Tissue References Search Tool

Human

Rustenhoven, J. , Park Thomas, I. , Schweder, P. , Scotter, J. , Correia, J. , Smith, A. , Gibbons, H. , Oldfield, R. , Bergin, P. , Mee, E. , Faull, R. , Curtis, M. , Scott, G. and Dragunow, M.

Isolation of Highly Enriched Primary Human Microglia for Functional Studies. ,
Sci Rep
6 , 19371 , 2016

Cells

Microglia

Enzymes

Neutral Protease : 10 u/ml

Papain : 2.5 u/ml

Medium

Hibernate A

Brain

Human

Human, 25-81 yr

Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,

A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
17 , 30 , 2016

Cells

Brain tumor

Enzymes

Neutral Protease : 0.11 u/ml

Collagenase Type 4 : 0.05%

Hyaluronidase : 1,000 u/ml

Deoxyribonuclease I : 5 u/ml

Medium

HBSS

Brain

Human

Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.

Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
13 , 70-83 , 2011

Cells

Brain tumor

Enzymes

Collagenase Type 1 : 0.04%

Hyaluronidase : 0.01%

Deoxyribonuclease I : 0.02%

Neutral Protease : 0.008%

Medium

DMEM/F12