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Tissue References Search Tool

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Brain
Bovine
Bovine
Kanda, T. , Yoshino, H. , Ariga, T. , Yamawaki, M. and Yu, R.
Glycosphingolipid Antigens in Cultured Microvasular Bovine Brain Endothelial Cells: Sulfoglucuronosyl Paragloboside as a Target of Monoclonal IgM in Demyelinative Neuropathy ,
J Cell Biol
 126 (1) , 235 , 1994
Cells
Microvascular endothelial
Enzymes
Neutral Protease : 0.005%
Medium
Medium 199
Brain
Bovine
Bovine
Miller, D. , Audus, K. and Borchardt, R.
Application of Cultured Endothelial Cells of the Brain Microvasculature in the Study of the Blood-Brain Barrier ,
J Tiss Cul Meth
 14 , 217 , 1992
Cells
Endothelial
Enzymes
Neutral Protease : 0.125%
Medium
MEM
Brain
Bovine
Bovine
Audus, K. and Borchardt, R.
Characterization of an In Vitro Blood-Brain Barrier Model System for Studying Drug Transport and Metabolism ,
Pharm Res
 3 (2) , 81 , 1986
Cells
Endothelial
Enzymes
Medium
MEM
Brain
Human
Human
Rustenhoven, J. , Park Thomas, I. , Schweder, P. , Scotter, J. , Correia, J. , Smith, A. , Gibbons, H. , Oldfield, R. , Bergin, P. , Mee, E. , Faull, R. , Curtis, M. , Scott, G. and Dragunow, M.
Isolation of Highly Enriched Primary Human Microglia for Functional Studies. ,
Sci Rep
 6 , 19371 , 2016
Cells
Microglia
Enzymes
Neutral Protease : 10 u/ml
Papain : 2.5 u/ml
Medium
Hibernate A
Brain
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Brain
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Brain
Mouse
Adult and neonatal
Calvo, Belen, Rubio, F. , Fernandez, M. and Tranque, P.
Dissociation of Neonatal and Adult Mice Brain for Simultaneous Analysis of Microglia, Astrocytes and Infiltrating Lymphocytes by Flow Cytometry. , IBRO Rep Vol. 8 , 36-47 , 2020
Cells
Microglia, astrocytes, lymphocytes
Enzymes
Collagenase Type 2 : 120 u/ml
Papain : 20 u/ml
Neutral Protease : 1.2 u/ml
Medium
EBSS
Brain
Rat
Rat, SD, one month old
Bowman, P. , Betz, A. , Ar, D. , Wolinsky, J. , Penney, J. , Shivers, R. and Goldstein, G.
Primary Culture of Capillary Endothelium From Rat Brain ,
In Vitro
 17 (4) , 353 , 1981
Cells
Capillary endothelium
Pericytes
Enzymes
Medium
Medium 199
Colon
Human
Human
Rollo, B. , Zhang, D. , Stamp, L. , Menheniott, T. , Stathopoulos, L. , Denham, M. , Dottori, M. , King, S. , Hutson, J. and Newgreen, D.
Enteric Neural Cells from Hirschsprung Disease Patients Form Ganglia in Autologous Aneuronal Colon ,
Cell Mol Gastroenterol Hepatol
 2 , 92 , 2016
Cells
Colon enteric neural
Enzymes
Medium
Ham's F-12
Colon
Human
Human
Roig, A. , Eskiocak, U. , Hight, S. , Kim, S. , Delgado, O. , Souza, R. , Spechler, S. , Wright, W. and Shay, J.
Immortalized Epithelial Cells Derived from Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro. ,
Gastroenterol
 138 , 1012 , 2010
Cells
Colonic epithelial
Enzymes
Collagenase : 150 u/ml
Neutral Protease : 0.04 mg/ml
Medium
Basal X media
Colon
Human
Human
Fukushima, K. and Fiocchi, C.
Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. ,
Am J Physiol Gastrointest Liver Physiol
 286 , G804-13 , 2004
Cells
Colonic epithelial
Enzymes
Medium
RPMI 1640
Colon
Human
Human
Hisamatsu, T. , Watanabe, M. , Ogata, H. , Ezaki, T. , Hozawa, S. , Ishii, H. , Kanai, T. and Hibi, T.
Interferon-Inducible Gene Family 1-8U Expression in Colitis-Associated Colon Cancer and Severely Inflamed Mucosa in Ulcerative Colitis. ,
Cancer Res
 59 , 5927-31 , 1999
Cells
Epithelial and mucosal lymphocytes
Enzymes
CLSPA : 0.02%
Medium
RPMI 1640
Colon
Human
Human
Gibson, P. , Rosella, O. , Wilson, A. , Mariadason, J. , Rickard, K. , Byron, K. and Barkla, D.
Colonic Epithelial Cell Activation and the Paradoxical Effects of Butyrate. ,
Carcinogenesis
 20 , 539 , 1999
Cells
Colonic epithelial
Enzymes
Neutral Protease : 1.2 u/ml
Collagenase Type 4 : 50 u/ml
Medium
HBSS
Colon
Mouse
Mouse
Fattahi, F. , Steinbeck, J. , Kriks, S. , Tchieu, J. , Zimmer, B. , Kishinevsky, S. , Zeltner, N. , Mica, Y. , El-Nachef, W. , Zhao, H. , de Stanchina, E. , Gershon, M. , Grikscheit, T. , Chen, S. and Studer, L.
Deriving Human ENS Lineages for Cell Therapy and Drug Discovery in Hirschsprung Disease. ,
Nature
 531 , 105-9 , 2016
Cells
Colon organoid
Enzymes
Collagenase Type 1 : 800 u/ml
Neutral Protease : 0.013%
Medium
DMEM
Colon
Mouse
Mouse, 8-10 week
Round, J. and Mazmanian, S.
Inducible Foxp3+ Regulatory T-cell Development by a Commensal Bacterium of the Intestinal Microbiota. ,
Proc Natl Acad Sci U S A
 107 , 12204-9 , 2010
Cells
Lamina propria lymphocytes
Enzymes
Medium
HBSS
Colon
Mouse
Mouse
Atarashi, K. , Nishimura, J. , Shima, T. , Umesaki, Y. , Yamamoto, M. , Onoue, M. , Yagita, H. , Ishii, N. , Evans, R. , Honda, K. and Takeda, K.
ATP Drives Lamina Propria T(H)17 Cell Differentiation. ,
Nature
 455 , 808 , 2008
Cells
Lamina propria
Enzymes
Medium
RPMI 1640
Colon
Mouse
Mouse
Weigmann Benno, Tubbe Ingrid, Seidel Daniel, Nicolaev Alex, Becker Christoph, Neurath Markus F.
Isolation and subsequent analysis of murine lamina propria mononuclear cells from colonic tissue ,
Nat Protoc
 2 , 2307-11 , 2007
Cells
Lamina propria mononuclear cells
Enzymes
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.