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Tissue References Search Tool

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Skin
Canine
Canine
Zheng, Y. , Nace, A. , Chen, W. , Watkins, K. , Sergott, L. , Homan, Y. , Vandeberg, J. , Breen, M. and Stenn, K.
Mature Hair Follicles Generated from Dissociated Cells: A Universal Mechanism of Folliculoneogenesis. ,
Dev Dyn
 239 , 2619-26 , 2010
Cells
Epidermal
Enzymes
Collagenase : 0.35%
Medium
DMEM
Skin
Goat
Goat
Deng, Y. , Hu, J. and Athanasiou, K.
Isolation and Chondroinduction of a Dermis-Isolated, Aggrecan-Sensitive Subpopulation with High Chondrogenic Potential. ,
Arthritis Rheum
 56 , 168 , 2007
Cells
Dermis isolated aggrecan sensitive
Enzymes
Medium
DMEM
Skin
Human

Human

Burja, B. , Paul, D. , Tastanova, A. , Edalat, S. , Gerber, R. , Houtman, M. , Elhai, M. , Bürki, K. , Staeger, R. , Restivo, G. , Lang, R. , Sodin-Semrl, S. , Lakota, K. , Tomšič, M. , Levesque, M. , Distler, O. , Rotar, �. , Robinson, M. and Frank-Bertoncelj, M.
An Optimized Tissue Dissociation Protocol for Single-Cell RNA Sequencing Analysis of Fresh and Cultured Human Skin Biopsies. , Front Cell Dev Biol Vol. 10 , 872688 , 2022
Cells
Skin
Enzymes
Neutral Protease : 2.4 u/ml
Collagenase Type 4 : 1,000 u/ml
Medium
RPMI
Skin
Human
Human
Botting, Ra. , Bertram, K. , Baharlou, H. , Sandgren, K. , Fletcher, J. , Rhodes, J. , Rana, H. , Plasto, T. , Wang, X. , Lim, J. , Barnouti, L. , Kohout, M. , Papadopoulos, T. , Merten, S. , Olbourne, N. and Cunningham, A.
Phenotypic and Functional Consequences of Different Isolation Protocols on Skin Mononuclear Phagocytes. ,
J Leukoc Biol
 101 , 1393-1403 , 2017
Cells
Mononuclear phagocytes
Enzymes
Neutral Protease : 1 u/ml
Collagenase : 200 u/ml
Trypsin : 0.5%
Medium
RPMI-1640
Skin
Human
Human
Chen, Z. , Wang, X. , Jin, T. , Wang, Y. , Hong, C. , Tan, L. , Dai, T. , Wu, L. , Zhuang, Z. and Shi, C.
Increase in the Radioresistance of Normal Skin Fibroblasts but not Tumor Cells by Mechanical Injury. ,
Cell Death Dis
 8 , e2573 , 2017
Cells
Fibroblasts
Enzymes
Medium
DMEM
Skin
Human
Human
Cheuk, S. , Wiken, M. , Blomqvist, L. , Nylen, S. , Talme, T. and Stahle, M.and Eidsmo, L.
Epidermal Th22 and Tc17 Cells Form a Localized Disease Memory in Clinically Healed Psoriasis. ,
J Immunol
 192 , 3111 , 2014
Cells
Skin
Enzymes
Medium
RPMI 1640
Skin
Human
Human
Cerny, D. , Haniffa, M. , Shin, A. , Bigliardi, P. , Tan, B. , Lee, B. , Poidinger, M. , Tan, E. , Ginhoux, F. and Fink, K.
Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. ,
PLoS Pathog
 10 , e1004548 , 2014
Cells
Skin
Enzymes
Medium
RPMI
Skin
Human
Human, adult
Nagel, S. , Rohr, F. , Weber, C. , Kier, J. , Siemers, F. , Kruse, C. , Danner, S. , Brandenburger, M. and Matthiessen, A.
Multipotent Nestin-Positive Stem Cells Reside in the Stroma of Human Eccrine and Apocrine Sweat Glands and can be Propagated Robustly In Vitro. ,
PLoS ONE
 8 , e78365 , 2013
Cells
Epidermal stem cells
Enzymes
Neutral Protease : 2 u/ml
Trypsin : 2.5%
Medium
DMEM
Skin
Human
Human
Carriel, V. , Garzon, I. , Jimenez, J. , Oliveira, A. , Arias-Santiago, S. , Campos, A. , Sanchez-Quevedo, M. and Alaminos, M.
Epithelial and Stromal Developmental Patterns in a Novel Substitute of the Human Skin Generated with Fibrin-Agarose Biomaterials. ,
Cells Tissues Organs
 196 , 1-12 , 2012
Cells
Fibroblasts and keratinocytes
Enzymes
Medium
DMEM
Skin
Human
Human
Zheng, Y. , Nace, A. , Chen, W. , Watkins, K. , Sergott, L. , Homan, Y. , Vandeberg, J. , Breen, M. and Stenn, K.
Mature Hair Follicles Generated from Dissociated Cells: A Universal Mechanism of Folliculoneogenesis. ,
Dev Dyn
 239 , 2619-26 , 2010
Cells
Epidermal
Enzymes
Collagenase : 0.35%
Medium
DMEM
Skin
Human
Human
Zhang, Q. , Yamaza, T. , Kelly, AP, Shi, S. , Wang, S. , Brown, J. , Wang, L. , French, S. , Shi, S. and Le, A.
Tumor-like Stem Cells Derived from Human Keloid are Governed by the Inflammatory Niche Driven by IL-17/IL-6 Axis. ,
PLoS ONE
 4 , e7798 , 2009
Cells
Skin and keloid progenitor
Enzymes
Medium
PBS
Skin
Human
Human, 6-12 year
Chen, F. , Zhang, W. , Bi, D. , Liu, W. , Wei, X. , Chen, F. , Zhu, L. , Cui, L. and Cao, Y.
Clonal Analysis of Nestin(-) Vimentin(+) Multipotent Fibroblasts Isolated from Human Dermis. ,
J Cell Sci
 120 , 2875 , 2007
Cells
Dermal fibroblasts
Enzymes
Medium
DMEM
Skin
Human
Human
Yu Hong, Fang Dong, Kumar Suresh M, Li Ling, Nguyen Thiennga K, Acs Geza, Herlyn Meenhard, Xu Xiaowei.
Isolation of a novel population of multipotent adult stem cells from human hair follicles ,
Am J Pathol
 168 , 1879-88 , 2006
Cells
Hair follicular epithelial
Enzymes
Trypsin : 0.25%
Medium
DMEM/F-12
Skin
Human
Human, neonatal
Li, A. , Pouliot, N. , Redvers, R. and Kaur, P.
Extensive tissue-regenerative capacity of neonatal human keratinocytes stem cells and their progeny ,
J Clin Invest
 113 , 390-400 , 2004
Cells
Keratinocytes
Enzymes
Medium
DMEM
Skin
Human
Human
Babina Magda, Guhl Sven, Starke Andrae, Kirchhof Loreen, Zuberbier Torsten, Henz Beate M.
Comparative cytokine profile of human skin mast cells from two compartments--strong resemblance with monocytes at baseline but induction of IL-5 by IL-4 priming ,
J Leukoc Biol
 75 , 244-52 , 2004
Cells
Mast cells
Enzymes
Medium
RPMI
Skin
Human
Human
Supp Dorothy M, Wilson-Landy Kaila, Boyce Steven T.
Human dermal microvascular endothelial cells form vascular analogs in cultured skin substitutes after grafting to athymic mice ,
FASEB J
 16 , 797-804 , 2002
Cells
Keratinocytes, fibroblasts, endothelial
Enzymes
Neutral Protease : see reference
Collagenase : see reference
Medium
see reference
Skin
Human
Human, adult
Baudoux, B. , Castanares-Zapatero, D. , Leclercq-Smekens, M. , Berna, N. and Poumay, Y.
The Tetraspanin CD9 Associates with the Integrin a6B4 in Cultured Human Epidermal Keratinocytes and is Involved in Cell Motility ,
Eur J Cell Biol
 79 , 41 , 2000
Cells
Keratinocytes
Enzymes
Medium
PBS
Skin
Human
Human
Hybbinette S, Bostrom M, Lindberg K.
Enzymatic dissociation of keratinocytes from human skin biopsies for in vitro cell propagation ,
Exp Dermatol
 8 , 30-8 , 1999
Cells
Keratinocytes
Enzymes
Trypsin : 0.17%
Thermolysin : 0.05%
Medium
see reference
Skin
Human
Human
Grutzkau A, Kruger-Krasagakes S, Baumeister H, Schwarz C, Kogel H, Welker P, Lippert U, Henz BM, Moller A.
Synthesis, storage, and release of vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) by human mast cells: implications for the biological significance of VEGF206 ,
Mol Biol Cell
 9 , 875-84 , 1998
Cells
Human skin mast cells
Enzymes
Collagenase Type 1 : see reference
Medium
PBS
Skin
Human
Human
Reece J C, Handley A J, Anstee E J, Morrison W A, Crowe S M, Cameron P U.
HIV-1 selection by epidermal dendritic cells during transmission across human skin ,
J Exp Med
 187 , 1623-31 , 1998
Cells
Epidermal
Enzymes
Medium
PBS
Skin
Mouse
Mouse
Spidale, N. , Malhotra, N. , Frascoli, M. , Sylvia, K. , Miu, B. , Freeman, C. , Stadinski, B. , Huseby, E. and Kang, J.
Neonatal-Derived IL-17 Producing Dermal gd T Cells are Required to Prevent Spontaneous Atopic Dermatitis ,
Elife
 9 , 5118 , 2020
Cells
Keratinocytes
Enzymes
Medium
DMEM
Skin
Mouse
Mouse
Cerny, D. , Haniffa, M. , Shin, A. , Bigliardi, P. , Tan, B. , Lee, B. , Poidinger, M. , Tan, E. , Ginhoux, F. and Fink, K.
Selective Susceptibility of Human Skin Antigen Presenting Cells to Productive Dengue Virus Infection. ,
PLoS Pathog
 10 , e1004548 , 2014
Cells
Skin
Enzymes
Medium
RPMI
Skin
Mouse
Mouse
Gu, D. , Fan, Q. and Xie, J.
Cell Population Analyses During Skin Carcinogenesis. ,
J Vis Exp
 , e50311 , 2013
Cells
Epidermal
Enzymes
Medium
DMEM
Skin
Mouse
Mouse, neonatal
Cha, S. , Talavera, D. , Demir, E. , Nath, A. and Sierra-Honigmann, M.
A Method of Isolation and Culture of Microvascular Endothelial Cells from Mouse Skin. ,
Microvasc Res
 70 , 198 , 2005
Cells
Microvascular endothelial
Enzymes
Medium
DMEM
Skin
Mouse
Mouse, male 6-24 week
Montanaro F, Liadaki K, Volinski J, Flint A. and Kunkel LM.
Skeletal muscle engraftment potential of adult mouse skin side population cells ,
Proc Natl Acad Sci U S A
 100 , 9336 , 2003
Cells
Skin side population
Enzymes
Medium
PBS
Skin
Porcine
Porcine, 2-6 month
Regauer S, Compton C.
Cultured porcine epithelial grafts: an improved method ,
J Invest Dermatol
 94 , 230-4 , 1990
Cells
Keratinocytes
Enzymes
Medium
Dulbecco-Vogt MEM
Skin
Rabbit

Rabbit, Leporidae

Santos, F. , Carvalho, C. , Almeida, I. , Fagulha, T. , Rammos, F. , Barros, S. , Henriques, M. , Luís, T. and Duarte, M.
Simple Method for Establishing Primary Leporidae Skin Fibroblast Cultures. ,
Cells
 10 , 2021
Cells
Fibroblast
Enzymes
Trypsin : 0.25%
Medium
DMEM/HAMSF12
Tumor
Hamster
Hamster, 6 week old
Min, B. , Kim, K. , Cherrick, H. and Park, N.
Three Cell Lines from Hamster Buccal Pouch Tumors Induced by Topical 7,12-Dimethylbenz(a)Anthracene, Alone or in Conjunction with Herpes Simplex Virus Inoculation ,
In Vitro Cell Dev Biol
 27A , 128 , 1991
Cells
Buccal pouch
Enzymes
Medium
CMF HBSS
Tumor
Human
Human
Ayuso, J. , Vitek, R. , Swick, A. , Skala, M. , Wisinski, K. , Kimple, R. , Lambert, P. and Beebe, D.
Effects of Culture Method on Response to EGFR Therapy in Head and Neck Squamous Cell Carcinoma Cells ,
Sci Rep
 9 , 12480 , 2019
Cells
Fibroblasts
Enzymes
Medium
FM Fibroblast media
Tumor
Human
Human
Jiang, C. , Zhang, Q. , Shanti, R. , Shi, S. , Chang, T. , Carrasco, L. , Alawi, F. and Le, A.
Mesenchymal Stromal Cell-Derived Interleukin-6 Promotes Epithelial-Mesenchymal Transition and Acquisition of Epithelial Stem-Like Cell Properties in Ameloblastoma Epithelial Cells. ,
Stem Cells
 35 , 2083-2094 , 2017
Cells
Ameloblastoma epithelial
Enzymes
Collagenase : 0.1%
Neutral Protease : 50 u/ml
Medium
MEM
Tumor
Human
Human, 25-81 yr
Volovitz, I, Shapira, N. , Ezer, H. , Gafni, A. , Lustgarten, M. , Alter, T. , Ben-Horin, I. , Barzilai, O. , Shahar, T. , Kanner, A. , Fried, I. , Veshchev, I. , Grossman, R. and Ram, Z,
A Non-Aggressive, Highly Efficient, Enzymatic Method for Dissociation of Human Brain-Tumors and Brain-Tissues to Viable Single-Cells. ,
BMC Neurosci
 17 , 30 , 2016
Cells
Brain tumor
Enzymes
Neutral Protease : 0.11 u/ml
Hyaluronidase : 1,000 u/ml
Medium
HBSS
Tumor
Human
Human
Hussein, D. , Punjaruk, W. , Storer, L. , Shaw, L. , Othman, R. , Ottoman, R. , Peet, A. , Miller, S. , Bandopadhyay, G. , Heath, R. , Kumari, R. , Bowman, K. , Braker, P. , Rahman, R. , Jones, G. , Watson, S. and Lowe, J.
Pediatric Brain Tumor Cancer Stem Cells: Cell Cycle Dynamics, DNA Repair, and Etoposide Extrusion. ,
Neuro Oncol
 13 , 70-83 , 2011
Cells
Brain tumor
Enzymes
Medium
DMEM/F12
Tumor
Human
Human
Boyd, J. , Rinehart Jr. , Walton, L. , Siegal, G. and Kaufman, D.
Ultrastructural Characterization of Two New Human Endometrial Carcinoma Cell Lines and Normal Human Endometrial Epithelial Cells Cultured on Extracellular Matrix ,
In Vitro Cell Dev Biol
 26 , 701 , 1990
Cells
Tumor
Enzymes
Medium
DMEM/Ham's F-12
Tumor
Human
Human, 9-74 year
Sheela S, Riccardi VM, Ratner N.
Angiogenic and invasive properties of neurofibroma Schwann cells ,
J Cell Biol
 111 , 645-53 , 1990
Cells
Neurofibroma
Enzymes
Medium
L-15
Tumor
Mouse
Mouse
Aguilera, T. , Rafat, M. , Castellini, L. , Shehade, H. , Kariolis, M. , Hui, A. , Stehr, H. , von Eyben, R. , Jiang, D. , Ellies, L. , Koong, A. , Diehn, M. , Rankin, E. Graves, E. and Giaccia, A.
Reprogramming the Immunological Microenvironment through Radiation and Targeting Axl. ,
Nat Commun
 7 , 13898 , 2016
Cells
Tumor
Enzymes
Collagenase : 0.1%
Neutral Protease : 0.5 u/ml
Medium
F12K
Tumor
Mouse
Mouse
Filtjens, J. , Keirsse, J. , Van Ammel, E. , Taveirne, S. , Van Acker, A. , Kerre, T. , Taghon, T. , Vandekerckhove, B. , Plum, J. , Van Ginderachter, J. and Leclercq, G.
Expression of the Inhibitory Ly49E Receptor is Not Critically Involved in the Immune Response Against Cutaneous, Pulmonary or Liver tumours. ,
Sci Rep
 6 , 30564 , 2016
Cells
Tumor
Enzymes
Medium
RPMI-1640

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.