Tissue References Search Tool

Endothelial

Bovine

Yu, M. , McAndrew, R. , Al-Saghir, R. , Maier, K. , Medhora, M. , Roman, R. and Jacobs, E.

Nitric Oxide Contributes to 20-HETE-Induced Relaxation of Pulmonary Arteries. ,
J Appl Physiol
93 , 1391 , 2002

Cells

Pulmonary artery endothelial and smooth muscle cells

Enzymes

Collagenase : 0.04-0.05%

Soybean Trypsin Inhibitor : 0.04%

Medium

RPMI-1640

Endothelial

Human

Human, 18-68 yr

Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G.

A novel method of isolation, preservation, and expansion of human corneal endothelial cells ,
Inv Ophthal Visual Sci
48 , 614-20 , 2007

Cells

Corneal endothelial

Enzymes

Collagenase : 0.2%

Neutral Protease : 1.0%

Medium

DMEM/F12

Endothelial

Human

Muller AM, Hermanns MI, Skrzynski C, Nesslinger M, Muller KM. and Kirkpatrick CJ.

Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro ,
Exp Mol Pathol
72 , 221 , 2002

Cells

Pulmonary vascular endothelial cells

Enzymes

Neutral Protease : 1.18 u/ml

Elastase : 10 u/ml

Medium

M199

Endothelial

Human

Farber HW, Antonov AS, Romanov YA, Smirnov VN, Scarfo LM, Beer DJ.

Cytokine secretion by human aortic endothelial cells is related to degree of atherosclerosis ,
Am J Physiol
262 , H1088-95 , 1992

Cells

Vascular endothelial

Enzymes

Neutral Protease : 0.15%

Trypsin : 0.25%

Medium

M-199

Endothelial

Human

Sherer, G. , Fitzharris, T. , Faulk, W. and LeRoy, E.

Cultivation of Microvascular Endothelial Cells from Human Preputial Skin ,
In Vitro
16 , 675 , 1980

Cells

Microvascular endothelial

Neonatal foreskins

Enzymes

Neutral Protease : at 1000 u/ml

Medium

Konigsberg's modification of HBSS

(See Reference)

Endothelial

Mouse

Luo, S. , Truong, A. and Makino, A.

Isolation of Mouse Coronary Endothelial Cells. ,
J Vis Exp
, 2016

Cells

Coronary endothelial

Enzymes

Collagenase Type 2 : 0.1%

Neutral Protease : 0.6 u/ml

Medium

Kreb's

Endothelial

Mouse

Xiao, L. , McCann, J. and Dudley, A.

Isolation and Culture Expansion of Tumor-specific Endothelial Cells. ,
J Vis Exp
, e53072 , 2015

Cells

Endothelial

Enzymes

Collagenase Type 2 : 0.1%

Neutral Protease : 0.25 u/ml

Deoxyribonuclease I : 0.0075%

Medium

DMEM

Endothelial

Mouse

Kochukov, M. , Balasubramanian, A. , Abramowitz, J. , Birnbaumer, L. and Marrelli, S.

Activation of Endothelial Transient Receptor Potential C3 Channel is Required for Small Conductance Calcium-Activated Potassium Channel Activation and Sustained Endothelial Hyperpolarization and Vasodilation of Cerebral Artery. ,
J Am Heart Assoc
3 , 2014

Cells

Cerebral artery endothelial

Enzymes

Neutral Protease : 4 u/ml

Elastase : 1 u/ml

Collagenase Type 1 : 120 u/ml

Medium

DMEM

Endothelial

Mouse

Mouse, neonatal

Cha, S. , Talavera, D. , Demir, E. , Nath, A. and Sierra-Honigmann, M.

A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. ,
Microvasc Res
70 , 198 , 2005

Cells

Microvascular endothelial

Enzymes

Neutral Protease : 0.005%

Collagenase Type 1 : 4%

Medium

DMEM

Endothelial

Porcine

Porcine, 6 month

Balaoing, L. , Post, A. , Lin, A. , Tseng, H. , Moake, J. and Grande-Allen, K.

Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation. ,
PLoS ONE
10 , e0130749 , 2015

Cells

Valvular endothelial

Enzymes

Neutral Protease : 2 u/ml

Collagenase Type 2 : 60 u/ml

Medium

PBS

Endothelial

Rat

Rat, SD, male, 250-300 g

Schwertschlag, U.S. and Whorton, A.R.

Platelet-Activating Factor-Induced Homologous and Heterologous Desensitization In Cultured Vascular Smooth Muscle Cells ,
J Biol Chem
263 , 13791 , 1988

Cells

Smooth muscle, aorta

Enzymes

Soybean Trypsin Inhibitor : 0.25%

Medium

HBSS with 0.2 mM Ca++

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Tissue References Search Tool

Tissue Tables (references, grouped by tissue type and species)