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Tissue References Search Tool

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Lung
Human

Human

Konishi, S. , Tata, A. and Tata, P.
Defined Conditions for Long-term Expansion of Murine and Human Alveolar Epithelial Stem Cells in Three-dimensional Cultures. , STAR Protoc Vol. 3 , 101447 , 2022
Cells
Alveolar epithelial stem
Enzymes
Collagenase Type 1 : 450 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Human

Human

Youk, J. , Kim, T. , Evans, K. , Jeong, Y. , Hur, Y. , Hong, S. , Kim, J. , Yi, K. , Kim, S. , Na, K. , Bleazard, T. , Kim, H. , Fellows, M. , Mahbubani, K. , Saeb-Parsy, K. , Kim, S. , Kim, Y. , Koh, G. , Choi, B. , Ju, Y. and Lee, J.
Three-Dimensional Human Alveolar Stem Cell Culture Models Reveal Infection Response to SARS-CoV-2. , Cell Stem Cell Vol. 27 , 905-919.e10 , 2020
Cells
Aveolar
Enzymes
Medium
DMEM
Lung
Human
Human
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Type 2 Alveolar Cells are Stem Cells in Adult Lung ,
J Clin Invest
 123 , 3025 , 2013
Cells
Alveolar epithelial
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 0.25%
Elastase : 10 u/ml
Medium
Bronchial Epithelial Growth Medium
Lung
Human
Human
Fujino, N. , Kubo, H. , Suzuki, T. , Ota, C. , Hegab, A. , He, M. , Suzuki, S. , Suzuki, T. , Yamada, M. , Kondo, T. , Kato, H. and Yamaya, M.
Isolation of Alveolar Epithelial Type II Progenitor Cells from Adult Human Lungs. ,
Lab Invest
 91 , 363 , 2011
Cells
Lung
Enzymes
Medium
DMEM
Lung
Mouse

Mouse

Konishi, S. , Tata, A. and Tata, P.
Defined Conditions for Long-term Expansion of Murine and Human Alveolar Epithelial Stem Cells in Three-dimensional Cultures. , STAR Protoc Vol. 3 , 101447 , 2022
Cells
Alveolar epithelial stem
Enzymes
Collagenase Type 1 : 450 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Mouse
Mouse
Sun, F. , Xiao, G. and Qu, Z.
Isolation of Murine Alveolar Type II Epithelial Cells. ,
Bio-protocol
 7 , 2017
Cells
Alveolar epithelial
Enzymes
Medium
DMEM
Lung
Mouse
Mouse, 8-12 week
Singer, B. , Mock, J. , D'Alessio, F. , Aggarwal, N. , Mandke, P. , Johnston, L. and Damarla, M.
Flow-Cytometric Method for Simultaneous Analysis of Mouse Lung Epithelial, Endothelial, and Hematopoietic Lineage Cells. ,
Am J Physiol Lung Cell Mol Physiol
 310 , L796-801 , 2016
Cells
Epithelial, Endothelial
Enzymes
Medium
PBS
Lung
Mouse
Mouse
Mohapatra, A. , Van Dyken, S. , Schneider, C. , Nussbaum, J. , Liang, H. and Locksley, R.
Group 2 Innate Lymphoid Cells Utilize the IRF4-IL-9 Module to Coordinate Epithelial Cell Maintenance of Lung Homeostasis. ,
Mucosal Immunol
 , 2015
Cells
Alveolar
Enzymes
Collagenase Type 1 : 300 u/ml
Elastase : 4 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 200 u/ml
Medium
HBSS
Lung
Mouse
Mouse
Vaughan, A. , Halbert, C. , Wootton, S. and Miller, A.
Lung Cancer in Mice Induced by the Jaagsiekte Sheep Retrovirus Envelope Protein is not Maintained by Rare Cancer Stem Cells, but Tumorigenicity does Correlate with Wnt Pathway Activation. ,
Mol Cancer Res
 10 , 86 , 2012
Cells
Lung tumor
Enzymes
Neutral Protease : 50 u/ml
Collagenase : 400 u/ml
Medium
DMEM
Lung
Mouse
Mouse, 8-12 week
Driscoll, B. , Kikuchi, A. , Lau, A. , Lee, J. , Reddy, R. , Jesudason, E. , Kim, C. and Warburton, D.
Isolation and Characterization of Distal Lung Progenitor Cells. ,
Methods Mol Biol
 879 , 109 , 2012
Cells
Lung progenitor
Enzymes
Medium
DMEM
Lung
Mouse
Mouse
Rock, J, Barkauskas, C, Cronc, M, Xue, Y, Harris, J, Liang, J, Noble, P. and Hogan, B.
Multiple Stromal Populations Contribute to Pulmonary Fibrosis Without Evidence for Epithelial to Mesenchymal Transition. ,
Proc Natl Acad Sci U S A
 108 , E1475 , 2011
Cells
Lung
Enzymes
Collagenase Type 1 : 450 u/ml
Elastase : 4 u/ml
Neutral Protease : 5 u/ml
Deoxyribonuclease I : 0.33 u/ml
Medium
DMEM/F12
Lung
Mouse
Mouse
Paine R 3rd, Wilcoxen SE, Morris SB, Sartori C, Baleeiro CE, Matthay MA. and Christensen PJ
Transgenic overexpression of granulocyte macrophage-colony stimulating factor in the lung prevents hyperoxic lung injury ,
Am J Pathol
 163 , 2397 , 2003
Cells
Alveolar epithelial cells
Enzymes
Medium
DMEM
Stem
Human
Human
Randelli, P. , Menon, A. , Ragone, V. , Creo P. , Alfieri, M. , Perucca, O. , Banfi, G. , Cabitza, P. , Tettamanti, G. and Anastasia, L.
Effects of the Pulsed Electromagnetic Field PST on Human Tendon Stem Cells: a Controlled Laboratory Study. ,
BMC Complement Altern Med
 16 , 293 , 2016
Cells
Tendon stem
Enzymes
Medium
MEM
Stem
Human
Human
Wu, Z. , Wang, J. , Dong, R. , Wang, L. , Fan, Z. , Liu, D. and Wang, S.
Depletion of MEIS2 Inhibits Osteogenic Differentiation Potential of Human Dental Stem Cells. ,
Int J Clin Exp Med
 8 , 7220-30 , 2015
Cells
Dental mesenchymal stem
Enzymes
Medium
DMEM
Stem
Human
Human
Charville, G. , Cheung, T. , Yoo, B. , Santos, P. , Lee, G. , Shrager, J. and Rando, T.
Ex Vivo Expansion and In Vivo Self-Renewal of Human Muscle Stem Cells. ,
Stem Cell Reports
 5 , 621 , 2015
Cells
Skeletal muscle stem
Enzymes
Collagenase Type 2 : 750 u/ml
Neutral Protease : 2 u/ml
Medium
Ham's F10
Stem
Human
Human
Barkauskas, C. , Cronce, M. , Rackley, C. , Bowie, E. , Keene, D. , Stripp, B. , Randell, S. , Noble, P. and Hogan, B.
Type 2 Alveolar Cells are Stem Cells in Adult Lung ,
J Clin Invest
 123 , 3025 , 2013
Cells
Alveolar epithelial
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 0.25%
Elastase : 10 u/ml
Medium
Bronchial Epithelial Growth Medium
Stem
Human
Human, adult
Nagel, S. , Rohr, F. , Weber, C. , Kier, J. , Siemers, F. , Kruse, C. , Danner, S. , Brandenburger, M. and Matthiessen, A.
Multipotent Nestin-Positive Stem Cells Reside in the Stroma of Human Eccrine and Apocrine Sweat Glands and can be Propagated Robustly In Vitro. ,
PLoS ONE
 8 , e78365 , 2013
Cells
Epidermal stem cells
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 2.5%
Medium
DMEM
Stem
Human
Human
Salmon, B. , Bardet, C, Khaddam, M. Naji, J. , Coyac, B. , Baroukh, B. , Letourneur, F. , Lesieur, J. , Decup, F. , Le Denmat, D. , Nicoletti, A. , Poliard, A, Rowe, P. , Huet, E. , Vital, S. and Linglart, A.
MEPE-Derived ASARM Peptide Inhibits Odontogenic Differentiation of Dental Pulp Stem Cells and Impairs Mineralization in Tooth Models of X-linked Hypophosphatemia. ,
PLoS ONE
 8 , e56749 , 2013
Cells
Dental pulp stem cells
Enzymes
Medium
DMEM
Stem
Human
Human
Patel, J. , Seppanen, E. , Chong, M. , Yeo, J. , Teo, E. , Chan, J. , Fisk, N. and Khosrotehrani, K.
Prospective Surface Marker-Based Isolation and Expansion of Fetal Endothelial Colony-Forming Cells from Human Term Placenta. ,
Stem Cells Transl Med
 2 , 839-47 , 2013
Cells
Endothelial colony forming
Enzymes
Medium
HBSS
Stem
Human
Human
Bonnamain, V. , Thinard, R. , Sergent-Tanguy, S. , Huet, P. , Bienvenu, G. , Naveilhan, P. , Farges, J. and Alliot-Licht, B.
Human Dental Pulp Stem Cells Cultured in Serum-Free Supplemented Medium. ,
Front Physiol
 4 , 357 , 2013
Cells
Dental pulp stem
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Sakai, K. , Yamamoto, A. , Matsubara, K. , Nakamura, S. , Naruse, M. , Yamagata, M. , Sakamoto, K. , Tauchi, R. , Wakao, N. , Imagama, S. , Hibi, H. , Kadomatsu, K. , Ishiguro, N. and Ueda, M
Human Dental Pulp-Derived Stem Cells Promote Locomotor Recovery After Complete Transection of the Rat Spinal Cord by Multiple Neuro-Regenerative Mechanisms. ,
J Clin Invest
 122 , 80 , 2012
Cells
Dental pulp derived stem
Enzymes
Medium
DMEM
Stem
Human
Human
Nazarov, I. , Lee, J. , Soupene, E. , Etemad, S. , Knapik, D. , Green, W. , Bashkirova, E. , Fang, X. , Matthay, M. , Kuypers, F. and Serikov, V.
Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential. ,
Stem Cells Transl Med
 1 , 359-72 , 2012
Cells
Chorionic mesenchymal
Enzymes
Neutral Protease : 2.5 u/ml
Trypsin : 0.25%
Medium
MEM
Stem
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Stem
Human
Human
Farias, V. , Linares-Fernandez, J. , Penalver, J. , Paya Colmenero, J. , Ferron, G. , Duran, E. , Fernandez, R. , Olivares, E. , O'Valle, F. , Puertas, A. , Oliver, F. and Ruiz de Almodovar, J.
Human Umbilical Cord Stromal Stem Cell Express CD10 and Exert Contractile Properties. ,
Placenta
 32 , 86 , 2011
Cells
Umbilical cord stromal stem
Enzymes
Medium
DMEM
Stem
Human
Human
Roig, A. , Eskiocak, U. , Hight, S. , Kim, S. , Delgado, O. , Souza, R. , Spechler, S. , Wright, W. and Shay, J.
Immortalized Epithelial Cells Derived from Human Colon Biopsies Express Stem Cell Markers and Differentiate In Vitro. ,
Gastroenterol
 138 , 1012 , 2010
Cells
Colonic epithelial
Enzymes
Collagenase : 150 u/ml
Neutral Protease : 0.04 mg/ml
Medium
Basal X media
Stem
Human
Human
Du, Y. , Roh, D. , Funderburgh, M. , Mann, M. , Marra, K. , Rubin, J. , Li, X. and Funderburgh, J.
Adipose-Derived Stem Cells Differentiate to Keratocytes In Vitro. ,
Mol Vis
 16 , 2680 , 2010
Cells
Corneal stromal stem
Enzymes
Neutral Protease : 1.2 u/ml
Collagenase : 0.1%
Medium
DMEM
Stem
Human
Human
Huang, G. , Yamaza, T. , Shea, L. , Djouad, F. , Kuhn, N. , Tuan, R. and Shi, S.
Stem/Progenitor Cell-Mediated De Novo Regeneration of Dental Pulp with Newly Deposited Continuous Layer of Dentin in an In Vivo Model. ,
Tissue Eng Part A
 16 , 605 , 2010
Cells
Dental pulp and apical papilla stem cells
Enzymes
Medium
MEM
Stem
Human
Human
Zhang, Q. , Yamaza, T. , Kelly, AP, Shi, S. , Wang, S. , Brown, J. , Wang, L. , French, S. , Shi, S. and Le, A.
Tumor-like Stem Cells Derived from Human Keloid are Governed by the Inflammatory Niche Driven by IL-17/IL-6 Axis. ,
PLoS ONE
 4 , e7798 , 2009
Cells
Skin and keloid progenitor
Enzymes
Medium
PBS
Stem
Human
Human
Nakamura, T. , Endo, K. and Kinoshita, S.
Identification of Human Oral Keratinocyte Stem/Progenitor Cells by Neurotrophin Receptor p75 and the Role of Neurotrophin/p75 Signaling. ,
Stem Cells
 25 , 628-38 , 2007
Cells
Oral epithelial
Enzymes
Neutral Protease : 1.2 u/ml
Trypsin : 0.05%
Medium
PBS
Stem
Human
Human, 8-12 year
Bi Yanming, Ehirchiou Driss, Kilts Tina M, Inkson Colette A, Embree Mildred C, Sonoyama Wataru, Li Li, Leet Arabella I, Seo Byoung-Moo, Zhang Li, Shi Songtao, Young Marian F.
Identification of tendon stem/progenitor cells and the role of the extracellular matrix in their niche ,
Nat Med
 13 , 1219-27 , 2007
Cells
Tendon stem/progenitor
Enzymes
Medium
DMEM
Stem
Human
Human
Steigman, S. and Fauza, D.
Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta. ,
Curr Protoc Stem Cell Biol
 Chapter 1 , Unit 1E.2 , 2007
Cells
Mesenchymal stem
Enzymes
Neutral Protease : see reference
Medium
CMF-DPBS
Stem
Human
Human
Yu Hong, Fang Dong, Kumar Suresh M, Li Ling, Nguyen Thiennga K, Acs Geza, Herlyn Meenhard, Xu Xiaowei.
Isolation of a novel population of multipotent adult stem cells from human hair follicles ,
Am J Pathol
 168 , 1879-88 , 2006
Cells
Hair follicular epithelial
Enzymes
Trypsin : 0.25%
Medium
DMEM/F-12
Stem
Human
Human
Portmann-Lanz CBettina, Schoeberlein Andreina, Huber Alexander, Sager Ruth, Malek Antoine, Holzgreve Wolfgang, Surbek DanielV.
Placental mesenchymal stem cells as potential autologous graft for pre- and perinatal neuroregeneration ,
Am J Obstet Gynecol
 194 , 664-73 , 2006
Cells
Placental mesenchymal stem
Enzymes
Collagenase Type 2 : 270 u/ml
Neutral Protease : 2.4 u/ml
Medium
MEM
Stem
Human
Human, neonatal
Li, A. , Pouliot, N. , Redvers, R. and Kaur, P.
Extensive tissue-regenerative capacity of neonatal human keratinocytes stem cells and their progeny ,
J Clin Invest
 113 , 390-400 , 2004
Cells
Keratinocytes
Enzymes
Medium
DMEM
Stem
Human
Human, adult, male
Papini S, Cecchetti D, Campani D, Fitzgerald W, Grivel J, Chen S, Margolis L, Revoltella R.
Isolation and Clonal Analysis of Human Epidermal Keratinocyte Stem Cells in Long-term Culture ,
Stem Cells
 21 , 481 , 2003
Cells
Human epidermal keratinocyte stem cells
Enzymes
Medium
DMEM
Stem
Human
Human, 7-8 yr
Miura, M. , Gronthos, S, Zhao, M, Lu, B, Fisher, L, Robey, P. and Shi, S.
SHED: Stem Cells from Human Exfoliated Deciduous Teeth ,
Proc Natl Acad Sci U S A
 100 , 5807 , 2003
Cells
Stem cells Human exfoliated deciduous teeth
Enzymes
Medium
see reference

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.