Tissue References Search Tool

Rat

Rat, SD, 2-4 day old
(also bovine)

Trifaro, J. , Tang, R. and Novas, M.

Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
26 , 335 , 1990

Cells

Heart

Adrenal chromaffin

Paraneurons

Enzymes

Trypsin : 0.06%

Medium

25mM HEPES buffered Locke's solution, CMF

Rat

Englert, D.F.

An Optical Study of Isolated Rat Adrenal Chromaffin Cells ,
Exp Cell Res
125 , 369 , 1980

Cells

Chromaffin

Enzymes

Trypsin : 0.10%

Medium

Ham's F-12 w/HEPES

Rat

Rat, Wistar-Hanover, 7-12 day

Unsicker, K. , Krisch, B. , Otten, U. and Thoenen, H.

Nerve Growth Factor-Induced Fiber Outgrowth From Isolated Rat Adrenal Chromaffin Cells: Impairment by Glucocorticoids ,
Proc Natl Acad Sci U S A
75 (7) , 3498 , 1978

Cells

Medullary

Enzymes

Trypsin : 0.125%

Medium

HBSS

Rat

Rat, SD, male

Barofsky, A. , Feinstein, M. and Halkerston, I.

Enzymatic and Mechanical Requirements for the Dissociation of Cortical Cells From Rat Adrenal Glands ,
Exp Cell Res
79 , 263 , 1973

Cells

Cortical

Enzymes

Trypsin : 0.25%

Medium

Kreb's Ringer bicarbonate buffer

Bone

Rat

Rat, 2 day

Jeon, J. , Lee, M. and Yang, H.

Differentiated Osteoblasts Derived Decellularized Extracellular Matrix to Promote Osteogenic Differentiation. ,
Biomater Res
22 , 4 , 2018

Cells

Osteoblasts

Enzymes

Collagenase Type 2 : 0.1%

Trypsin : 0.25%

Medium

DMEM

Bone

Rat

Rat, 2-3 day

Taylor, S. , Shah, M. and Orriss, I.

Generation of Rodent and Human Osteoblasts. ,
Bonekey Rep
3 , 585 , 2014

Cells

Osteoblasts

Enzymes

Collagenase Type 2 : 0.2%

Trypsin : 0.25%-1.0%

Medium

DMEM

Rat

Rat, SD, 7 day

Tanaka, S. , Shaikh, I. , Chiocca, E. and Saeki, Y.

The Gs-Linked Receptor GPR3 Inhibits the Proliferation of Cerebellar Granule Cells During Postnatal Development. ,
PLoS ONE
4 , e5922 , 2009

Cells

Cerebellar granule neurons

Enzymes

PDS kit : per instructions

Medium

PBS

Rat

Rat, SD

Akanda, N. , Tofighi, R. , Brask, J. , Tamm, C. , Elinder, F. and Ceccatelli, S.

Voltage-Dependent Anion Channels (VDAC) in the Plasma Membrane Play a Critical Role in Apoptosis in Differentiated Hippocampal Neurons but not in Neural Stem Cells. ,
Cell Cycle
7 , 3225 , 2008

Cells

Hippocampal

Enzymes

Trypsin : 0.1%

Medium

Neurobasal

Rat

Rat, Wistar, 1-3 day

Velasco Myrian, Garcia Esperanza, Onetti Carlos G.

Glucose deprivation activates diversity of potassium channels in cultured rat hippocampal neurons ,
Cell Mol Neurobiol
26 , 307-19 , 2006

Cells

Hippocampal neurons

Enzymes

Trypsin : 0.05%

Medium

DMEM

Rat

Rat, Wistar, 14 day

Mizoguchi Y, Kanematsu T, Hirata M, Nabekura J.

A rapid increase in the total number of cell surface functional GABAA receptors induced by brain-derived neurotrophic factor in rat visual cortex ,
J Biol Chem
278 , 44097 , 2003

Cells

Visual cortical

Enzymes

PDS kit : per instructions

Medium

EBSS

Rat

Rat, SD, E16

Varney MA, Cosford ND, Jachec C, Rao SP, Sacaan A, Lin FF, Bleicher L, Santori EM, Flor PJ, Allgeier H, Gasparini F, Kuhn R, Hess SD, Velicelebi G, Johnson EC.

SIB-1757 and SIB-1893: selective, noncompetitive antagonists of metabotropic glutamate receptor type 5 ,
J Pharmacol Exp Ther
290 , 170 , 1999

Cells

Cortical neurons

Enzymes

PDS kit : with modifications

Medium

Neurobasal Medium

Rat

Rat, E18

Naeve GS, Ramakrishnan M, Kramer R, Hevroni D, Citri Y, Theill LE.

Neuritin: a gene induced by neural activity and neurotrophins that promotes neuritogenesis ,
Proc Natl Acad Sci U S A
94 , 2648-53 , 1997

Cells

Hippocampal and cortical neurons

Enzymes

PDS kit : per instructions

Medium

Neurobasal media

Rat

Rat, newborn

Holzwarth, J. , Glaum, S. and Miller, R.

Activation of Endothelin Receptors by Sarafotoxin Regulates Ca2+ Homeostasis in Cerebellar Astrocytes ,
Glia
5 , 239 , 1992

Cells

Astrocytes

Enzymes

Trypsin : 0.25%

Medium

DMEM, HBSS

Rat

Rat, SD, newborn

Pixley, S.K.

The Olfactory Nerve Contains Two Populations of Glia ,
In Vitro
5 , 269 , 1992

Cells

Glial

Enzymes

Trypsin : 0.0625%

Medium

MEM, sterile

Rat

Rat, 60-72 hours old

Acheson, A. , Barker, P. , Alderson, R. , Miller, F. and Murphy, R.

Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF ,
Neuron
7 , 265 , 1991

Cells

Fibroblasts

Enzymes

Trypsin : 0.2%

Medium

HEPES buffered DMEM

Rat

Rat, SD, 5 day

Mattson, M. and Kater, S.

Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions ,
Brain Res
490 , 110 , 1989

Cells

Hippocampal neurons

Enzymes

Trypsin : 0.2%

Deoxyribonuclease I : 10 ug/ml

Medium

HBSS

Rat

Rat, albino, adult and newborn

Giulian, D. and Baker, T.J.

Characterization of Ameboid Microglia Isolated From Developing Mammalian Brain ,
J Neurosci
6 , 2163 , 1986

Cells

Cerebral cortices

Enzymes

Trypsin : 0.25%

Medium

PBS

Rat

Rat, Wistar, newborn

Goldman, J.E. , Geier, S.S. and Hirano, M.

Differentiation of Astrocytes and Oligodendrocytes From Germinal Matrix Cells in Primary Culture ,
J Neurosci
6 , 52 , 1986

Cells

Germinal matrix

Enzymes

Trypsin : 0.25%

Medium

HBSS

Rat

Rat, fetus, 18 day

Rothman, S.

Synaptic Release of Excitatory Amino Acid Neurotransmitter Mediates Anoxic Neuronal Death ,
J Neurosci
4 (7) , 1884 , 1984

Cells

Hippocampi

Enzymes

Trypsin : 0.1%

Medium

HBSS

Rat

Rat, fetus, 18-20 day

Bartlett, W. and Banker, G.

An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts ,
J Neurosci
4 , 1944 , 1984

Cells

Hippocampal neurons

Enzymes

Trypsin : 0.25%

Medium

HBSS, CMF

Rat

Rat, SD, 19-20 days pregnant

Ahmed, Z. , Walker, P. and Fellows, R.

Properties of Neurons from Dissociated Fetal Rat Brain in Serum-Free Culture ,
J Neurosci
3 (12) , 2448 , 1983

Cells

Neural

Enzymes

Trypsin : 0.25%

Medium

DMEM

Rat

Rat, fetus

Swaiman, K. , Neale, E. , Fitzgerald, S. and Nelson, P.

A Method for Large-scale Production of Mouse Brain Cortical Cultures ,
Brain Res
255 , 361 , 1982

Cells

Cortical

Enzymes

Trypsin : 0.25%

Medium

Puck's D1 (see reference)

Rat

Rat, Wistar, pregnant

Abney, E. , Bartlett, P. and Raff, M.

Astrocytes, Ependymal Cells, and Oligodendrocytes Develop on Schedule in Dissociated Cell Cultures of Embryonic Rat Brain ,
Dev Biol
83 , 301 , 1981

Cells

Glial

Enzymes

Trypsin : 0.05%

Medium

Eagle's MEM/DMEM

Rat

Rat, Wistar, adult, 170 g

Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.

Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
129 , 261 , 1979

Cells

Endothelial

Enzymes

Trypsin : 0.5%

Medium

BSS

Rat

Rat, Holtzmann, 18 day

Banker, G. and Cowan, M.

Rat Hippocampal Neurons in Dispersed Cell Culture ,
Brain Res
126 , 397 , 1977

Cells

Hippocampal neurons

Enzymes

Trypsin : 0.1%

Medium

HBSS

Rat

Rat, SD, 2 days

Rowe, V. , Neale, E. , Avins, L. , Guroff, G. and Schrier, B.

Pineal gland cells in culture. Morphology, Biochemistry, Differentiation, and co-culture with sympathetic neurons ,
Exp Cell Res
104 , 345 , 1977

Cells

Pineal

Enzymes

Trypsin : 0.25%

Medium

DMEM, MEM

Cartilage

Rat

Mei, L. , Shen, B. , Ling, P. , Liu, S. , Xue, J. , Liu, F. , Shao, H. , Chen, J. , Ma, A. and Liu, X.

Culture-expanded Allogenic Adipose Tissue-derived Stem Cells Attenuate Cartilage Degeneration in an Experimental Rat Osteoarthritis Model. ,
PLoS ONE
12 , e0176107 , 2017

Cells

Chondrocytes

Enzymes

Collagenase Type 2 : 0.15%

Trypsin : 0.25%

Medium

DMEM/F12

Cartilage

Rat

Rat, SD, young, 100-120 g

Shimomura, Y. , Yoneda, T. and Suzuki, F.

Osteogenesis by Chondrocytes from Growth Cartilage of Rat Rib ,
Calcif Tissue Res
19 , 179 , 1975

Cells

Chondrocytes

Enzymes

Trypsin : 0.2%

Medium

Ham's F-12 medium

Endothelial

Rat

Rat, 300 G, and pig, 30-40 Kg

Merrilees, M.J. and Scott, L.

Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
39 , 147 , 1981

Cells

Endothelial

Thoracic aorta

Enzymes

Collagenase Type 4 : 0.025%

Trypsin : 0.05%

Medium

Medium 199 and 0.01M EDTA

Endothelial

Rat

Rat, Wistar, adult, 170 g

Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.

Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
129 , 261 , 1979

Cells

Endothelial

Enzymes

Trypsin : 0.5%

Medium

BSS

Rat

Rat, SD, adult, male, 8-10 weeks old

Abou-Haila, A. and Tulsiani, D.

Acid Glycohydrolases in Rat Spermatocytes, Spermatids and Spermatozoa: Enzyme Activities, Biosynthesis and Immunolocalization ,
Biol Proced Online
3 (1) , 35 , 2001

Cells

Seminiferous tubules

Enzymes

Trypsin : 0.05%

Medium

Krebs-Ringer bicarbonate buffer

(see reference)

Rat

Rodent, various (see reference)

Robinson, C. and Wu, R.

Culture of Conducting Airway Epithelial Cells in Serum-Free Medium ,
J Tiss Cul Meth
13 , 95 , 1991

Cells

Epithelial

Enzymes

Trypsin : 0.2%

Medium

MEM, PBS

Rat

Rat, Wistar, neonatal

Jassal, D. , Han, R. , Caniggia, I. , Post, M. and Tanswell, A.

Growth of Distal Fetal Rat Lung Epithelial Cells in a Defined Serum-Free Medium ,
In Vitro Cell Dev Biol
27A , 625 , 1991

Cells

Epithelial

Enzymes

Trypsin : 0.1%

Medium

HBSS

Rat

Rat, Fischer, Lewis and SD, male, 10-18 months

Herring, A. , Raychaudhuri, R. , Kelley, S. and Iybe, P.

Repeated Establishment of Diploid Epithelial Cell Cultures from Normal and Partially Hepatectomized Rats ,
In Vitro
19 , 576 , 1983

Cells

Epithelial

Enzymes

Trypsin : 0.05%

Medium

HBSS CMF