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Tissue References Search Tool

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Mammary
Bovine
Bovine, lactating Holstein
Miranda, S. , Wang, Y. , Purdie, N. , Osborne, V. , Coomber, B. and Cant, J.
Selenomethionine Stimulates Expression of Glutathione Peroxidase 1 and 3 and Growth of Bovine Mammary Epithelial Cells in Primary Culture. ,
J Dairy Sci
 92 , 2670 , 2009
Cells
Mammary epithelial
Enzymes
Collagenase Type 3 : 400 u/ml
Hyaluronidase : 100 u/ml
Medium
HBSS
Mammary
Bovine
Bovine, heifer, 200kg
Weber M. , Purup S. , Vestergaard M. , Ellis S. , Scndergard-Andersen J. , Akers R. and Sejrsen K.
Contribution of Insulin-like Growth Factor (IGF)-I and IGF-binding Protein-3 to Mitogenic Activity in Bovine Mammary Extracts and Serum ,
J Endocrinol
 161 , 365 , 1999
Cells
Mammary epithelial
Enzymes
Medium
M-199
Mammary
Bovine
Bovine
Gibson, C. , Vega, J. , Baumrucker, C. , Oakley, C. and Welsch, C.
Establishment And Characterization Of Bovine Mammary Epithelial Cell Lines ,
In Vitro Cell Dev Biol
 27 , 585 , 1991
Cells
Epithelial
Enzymes
Hyaluronidase : 0.005%
Medium
HBSS
Mammary
Human
Human
Labarge, M. , Garbe, J. and Stampfer, M.
Processing of Human Reduction Mammoplasty and Mastectomy Tissues for Cell Culture. ,
J Vis Exp
 71 , 2013
Cells
Mammary epithelial
Enzymes
Collagenase : 200 u/ml
Hyaluronidase : 100 u/ml
Medium
DMEM/F-12
Mammary
Human
Human, female, 15-61 yrs old
Berthon, P. , Pancino, G. , Cremoux, P. , Roseto, A. , Gespach, C. and Calvo, F.
Characterization of Normal Breast Epithelial Cells in Primary Cultures: Differentiation and Growth Factor Receptors Studies ,
J Tiss Cul Meth
 28A , 716 , 1992
Cells
Epithelial
Enzymes
Hyaluronidase : 150 IU/ml
Medium
DMEM
Mammary
Human
Human
Stampfer, M. , Hallowes, R. and Hackett, A.
Growth of Normal Human Mammary Cells in Culture ,
In Vitro
 16 (5) , 415 , 1980
Cells
Epithelial
Enzymes
Hyaluronidase : 100 u/ml
Medium
DMEM/Ham's F-12
Mammary
Mouse

Mouse, female

Sun, H. , Xu, X. and Deng, C.
Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands. ,
Bio Protoc
 10 , e3530 , 2020
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F-12
Mammary
Mouse
Mouse
Liu, X. , Johnson, S. , Liu, S. , Kanojia, D. , Yue, W. , Singh, U. , Wang, Q, Wang Qi, Nie, Q. and Chen H.
Nonlinear Growth Kinetics of Breast Cancer Stem Cells: Implications for Cancer Stem Cell Targeted Therapy. ,
Sci Rep
 3 , 2473 , 2013
Cells
Mammary tumor
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.020%
Medium
DMEM/F12
Mammary
Mouse
Mouse, 10 week
Cheng, N. and Lambert, D.
Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice. ,
J Vis Exp
 54 , e2716 , 2011
Cells
Fibroblasts, carcinoma
Enzymes
Trypsin : 0.2%
Hyaluronidase : 1,000 u/ml
Medium
DMEM
Mammary
Mouse
Mouse, 12 week
Taddei, I. , Deugnier, M. , Faraldo, M. , Petit, V. , Bouvard, D. , Medina, D. , Fassler, R. , Thiery, J. and Glukhova, M.
Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells ,
Nat Cell Biol
 10 , 716-22 , 2008
Cells
Mammary epithelial
Enzymes
Collagenase : 0.3%
Hyaluronidase : 100 u/ml
Trypsin : 0.25%
Medium
see reference
Mammary
Mouse
Mouse, 11 week
Mueller, S. , Clark, J. , Myers, P. and Korach, K.
Mammary Gland Development in Adult Mice Requires Epithelial and Stromal Estrogen Receptor Alpha. ,
Endocrinology
 143 , 2357 , 2002
Cells
Epithelial
Enzymes
Collagenase Type 3 : 25 u/ml
Hyaluronidase : 0.1%
Protease XIV : 0.05%
Medium
DMEM/F12
Mammary
Mouse
Mouse
Boulanger CA, Smith GH.
Reducing mammary cancer risk through premature stem cell senescence ,
Oncogene
 20 , 2264-72 , 2001
Cells
Mammary epithelial stem
Enzymes
Hyaluronidase : 0.1%
Pronase : 1.25%
Medium
DMEM
Mammary
Mouse
Mouse (BALB/c), pregnant, 60-80 days
Ceriani, R. , Peterson, J. and Abraham, S.
Removal of Cell Surface Material by Enzymes Used to Dissociate Mammary Gland Cells ,
In Vitro
 14 , 887 , 1978
Cells
Mammary
Enzymes
Hyaluronidase : 0.1%
Medium
CMF medium
Mammary
Mouse
Mouse, BALB/cfC3H/Crgl, virgin, female
DeOme, K. , Miyamoto, M. , Osborn, R. , Guzman, R. and Lum, K.
Detection of Inaparent Nodule-transformed Cells in the Mammary Gland Tissues of Virgn Female BALB/cfC3H Mice ,
Cancer Res
 38 , 2103 , 1978
Cells
Nodule-transformed
Enzymes
Hyaluronidase : 0.1%
Medium
Medium 199
Mammary
Mouse
Mouse, CBA, virgin, 9/10 wk
Prop, F. and Wiepjes, G.
Improved method for preparation of single-cell suspensions from mammary glands of adult virgin mouse ,
Exp Cell Res
 61 , 451 , 1970
Cells
Mammary
Enzymes
Hyaluronidase : 0.1%
Medium
BSS, CMF
Mammary
Rat
Rat, Fisher 344, virgin, 80-100 day
McGrath, M. , Palmer, S. and Nandi, S.
Differential Response of Normal Rat Mammary Epithelial Cells to Mammogenic Hormones and EGF ,
J Cell Physiol
 125 , 182 , 1985
Cells
Epithelial
Enzymes
Hyaluronidase : 0.1%
Medium
Medium 199
Reproductive
Chicken

Chicken, male, 1 day

Huang, H. , Wang, M. , Hou, L. , Lin, X. , Pan, S. , Zheng, P. and Zhao, Q.
A Potential Mechanism Associated with Lead-induced Spermatogonia and Leydig Cell Toxicity and Mitigative Effect of Selenium in Chicken. , Ecotoxicol Environ Saf Vol. 209 , 111671 , 2021
Enzymes
Collagenase : 0.1%
Hyaluronidase : 0.1%
Trypsin : 0.1%
Medium
DMEM
Reproductive
Human
Human
Chen, J. and Roan, N.
Isolation and Culture of Human Endometrial Epithelial Cells and Stromal Fibroblasts. ,
Bio Protoc
 5 , 2015
Cells
Endometrial epithelial and stromal fibroblasts
Enzymes
Hyaluronidase : 62 u/ml
Medium
DMEM
Reproductive
Human
Human
Patel, M. , Ghosh, M. , Fahey, J. and Wira, C.
Uterine Epithelial Cells Specifically Induce Interferon-Stimulated Genes in Response to Polyinosinic-Polycytidylic Acid Independently of Estradiol. ,
PLoS ONE
 7 , e35654 , 2012
Cells
Uterine epithelial
Enzymes
Pancreatin : 0.34%
Hyaluronidase : 0.01%
Collagenase : 0.16%
Medium
HBSS
Reproductive
Human
Human, female
Meter, R. , Wira, C. and Fahey, J.
Secretion of Monocyte Chemotactic Protein-1 by Human Uterine Epithelium Directs Monocyte Migration in Culture. ,
Fertil Steril
 84 , 191 , 2005
Cells
Uterine epithelial
Enzymes
Pancreatin : 0.34%
Hyaluronidase : 0.01%
Collagenase : 0.16%
Medium
HBSS
Reproductive
Human
Human
Teng Yan, Xue Wu-jun, Ding Xiao-ming, Feng Xin-shun, Xiang He-li, Jiang Ya-zhuo, Tian Pu-xun.
Isolation and culture of adult Sertoli cells and their effects on the function of co-cultured allogeneic islets in vitro ,
Chin Med J (Engl)
 118 , 1857-62 , 2005
Cells
Sertoli cells
Enzymes
Medium
DMEM/F-12
Reproductive
Human
Human
Fahey John V, Wira Charles R.
Effect of menstrual status on antibacterial activity and secretory leukocyte protease inhibitor production by human uterine epithelial cells in culture ,
J Infect Dis
 185 , 1606-13 , 2002
Cells
Uterine epithelial cells
Enzymes
Pancreatin : 0.34%
Collagenase : 0.16%
Hyaluronidase : 0.16%
Medium
HBSS
Reproductive
Human
Human
Runesson E, Ivarsson K, Janson P, Brannstrom M.
Gonadotropin- and cytokine-regulated expression of the chemokine interleukin 8 in the human preovulatory follicle of the menstrual cycle ,
J Clin Endocrinol Metab
 85 , 4387-95 , 2000
Cells
Theca cells
Enzymes
Medium
PBS
Reproductive
Mouse
Mouse, 6 day
Nalbandian Angele, Dettin Luis, Dym Martin, Ravindranath Neelakanta.
Expression of vascular endothelial growth factor receptors during male germ cell differentiation in the mouse ,
Biol Reprod
 69 , 985-94 , 2003
Cells
Sertoli
Enzymes
Medium
DMEM
Reproductive
Mouse
Mouse, CF1, female
Spindle, A.
In vitro Development of One-Cell Embryos from Outbred Mice: Influence of Culture Medium Composition ,
In Vitro Cell Dev Biol
 26 , 151 , 1990
Cells
Cumulus, one-cell embryos
Enzymes
Hyaluronidase : 0.1%
Medium
PBS, CMF
Reproductive
Mouse
Mouse (BALB/cCRGL), male, 2-3 months
Turner, T. , Bern, H. , Young,P. and Cunha, G.
Serum-Free Culture of Enriched Mouse Anterior and Ventral Prostatic Epithelial Cells in Collagen Gel ,
In Vitro Cell Dev Biol
 26 , 722 , 1990
Cells
Epithelial, prostate gland
Enzymes
Hyaluronidase : 0.1%
Medium
Medium 199
Reproductive
Porcine
Porcine, 1-4 day
Park, M. , Park, J. , Kim, M. , Lee, K. , Park, H. , Yun, J. , Choi, J. , Lee, E. and Lee, S.
Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes. ,
J Assist Reprod Genet
 31 , 983-91 , 2014
Cells
Testicular
Enzymes
Hyaluronidase : 0.1%
Trypsin : 0.25%
Medium
DMEM
Reproductive
Porcine
Porcine, male, 8 day
Dirami G, Ravindranath N, Pursel V, Dym M.
Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM ,
Biol Reprod
 61 , 225-30 , 1999
Cells
Seminiferous epithelial cells
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.15%
Trypsin : 0.05%
Medium
DMEM/F12
Reproductive
Rat
Rat, SD, male, 90 days
Onoda, M. and Djakiew, D.
Pachytene Spermatocyte Protein(s) Stimulate Sertoli Cells Grown in Bicameral Chambers: Dose-Dependent Secretion of Ceruloplasmin, Sulfated Glycoprotein-1, Sulfated Glycoprotein-2, and Transferrin ,
In Vitro Cell Dev Biol
 27 , 215 , 1991
Cells
Sertoli
Enzymes
Hyaluronidase : 0.1%
Medium
DMEM
Reproductive
Rat
Rat, SD, 10 day
Hadley MA, Weeks BS, Kleinman HK, Dym M.
Laminin promotes formation of cord-like structures by Sertoli cells in vitro ,
Dev Biol
 140 , 318-27 , 1990
Cells
Sertoli cells
Enzymes
Medium
PBS
Reproductive
Rat
Rat, SD, female, pseudopregnant, 21 day
Rajendran, K. , Hwang, J. and Menon, K.
Binding, Degradation an Utilization of Plasma High Density and Low Density Lipoproteins for Progesterone Production In Cultured Rat Luteal Cells ,
Endocrinology
 112 , 1746 , 1983
Cells
Luteal, ovaries
Enzymes
Hyaluronidase : 0.1%
Medium
EBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.