For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Brain
Ovine
Ovine
Reddy, R. , Amodei, R. , Estill, C. , Stormshak, F. , Meaker, M. and Roselli, C.
Effect of Testosterone on Neuronal Morphology and Neuritic Growth of Fetal Lamb Hypothalamus-Preoptic Area and Cerebral Cortex in Primary Culture. ,
PLoS ONE
 10 , e0129521 , 2015
Cells
Neurons
Enzymes
Papain : 0.2%
Medium
Hibernate
Brain
Ovine
Ovine
Lepore G, Gadau S, Mura A, Zedda M, Farina V.
Aromatase Immunoreactivity in Fetal Ovine Neuronal Cell Cultures Exposed to Oxidative Injury. ,
Eur J Histochem
 53 , e28 , 2009
Cells
Neurons
Enzymes
PDS kit : per instructions
Medium
DMEM
Brain
Rat
Rat, SD, 1-2 day
Kurachi, M. , Mikuni, M. and Ishizaki, Y.
Extracellular Vesicles from Vascular Endothelial Cells Promote Survival, Proliferation and Motility of Oligodendrocyte Precursor Cells. ,
PLoS ONE
 11 , e0159158 , 2016
Cells
Oligodendrocyte precursor cells
Enzymes
Papain : 16.5 u/ml
Medium
HBSS
Brain
Rat
Rat, SD, E18
Folts, C. , Scott-Hewitt, N. , Proschel, C. , Mayer-Proschel, M. and Noble, M.
Lysosomal Re-acidification Prevents Lysosphingolipid-Induced Lysosomal Impairment and Cellular Toxicity. ,
PLoS Biol
 14 , e1002583 , 2016
Cells
Neurons
Enzymes
Papain : 20 u/ml
Medium
HBSS
Brain
Rat
Rat, P0-P15
Foo, L.
Purification of Rat and Mouse Astrocytes by Immunopanning. ,
Cold Spring Harb. Protoc.
 2013 , 421-32 , 2013
Cells
Astrocytes
Enzymes
Papain : 5 u/ml
Medium
DPBS
Brain
Rat
Rat, SD, neonatal
Kaiser, O. , Aliuos, P. , Wissel, K. , Lenarz, T. , Werner, D. , Reuter, G. , Kral, A. and Warnecke, A.
Dissociated Neurons and Glial Cells Derived from Rat Inferior Colliculi After Digestion with Papain. ,
PLoS ONE
 8 , e80490 , 2013
Cells
Neurons
Enzymes
Papain : 20 u/ml
Medium
Neuro medium
Brain
Rat
Rat, Fisher, 7-21 month
Chen, N. , Newcomb, J. , Garbuzova-Davis, S. , Davis Sanberg, C. , Sanberg, P. and Willing, A.
Human Umbilical Cord Blood Cells Have Trophic Effects on Young and Aging Hippocampal Neurons in Vitro. ,
Aging Dis
 1 , 173 , 2010
Cells
Hippocampal neurons
Enzymes
Papain : 0.2%
Medium
Hibernate A
Brain
Rat
Rat, SD, 19-21 day
Cao, R. , Li, A. , Cho, H. , Lee, B. and Obrietan, K.
Mammalian Target of Rapamycin Signaling Modulates Photic Entrainment of the Suprachiasmatic Circadian Clock. ,
J Neurosci
 30 , 6302 , 2010
Cells
Suprachiasmatic nucleus neurons
Enzymes
Papain : 100 u/ml
Medium
MEM
Brain
Rat
Rat, SD, 7 day
Tanaka, S. , Shaikh, I. , Chiocca, E. and Saeki, Y.
The Gs-Linked Receptor GPR3 Inhibits the Proliferation of Cerebellar Granule Cells During Postnatal Development. ,
PLoS ONE
 4 , e5922 , 2009
Cells
Cerebellar granule neurons
Enzymes
PDS kit : per instructions
Medium
PBS
Brain
Rat
Rat, neonatal
Shigetomi, E. and Khakh, B.
Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes. ,
J Vis Exp
 26 , 1142 , 2009
Cells
Astrocytes
Enzymes
Papain : 20 u/ml
Medium
EBSS
Brain
Rat
Rat, neonatal
Richler Esther, Chaumont Severine, Shigetomi Eiji, Sagasti Alvaro, Khakh Baljit S.
Tracking transmitter-gated P2X cation channel activation in vitro and in vivo ,
Nat Methods
 5 , 87-93 , 2008
Cells
Hippocampal neurons
Enzymes
Papain : 10 u/ml
Medium
EBSS
Brain
Rat
Rat, E18
Jekabsons MB, Nicholls DG.
Bioenergetic analysis of cerebellar granule neurons undergoing apoptosis by potassium/serum deprivation ,
Cell Death Differ
 13 , 1595-610 , 2006
Cells
Hippocampal neurons
Enzymes
Papain : 0.2%
Medium
Hibernate
Brain
Rat
Rat, E19
Khakh Baljit S, Fisher James A, Nashmi Raad, Bowser David N, Lester Henry A.
An angstrom scale interaction between plasma membrane ATP-gated P2X2 and alpha4beta2 nicotinic channels measured with fluorescence resonance energy transfer and total internal reflection fluorescence microscopy ,
J Neurosci
 25 , 6911-20 , 2005
Cells
Hippocampal neuron
Enzymes
Papain : 10 u/ml
Medium
MEM
Brain
Rat
Rat, Wistar, 14 day
Mizoguchi Y, Kanematsu T, Hirata M, Nabekura J.
A rapid increase in the total number of cell surface functional GABAA receptors induced by brain-derived neurotrophic factor in rat visual cortex ,
J Biol Chem
 278 , 44097 , 2003
Cells
Visual cortical
Enzymes
PDS kit : per instructions
Medium
EBSS
Brain
Rat
Rat, SD, E18
Behar, T, Smith, S, Kennedy, R, Mckenzie, J, Maric, I. and Barker, J
GABA(B) receptors mediate motility signals for migrating embryonic cortical cells ,
Cereb Cortex
 11 , 744-53 , 2001
Cells
Cortical cells
Enzymes
Papain : 20 u/ml
Medium
EBSS
Brain
Rat
Rat, wistar, 250-300 g
Brzezinska AK, Gebremedhin D, Chilian WM, Kalyanaraman B, Elliott SJ.
Peroxynitrite reversibly inhibits Ca(2+)-activated K(+) channels in rat cerebral artery smooth muscle cells ,
Am J Physiol Heart Circ Physiol
 278 , H1883 , 2000
Cells
Cerebral artery smooth muscle cells
Enzymes
Papain : 1.5 mg/ml
Collagenase Type 4 : 1.5 mg/ml
Medium
Physiological Salt Solution
Brain
Rat
Rat, SD, E16
Varney MA, Cosford ND, Jachec C, Rao SP, Sacaan A, Lin FF, Bleicher L, Santori EM, Flor PJ, Allgeier H, Gasparini F, Kuhn R, Hess SD, Velicelebi G, Johnson EC.
SIB-1757 and SIB-1893: selective, noncompetitive antagonists of metabotropic glutamate receptor type 5 ,
J Pharmacol Exp Ther
 290 , 170 , 1999
Cells
Cortical neurons
Enzymes
PDS kit : with modifications
Medium
Neurobasal Medium
Brain
Rat
Rat, wistar, neonatal
Boehm S, Betz H.
Somatostatin inhibits excitatory transmission at rat hippocampal synapses via presynaptic receptors ,
J Neurosci
 17 , 4066 , 1997
Cells
Hippocampal cells
Enzymes
Papain : 1 mg/ml
Medium
DMEM
Brain
Rat
Rat, SD, Fisher
Brewer, G.J.
Isolation and culture of adult rat hippocampal neurons of any age ,
J Neurosci Methods
 71 , 143 , 1997
Cells
Hippocampal neurons
Enzymes
Papain : 0.2%
Medium
HibernateA/B27
Brain
Rat
Rat, E18
Naeve GS, Ramakrishnan M, Kramer R, Hevroni D, Citri Y, Theill LE.
Neuritin: a gene induced by neural activity and neurotrophins that promotes neuritogenesis ,
Proc Natl Acad Sci U S A
 94 , 2648-53 , 1997
Cells
Hippocampal and cortical neurons
Enzymes
PDS kit : per instructions
Medium
Neurobasal media
Endothelial
Mouse
Mouse, 2 month
Sabbagh, M. and Nathans, J.
A Genome-wide View of the De-differentiation of Central Nervous System Endothelial Cells in Culture ,
Elife
 , 1 , 2020
Cells
Endothelial, CNS
Enzymes
PDS kit : with modifications
Stemxyme 1 : 0.1%
Medium
EC growth medium
Endothelial
Mouse
Mouse
Daneman, R. , Zhou, L. , Agalliu, D. , Cahoy, J. , Kaushal, A. and Barres, B.
The Mouse Blood-Brain Barrier Transcriptome: a New Resource for Understanding the Development and Function of Brain Endothelial Cells. ,
PLoS ONE
 5 , e13741 , 2010
Cells
Endothelial
Enzymes
Papain : 40 u/ml
Deoxyribonuclease I : 125 u/ml
Medium
DPBS
Epithelial
Mouse
Mouse, 8-12 week
Brann, D. , Tsukahara, T. , Weinreb, C. , Lipovsek, M. , Van den Berge, K. , Gong, B. , Chance, R. , Macaulay, I. , Chou, H. , Fletcher, R. , Das, D. , Street, K. , de Bezieux, H. , Choi, Y. , Risso, D. and Dudoit, S.
Non-Neuronal Expression of SARS-CoV-2 Entry Genes in the Olfactory System Suggests Mechanisms Underlying COVID-19-Associated Anosmia. ,
Sci. Adv.
 6 , 2020
Cells
Nasal epithelium
Enzymes
Papain : 20 u/ml
Medium
EBSS
Epithelial
Mouse
Mouse
Pardo-Saganta, A. , Tata, P. , Law, B. , Saez, B. , Chow, R. , Prabhu, M. , Gridley, T. and Rajagopal, J.
Parent Stem Cells Can Serve as Niches for Their Daughter Cells. ,
Nature
 523 , 597-601 , 2015
Cells
Tracheal epithelial
Enzymes
Papain : 50 u/ml
Medium
See reference
Epithelial
Mouse
Mouse, adult
Gualdoni, S. , Baron, M. , Lakowski, J. , Decembrini, S. , Pearson, R. , Ali, R. and Sowden, J.
Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells. ,
Curr Protoc Stem Cell Biol
 Chapter 1 , Unit 1H.4 , 2011
Cells
Cilary epithelial
Enzymes
PDS kit : per instructions
Medium
EBSS
Epithelial
Rat
Rat, adult
(also hamster)
Heimann, T. and Githens, S.
Rat Pancreatic Duct Epithelium Cultured on a Porous Support Coated with Extracellular Matrix ,
Pancreas
 6 (5) , 514 , 1991
Cells
Interlobular duct fragments
Medium
DMEM/Ham's F-12
Eye
Fish
Fish, zebrafish
Diekmann, H. , Kalbhen, P. and Fischer, D.
Characterization of Optic Nerve Regeneration using Transgenic Zebrafish. ,
Front Cell Neurosci
 9 , 118 , 2015
Cells
Retinal
Enzymes
Papain : 10 u/ml
Medium
L-15
Eye
Fish
Goldfish
Graffe, M. , Zenisek, D. and Taraska, J.
A Marginal Band of Microtubules Transports and Organizes Mitochondria in Retinal Bipolar Synaptic Terminals. ,
J Gen Physiol
 146 , 109-17 , 2015
Cells
Retinal Bipolar
Enzymes
Papain : 30 u/ml
Medium
HEPES
Eye
Human

Human

Menon, M. , Mohammadi, S. , Davila-Velderrain, J. , Goods, B. , Cadwell, T. , Xing, Y. , Stemmer-Rachamimov, A. , Shalek, A. , Love, J. , Kellis, M. and Hafler, B.
Single-cell Transcriptomic Atlas of the Human Retina Identifies Cell Types Associated with Age-related Macular Degeneration. ,
Nat Commun
 10 , 4902 , 2019
Cells
Retinal
Enzymes
PDS kit : see reference
Medium
EBSS
Eye
Human
Human
Xu, N. , Chen, Y. , Dean, K. , Lu, X. , Liu, X. , Wang, W. , Dean, D. , Kaplan, H. , Gao, L. , Dong, F. and Liu, Y.
Sphere-Induced Rejuvenation of Swine and Human Muller Glia Is Primarily Caused by Telomere Elongation. ,
Stem Cells
 35 , 1579-1591 , 2017
Cells
Muller glia
Enzymes
PDS kit : per instructions
Medium
DMEM
Eye
Monkey
Macaques and baboon
Han ,Y. , Jacoby, R. and Wu, S.
Morphological and Electrophysiological Properties of Dissociated Primate Retinal Cells. ,
Brain Res
 875 , 175 , 2000
Cells
Retinal
Enzymes
Papain : 20-40 u/ml
Deoxyribonuclease I : 400 u/ml
Medium
Ames' solution
Eye
Mouse

Mouse, 2 month

Murenu, E. , Pavlou, M. , Richter, L. , Rapti, K. , Just, S. and Cehajic-Kapetanovic, J.
A Universal Protocol for Isolating Retinal ON Bipolar Cells Across Species via Fluorescence-Activated Cell Sorting. , Mol Ther Methods Clin Dev Vol. 20 , 587-600 , 2021
Enzymes
Papain : 20 u/ml
Medium
EBSS
Eye
Mouse
Mouse, 2-4 week
Liu, X. , Tang, L. and Liu Y.
Mouse Muller Cell Isolation and Culture. ,
Bio-protocol
 7 , 2017
Cells
Muller
Enzymes
PDS kit : per instructions
Medium
DMEM
Eye
Mouse
Mouse
Ortin-Martinez, A. , Tsai, E. , Nickerson, P. , Bergeret, M. , Lu, Y. , Smiley, S. , Comanita, L. and Wallace, V.
A Reinterpretation of Cell Transplantation: GFP Transfer From Donor to Host Photoreceptors. ,
Stem Cells
 35 , 932-939 , 2017
Cells
Retinal
Enzymes
Papain : 20 u/ml
Medium
PBS
Eye
Mouse
Mouse
Wohl, S. , Jorstad, N. , Levine, E. and Reh, T.
Muller Glial microRNAs are Required for the Maintenance of Glial Homeostasis and Retinal Architecture. ,
Nat Commun
 8 , 1603 , 2017
Cells
Retinal
Enzymes
Papain : 20 u/ml
Medium
HBSS
Eye
Mouse
Mouse, C57BL/6
Blandford, S. and Baldridge, W.
The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca(2+)]i. ,
Biomed Res Int
 2016 , 8178162 , 2016
Cells
Retinal astrocytes
Enzymes
Papain : 16.5 u/ml
Medium
HBSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.