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Tissue References Search Tool

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Endothelial
Human
Human, 18-68 yr
Li Wei, Sabater Alfonso L, Chen Ying-Ting, Hayashida Yasutaka, Chen Szu-Yu, He Hua, Tseng Scheffer C G.
A novel method of isolation, preservation, and expansion of human corneal endothelial cells ,
Inv Ophthal Visual Sci
 48 , 614-20 , 2007
Cells
Corneal endothelial
Enzymes
Medium
DMEM/F12
Endothelial
Human
Human
Muller AM, Hermanns MI, Skrzynski C, Nesslinger M, Muller KM. and Kirkpatrick CJ.
Expression of the endothelial markers PECAM-1, vWf, and CD34 in vivo and in vitro ,
Exp Mol Pathol
 72 , 221 , 2002
Cells
Pulmonary vascular endothelial cells
Enzymes
Neutral Protease : 1.18 u/ml
Elastase : 10 u/ml
Medium
M199
Endothelial
Human
Human
Farber HW, Antonov AS, Romanov YA, Smirnov VN, Scarfo LM, Beer DJ.
Cytokine secretion by human aortic endothelial cells is related to degree of atherosclerosis ,
Am J Physiol
 262 , H1088-95 , 1992
Cells
Vascular endothelial
Enzymes
Trypsin : 0.25%
Medium
M-199
Endothelial
Human
Human
Sherer, G. , Fitzharris, T. , Faulk, W. and LeRoy, E.
Cultivation of Microvascular Endothelial Cells from Human Preputial Skin ,
In Vitro
 16 , 675 , 1980
Cells
Microvascular endothelial
Neonatal foreskins
Enzymes
Neutral Protease : at 1000 u/ml
Medium
Konigsberg's modification of HBSS
(See Reference)
Endothelial
Mouse
Mouse
Luo, S. , Truong, A. and Makino, A.
Isolation of Mouse Coronary Endothelial Cells. ,
J Vis Exp
 , 2016
Cells
Coronary endothelial
Enzymes
Medium
Kreb's
Endothelial
Mouse
Mouse
Xiao, L. , McCann, J. and Dudley, A.
Isolation and Culture Expansion of Tumor-specific Endothelial Cells. ,
J Vis Exp
 , e53072 , 2015
Cells
Endothelial
Enzymes
Medium
DMEM
Endothelial
Mouse
Mouse
Kochukov, M. , Balasubramanian, A. , Abramowitz, J. , Birnbaumer, L. and Marrelli, S.
Activation of Endothelial Transient Receptor Potential C3 Channel is Required for Small Conductance Calcium-Activated Potassium Channel Activation and Sustained Endothelial Hyperpolarization and Vasodilation of Cerebral Artery. ,
J Am Heart Assoc
 3 , 2014
Cells
Cerebral artery endothelial
Enzymes
Neutral Protease : 4 u/ml
Elastase : 1 u/ml
Collagenase Type 1 : 120 u/ml
Medium
DMEM
Endothelial
Mouse
Mouse, neonatal
Cha, S. , Talavera, D. , Demir, E. , Nath, A. and Sierra-Honigmann, M.
A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. ,
Microvasc Res
 70 , 198 , 2005
Cells
Microvascular endothelial
Enzymes
Medium
DMEM
Endothelial
Porcine
Porcine, 6 month
Balaoing, L. , Post, A. , Lin, A. , Tseng, H. , Moake, J. and Grande-Allen, K.
Laminin Peptide-Immobilized Hydrogels Modulate Valve Endothelial Cell Hemostatic Regulation. ,
PLoS ONE
 10 , e0130749 , 2015
Cells
Valvular endothelial
Enzymes
Medium
PBS
Mammary
Mouse

Mouse, female

Sun, H. , Xu, X. and Deng, C.
Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands. ,
Bio Protoc
 10 , e3530 , 2020
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F-12
Mammary
Mouse
Mouse, 12 week
Taddei, I. , Deugnier, M. , Faraldo, M. , Petit, V. , Bouvard, D. , Medina, D. , Fassler, R. , Thiery, J. and Glukhova, M.
Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells ,
Nat Cell Biol
 10 , 716-22 , 2008
Cells
Mammary epithelial
Enzymes
Collagenase : 0.3%
Hyaluronidase : 100 u/ml
Trypsin : 0.25%
Medium
see reference
Mammary
Rat
Rat, SD
Brake P. , Zhang L. and Jefcoate C.
Aryl Hydrocarbon Receptor Regulation of Cytochrome P4501B1 in Rat Mammary Fibroblasts: Evidence for Transcriptional Repression by Glucocorticoids ,
Mol Pharmacol
 54 , 825 , 1998
Cells
Mammary fibroblasts
Enzymes
Medium
DMEM/F-12
Mammary
Rat
Rat, SD, 50 day
Varela L. , Darcy K. and Ip M.
The Epidermal Growth Factor Receptor is not Required for Tumor Necrosis Factor-alpha Action in Normal Mammary Epithelial Cells ,
Endocrinology
 138 , 3891 , 1997
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F12
Mammary
Rat
Rat, SD, female, 55 day
Ehmann, U. , Osborn, R. , Guzman, R. and Fajardo, L.
Cultured Proliferating Rat Mammary Epithelial Cells ,
In Vitro Cell Dev Biol
 27 , 749 , 1991
Cells
Epithelial
Enzymes
Neutral Protease : 3 u/ml
Medium
Medium 199
Mammary
Rat
Rat, 50-60 days
Hahm, H.A. and Ip, M.M.
Primary Culture of Normal Rat Mammary Epithelial Cells Within a Basement Matrix. 1. Regulation of Proliferation by Hormones and Growth Factors ,
In Vitro Cell Dev Biol
 26 , 791 , 1990
Cells
Epithelial
Enzymes
Medium
EBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.