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Tissue References Search Tool

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Endothelial
Bovine
Calf
Vender, R.
Role of Endothelial Cells in the Proliferative Response of Cultured Pulmonary Vascular Smooth Muscle Cells to Reduced Oxygen Tension ,
In Vitro Cell Dev Biol
 28A , 403 , 1992
Cells
Endothelial
Enzymes
Trypsin : 0.25%
Medium
HEPES
Endothelial
Bovine
Bovine
DeNucci, G. , Gryglewski, R.J. , Warner, T.D. and Vane, J.R.
Receptor-Mediated Release of Endothelium-Derived Relaxing Factor and Prostacyclin From Bovine Aortic Endothelial Cells Is Coupled ,
Proc Natl Acad Sci U S A
 85 , 2334 , 1988
Cells
Endothelial
Aortic
Enzymes
Trypsin : 0.05%
Medium
Krebs Ringer solution
Endothelial
Bovine
Bovine
Scott, D. , Murray, J. and Barnes, M.
Investigation of the Attachment of Bovine Corneal Endothelial Cells ,
Exp Cell Res
 144 , 472 , 1983
Cells
Endothelial
Corneal
Enzymes
Trypsin : 0.2%
Medium
PBS: DMEM
Endothelial
Bovine
Bovine
Robinson, J. and Gospodarowicz, D.
Glycosaminoglycans Synthesized by Cultured Bovine Corneal Endothelial Cells ,
J Cell Physiol
 117 , 368 , 1983
Cells
Endothelial, Corneal
Enzymes
Trypsin : 0.05%
Medium
0.01M Phosphate buffer with 0.02% EDTA
0.9% NaCl (See Reference)
Endothelial
Canine
Dog, mongrel, adult
Ford, J. , Burkel, W. and Kahn, R.
Isolation of Adult Canine Venous Endothelium for Tissue Culture ,
In Vitro
 17 , 44 , 1981
Cells
Endothelium
Jugular vein
Enzymes
Trypsin : 0.1%
Medium
Earle's PBS, CMF
Endothelial
Human
Human, 18-68 yr
Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G.
A novel method of isolation, preservation, and expansion of human corneal endothelial cells ,
Inv Ophthal Visual Sci
 48 , 614-20 , 2007
Cells
Corneal endothelial
Enzymes
Medium
DMEM/F12
Endothelial
Human
Human
Muller AM, Hermanns MI, Skrzynski C, Nesslinger M, Muller KM. and Kirkpatrick CJ.
Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro ,
Exp Mol Pathol
 72 , 221 , 2002
Cells
Pulmonary vascular endothelial cells
Enzymes
Neutral Protease : 1.18 u/ml
Elastase : 10 u/ml
Medium
M199
Endothelial
Human
Human
Goolcharran, C. , Cleland, J. , Keck, R. , Jones, A. and Borchardt, R.
Comparsion of the Rates of Deamidation, Diketopiperazine Formation and Oxidation in Recombinant Human Vascular Endothelial Growth Factor and Model Peptides ,
AAPS PharmSci
 2 (1) , 5 , 2000
Cells
Endothelial
Enzymes
Trypsin : 2%
Medium
PBS
Endothelial
Human
Human
Wojta J, Gallicchio M, Zoellner H, Filonzi E, Hamilton J, McGrath K.
Interleukin-4 stimulates expression of urokinase-type-plasminogen activator in cultured human foreskin microvascular endothelial cells ,
Blood
 81 , 3285-92 , 1993
Cells
Foreskin microvascular endothelial
Enzymes
Trypsin : 0.3%
Medium
HBSS
Endothelial
Human
Human
Farber HW, Antonov AS, Romanov YA, Smirnov VN, Scarfo LM, Beer DJ.
Cytokine secretion by human aortic endothelial cells is related to degree of atherosclerosis ,
Am J Physiol
 262 , H1088-95 , 1992
Cells
Vascular endothelial
Enzymes
Trypsin : 0.25%
Medium
M-199
Endothelial
Human
Human
Lee, K, Lawley, T, Xu, Y. and Swerlick, R.
VCAM-1-, ELAM-1-, and ICAM-1-Independent Adhesion of Melanoma Cells to Cultured Human Dermal Microvascular Endothelial Cells ,
J Invest Dermatol
 98 , 79 , 1992
Cells
Endothelial
Enzymes
Trypsin : 0.3.%
Medium
HBSS
(see reference)
Endothelial
Human
Human
Kubota, Y. , Kleinman, H. , Martin, G. and Lawley, T.
Role of Laminin and Basement Membrane in Morphological Differentiation of Human Endothelial Cells into Capillary-like Structures ,
J Cell Biol
 107 , 1589 , 1988
Cells
Endothelial/HUVEC
Foreskin & umbilical cord
Enzymes
Trypsin : 0.3%
Medium
HBSS/PBS, Medium 199
(see reference)
Endothelial
Human
Human
Marks, R.M. , Czerniecki, M. and Penny, R.
Human Dermal Microvascular Endothelial Cells: An Improved Method for Tissue Culture and Description of Some Singular Properties in Culture ,
In Vitro Cell Dev Biol
 21 , 627 , 1985
Cells
Endothelial
Dermal
Enzymes
Trypsin : 0.3%
Medium
PBS
Endothelial
Human
Human
Sherer, G. , Fitzharris, T. , Faulk, W. and LeRoy, E.
Cultivation of Microvascular Endothelial Cells from Human Preputial Skin ,
In Vitro
 16 , 675 , 1980
Cells
Microvascular endothelial
Neonatal foreskins
Enzymes
Neutral Protease : at 1000 u/ml
Medium
Konigsberg's modification of HBSS
(See Reference)
Endothelial
Human
Human
Jaffe, E.A. , Minick, C.R. , Adelman, B. , Becker, C.G. and Nachman, R.
Synthesis of Basement Membrane Collagen By Cultured Human Endothelial Cells ,
J Exp Med
 144 , 209 , 1976
Cells
Umbilical vein
Enzymes
Trypsin : 100 µg/ml
Medium
Tris-HCl,0.2 M
Endothelial
Mouse
Mouse
Luo, S. , Truong, A. and Makino, A.
Isolation of Mouse Coronary Endothelial Cells. ,
J Vis Exp
 , 2016
Cells
Coronary endothelial
Enzymes
Medium
Kreb's
Endothelial
Mouse
Mouse
Xiao, L. , McCann, J. and Dudley, A.
Isolation and Culture Expansion of Tumor-specific Endothelial Cells. ,
J Vis Exp
 , e53072 , 2015
Cells
Endothelial
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse
Kochukov, M. , Balasubramanian, A. , Abramowitz, J. , Birnbaumer, L. and Marrelli, S.
Activation of Endothelial Transient Receptor Potential C3 Channel is Required for Small Conductance Calcium-Activated Potassium Channel Activation and Sustained Endothelial Hyperpolarization and Vasodilation of Cerebral Artery. ,
J Am Heart Assoc
 3 , 2014
Cells
Cerebral artery endothelial
Enzymes
Neutral Protease : 4 u/ml
Elastase : 1 u/ml
Collagenase Type 1 : 120 u/ml
Medium
DMEM
Endothelial
Mouse
Mouse, neonatal
Cha, S. , Talavera, D. , Demir, E. , Nath, A. and Sierra-Honigmann, M.
A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. ,
Microvasc Res
 70 , 198 , 2005
Cells
Microvascular endothelial
Enzymes
Medium
DMEM
Endothelial
Porcine
Porcine, 6 month
Balaoing, L. , Post, A. , Lin, A. , Tseng, H. , Moake, J. and Grande-Allen, K.
Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation. ,
PLoS ONE
 10 , e0130749 , 2015
Cells
Valvular endothelial
Enzymes
Medium
PBS
Endothelial
Porcine
Porcine
Shasby, S.
Endothelial Cells Grown On Permeable Membrane Supports ,
J Tiss Cul Meth
 14 , 247 , 1992
Cells
Endothelial
Enzymes
Trypsin : 0.25%
Medium
Medium 199
Endothelial
Porcine
Porcine
Vischer, P. and Buddecke, E.
Alteration of Glycosytransferase Activities during Proliferation of Cultivated Arterial Endothelial Cells and Smooth Muscle Cells ,
Exp Cell Res
 158 , 15 , 1985
Cells
Endothelial
Enzymes
Trypsin : 0.05%
Medium
DMEM
Endothelial
Porcine
Porcine, 30-40 kg
Merrilees, M.J. and Scott, L.
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
 39 , 147 , 1981
Cells
Endothelial
Thoracic aorta
Enzymes
Trypsin : 0.05%
Medium
Medium 199 and 0.01M EDTA
Endothelial
Porcine
Porcine, 60-100 days
Coulson, W.F.
The Effect Of Proteolytic Enzymes on the Tensile Strength of Whole Aorta and Isolated Aortic Elastin ,
Biochim Biophys Acta
 237 , 378 , 1971
Cells
Aorta
Enzymes
Trypsin : 0.1%
Medium
Phosphate buffer
(see reference)
Endothelial
Rat
Rat, 300 G, and pig, 30-40 Kg
Merrilees, M.J. and Scott, L.
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
 39 , 147 , 1981
Cells
Endothelial
Thoracic aorta
Enzymes
Trypsin : 0.05%
Medium
Medium 199 and 0.01M EDTA
Endothelial
Rat
Rat, Wistar, adult, 170 g
Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.
Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
 129 , 261 , 1979
Cells
Endothelial
Enzymes
Trypsin : 0.5%
Medium
BSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.