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Tissue References Search Tool

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Epithelial
Bovine
Bovine, fetal
Schumann, B.L. , Cody, T.E. , Miller, M.L. and Leikauf, G.D.
Isolation, Characterization, and Long-Term Culture of Fetal Bovine Tracheal Epithelial Cells ,
In Vitro Cell Dev Biol
 24 , 211 , 1988
Cells
Epithelial
Tracheal
Enzymes
Medium
Dissociation medium, CMF
Epithelial
Human
Human
Gottipamula, S. , Saraswat, S. and Sridhar, K.
Comparative Study of Isolation, Expansion and Characterization of Epithelial Cells. ,
Cytotherapy
 19 , 263-271 , 2017
Cells
Epithelial
Enzymes
Medium
PBS
Epithelial
Human
Human
Fukushima, K. and Fiocchi, C.
Paradoxical Decrease of Mitochondrial DNA Deletions in Epithelial Cells of Active Ulcerative Colitis Patients. ,
Am J Physiol Gastrointest Liver Physiol
 286 , G804-13 , 2004
Cells
Colonic epithelial
Enzymes
Medium
RPMI 1640
Epithelial
Human
Human
Espana EM, Romano AC, Kawakita T, Di Pascuale M, Smiddy R. and Tseng SC
Novel enzymatic isolation of an entire viable human limbal epithelial sheet ,
Inv Ophthal Visual Sci
 44(10) , 4275 , 2003
Cells
Corneal limbal epithelial sheet
Enzymes
Medium
see reference
Epithelial
Human
Human
Smoot, D. , Sewchand, J. , Young, K. , Desbrodes, B. , Allen, C. and Naab, T.
A Method for Establishing Primary Cultures of Human Gastric Epithelial Cells ,
Meth Cell Sci
 22 , 133 , 2000
Cells
Gastric epithelial cells
Enzymes
Medium
L-15
Epithelial
Human
Human
Halbert CL, Alexander IE, Wolgamot GM, Miller AD.
Adeno-associated virus vectors transduce primary cells much less efficiently than immortalized cells ,
J Virol
 69 , 1473-9 , 1995
Cells
Nasal polyp epithelial
Enzymes
Trypsin : 0.1%
Medium
see reference
Epithelial
Mouse
Mouse
Sun, F. , Xiao, G. and Qu, Z.
Isolation of Murine Alveolar Type II Epithelial Cells. ,
Bio-protocol
 7 , 2017
Cells
Alveolar epithelial
Enzymes
Medium
DMEM
Epithelial
Mouse
Mouse
Mathew, R. , Degenhard,t K. , Haramaty, L. , Karp, C. and White, E.
Immortalized Mouse Epithelial Cell Models to Study the Role of Apoptosis in Cancer. ,
Methods Enzymol
 446 , 77-106 , 2008
Cells
Epithelial
Enzymes
Collagenase : 0.025%
Medium
DMEM
Epithelial
Porcine
Porcine
De Buysscher, E. , Kennedy, J. and Mendicino, J.
Synthesis of Mucin Glycoproteins by Epithelial Cells Isolated from Swine Trachea by Specific Proteolysis ,
In Vitro
 20 , 433 , 1984
Cells
Trachea
Enzymes
Medium
HBSS
Epithelial
Rabbit
Rabbit, New Zealand white, adult
Vidrich, A. , Racindranath, R. , Farsi, K. and Targan, S.
A Method for the Rapid Establishment of Normal Adult Mammalian Colonic Epithelial Cell Cultures ,
In Vitro Cell Dev Biol
 24 (3) , 188 , 1988
Cells
Colon
Enzymes
Medium
PBS
Epithelial
Rat
Rat, embryonic
Shannon JM, Gebb SA. and Nielsen LD
Induction of alveolar type II cell differentiation in embryonic tracheal epithelium in mesenchyme-free culture ,
Development
 126 , 1675 , 1999
Cells
Tracheal epithelial
Enzymes
Medium
DMEM/F12
Epithelial
Rat
Rat, 6 day old
Evans GS, Flint N, Somers AS, Eyden B. and Potten CS.
The development of a method for the preparation of rat intestinal epithelial cell primary cultures ,
J Cell Sci
 101 , 219 , 1992
Cells
Rat intestinal epithelial
Enzymes
Collagenase : 300 u/ml
Medium
DMEM
Epithelial
Rat
Rat, 6-8 day
Chang CW, Roque RS, Defoe DM. and Caldwell RB.
An improved method for isolation and culture of pigment epithelial cells from rat retina ,
Curr Eye Res
 10(11) , 1081 , 1991
Cells
Retinal pigment epithelial
Enzymes
Medium
DMEM
Intestine
Mouse
Mouse, P4
Hotta, R. , Stamp, L. , Foong, J. , McConnell, S. , Bergner, A. , Anderson, R. , Enomoto, H. , Newgreen, D. , Obermayr, F. , Furness, J. and Young, H.
Transplanted Progenitors Generate Functional Enteric Neurons in the Postnatal Colon. ,
J Clin Invest
 123 , 1182 , 2013
Cells
Enteric neural crest progenitors
Enzymes
Medium
DMEM/F12
Intestine
Mouse
Mouse
Xue, X. and Shah, Y.
In Vitro Organoid Culture of Primary Mouse Colon Tumors. ,
J Vis Exp
 , e50210 , 2013
Cells
Colon tumor organoids
Enzymes
Medium
DMEM
Intestine
Mouse
Mouse, 2-3 month
Becker, L. , Kulkarni, S. , Tiwari, G. , Micci, M. and Pasricha, P.
Divergent Fate and Origin of Neurosphere-Like Bodies from Different Layers of the Gut. ,
Am J Physiol Gastrointest Liver Physiol
 302 , G958-65 , 2012
Cells
Enteric neural stem cells
Enzymes
Medium
M199
Intestine
Mouse
Mouse
Barthel, E. , Speer, A. , Levin, D. , Sala, F. , Hou, X. , Torashima, Y. , Wigfall, C. and Grikscheit, T.
Tissue Engineering of the Intestine in a Murine Model. ,
J Vis Exp
 70 , e4279 , 2012
Cells
Intestinal organoids
Enzymes
Collagenase Type 1 : 800 u/ml
Neutral Protease : 0.013%
Medium
DMEM
Intestine
Mouse
Mouse
Ruiz, V. , Sachdev, M. , Zhang, S. , Wen, S. and Moss, S.
Isolating, Immunophenotyping and Ex vivo Stimulation of CD4+ and CD8+ Gastric Lymphocytes During Murine Helicobacter Pylori Infection. ,
J Immunol Methods
 384 , 157-63 , 2012
Cells
Lyphocytes
Enzymes
Medium
RPMI 1640

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.