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Tissue References Search Tool

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Reproductive
Mouse
Mouse, BALB/c
Bigsby, R. , Cooke, P. and Cunha, G.
A Simple Efficient Method For Separating Murine Uterine Epithelial and Mesenchymal Cells ,
Am J Physiol
 251 , E630 , 1986
Cells
Epithelial
Mesencymal
Enzymes
Trypsin : 1%
Medium
DMEM
Reproductive
Mouse
Mouse, BALB/cCrg, 40 days old
Cooke, P. , Uchima, F. , Fujii, D. , Bern, H. and Cunha, G.
Restoration of Normal Morphology and Estrogen Responsiveness in Cultured Vaginal and Uterine Epithelia Transplanted with Stroma ,
Proc Natl Acad Sci U S A
 83 , 2109 , 1986
Cells
Epithelial
Enzymes
Trypsin : 1%
Medium
Medium 199
Reproductive
Porcine
Porcine, 1-4 day
Park, M. , Park, J. , Kim, M. , Lee, K. , Park, H. , Yun, J. , Choi, J. , Lee, E. and Lee, S.
Development of a High-Yield Technique to Isolate Spermatogonial Stem Cells From Porcine Testes. ,
J Assist Reprod Genet
 31 , 983-91 , 2014
Cells
Testicular
Enzymes
Hyaluronidase : 0.1%
Trypsin : 0.25%
Medium
DMEM
Reproductive
Porcine
Porcine, male, 8 day
Dirami G, Ravindranath N, Pursel V, Dym M.
Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM ,
Biol Reprod
 61 , 225-30 , 1999
Cells
Seminiferous epithelial cells
Enzymes
Collagenase : 0.15%
Hyaluronidase : 0.15%
Trypsin : 0.05%
Medium
DMEM/F12
Reproductive
Porcine
Porcine, 3-4 week
Mather, J. , Saez, J. and Haour, F.
Regulaton of Gonadotropin Receptors and Steroidogenesis in Cultured Porcine Leydig Cells ,
Endocrinology
 110 , 933 , 1982
Cells
Leydig
Testis
Enzymes
Trypsin : 0.0003%
Medium
Lebovitz L-15 Medium
Reproductive
Rabbit
Rabbit, New Zealand, 3 month
Kubota, H. , Wu, X. , Goodyear, S. , Avarbock, M. and Brinster, R.
Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties. ,
FASEB J
 25 , 2604 , 2011
Cells
Testicular germ
Enzymes
Medium
HBSS
Reproductive
Rabbit
Rabbit, New Zealand, 3 month
Kubota, H. , Wu, X. , Goodyear, S. , Avarbock, M. and Brinster, R.
Glial Cell Line-Derived Neurotrophic Factor and Endothelial Cells Promote Self-Renewal of Rabbit Germ Cells with Spermatogonial Stem Cell Properties. ,
FASEB J
 25 , 2604 , 2011
Cells
Testicular germ
Enzymes
Medium
HBSS
Reproductive
Rabbit
Rabbit, New Zealand white, mature, 3-4 Kg
Boulet, A. and Fortier, M.
Preparation and Characterization of Rabbit Myometrial Cells in Primary Culture: Influence of Estradiol and Progesterone Treatment ,
In Vitro Cell Dev Biol
 23 , 93 , 1987
Cells
Myometrial
Enzymes
Trypsin : 0.02%, 0.03%, 0.0375%
Medium
HBSS
Reproductive
Rabbit
Rabbit, New Zealand white estrous, female, 4-5 months
Nicosia, S. , Johnson, J. and Streibel, E.
Isolation and Ultrastructure of Rabbit Ovarian Mesothelium(Surface Epithelium) ,
Int J Gynecol Pathol
 3 , 348 , 1984
Cells
Mesothelial and surface epithelial
Ovaries
Enzymes
Trypsin : 0.125%-0.5%
Medium
Medium 199
Reproductive
Rat
Rat, SD, adult, male, 8 - 10 week
Abou-Haila, A. and Tulsiani, D.
Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization ,
Biol Proced Online
 3 (1) , 35 , 2001
Cells
Seminiferous tubules
Enzymes
Trypsin : 0.05%
Medium
Krebs-Ringer bicarbonate buffer
(see reference)
Reproductive
Rat
Rat, Han-Wistar, adult, male, 250 g
Leckie, C. , Welberg, L. and Seckl, J.
11B-Hydroxysteroid Dehydrogenase is a Predominant Reductase in Intact Rat Leydig Cells ,
J Endocrinol
 159 , 233 , 1998
Cells
Testes
Enzymes
Trypsin : 0.1%
Medium
Medium 199 w/ Hank's salts
Reproductive
Rat
Rat, SD, male
Abayasekara, D. , Kurlak, L. , Band, A. , Sullivan, M. and Cooke, B.
Effect of Cell Purity, Cell Concentration, and Incubation Conditions on Rat Testis Leydig Cell Steroidogenesis ,
In Vitro Cell Dev Biol
 27 , 253 , 1991
Cells
Leydig
Enzymes
Trypsin : 0.02%
Medium
DMEM
Reproductive
Rat
Rat, neonatal
Branham, W. , Lyn-Cook, B. , Andrews, A. , McDaniel, M. and Sheehan, D.
Growth of Neonatal Rat Uterine Luminal Epithelium on Extracellular Matrix ,
In Vitro Cell Dev Biol
 27 , 442 , 1991
Cells
Uterine
Enzymes
Trypsin : 1%
Medium
HBSS
Reproductive
Rat
Rat, Wistar, adult
Pampfer, S. , Vanderheyden, I. , Michiels, B. and DeHertogh, R.
Co-Culture of Two-Cell Rat Embryos on Cell Monolayers ,
In Vitro Cell Dev Biol
 26 , 944 , 1990
Cells
Uterine
Enzymes
Trypsin : 0.5%
Medium
PBS
Reproductive
Rat
Rat, SD, female
Glasser, S. , Julian, J. , Decker, G. , Tang, J. and Carson, D.
Development of Morphological and Functional Polarity in Primary Cultures of Immature Rat Uterine Epithelial Cells ,
J Cell Biol
 107 (6) , 2409 , 1988
Cells
Luminal epithelial
Enzymes
Trypsin : 0.5%
Medium
HBSS
Reproductive
Rat
Rat, male, 20 day
Skinner, M. , Fetterolf, P. and Anthony, C.
Purification of a Paracrine Factor, P-Mod-S,Produced by Testicular Peritubular Cells That Modulates Sertoli Cell Function ,
J Biol Chem
 263 , 2884 , 1988
Cells
Sertoli
Enzymes
Trypsin : 0.15%
Medium
(see reference)
Reproductive
Rat
Rat, Wistar, virgin, female, 250 g
Conti, C. and Tasat, D.
Regulation of Cultured Rat Vaginal Epithelial Cells By 17B-Estradiol and Progesterone ,
J Steroid Biochem
 24 (3) , 747 , 1986
Cells
Vaginal epithelial
Enzymes
Trypsin : 0.5%
Medium
PBS
Reproductive
Rat
Rat, SD, male, 10 day
Hadley, M. , Byers, S. , Suarez-Quian, C. , Kleinman, H. and Dym, M.
Extracellular Matrix Regulates Seratoli Cell Differentiation, Testicular Cord Formation and germ cell development in vitro ,
J Cell Biol
 101 , 1511 , 1985
Cells
Sertoli
Enzymes
Trypsin : 0.025%
Medium
DMEM
Skin
Frog
Frog
Nishikawa, A. , Shimizu-Nishikawa, K. and Miler, L.
Isolation, characterization, and in vitro culture of larval and adult epidermal cells of the frog Xenopus laevis ,
In Vitro Cell Dev Biol
 26 , 1128 , 1990
Cells
Epidermal
Enzymes
Trypsin : 0.18%
Medium
Barth's solution, CMF
Skin
Human
Human
Botting, Ra. , Bertram, K. , Baharlou, H. , Sandgren, K. , Fletcher, J. , Rhodes, J. , Rana, H. , Plasto, T. , Wang, X. , Lim, J. , Barnouti, L. , Kohout, M. , Papadopoulos, T. , Merten, S. , Olbourne, N. and Cunningham, A.
Phenotypic and Functional Consequences of Different Isolation Protocols on Skin Mononuclear Phagocytes. ,
J Leukoc Biol
 101 , 1393-1403 , 2017
Cells
Mononuclear phagocytes
Enzymes
Neutral Protease : 1 u/ml
Collagenase : 200 u/ml
Trypsin : 0.5%
Medium
RPMI-1640
Skin
Human
Human, adult
Nagel, S. , Rohr, F. , Weber, C. , Kier, J. , Siemers, F. , Kruse, C. , Danner, S. , Brandenburger, M. and Matthiessen, A.
Multipotent Nestin-Positive Stem Cells Reside in the Stroma of Human Eccrine and Apocrine Sweat Glands and can be Propagated Robustly In Vitro. ,
PLoS ONE
 8 , e78365 , 2013
Cells
Epidermal stem cells
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 2.5%
Medium
DMEM
Skin
Human
Human, female, 18-66 yr
Huschtscha, L. , Napier, C. , Noble, J. , Bower, K. , Au, A. , Campbell, H. , Braithwaite, A. and Reddel, R.
Enhanced Isolation of Fibroblasts From Human Skin Explants. ,
Biotechniques
 53 , 239 , 2012
Cells
Fibroblasts
Enzymes
Trypsin : 0.2%
Medium
DMEM
Skin
Human
Human
Yu Hong, Fang Dong, Kumar Suresh M, Li Ling, Nguyen Thiennga K, Acs Geza, Herlyn Meenhard, Xu Xiaowei.
Isolation of a novel population of multipotent adult stem cells from human hair follicles ,
Am J Pathol
 168 , 1879-88 , 2006
Cells
Hair follicular epithelial
Enzymes
Trypsin : 0.25%
Medium
DMEM/F-12
Skin
Human
Human
Clark, R. , Chong, B. , Mirchandani, N. , Yamanaka, K. , Murphy, G. , Dowgiert, R. and Kupper, T.
A Novel Method for the Isolation of Skin Resident T cells from Normal and Diseased Human Skin. ,
J Invest Dermatol
 126 , 1059 , 2006
Cells
fibroblasts
Enzymes
Collagenase : 0.5%
Trypsin : 0.25%
Medium
DMEM/F12
Skin
Human
Human
Tuan Tai-Lan, Wu Huayang, Huang Eunice Y, Chong Sheree S N, Laug Walter, Messadi Diana, Kelly Paul, Le Anh.
Increased plasminogen activator inhibitor-1 in keloid fibroblasts may account for their elevated collagen accumulation in fibrin gel cultures ,
Am J Pathol
 162 , 1579-89 , 2003
Cells
Fibroblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human
Hybbinette S, Bostrom M, Lindberg K.
Enzymatic dissociation of keratinocytes from human skin biopsies for in vitro cell propagation ,
Exp Dermatol
 8 , 30-8 , 1999
Cells
Keratinocytes
Enzymes
Trypsin : 0.17%
Thermolysin : 0.05%
Medium
see reference
Skin
Human
Human
Judd, D. , Battista, P. and Behm, D.
Culture of Human Keratinocytes in Defined Serum-Free Medium ,
Focus
 19 (1) , 2 , 1997
Cells
Keratinocytes
Enzymes
Trypsin : 0.05%
Medium
Dulbecco's PBS
Skin
Human
Human
Harley, C. and Sherwood, S.
, Methods in Molecular Biology, Basic Cell Culture Protocols, 2nd ed. Vol. 75 , Pollard, J., and Walker, J. , Humana Press , 23 , 1997
Cells
Epidermis plus dermis
Abdomen or inner forearm
Enzymes
Trypsin : 0.125%
Medium
DMEM, M199
Skin
Human
Human
Regnier, M.
Culture of Human Karatinocytes , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons Ltd , 11B:4.1 , 1995
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human, adult, female, breast skin
Hirel, B. , Chesne, C. , Pailgheret, J. and Guillouzo, A.
Expression of Differentiation Markers in Human Adult Keratinocytes Cultured in Submerged Conditions ,
In Vitro Cell Dev Biol
 30A , 372 , 1994
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
PBS
Skin
Human
Human, neonatal foreskin
Hansbrough, J. , Cooper, M. , Cohen, R. , Spielvogel, R. , Greenleaf, G. , Bartel, R. and Naughton, G.
Evaluation of a Biodegradable Matrix Containing Cultured Human Fibroblasts as a Dermal Replacement Beneath Meshed Skin Grafts on Athymic Mice ,
Surgery
 111 (1) , 438 , 1992
Cells
Fibroblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human
Limat, A. , Hunziker, T. , Boillat, C. , Noser, F. and Wiesmann, U.
Postmitotic Human Dermal Fibroblasts Preserve Intact Feeder Properties for Epithelial Cell Growth After Long-Term Cryopreservation ,
In Vitro Cell Dev Biol
 26 , 709 , 1990
Cells
Fibroblasts
Enzymes
Trypsin : 0.25%
Medium
CMF solution
Skin
Human
Human
Peacocke, M. , Yaar, M. , Mansur, C. , Chao, M. and Gilchrest, B.
Induction of Nerve Growth Factor Receptors on Cultured Human Melanocytes ,
Proc Natl Acad Sci U S A
 85 , 5282 , 1988
Cells
Melanocytes, skin/ foreskin
Enzymes
Trypsin : 0.25%
Medium
PBS
Skin
Human
Human
Dover, R. and Potten, C.
Cell Cycle Kinetics of Cultured Human Epidermal Keratinocytes ,
J Invest Dermatol
 80 , 423 , 1983
Cells
Keratinocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM
Skin
Human
Human, 18-30 years, male, female
McCoy, B. , Galdun, J. and Cohen, I.
Effects of Density and Cellular Aging On Collagen Synthesis and Growth Kinetics in Keloid and Normal Skin Fibroblasts ,
In Vitro
 18 (1) , 79 , 1982
Cells
Fibroblasts
Enzymes
Trypsin : 0.1%
Medium
HBSS
Skin
Human
Human, adult
Alitalo K, Kuismanen E, Myllyla R, Kiistala U, Asko-Seljavaara S, Vaheri A.
Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells ,
J Cell Biol
 94 , 497-505 , 1982
Cells
Epidermal keratinocytes
Enzymes
Trypsin : 0.25%
Collagenase : 0.2%
Medium
Eagle's MEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.