH.
Reducing mammary cancer risk through premature stem cell senescence ,
Oncogene
 20 , 2264-72 , 2001
Cells
Mammary epithelial stem
Enzymes
Hyaluronidase : 0.1%
Pronase : 1.25%
Medium
DMEM
Stem
Mouse
Mouse, 6 week
Jackson, K. and Goodell, M.
Hematopoietic Potential of Stem Cells Isolated from Murine Skeletal Muscle ,
Proc Natl Acad Sci U S A
 96 , 14482 , 1999
Cells
Muscle hematopoietic stem cells
Enzymes
Collagenase : 0.2%
Trypsin : 0.1%
Medium
DMEM
Stem
Mouse
Mouse, 4-8 month
Gritti A, Frolichsthal-Schoeller P, Galli R, Parati E, Cova L, Pagano S, Bjornson C. and Vescovi A.
Epidermal and Fibroblast Growth Factors Behave as Mitogenic Regulators for a Single Multipotent Stem Cell-like Population from the Subventricular Region of the Adult Mouse forebrain ,
J Neurosci
 19(9) , 3287 , 1999
Cells
Neural subventricular zone
Enzymes
Trypsin : 0.13%
Hyaluronidase : 0.067%
Medium
DMEM/F12
Stem
Porcine
Porcine, 1-4 day
Park, M. , Park, J. , Kim, M. , Lee, K. , Park, H. , Yun, J. , Choi, J. , Lee, E. and Lee, S.
Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes. ,
J Assist Reprod Genet
 31 , 983-91 , 2014
Cells
Testicular
Enzymes
Hyaluronidase : 0.1%
Trypsin : 0.25%
Medium
DMEM
Stem
Porcine
Porcine, male, 8 day
Dirami G, Ravindranath N, Pursel V, Dym M.
Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM ,
Biol Reprod
 61 , 225-30 , 1999
Cells
Seminiferous epithelial cells
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.15%
Trypsin : 0.05%
Medium
DMEM/F12
Stem
Rat
Rat, adult
Duarte, S. , Momier, D. , Baque, P. , Casanova, V. , Loubat, A. , Samson, M. , Guigonis, J. , Staccini, P. , Saint-Paul, M. , De Lima, M. , Carle, G. and Pierrefite-Carle, V.
Preventive Cancer Stem Cell-Based Vaccination Reduces Liver Metastasis Development in a Rat Colon Carcinoma Syngeneic Model. ,
Stem Cells
 31 , 423-32 , 2013
Cells
Tumor
Enzymes
Medium
DMEM/F12
Stem
Rat
Rat, SD, neonatal
Liu, Z. , Wang, H. , Zhang, Y. , Zhou, J. , Lin, Q. , Wang, Y. , Duan, C. , Wu, K. and Wang, C.
Efficient Isolation of Cardiac Stem Cells from Brown Adipose. ,
J Biomed Biotechnol
 2010 , 104296 , 2010
Cells
Brown adipocytes
Enzymes
Medium
PBS
Stem
Rat
Rat, SD, newborn
Zeng Yuan-Shan, Ding Ying, Wu Li-Zhi, Guo Jia-Song, Li Hai-Biao, Wong Wai-Man, Wu Wu-Tian.
Co-transplantation of schwann cells promotes the survival and differentiation of neural stem cells transplanted into the injured spinal cord ,
Dev Neurosci
 27 , 20-6 , 2005
Cells
Neutral stem cells, Schwann cells
Enzymes
Trypsin : 0.25%
Collagenase : 0.16%
Medium
DMEM/F12
Stem
Rat
Rat, postnatal, adult
Kruger, G. , Mosher, J. , Bixby, S. , Joseph, N. , Iwashita, T. and Morrison, S.
Neural Crest Stem Cells Persist in the Adult Gut but Undergo Changes in Self-Renewal, Neuronal Subtype Potential, and Factor Responsiveness. ,
Neuron
 35 , 657 , 2002
Cells
Gut neural crest stem
Enzymes
Trypsin : 0.025%
Medium
HBSS
Stem
Rat
Rat, SD, embyronic
Bixby, S. , Kruger, G. , Mosher, J. , Joseph, N. and Morrison, S.
Cell-Intrinsic Differences Between Stem Cells from Different Regions of the Peripheral Nervous System Regulate the Generation of Neural Diversity. ,
Neuron
 35 , 643 , 2002
Cells
Sciatic nerve and gut neural crest stem
Enzymes
Trypsin : 0.005%
Medium
HBSS
Stem
Rat
Rat (also mice)
Johansson, C. , Momma, S. , Clarke, D. , Risling, M. , Lendahl, U. and Frisen, J
Identification of a Neural Stem Cell in the Adult Mammalian Central Nervous System ,
Cell
 96 , 25 , 1999
Cells
Spinal cord
Enzymes
Trypsin : 0.133%
Medium
HBSS and PIPES
Stem
Rat
Rat, SD
Morrison, S. , White, P. , Zock, C. and Anderson, D.
Prospective Identification, Isolation by Flow Cytometry, and In Vivo Self-Renewal of Multipotent Mammalian Neural Crest Stem Cells ,
Cell
 96 , 737 , 1999
Cells
Sciatic nerves
Enzymes
Trypsin : 0.025%
Medium
L-15 medium
(see reference)
Thymus
Mouse
Mouse
Phillips, Joy, Brondstetter, T. , English, C. , Lee, H. , Virts, E. and Thoman, M.
IL-7 Gene Therapy in Aging Restores Early thymopoiesis without Reversing Involution ,
J Immunol
 , 4869 , 2004
Cells
Stromal
Enzymes
Medium
RPMI 1640
Thymus
Rat
Rat, postnatal
Bonfanti, P. , Claudinot, S. , Amici, A. , Farley, A. , Blackburn, C. and Barrandon, Y.
Microenvironmental Reprogramming of Thymic Epithelial Cells to Skin Multipotent Stem Cells. ,
Nature
 466 , 978 , 2010
Cells
Thymic
Enzymes
Trypsin : 0.05%
Medium
HBSS
Thyroid/Parathyroid
Bovine
Bovine
(also porcine)
Tong, W.
The Isolation and Culture of Thyroid Cells ,
Meth Enzymol
 32 , 745 , 1974
Cells
Thyroid
Enzymes
Trypsin : 0.004%
Medium
EBSS
Thyroid/Parathyroid
Human

Human

Yi, S. , Kim, H. , Koo, B. , Lee, S. , Choi, J. and Kang, Y.
Efficient Dissociation Protocol for Generation of Single Cell Suspension from Human Thyroid Tissue for Single Cell RNA Sequencing. , Endocrinol Metab (Seoul) Vol. 37 , 698-700 , 2022
Cells
Thyroid normal and tumor
Enzymes
Trypsin : 0.125%
Collagenase Type 4 : 300 u/ml
Medium
RPMI-1640
Thyroid/Parathyroid
Human
Human
Howie, A. , Walker, S. , Akesson, B. , Arthur, J. and Beckett, G.
Thyroidal Extracellular Glutathione Peroxidase: A Potential Regulator of Thyroid-Hormone Synthesis. ,
Biochem J
 308 ( Pt 3) , 713 , 1995
Cells
Thyrocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.1%
Medium
EBSS
Thyroid/Parathyroid
Mouse
Mouse
Kitajima, S. , Kohno, S. , Kondoh, A. , Sasaki, N. , Nishimoto, Y. , Li, F. , Mohammed, M. , Muranaka, H. , Nagatani, N. , Suzuki, M. , Kido, Y. and Takahashi, C.
Undifferentiated State Induced by Rb-p53 Double Inactivation in Mouse Thyroid Neuroendocrine Cells and Embryonic Fibroblasts. ,
Stem Cells
 33 , 1657-69 , 2015
Cells
Thyroid tumor
Enzymes
Collagenase Type 1 : 300 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM
Thyroid/Parathyroid
Porcine
Porcine
(also bovine)
Tong, W.
The Isolation and Culture of Thyroid Cells ,
Meth Enzymol
 32 , 745 , 1974
Cells
Thyroid
Enzymes
Trypsin : 0.004%
Medium
EBSS
Tumor
Hamster
Hamster, 90-100 g
Gonzalez, A. , Oberley, T. , Schultz, J. , Ostrom, J. and Li, J.
In Vitro Charactrization of Estrogen Induced Syrian Hamster Renal Tumors: Comparison with an Immortalized Cell Line Derived from Diethylstilbestrol-Treated Adult Hamster Kidney ,
In Vitro Cell Dev Biol
 29A , 562 , 1993
Cells
Tumor
Enzymes
Hyaluronidase : 0.1%
Medium
Waymouth's MB
Tumor
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Tumor
Human
Human
Baldan, V. , Griffiths, R. , Hawkins, R. and Gilham, D
Efficient and Reproducible Generation of Tumour-Infiltrating Lymphocytes for Renal Cell Carcinoma ,
Br J Cancer
 112 , 1510 , 2015
Cells
Tumour infiltrating lymphocytes
Enzymes
Medium
RPMI 1640
Tumor
Human
Human
Ali, M. , Anand, S. , Tangella, K. , Ramkumar, D. and Saif, T.
Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry. ,
J Vis Exp
 , e52532 , 2015
Cells
Colon
Enzymes
Trypsin : 0.1%
Collagenase : 0.1%
Medium
HBSS
Tumor
Human
Human
Hasselbach, L. , Irtenkauf, S. , Lemke, N. , Nelson, K. , Berezovsky, A. , Carlton, E. , Transou, A. , Mikkelsen, T. and deCarvalho, A.
Optimization of High Grade Glioma Cell Culture from Surgical Specimens for Use in Clinically Relevant Animal Models and 3D Immunochemistry. ,
J Vis Exp
 , e51088 , 2014
Cells
Glioblastoma tumor
Enzymes
Trypsin : 0.025%
Collagenase Type 4 : 500 u/ml
Medium
HBSS
Tumor
Human
Human
Dobbin, Z. and Landen, C.
Isolation and Characterization of Potential Cancer Stem Cells from Solid Human Tumors--Potential Applications. ,
Curr Protoc Pharmacol
 63 , Unit 14.28. , 2013
Cells
Cancer stem
Enzymes
Trypsin : 0.25%
Collagenase : 0.05%
Hyaluronidase : 0.005%
Medium
RPMI
Tumor
Human
Human
Chou, J. , Fitzgibbon, M. , Mortales, C. , Towlerton, A. , Upton, M. , Yeung, R. , McIntosh, M. and Warren, E.
Phenotypic and Transcriptional Fidelity of Patient-Derived Colon Cancer Xenografts in Immune-Deficient Mice. ,
PLoS ONE
 8 , e79874 , 2013
Cells
Tumor
Enzymes
Medium
DMEM/F12
Tumor
Human
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Tumor
Human
Human
Quintana, E. , Shackleton, M. , Foster, H. , Fullen, D. , Sabel, M. , Johnson, T. and Morrison, S.
Phenotypic Heterogeneity Among Tumorigenic Melanoma Cells from Patients that is Reversible and Not Hierarchically Organized. ,
Cancer Cell
 18 , 510 , 2010
Cells
Tumorigenic melanoma
Enzymes
Collagenase Type 4 : 200 u/ml
Trypsin : 0.05%
Deoxyribonuclease I : 50-100 u/ml
Medium
PBS
Tumor
Human
Human
Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P.
Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. , Neuro Oncol Vol. 11 , 109 , 2009
Cells
Tumor
Enzymes
Medium
see reference
Tumor
Human
Human
Varnat, F. , Duquet, A. , Malerba, M. , Zbinden, M. , Mas, C. , Gervaz, P. and Ruiz i Altaba, A.
Human Colon Cancer Epithelial Cells Harbour Active HEDGEHOG-GLI Signalling that is Essential for Tumour Growth, Recurrence, Metastasis and Stem Cell Survival and Expansion. ,
EMBO Mol Med
 1 , 338-51 , 2009
Cells
Colon cancer
Enzymes
Collagenase Type 1 : 300 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM/F12
Tumor
Human
Human
MacLeod, R.
Rapid Monolayer Primary Cell Culture from Tissue Biopsy , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons Ltd , 3E:2.1 , 1995
Cells
Epithelial, fibroblasts
Enzymes
Trypsin : 0.25%
Medium
Ham's F-12
Tumor
Human
Human
Hague, A. and Paraskeva, C
Colon Adenocarcinoma Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12C:1.1 , 1995
Cells
Colon adenocarcinoma
Enzymes
Hyaluronidase : 100 u/ml
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.