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Tissue References Search Tool

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Neural
Rat
Rat, Wistar, 4 day
Obradovic Darja, Gronemeyer Hinrich, Lutz Beat, Rein Theo.
Cross-talk of vitamin D and glucocorticoids in hippocampal cells ,
J Neurochem
 96 , 500-9 , 2006
Cells
Hippocampal neurons
Enzymes
PDS kit : per instructions
Medium
Neurobasal A
Neural
Rat
Rat, SD, neonatal
Lacroix-Fralish, M. , Tawfik, V. , Nutile-McMenemy, N. , Harris, B. and Deleo, J.
Differential Regulation of Neuregulin 1 Expression by Progesterone in Astrocytes and Neurons. ,
Neuron Glia Biol
 2 , 227 , 2006
Cells
astrocytes
Enzymes
PDS kit : per instructions
Medium
DMEM
Neural
Rat
Rat, 2 month
Mothe, A. , Kulbatski, I. , Van Bendegem, R. , Lee, L. , Kobayashi, E. , Keating, A. and Tator, C.
Analysis of Green Fluorescent Protein Expression in Transgenic Rats for Tracking Transplanted Neural Stem/Progenitor Cells ,
J Histochem Cytochem
 53(10) , 1215 , 2005
Cells
Spinal cord progenitor cells
Hybrid toxins
Enzymes
PDS kit : see reference
Medium
Neurobasal A
Neural
Rat
Rat, SD, E19
Gavva NR, Tamir R, Qu Y, Klionsky L, Zhang TJ, Immke D, Wang J, Zhu D, Vanderah TW, Porreca F, Doherty EM, Norman MH, Wild KD, Bannon AW, Louis JC, Treanor JJ.
AMG 9810 [(E)-3-(4-t-butylphenyl)-N-(2,3-dihydrobenzo[b][1,4] dioxin-6-yl)acrylamide], a novel vanilloid receptor 1 (TRPV1) antagonist with antihyperalgesic properties ,
J Pharmacol Exp Ther
 313 , 474 , 2005
Cells
Dorsal root ganglia
Enzymes
PDS kit : per instructions
Medium
MEM/Ham's F12
Neural
Rat
Rat, SD, 1-2 day
Floyd Candace L, Gorin Fredric A, Lyeth Bruce G.
Mechanical strain injury increases intracellular sodium and reverses Na+/Ca2+ exchange in cortical astrocytes ,
Glia
 51 , 35-46 , 2005
Cells
Cortical astrocytes
Enzymes
Papain : see reference
Medium
DMEM
Neural
Rat
Rat, SD, male, 5-8 week
Connor, M, Naves, L. and McCleskey, E.
Contrasting Phenotypes of Putative Proprioceptive and Nociceptive Trigeminal Neurons Innervating Jaw Muscle in Rat. ,
Mol Pain
 1 , 31 , 2005
Cells
Trigeminal neurons
Enzymes
Papain : 20 u/ml
Collagenase : 0.3%
Medium
CMF Hanks
Neural
Rat
Rat, Wistar, E15
Moriya-Ito K, Osada T, Ishimatsu Y, Muramoto K, Kobayashi T, Ichikawa M.
Maturation of vomeronasal receptor neurons in vitro by coculture with accessory olfactory bulb neurons ,
Chem Senses
 30 , 111 , 2005
Cells
Vomeronasal receptor neurons
Enzymes
Papain : 0.5 u/ml
Medium
DMEM/F12
Neural
Rat
Rat, fetal
Lovshin JA, Huang Q, Seaberg R, Brubaker PL, Drucker DJ.
Extrahypothalamic expression of the glucagon-like peptide-2 receptor is coupled to reduction of glutamate-induced cell death in cultured hippocampal cells ,
Endocrinology
 145 , 3495 , 2004
Cells
Brainstem and cortical neurons
Enzymes
PDS kit : per instructions
Medium
DMEM
Neural
Rat
Rat, 15 week
Evans J, Sumners C, Moore J, Huentelman MJ, Deng J, Gelband CH. and Shaw G
Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem ,
J Neurophysiol
 87 , 1076 , 2002
Cells
Neurons
Enzymes
Papain : 0.2%
Medium
Hibernate A
Neural
Rat
Rat, SD, male, 270-330 g
Lin CR, Wu PC, Shih HC, Cheng JT, Lu CY, Chou AK, Yang LC.
Intrathecal spinal progenitor cell transplantation for the treatment of neuropathic pain ,
Cell Transplant
 11 , 17 , 2002
Cells
Spinal progenitor cells
Enzymes
PDS kit : with modifications
Medium
Neurobasal medium
Neural
Rat
Rat, Fisher, 8-9 week
Lie DC, Dziewczapolski G, Willhoite AR, Kaspar BK, Shults CW, Gage FH.
The adult substantia nigra contains progenitor cells with neurogenic potential ,
J Neurosci
 22 , 6639 , 2002
Cells
Adult progenitor
Enzymes
Papain : 2.5 u/ml
Deoxyribonuclease I : 250 u/ml
Neutral Protease : 1 u/ml
Medium
DMEM/F-12
Neural
Rat
Rat
Liu, Q. , Kawai, H. and Berg, D.
B-Amyloid Peptide Blocks the Response of a7-Containing Nicotinic Receptors on Hippocampal Neurons ,
PNAS
 98 (8) , 4734 , 2001
Cells
Neurons, hippocampal
Enzymes
Papain : 15 - 20 u/ml
Medium
Eagle's MEM
(see reference)
Neural
Rat
Rat, embryonic 18-19 day
Mabuchi T, Kitagawa K, Kuwabara K, Takasawa K, Ohtsuki T, Xia Z, Storm D, Yanagihara T, Hori M, Matsumoto M.
Phosphorylation of cAMP response element-binding protein in hippocampal neurons as a protective response after exposure to glutamate in vitro and ischemia in vivo ,
J Neurosci
 21 , 9204-13 , 2001
Cells
Hippocampal neurons
Enzymes
PDS kit : see reference
Medium
DMEM
Neural
Rat
Rat, embryonic, day 18
O'Connor, S. , Andreadis, J. , Shaffer, K. , Ma, W. , Pancrazio, J. and Stenger, D.
Immobilization of Neural Cells in Three-Dimensional Matrices for Biosensor Applications ,
Biosensors & Bioelectronics
 14 , 871 , 2000
Cells
Cortical
Medium
Neurobasal medium and DMEM
Neural
Rat
Rat, Wister, postnatal, P1-3 days
Neuhoff, H. , Roeper, J. and Schweizer, M.
Activity-Dependent Formation of Perforated Synapses in Cultured Hippocampal Neurons ,
Eur J Neurosci
 11 , 4241 , 1999
Cells
Neurons, hippocampal
Enzymes
Papain : 20 u/ml
Medium
EBSS
Neural
Rat
Rat, (Long Evans), 2-5 day old
Wilding, T. and Huettner, J.
Activation and Desensitization of Hippocampal Kainate Receptors ,
J Neurosci
 17 (8) , 2713 , 1997
Cells
Neurons, hippocampal
Enzymes
Papain : 20 u/ml
Medium
EBSS
Neural
Rat
Rat, pups, 24-48 h old
Hall, R. and Soderling, T.
Differential Surface Expression and Phosphorylation of the N-Methyl-D-Aspartate Receptor NR1 and NR2 in Cultured Hippocampal Neurons ,
J Biol Chem
 272 (7) , 4135 , 1997
Cells
Neurons, hippocampal
Enzymes
Papain : 20 u/ml
Medium
Harvest buffer
Neural
Rat
Rat, SD, Fisher
Brewer, G.J.
Isolation and culture of adult rat hippocampal neurons of any age ,
J Neurosci Methods
 71 , 143 , 1997
Cells
Hippocampal neurons
Enzymes
Papain : 0.2%
Medium
HibernateA/B27
Neural
Rat
Rat, 1-4 day old
Twitchell, W. , Brown, S. and Mackie, K.
Cannabinoids Inhibit N- and P/Q-Type Calcium Channels in Cultured Rat Hippocampal Neurons ,
J Neurophysiol
 78 , 43 , 1997
Cells
Neurons, hippocampal
Enzymes
Papain : 20 u/ml
Medium
MEM
Neural
Rat
Rat, E18
Liu QY, Schaffner AE, Li YX, Dunlap V, Barker JL.
Upregulation of GABAA current by astrocytes in cultured embryonic rat hippocampal neurons ,
J Neurosci
 16 , 2912-23 , 1996
Cells
Hippocampal neurons
Enzymes
Papain : 20 u/ml
Medium
MEM
Neural
Rat
Rat, 1-6 day
Robertson, S. , Rae, M. , Rowan, E. and Kennedy, C.
Characterization of a P2X-Purinoceptor in Cultured Neurones of the Rat Dorsal Root Ganglia. ,
Br J Pharmacol
 118 , 951 , 1996
Cells
DRG neurons
Enzymes
Papain : 20 u/ml
Collagenase Type 1 : 150 u/ml
Medium
HBSS
Neural
Rat
Rat, postnatal
Hatanaka,H. , Tsukui, H. and Nihonmatsu, I.
Septal Cholinergic Neurons From Postnatal Rat Can Survive In The Dissociate Culture Conditions In The Presence Of Nerve Growth Factor ,
Neurosci Lett
 79 , 85 , 1987
Cells
Septal neurons
Enzymes
Papain : 0.05%
Medium
PBS, CMF
Neural
Rat
Rat, Long Evans, 1-15 days
Huettner, J. and Baughman, R.
Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats ,
J Neurosci
 6 , 3044 , 1986
Cells
Neurons, visual cortex
Enzymes
Papain : 20 u/ml
Medium
BSS
(see reference)
Neural
Rat
Rat, postnatal
Leifer, D. , Lipton, S. , Barnstable, C. and Masland, R.
Monoclonal Antibody to Thy-1 Enhances Regeneration of Processes by Rat Retinal Ganglion Cells in Culture ,
Science
 224 , 303 , 1984
Cells
Ganglion, retina
Enzymes
Papain : 12.5 u/ml
Medium
HBSS w/5 mM HEPES
Neural
Salamander
Salamander, 18-25 cm
Townes-Anderson, E. , MacLeish, P. and Raviola, E.
Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase ,
J Cell Biol
 100 , 175 , 1985
Cells
Retina
Enzymes
Papain : 14 u/ml
Medium
Saline
Neural
Salamander
Salamander (A.tigrinum)
Bader, C. , MacLeish, P. and Schwartz, E.
Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina ,
Proc Natl Acad Sci U S A
 75 , 3507 , 1978
Cells
Photoreceptors, retina
Enzymes
Papain : 0.05%
Medium
(see reference)
Neural
Turtle
Turtle (Pseudemys scripta elegans)
Lam, D.
Biosynthesis of Acetylcholine in Turtle Photoreceptors ,
Proc Natl Acad Sci U S A
 69 , 1987 , 1972
Cells
Retinal
Enzymes
Papain : 0.1% (13.5 u/mg)
Medium
Kreb's Ringer
Organoids
Human

Human

Geest, A. , Jakobs, C. , Ljubikj, T. , Huffels, C. , Luna, M. , Sá, R. , Adolfs, Y. , Wit, M. , Rutten, D. , Kaal, M. , Zwartkruis, M. , Carcolé, M. , Groen, E. , Hol, E. , Basak, O. , Isaacs, A. , Westeneng, H. , Berg, L. , Veldink, J. , Schlegel, D. and Pasterkamp, R.
Molecular Pathology, Developmental Changes, and Synaptic Dysfunction in (pre-) Symptomatic Human C9ORF72-ALS/FTD Cerebral Organoids. , Acta Neuropathol Commun Vol. 12 , 152 , 2024
Cells
Cerebral organoids
Enzymes
Papain : 20 u/ml
Medium
DMEM/F12
Organoids
Human

Human

Lisowski, P. , Lickfett, S. , Rybak-Wolf, A. , Menacho, C. and Le, S.
Mutant Huntingtin Impairs Neurodevelopment in Human Brain Organoids through CHCHD2-mediated Neurometabolic Failure. ,
Nat Commun
 15 , 7027 , 2024
Cells
Brain organoids
Enzymes
Papain : 20 u/ml
Medium
EBSS
Organoids
Human

Human

Tresenrider, A. , Sridhar, A. , Eldred, K. , Cuschieri, S. , Hoffer, D. , Trapnell, C. and Reh, T.
Single-cell Sequencing of Individual Retinal Organoids Reveals Determinants of Cell Fate Heterogeneity. , bioRxiv , 2023
Cells
Retinal Organoids
Enzymes
PDS kit : see reference
Medium
DMEM
Organoids
Human

Human

Schröter, M. , Wang, C. , Terrigno, M. , Hornauer, P. , Huang, Z. , Jagasia, R. and Hierlemann, A.
Functional Imaging of Brain Organoids Using High-Density Microelectrode Arrays. , MRS Bull Vol. 47 , 530-544 , 2022
Cells
Cerebral organoids
Enzymes
PDS kit : see reference
Medium
PBS
Organoids
Human

Human

Uzquiano, A. , Kedaigle, A. , Pigoni, M. , Paulsen, B. , Adiconis, X. , Kim, K. , Faits, T. , Nagaraja, S. , Antón-Bolaños, N. , Gerhardinger, C. , Tucewicz, A. , Murray, E. , Jin, X. , Buenrostro, J. , Chen, F. , Velasco, S. , Regev, A. , Levin, J. and Arlotta, P.
Proper Acquisition of Cell Class Identity in Organoids Allows Definition of Fate Specification Programs of the Human Cerebral Cortex ,
Cell
 185 , 3770-3788.e27 , 2022
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Xue, Y. , Seiler, M. , Tang, W. , Wang, J. , Delgado, J. , McLelland, B. , Nistor, G. , Keirstead, H. and Browne, A.
Retinal Organoids On-a-Chip: a Micro-Millifluidic Bioreactor for Long-term Organoid Maintenance. ,
Lab Chip
 21 , 3361-3377 , 2021
Cells
Stem cell-derived retinal organoids
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Barbar, L. , Rusielewicz, T. , Zimmer, M. , Kalpana, K. and Fossati, V.
Isolation of Human CD49f Astrocytes and iPSC-Based Neurotoxicity Assays. , STAR Protoc Vol. 1 , 100172 , 2020
Cells
Astrocytes
Enzymes
PDS kit : see reference
Medium
EBSS
Organoids
Human

Human

Velasco, S. , Kedaigle, A. , Simmons, S. , Nash, A. , Rocha, M. , Quadrato, G. , Paulsen, B. , Nguyen, L. , Adiconis, X. , Regev, A. , Levin, J. and Arlotta, P.
Individual Brain Organoids Reproducibly Form Cell Diversity of the Human Cerebral Cortex. ,
Nature
 570 , 523-527 , 2019
Cells
Brain organoids
Enzymes
PDS kit : see reference
Medium
PBS
Pancreas
Mouse
Mouse
Githens, S.
Pancreatic Duct Epithelial Cells , Cell & Tissue Culture: Laboratory Procedures Vol. 1 , Doyle, A., Griffiths, J., and Newell, D. , John Wiley and Sons, Ltd. , 12B:12.1 , 1995
Cells
Duct
Enzymes
Papain : 25 u/ml
Medium
DMEM /F-12

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.