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Tissue References Search Tool

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Epithelial
Human
Human
Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R. and Tseng SC
Novel enzymatic isolation of an entire viable human limbal epithelial sheet ,
Inv Ophthal Visual Sci
 44(10) , 4275 , 2003
Cells
Corneal limbal epithelial sheet
Enzymes
Medium
see reference
Epithelial
Human
Human
Smoot, D. , Sewchand, J. , Young, K. , Desbrodes, B. , Allen, C. and Naab, T.
A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells ,
Meth Cell Sci
 22 , 133 , 2000
Cells
Gastric epithelial cells
Enzymes
Medium
L-15
Epithelial
Human
Human
Halbert CL, Alexander IE, Wolgamot GM, Miller AD.
Adeno-associated virus vectors transduce primary cells much less efficiently than immortalized cells ,
J Virol
 69 , 1473-9 , 1995
Cells
Nasal polyp epithelial
Enzymes
Trypsin : 0.1%
Medium
see reference
Epithelial
Mouse
Mouse
Sun, F. , Xiao, G. and Qu, Z.
Isolation of Murine Alveolar Type II Epithelial Cells. ,
Bio-protocol
 7 , 2017
Cells
Alveolar epithelial
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse
Mathew, R. , Degenhard,t K. , Haramaty, L. , Karp, C. and White, E.
Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer. ,
Methods Enzymol
 446 , 77-106 , 2008
Cells
Epithelial
Enzymes
Collagenase : 0.025%
Medium
DMEM
Epithelial
Porcine
Porcine
De Buysscher, E. , Kennedy, J. and Mendicino, J.
Synthesis of Mucin Glycoproteins by Epithelial Cells Isolated from Swine Trachea by Specific Proteolysis ,
In Vitro
 20 , 433 , 1984
Cells
Trachea
Enzymes
Medium
HBSS
Epithelial
Rabbit
Rabbit, New Zealand white, adult
Vidrich, A. , Racindranath, R. , Farsi, K. and Targan, S.
A Method for the Rapid Establishment of Normal Adult Mammalian Colonic Epithelial Cell Cultures ,
In Vitro Cell Dev Biol
 24 (3) , 188 , 1988
Cells
Colon
Enzymes
Medium
PBS
Epithelial
Rat
Rat, embryonic
Shannon JM, Gebb SA. and Nielsen LD
Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture ,
Development
 126 , 1675 , 1999
Cells
Tracheal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Rat
Rat, 6 day old
Evans GS, Flint N, Somers AS, Eyden B. and Potten CS.
The development of a method for the preparation of rat intestinal epithelial cell primary cultures ,
J Cell Sci
 101 , 219 , 1992
Cells
Rat intestinal epithelial
Enzymes
Collagenase : 300 u/ml
Medium
DMEM
Epithelial
Rat
Rat, 6-8 day
Chang CW, Roque RS, Defoe DM. and Caldwell RB.
An improved method for isolation and culture of pigment epithelial cells from rat retina ,
Curr Eye Res
 10(11) , 1081 , 1991
Cells
Retinal pigment epithelial
Enzymes
Medium
DMEM
Eye
Human
Human
Du, Y. , Roh, D. , Funderburgh, M. , Mann, M. , Marra, K. , Rubin, J. , Li, X. and Funderburgh, J.
Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro. ,
Mol Vis
 16 , 2680 , 2010
Cells
Corneal stromal stem
Enzymes
Neutral Protease : 1.2 u/ml
Collagenase : 0.1%
Medium
DMEM
Eye
Human
Human, 18-68 yr
Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G.
A novel method of isolation, preservation, and expansion of human corneal endothelial cells ,
Inv Ophthal Visual Sci
 48 , 614-20 , 2007
Cells
Corneal endothelial
Enzymes
Medium
DMEM/F12
Eye
Human
Human
Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R. and Tseng SC
Novel enzymatic isolation of an entire viable human limbal epithelial sheet ,
Inv Ophthal Visual Sci
 44(10) , 4275 , 2003
Cells
Corneal limbal epithelial sheet
Enzymes
Medium
see reference
Eye
Mouse
Mouse, 4-8 week
Kobayashi, T. , Yoshioka, R. , Shiraishi, A. and Ohashi, Y.
New Technique for Culturing Corneal Epithelial Cells of Normal Mice. ,
Mol Vis
 15 , 1589-93 , 2009
Cells
Corneal epithelial
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Eye
Rat
Rat, 6-8 day
Chang CW, Roque RS, Defoe DM. and Caldwell RB.
An improved method for isolation and culture of pigment epithelial cells from rat retina ,
Curr Eye Res
 10(11) , 1081 , 1991
Cells
Retinal pigment epithelial
Enzymes
Medium
DMEM
Heart
Human
Human
Sharma, S. , Mishra, R. , Simpson, D. , Wehman, B. , Colletti, E. , Deshmukh, S. , Datla, S. , Balachandran, K. , Guo, Y. , Chen, L. , Siddiqui, O. , Kaushal, S. and Kaushal, S.
Cardiosphere-Derived Cells From Pediatric End-Stage Heart Failure Patients Have Enhanced Functional Activity Due to the Heat Shock Response Regulating the Secretome. ,
Stem Cells
 33 , 1213-29 , 2015
Cells
Cardiospheres
Enzymes
Medium
Iscove modified DMEM
Heart
Mouse
Mouse, 4-6 week
Xu, J. , Lee, Y. , Ran, X. , Liao, S. , Yang, J. , Au, K. , Lai, W. , Esteban, M. and Tse, H.
Generation of Induced Cardiospheres via Reprogramming of Skin Fibroblasts for Myocardial Regeneration. ,
Stem Cells
 34 , 2693-2706 , 2016
Cells
Cardiospheres
Enzymes
Medium
PBS
Heart
Mouse
Mouse
Zafiriou, M. , Noack, C. , Unsold, B. , Didie, M. , Pavlova, E. , Fischer, H. , Reichardt, H. , Bergmann, M. , El-Armouche, A. , Zimmermann, W. and Zelarayan, L.
Erythropoietin Responsive Cardiomyogenic Cells Contribute to Heart Repair Post Myocardial Infarction. ,
Stem Cells
 32 , 2480 , 2014
Cells
Cardiac progenitor
Enzymes
Medium
HBSS
Heart
Mouse
Mouse
Pratumvinit, B. , Reesukumal, K. , Janebodin, K. , Ieronimakis, N. and Reyes, M.
Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells. ,
Biomed Res Int
 2013 , 359412 , 2013
Cells
Cardiac endothelial
Enzymes
Medium
PBS
Heart
Mouse
Mouse, male, 6 week
Makino, A. , Suarez, J. , Wang, H. , Belke, D. , Scott, B. and Dillmann, W.
Thyroid Hormone Receptor-beta is Associated With Coronary Angiogenesis During Pathological Cardiac Hypertrophy. ,
Endocrinology
 150 , 2008 , 2009
Cells
Coronary endothelial cells
Enzymes
Medium
M199
Heart
Mouse
Mouse, 1 day
Potts, Malia B. , Vaughn, Allyson E. , McDonough, Holly, Patterson, Cam. and Deshmukh, Mohanish
Reduced Apaf-1 levels in cardiomyocytes engage strict regulation of apoptosis by endogenous XIAP ,
J Cell Biol
 171 , 925 , 2005
Cells
Cardiomyoctes
Enzymes
NCIS kit : per instructions
Medium
EBSS
Heart
Mouse
Mouse, neonatal 2-4 day
Bettahi, I,, Marker, C. , Roman, M. and Wickman, K.
Contribution of the Kir3.1 Subunit to the Muscarinic-gated Atrial Potassium Channel IKACh ,
J Biol Chem
 277 , 48282 , 2002
Cells
Atrial myocytes
Enzymes
NCIS kit : with modifications see reference
Medium
L-15
Heart
Mouse
Mouse, 1 day
Lader, A. , Kwiatkowski, D. and Cantiello, H.
Role of Gelsolin in the Actin Filament Regulation of Cardiac L-type Calcium Channels ,
Am J Physiol
 277 , C1277 , 1999
Cells
Cardiomyocytes
Enzymes
NCIS kit : with modifications see reference
Medium
L-15
Heart
Mouse
Mouse, 1 day
Lader, A. , Xiao, Y. , Ishikawa, Y. , Cui, Y. , Vatner, D. , Vatner, S. , Homcy, C. and Cantiello, H.
Cardiac Gsalpha Overexpression Enhances L-type Calcium Channels Through an Adenylyl Cyclase Independent Pathway ,
Proc Natl Acad Sci U S A
 95 , 9669 , 1998
Cells
Cardiomyocytes
Enzymes
NCIS kit : with modifications see reference
Medium
L-15
Heart
Rat
Rat, 1-2 day
Xie, L. , Pi, X. , Townley-Tilson, WH. , Li, N. , Wehrens, X. , Entman, M. , Taffet, G. , Mishra, A. , Peng, J. , Schisler, J. , Meissner, G. and Patterson, C.
PHD2/3-dependent Hydroxylation Tunes Cardiac Response to β-adrenergic Stress via Phospholamban. ,
J Clin Invest
 125 , 2759-71 , 2015
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
MEM
Heart
Rat
Rat, neonatal
Song, D. , Lee, K. , Ryu, J. , Jeon, H. and Kim, D.
The Molecular Interaction of Heart LIM Protein (HLP) with RyR2 and Caveolin-3 is Essential for Ca(2+)-Induced Ca(2+) Release in the Heart. ,
Biochem Biophys Res Commun
 463 , 975-81 , 2015
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
HBSS
Heart
Rat
Rat, neonatal
Sakurai, T. , Lanahan, A. , Woolls, M. , Li, N. , Tirziu, D. and Murakami, M.
Live Cell Imaging of Primary Rat Neonatal Cardiomyocytes Following Adenoviral and Lentiviral Transduction using Confocal Spinning Disk Microscopy. ,
J Vis Exp
 , e51666 , 2014
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
L-15
Heart
Rat
Rat, neonatal
Dittami, G. , Rajguru, S. , Lasher, R. , Hitchcock, R. and Rabbitt, R.
Intracellular Calcium Transients Evoked by Pulsed Infrared Radiation in Neonatal Cardiomyocytes. ,
J Physiol
 589 , 1295-306 , 2011
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
L-15
Heart
Rat
Rat, Wistar, male
Brinckmann, M. , Kaschina, E. , Altarche-Xifro, W. , Curato, C. , Timm, M. , Grzesiak, A. , Dong, J. , Kappert, K. , Kintscher, U. , Unger, T. and Li, J.
Estrogen Receptor Alpha Supports Cardiomyocytes Indirectly Through Post-Infarct Cardiac c-kit+ Cells. ,
J Mol Cell Cardiol
 47 , 66 , 2009
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
L-15
Heart
Rat
Rat, neonatal
Smith, M. , Huang, Y. and Deshmukh, M.
Skeletal Muscle Differentiation Evokes Endogenous XIAP to Restrict the Apoptotic Pathway. ,
PLoS ONE
 4 , e5097 , 2009
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
L-15
Heart
Rat
Rat, SD, 1-2 day
Kim, M. , Oh, J. , Sakata, S. , Liang, I. , Park, W. , Hajjar, R. and Lebeche, D.
Role of Resistin in Cardiac Contractility and Hypertrophy. ,
J Mol Cell Cardiol
 45 , 270 , 2008
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
L-15
Heart
Rat
Rat, neonatal
Robinet, A. , Millart, H. , Oszust, F. , Hornebeck, W. and Bellon, G.
Binding of Elastin Peptides to S-Gal Protects the Heart Against Ischemia/Reperfusion Injury by Triggering the RISK Pathway. ,
FASEB J
 21 , 1968 , 2007
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
L-15
Heart
Rat
Rat, Wistar, neonatal
Butler, T. , Au, C. , Yang, B. , Egan, J. , Tan, Y. , Hardeman, E. , North, K. , Verkman, A. and Winlaw, D.
Cardiac Aquaporin Expression in Humans, Rats, and Mice. ,
Am J Physiol Heart Circ Physiol
 291 , H705 , 2006
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
L-15
Heart
Rat
Rat, 1-2 day
Pedram, A. , Razandi, M. , Aitkenhead, M. and Levin, E
Estrogen Inhibits Cardiomyocyte Hypertrophy in vitro. Antagonism of Calcineurin-related Hypertrophy through Induction of MCIP1 ,
J Biol Chem
 280 , 26339 , 2005
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
DMEM/F-12
Heart
Rat
Rat, Wistar, 1-3 day
Castillo, A. , Ruzmetov, N. , Harvey, K. , Stillwell, W. , Zaloga, G. and Siddiqui, R.
Docosahexaenoic Acid Inhibits Protein Kinase C Translocation/Activation and Cardiac Hypertrophy in Rat Cardiomyocytes. ,
J Mol Genet Med
 1 , 18 , 2005
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
L-15
Heart
Rat
Rat, SD, 1-2 day
Muller-Bore, B. , Cascio, W. , Anderson, P. , Snowwaert, J. , Frye, J. , Desai, N. , Esch, G. , Brackham, J. , Bagnell, C. , Coleman, W. , Grisham, J. and Malouf, N.
Adult-derived Liver Stem Cells Acquire a Cardiomyocyte Structural and Functional Phenotype ex vivo ,
Am J Pathol
 165 , 135 , 2004
Cells
Cardiomyocytes
Enzymes
NCIS kit : per instructions
Medium
L-15

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.