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Tissue References Search Tool

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Brain
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Brain
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Brain
Human
Human
Sauvageot, C. , Weatherbee, J. , Kesari, S. , Winters, S. , Barnes, J. , Dellagatta, J. , Ramakrishna, N. , Stiles, C. , Kung, A. , Kieran, M. and Wen, P.
Efficacy of the HSP90 Inhibitor 17-AAG in Human Glioma Cell Lines and Tumorigenic Glioma Stem Cells. , Neuro Oncol Vol. 11 , 109 , 2009
Cells
Tumor
Enzymes
Medium
see reference
Heart
Canine
Dog, mongrel, adult
Spanier, A. and Weglicki, W.
Ca2+-Tolerant Adult Canine Myocytes: Preparation and Response to Anoxia/Acidosis ,
Am J Physiol
 243 , H448 , 1982
Cells
Myocytes
Enzymes
Hyaluronidase : 0.1%
Medium
Joklik's MEM
Heart
Guinea-Pig
Guinea-pig, male, 450-600 g
Stemmer, P. , Akera,T. , Brody, T. , Rardon, D. and Watanabe, A.
Isolation and Enrichment of Ca2+-Tolerant Myocytes for Biochemical Experiments from Guinea-Pig Heart ,
Life Sci
 44 , 1231 , 1989
Cells
Myocytes
Enzymes
Hyaluronidase : 0.02%
Medium
Krebs Henseleit bicarbonate buffer
Heart
Guinea-Pig
Guinea-pig
Bridge, J. , Spitzer, K. and Ershler, P.
Relaxation of Isolated Ventricular Cardiomyocytes by a Voltage-Dependent ,
Science
 241 , 823 , 1988
Cells
Myocytes
Enzymes
Hyaluronidase : 0.10%
Medium
Bicarbonate buffer, CF
Heart
Human
Human (n=16, age: 60 &plusmin;3 years)
Mukerjee, R. , Multani, M. , Sample, J. , Dowdy, K. , Zellner, J. , Hoover, D. and Spinale, F.
Effects of adrenomedullin on human myocyte contractile function and beta-adrenergic response ,
J Cardiovasc Pharmacol
 7 (4) , 235 , 2002
Cells
Myocardial
Enzymes
Hyaluronidase : 0.05%
Medium
Medium 199
Heart
Human
Human, fetal, 14.5 week gestation
Goldman, B. and Wurzel, J.
Effects of Subcultivation and Culture Medium on Differentiation of Human Fetal Cardiac Myocytes ,
In Vitro Cell Dev Biol
 28 , 109 , 1992
Cells
Myocytes
Enzymes
Hyaluronidase : 0.1%
Medium
HBSS with Calcium
Heart
Mouse
Mouse
Hu, D. , Yin, C. , Mohanta, S. , Weber, C. and Habenicht, A.
Prepartaion of Single Cell Suspensions from Mouse Aorta ,
Bio Protoc
 6 , 11 , 2016
Cells
Aortic
Enzymes
Collagenase Type 1 : 400 u/ml
Collagenase : 120 u/ml
Hyaluronidase : 60 u/ml
Medium
DPBS
Heart
Mouse
Mouse, 6-9 or 21-41 day
Valenzuela, D. , Han, X. , Mende, U. , Fankhauser, C. , Mashimo, H. , Huang, P. , Pfeffer, J. , Neer, E. and Fishman, M.
G alpha(o) is Necessary for Muscarinic Regulation of Ca2+ Channels in Mouse Heart ,
Proc Natl Acad Sci U S A
 94(5) , 1727 , 1997
Cells
Ventricular and atrial myocytes
Enzymes
Collagenase Type 2 : 95 u/ml
Hyaluronidase : 172.5 u/ml
Trypsin : 0.002%
Medium
DMEM/Tyrodes
Heart
Rabbit
Rabbit, New Zealand, 2.3-2.9 kg
Farkas, A. , Acsai, K. , Nagy, N. , Toth, A. , Fulop, F. , Seprenyi, G. , Birinyi, P. , Nanasi, P. , Forster, T. , Csanady, M. , Papp, J. , Varrro, A. and Farkas, A.
Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart. ,
Br J Pharmacol
 154 , 93 , 2008
Cells
Cardiomyocytes
Enzymes
Hyaluronidase : see reference
Protease : see reference
Medium
Krebs-Henseleit
Heart
Rabbit
Rabbit, New Zealand, white, male, 2-3 kg
Buxton, I. and Brunton, L.
Compartments of Cyclic AMP and Protein Kinase in Mammalian Cardiomyocytes ,
J Biol Chem
 258 (17) , 10233 , 1983
Cells
Cardiomyocytes
Enzymes
Hyaluronidase : 0.5%
Medium
Eagle's MEM
Heart
Rabbit
Rabbit, adult, 2 kg
Dani, A. , Cittadini, A. , Flamini, G. , Festuccia, G. and Terranova, T.
Preparation and Some Properties of Isolated Beating Myocytes from Adult Rabbit Heart ,
J Mol Cell Cardiol
 9 , 777 , 1977
Cells
Myocytes
Enzymes
Hyaluronidase : 0.007%
Medium
Kreb's Ringer
Heart
Rat
Rat, SD, 200-250 g
Farkas, A. , Acsai, K. , Nagy, N. , Toth, A. , Fulop, F. , Seprenyi, G. , Birinyi, P. , Nanasi, P. , Forster, T. , Csanady, M. , Papp, J. , Varrro, A. and Farkas, A.
Na(+)/Ca(2+) Exchanger Inhibition Exerts a Positive Inotropic Effect in the Rat Heart, but Fails to Influence the Contractility of the Rabbit Heart. ,
Br J Pharmacol
 154 , 93 , 2008
Cells
Cardiomyocytes
Enzymes
Hyaluronidase : see reference
Protease : see reference
Medium
Krebs-Henseleit
Heart
Rat
Rat, SD, male
Stagg Mark A, Coppen Steven R, Suzuki Ken, Varela-Carver Anabel, Lee Joon, Brand Nigel J, Fukushima Satsuki, Yacoub Magdi H, Terracciano Cesare M.
Evaluation of frequency, type, and function of gap junctions between skeletal myoblasts overexpressing connexin43 and cardiomyocytes: relevance to cell transplantation ,
FASEB J
 20 , 744-6 , 2006
Cells
Ventricular myocytes
Enzymes
Collagenase : 0.13%
Hyaluronidase : 0.06%
Medium
DMEM
Heart
Rat
Rat, SD, 300-350 g
Niederbichler Andreas D, Hoesel Laszlo M, Westfall Margaret V, Gao Hongwei, Ipaktchi Kyros R, Sun Lei, Zetoune Firas S, Su Grace L, Arbabi Saman, Sarma J Vidya, Wang StewartC, Hemmila MarkR, Ward PeterA.
An essential role for complement C5a in the pathogenesis of septic cardiac dysfunction ,
J Exp Med
 203 , 53-61 , 2006
Cells
Cardiomyocytes
Enzymes
Medium
DMEM
Heart
Rat
Rat, 50 g
Aberle II NS. and Ren J.
Experimental Assessment of the Role of Acetaldehyde in Alcoholic Cardiomyopathy ,
Biol Proced Online
 5 , 1 , 2003
Cells
Ventricular myocytes
Enzymes
Collagenase : 223 u/ml
Hyaluronidase : 0.01%
Trypsin : 0.002%
Medium
Medium 199
Heart
Rat
Rat, adult, 220-280g
Gordon Jennifer M, Dusting Gregory J, Woodman Owen L, Ritchie Rebecca H.
Cardioprotective action of CRF peptide urocortin against simulated ischemia in adult rat cardiomyocytes ,
Am J Physiol Heart Circ Physiol
 284 , H330-6 , 2003
Cells
Cardiomyocytes
Enzymes
Collagenase Type 2 : 178 u/ml
Hyaluronidase : 0.01%
Medium
Krebs-Henseleit, CF
Heart
Rat
Rat, SD, pregnant female
DeAlmeida, A. , Mustin, D. , Forman, M. , Brower, G. , Janicki, J. and Carver, W.
Effects of mast cells on the behavior of isolated heart fibroblasts: modulation of collagen remodeling and gene expression ,
J Cell Physiol
 191 , 51 , 2002
Cells
Peritoneal mast
Enzymes
Hyaluronidase : 100 u/ml
Medium
DMEM (see reference)
Heart
Rat
Rat, adult
Ren J. and Wold LE.
Measurement of Cardiac Mechanical Function in Isolated Ventricular Myocytes from Rats and Mice by Computerized Video-Based Imaging ,
Biol Proced Online
 3 , 43 , 2001
Cells
Ventricular myocytes
Enzymes
Collagenase : 223 u/ml
Hyaluronidase : 0.01%
Trypsin : 0.002%
Medium
Tyrode solution
Heart
Rat
Rat, SD, female, 200g
Westfall M V, Rust E M, Metzger J M.
Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function ,
Proc Natl Acad Sci U S A
 94 , 5444-9 , 1997
Cells
Ventricular cardiac myocytes
Enzymes
Medium
Krebs-Henseleit buffer
Heart
Rat
Rat, SD, male, 250-350 g
Kim, D. and Smith, T.W.
Temporal Variation in Contractile State And [Ca++] in Isolated Adult Rat And Guinea Pig Cardiac Myocytes , Biology of Isolated Adult Cardiac Myocytes , (Clark, Decker, Borg, eds) , Elsevier Science Publishing Co. Inc. , 370 , 1988
Cells
Myocytes, heart
Enzymes
Hyaluronidase : 0.03%
Medium
Bicarbonate-buffered medium
Heart
Rat
Rat, male/female, 200-250 g
Nag, A. and Cheng, M.
Adult Mammalian Cardiac Muscle Cells in Culture ,
Tissue Cell
 13 , 515 , 1981
Cells
Muscle
Enzymes
Hyaluronidase : 0.1%
Medium
Krebs Ringer phosphate buffer, CMF
Heart
Rat
Rat, SD, adult, male, 200-250 g
Frangakis, C. , Bahl, J. , McDaniel, H. and Bressler, R.
Tolerance to Physiological Calcium by Isolated Mycocytes from the Adult Rat Heart; An Improved Cellular Preparation ,
Life Sci
 27 , 815 , 1980
Cells
Myocytes
Enzymes
Hyaluronidase : 0.10%
Medium
Joklik MEM, CF
Heart
Rat
Rat, male, 200-300 g
Farmer, B. , Harris, R. , Jolly, W. , Hathaway, D. , Katzberg, A. , Watanabe, A. , Whitlow, A. and Besch, H.
Isolation and Characterization of Adult Rat Heart Cells ,
Arch Biochem Biophys
 179 , 545 , 1977
Cells
Myocytes
Enzymes
Hyaluronidase : 82 u/ml
Medium
Kreb's Henseleit buffer
Heart
Rat
Rat, female, albino, 200-250 g
Nag, A. , Fischman, D. , Aumont, M. and Zak, R.
Studies of Isolated Adult Rat Heart Cells: The Surface Morphology and the Influence of Extracellular Calcium Ion Concentration on Cellular Viability ,
Tissue Cell
 9 (3) , 419 , 1977
Cells
Myocytes
Enzymes
Hyaluronidase : 0.1%
Medium
Kreb's Ringers phosphate
Heart
Rat
Rat, adult, male, 150-200g
Grosso, D. , Frangakis, C. , Carlson, E. and Bressler, R.
Isolation and Characterization of Myocytes From the Adult Rat Heart ,
Prep Biochem
 7 (5) , 383 , 1977
Cells
Myocytes
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Medium
MEM CF
Heart
Rat
Rat, SD, male, 150-200 g
Pretlow II, T. , Glick, M. and Reddy, W.
Separation of Beating Cardiac Myocytes from Suspensions of Heart Cells ,
Am J Pathol
 67 (2) , 215 , 1972
Cells
Myocytes
Enzymes
Hyaluronidase : 0.05%
Medium
(see reference)
Heart
Rat
Rat, albino, male, 250 g
Berry, M. , Friend, D. and Scheuer, J.
Morphology and Metabolism of Intact Muscle Cells Isolated from Adult Rat Heart ,
Circ Res
 26 , 679 , 1970
Cells
Myocytes
Enzymes
Hyaluronidase : 0.20%
Medium
Hank's solution, CF

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.