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Tissue References Search Tool

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Mammary
Mouse

Mouse, female

Sun, H. , Xu, X. and Deng, C.
Preparation of Single Epithelial Cells Suspension from Mouse Mammary Glands. ,
Bio Protoc
 10 , e3530 , 2020
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F-12
Mammary
Mouse
Mouse, 12 week
Taddei, I. , Deugnier, M. , Faraldo, M. , Petit, V. , Bouvard, D. , Medina, D. , Fassler, R. , Thiery, J. and Glukhova, M.
Beta1 Integrin Deletion from the Basal Compartment of the Mammary Epithelium Affects Stem Cells ,
Nat Cell Biol
 10 , 716-22 , 2008
Cells
Mammary epithelial
Enzymes
Collagenase : 0.3%
Hyaluronidase : 100 u/ml
Trypsin : 0.25%
Medium
see reference
Mammary
Rat
Rat, SD
Brake P. , Zhang L. and Jefcoate C.
Aryl Hydrocarbon Receptor Regulation of Cytochrome P4501B1 in Rat Mammary Fibroblasts: Evidence for Transcriptional Repression by Glucocorticoids ,
Mol Pharmacol
 54 , 825 , 1998
Cells
Mammary fibroblasts
Enzymes
Medium
DMEM/F-12
Mammary
Rat
Rat, SD, 50 day
Varela L. , Darcy K. and Ip M.
The Epidermal Growth Factor Receptor is not Required for Tumor Necrosis Factor-alpha Action in Normal Mammary Epithelial Cells ,
Endocrinology
 138 , 3891 , 1997
Cells
Mammary epithelial
Enzymes
Medium
DMEM/F12
Mammary
Rat
Rat, SD, female, 55 day
Ehmann, U. , Osborn, R. , Guzman, R. and Fajardo, L.
Cultured Proliferating Rat Mammary Epithelial Cells ,
In Vitro Cell Dev Biol
 27 , 749 , 1991
Cells
Epithelial
Enzymes
Neutral Protease : 3 u/ml
Medium
Medium 199
Mammary
Rat
Rat, 50-60 days
Hahm, H.A. and Ip, M.M.
Primary Culture of Normal Rat Mammary Epithelial Cells Within a Basement Matrix. 1. Regulation of Proliferation by Hormones and Growth Factors ,
In Vitro Cell Dev Biol
 26 , 791 , 1990
Cells
Epithelial
Enzymes
Medium
EBSS
Tumor
Hamster
Hamster, 6 week old
Min, B. , Kim, K. , Cherrick, H. and Park, N.
Three Cell Lines from Hamster Buccal Pouch Tumors Induced by Topical 7,12-Dimethylbenz(a)Anthracene, Alone or in Conjunction with Herpes Simplex Virus Inoculation ,
In Vitro Cell Dev Biol
 27A , 128 , 1991
Cells
Buccal pouch
Enzymes
Medium
CMF HBSS
Tumor
Human
Human
Ayuso, J. , Vitek, R. , Swick, A. , Skala, M. , Wisinski, K. , Kimple, R. , Lambert, P. and Beebe, D.
Effects of Culture Method on Response to EGFR Therapy in Head and Neck Squamous Cell Carcinoma Cells ,
Sci Rep
 9 , 12480 , 2019
Cells
Fibroblasts
Enzymes
Medium
FM Fibroblast media
Tumor
Human
Human
Jiang, C. , Zhang, Q. , Shanti, R. , Shi, S. , Chang, T. , Carrasco, L. , Alawi, F. and Le, A.
Mesenchymal Stromal Cell-Derived Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Acquisition of Epithelial Stem-Like Cell Properties in Ameloblastoma Epithelial Cells. ,
Stem Cells
 35 , 2083-2094 , 2017
Cells
Ameloblastoma epithelial
Enzymes
Collagenase : 0.1%
Neutral Protease : 50 u/ml
Medium
MEM
Tumor
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Tumor
Human
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Tumor
Human
Human
Boyd, J. , Rinehart Jr. , Walton, L. , Siegal, G. and Kaufman, D.
Ultrastructural Characterization of Two New Human Endometrial Carcinoma Cell Lines and Normal Human Endometrial Epithelial Cells Cultured on Extracellular Matrix ,
In Vitro Cell Dev Biol
 26 , 701 , 1990
Cells
Tumor
Enzymes
Medium
DMEM/Ham's F-12
Tumor
Human
Human, 9-74 year
Sheela S, Riccardi VM, Ratner N.
Angiogenic and invasive properties of neurofibroma Schwann cells ,
J Cell Biol
 111 , 645-53 , 1990
Cells
Neurofibroma
Enzymes
Medium
L-15
Tumor
Mouse
Mouse
Aguilera, T. , Rafat, M. , Castellini, L. , Shehade, H. , Kariolis, M. , Hui, A. , Stehr, H. , von Eyben, R. , Jiang, D. , Ellies, L. , Koong, A. , Diehn, M. , Rankin, E. Graves, E. and Giaccia, A.
Reprogramming the Immunological Microenvironment through Radiation and Targeting Axl. ,
Nat Commun
 7 , 13898 , 2016
Cells
Tumor
Enzymes
Collagenase : 0.1%
Neutral Protease : 0.5 u/ml
Medium
F12K
Tumor
Mouse
Mouse
Filtjens, J. , Keirsse, J. , Van Ammel, E. , Taveirne, S. , Van Acker, A. , Kerre, T. , Taghon, T. , Vandekerckhove, B. , Plum, J. , Van Ginderachter, J. and Leclercq, G.
Expression of the Inhibitory Ly49E Receptor is Not Critically Involved in the Immune Response Against Cutaneous, Pulmonary or Liver tumours. ,
Sci Rep
 6 , 30564 , 2016
Cells
Tumor
Enzymes
Medium
RPMI-1640
Tumor
Mouse
Mouse
Xiao, L. , McCann, J. and Dudley, A.
Isolation and Culture Expansion of Tumor-specific Endothelial Cells. ,
J Vis Exp
 , e53072 , 2015
Cells
Endothelial
Enzymes
Medium
DMEM
Tumor
Mouse
Mouse
Dunleavey, J. , Xiao, L. , Thompson, J. , Kim, M. , Shields, J. , Shelton, S. , Irvin, D. , Brings, V. , Ollila, D. , Brekken, R. , Dayton, P. , Melero-Martin, J. and Dudley, A.
Vascular Channels Formed by Subpopulations of PECAM1+ Melanoma Cells. ,
Nat Commun
 5 , 5200 , 2014
Cells
Tumor
Enzymes
Medium
DMEM
Tumor
Mouse
Mouse
Vaughan, A. , Halbert, C. , Wootton, S. and Miller, A.
Lung Cancer in Mice Induced by the Jaagsiekte Sheep Retrovirus Envelope Protein is not Maintained by Rare Cancer Stem Cells, but Tumorigenicity does Correlate with Wnt Pathway Activation. ,
Mol Cancer Res
 10 , 86 , 2012
Cells
Lung tumor
Enzymes
Neutral Protease : 50 u/ml
Collagenase : 400 u/ml
Medium
DMEM
Tumor
Mouse
Mouse
Rasheed, Z. , Wang, Q. and Matsui, W.
Isolation of Stem Cells from Human Pancreatic Cancer Xenografts. ,
J Vis Exp
 43 , 2169 , 2010
Cells
Pancreatic cancer stem
Enzymes
Collagenase Type 4 : 200 u/ml
Neutral Protease : 0.6 u/ml
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.