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Tissue References Search Tool

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Eye
Fish
Fish, zebrafish
Diekmann, H. , Kalbhen, P. and Fischer, D.
Characterization of Optic Nerve Regeneration using Transgenic Zebrafish. ,
Front Cell Neurosci
 9 , 118 , 2015
Cells
Retinal
Enzymes
Papain : 10 u/ml
Medium
L-15
Eye
Fish
Goldfish
Graffe, M. , Zenisek, D. and Taraska, J.
A Marginal Band of Microtubules Transports and Organizes Mitochondria in Retinal Bipolar Synaptic Terminals. ,
J Gen Physiol
 146 , 109-17 , 2015
Cells
Retinal Bipolar
Enzymes
Papain : 30 u/ml
Medium
HEPES
Eye
Monkey
Macaques and baboon
Han ,Y. , Jacoby, R. and Wu, S.
Morphological and Electrophysiological Properties of Dissociated Primate Retinal Cells. ,
Brain Res
 875 , 175 , 2000
Cells
Retinal
Enzymes
Papain : 20-40 u/ml
Deoxyribonuclease I : 400 u/ml
Medium
Ames' solution
Eye
Mouse

Mouse, 2 month

Murenu, E. , Pavlou, M. , Richter, L. , Rapti, K. , Just, S. and Cehajic-Kapetanovic, J.
A Universal Protocol for Isolating Retinal ON Bipolar Cells Across Species via Fluorescence-Activated Cell Sorting. , Mol Ther Methods Clin Dev Vol. 20 , 587-600 , 2021
Enzymes
Papain : 20 u/ml
Medium
EBSS
Eye
Mouse
Mouse
Ortin-Martinez, A. , Tsai, E. , Nickerson, P. , Bergeret, M. , Lu, Y. , Smiley, S. , Comanita, L. and Wallace, V.
A Reinterpretation of Cell Transplantation: GFP Transfer From Donor to Host Photoreceptors. ,
Stem Cells
 35 , 932-939 , 2017
Cells
Retinal
Enzymes
Papain : 20 u/ml
Medium
PBS
Eye
Mouse
Mouse
Wohl, S. , Jorstad, N. , Levine, E. and Reh, T.
Muller Glial microRNAs are Required for the Maintenance of Glial Homeostasis and Retinal Architecture. ,
Nat Commun
 8 , 1603 , 2017
Cells
Retinal
Enzymes
Papain : 20 u/ml
Medium
HBSS
Eye
Mouse
Mouse, C57BL/6
Blandford, S. and Baldridge, W.
The Effect of Glutamate Receptor Agonists on Mouse Retinal Astrocyte [Ca(2+)]i. ,
Biomed Res Int
 2016 , 8178162 , 2016
Cells
Retinal astrocytes
Enzymes
Papain : 16.5 u/ml
Medium
HBSS
Eye
Mouse
Mouse, embryonic and P0
Dvoriantchikova, G. , Perea-Martinez, I. , Pappas, S. , Barry, A. , Danek, D. , Dvoriantchikova, X. , Pelaez, D. and Ivanov, D.
Molecular Characterization of Notch1 Positive Progenitor Cells in the Developing Retina. ,
PLoS ONE
 10 , e0131054 , 2015
Cells
Retinal
Enzymes
Papain : 8 u/ml
Deoxyribonuclease I : 124 u/ml
Medium
DPBS
Eye
Mouse
mouse, adult
Jadhav AP, Cho SH. and Cepko CL
Notch activity permits retinal cells to progress through multiple progenitor states and acquire a stem cell property ,
Proc Natl Acad Sci U S A
 103 , 18998 , 2006
Cells
Retinal
Enzymes
Papain : 50 u/ml
Medium
DMEM/F-12
Eye
Mouse
Mouse, 2-5 month
Maxeiner Stephan, Dedek Karin, Janssen-Bienhold Ulrike, Ammermuller Josef, Brune Hendrik, Kirsch Taryn, Pieper Mario, Degen Joachim, Kruger Olaf, Willecke Klaus, Weiler Reto.
Deletion of connexin45 in mouse retinal neurons disrupts the rod/cone signaling pathway between AII amacrine and ON cone bipolar cells and leads to impaired visual transmission ,
J Neurosci
 25 , 566-76 , 2005
Cells
Mouse retinal and bipolar
Enzymes
Papain : 20 u/ml
Deoxyribonuclease I : 200 u/ml
Medium
HBSS
Eye
Rabbit
Rabbit, adult
Brockway LM, Zhou Z-H, Bubien JK, Jovov B, Benos DJ, Keyser KT.
Rabbit retinal neurons and glia express a variety of ENaC/DEG subunits ,
Am J Physiol Cell Physiol
 283 , C126-34 , 2002
Cells
Retinal neurons
Enzymes
Papain : 26 u/ml
Medium
DMEM
Eye
Rat

Rat, SD, 1-2 day

Lian, W. , Hu, X. , Zhang, J. , Wu, Y. , Zhao, N. , Ma, H. , He, H. and Lu, Q.
Fucoxanthin Protects Retinal Ganglion Cells and Promotes Parkin-mediated Mitophagy against Glutamate Excitotoxicity. ,
Neuroreport
 34 , 385-394 , 2023
Cells
Retinal ganglion
Enzymes
Papain : 0.5%
Medium
MEM
Eye
Rat
Rat, Wister, 3-30 wk
Cameron, M. , Kekesi, O. , Morley, J. , Tapson, J. , Breen, P. , Van Schaik, A. and Buskila, Y.
Calcium Imaging of AM Dyes Following Prolonged Incubation in Acute Neuronal Tissue. ,
PLoS ONE
 11 , e0155468 , 2016
Cells
Retinal
Enzymes
Papain : 30 u/ml
Medium
EBSS
Eye
Rat
Rat, SD, 180-220g
Ma, J. , Yu, W. , Wang, Y. , Cao, G. , Cai, S. , Chen, X. , Yan, N. , Yuan, Y. , Zeng, H. , Fleenor, D. , Liu, X. and Pang, I.
Neuroprotective Effects of C-type Natriuretic Peptide on Rat Retinal Ganglion Cells. ,
Inv Ophthal Visual Sci
 51 , 3544-53 , 2010
Cells
Retinal ganglion
Enzymes
Papain : 0.2%
Medium
Neurobasal
Eye
Rat
Rat, SD
Jing, S. , Wen, D. , Yu, Y. , Holst, P. , Luo, Y. , Fang, M. , Tamir, R. , Antonio, L. , Hu, Z. and et al.
GDNF-Induced Activation of the Ret Protein Tyrosine Kinase is Mediated by GDNFR-a, a Novel Receptor for GDNF ,
Cell
 85 , 1113 , 1996
Cells
Retina
Enzymes
Papain : 120 U
Medium
HBSS, PBS
Eye
Rat
Rat, Long Evans, 1-15 days
Huettner, J. and Baughman, R.
Primary Culture of Identified Neurons From the Visual Cortex of Postnatal Rats ,
J Neurosci
 6 , 3044 , 1986
Cells
Neurons, visual cortex
Enzymes
Papain : 20 u/ml
Medium
BSS
(see reference)
Eye
Salamander
Salamander
Clarke, R, Wang, J. and Townes-Anderson, E.
Using Laser Tweezers For Manipulating Isolated Neurons In Vitro. ,
J Vis Exp
 19 , 911 , 2008
Cells
Neurons
Enzymes
Papain : 14 u/ml
Medium
Ringers
Eye
Salamander
Salamander, 18-25 cm
Townes-Anderson, E. , MacLeish, P. and Raviola, E.
Rod Cells Dissociated from Mature Salamander Retina: Ultrastruture and Uptake of Horseradish Peroxidase ,
J Cell Biol
 100 , 175 , 1985
Cells
Retina
Enzymes
Papain : 14 u/ml
Medium
Saline
Eye
Salamander
Salamander (A. tigrinum)
Bader, C. , MacLeish, P. and Schwartz, E.
Responses to Light of Solitary Rod Photoreceptors Isolated From Tiger Salamander Retina ,
Proc Natl Acad Sci U S A
 75 , 3507 , 1978
Cells
Photoreceptors, retina
Enzymes
Papain : 0.05%
Medium
(see reference)
Eye
Turtle
Turtle (Pseudemys scripta elegans)
Lam, D.
Biosynthesis of Acetylcholine in Turtle Photoreceptors ,
Proc Natl Acad Sci U S A
 69 , 1987 , 1972
Cells
Retinal
Enzymes
Papain : 0.1% (13.5 u/mg)
Medium
Kreb's Ringer
Miscellaneous
Human

Human

Yoon, S. , Elahi, L. , Pașca, A. , Marton, R. , Gordon, A. , Revah, O. , Miura, Y. , Walczak, E. , Holdgate, G. , Fan, H. , Huguenard, J. , Geschwind, D. and Pașca, S.
Reliability of Human Cortical Organoid Generation. ,
Nat Methods
 16 , 75-78 , 2019
Cells
Cortical spheroids
Enzymes
Papain : 40 u/ml
Medium
EBSS
Miscellaneous
Invertebrate

Octopus vulgaris

Maselli, V. , Xu, F. , Syed, N. , Polese, G. and Cosmo, A.
A Novel Approach to Primary Cell Culture for Octopus vulgaris Neurons. ,
Front Physiol
 9 , 220 , 2018
Cells
Neurons
Enzymes
Papain : 0.1%
Trypsin : 0.1%
Medium
L15-ASW
Miscellaneous
Invertebrate
Squid
Raman, M. and Mathew, S.
Study of Chemical Properties and Evaluation of Collagen in Mantle, Epidermal Connective Tissue and Tentacle of Indian Squid, Loligo duvauceli Orbigny. ,
J Food Sci Technol
 51 , 1509-16 , 2014
Cells
Mantle
Enzymes
Collagenase : see reference
Papain : see reference
Medium
Phosphate buffer
Miscellaneous
Mouse
Mouse, 8-12 week
Atta, H. , Gheinani, A. , Wacker, A. , Heshmati, Y. , Bigger-Allen, A. , Lambrinos, G. , Gao, Y. , Bielenberg, D. and Adam, R.
A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury. ,
J Vis Exp
 , 2020
Cells
Urinary bladder
Enzymes
Collagenase Type 1 : 50 u/ml
Collagenase Type 3 : 40 u/ml
Papain : 45 u/ml
Collagenase Type 6 : 20 u/ml
Medium
Tyrode solution
Miscellaneous
Mouse
Mouse
Kim, Y. , Wang, S. , Tymanskyj, S. , Ma, L. , Tao, H. and Zhang, L.
Dcc Mediates Functional Assembly of Peripheral Auditory Circuits. ,
Sci Rep
 6 , 23799 , 2016
Cells
Cochlear
Enzymes
Papain : 20 u/ml
Trypsin : 0.05%
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.