Tissue References Search Tool

Human

Yoon, S. , Elahi, L. , Pașca, A. , Marton, R. , Gordon, A. , Revah, O. , Miura, Y. , Walczak, E. , Holdgate, G. , Fan, H. , Huguenard, J. , Geschwind, D. and Pașca, S.

Reliability of Human Cortical Organoid Generation. ,
Nat Methods
16 , 75-78 , 2019

Cells

Cortical spheroids

Enzymes

Papain : 40 u/ml

Medium

EBSS

Miscellaneous

Invertebrate

Octopus vulgaris

Maselli, V. , Xu, F. , Syed, N. , Polese, G. and Cosmo, A.

A Novel Approach to Primary Cell Culture for Octopus vulgaris Neurons. ,
Front Physiol
9 , 220 , 2018

Cells

Neurons

Enzymes

Papain : 0.1%

Collagenase Type 4 : 0.4%

Trypsin : 0.1%

Medium

L15-ASW

Miscellaneous

Invertebrate

Squid

Raman, M. and Mathew, S.

Study of Chemical Properties and Evaluation of Collagen in Mantle, Epidermal Connective Tissue and Tentacle of Indian Squid, Loligo duvauceli Orbigny. ,
J Food Sci Technol
51 , 1509-16 , 2014

Cells

Mantle

Enzymes

Collagenase : see reference

Papain : see reference

Medium

Phosphate buffer

Miscellaneous

Mouse

Mouse, 8-12 week

Atta, H. , Gheinani, A. , Wacker, A. , Heshmati, Y. , Bigger-Allen, A. , Lambrinos, G. , Gao, Y. , Bielenberg, D. and Adam, R.

A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury. ,
J Vis Exp
, 2020

Cells

Urinary bladder

Enzymes

Collagenase Type 1 : 50 u/ml

Collagenase Type 3 : 40 u/ml

Papain : 45 u/ml

Deoxyribonuclease I : 4 u/ml

Collagenase Type 6 : 20 u/ml

Medium

Tyrode solution

Miscellaneous

Mouse

Kim, Y. , Wang, S. , Tymanskyj, S. , Ma, L. , Tao, H. and Zhang, L.

Dcc Mediates Functional Assembly of Peripheral Auditory Circuits. ,
Sci Rep
6 , 23799 , 2016

Cells

Cochlear

Enzymes

Papain : 20 u/ml

Trypsin : 0.05%

Medium

DMEM

Muscle

Guinea-Pig

Guinea pig, 2-4 wk old, male, female

Firth, T. , Mawe, G. and Nelson, M.

Pharmacology and Modulation of KATP Channels by Protein Kinase C and Phosphatates in Gallbladder Smooth Muscle ,
Am J Physiol Cell Physiol
278 , C1031 , 2000

Cells

Smooth muscle

Gallbladder

Enzymes

Papain : 0.1%

Medium

Krebs solution

Muscle

Guinea-Pig

Guinea-pig, adult, 250-350 g

Jennings, L. , Xu, Q. , Firth, T. , Nelson, M. and Mawe, G.

Cholesterol Inhibits Spontaneous Action Potentials and Calcium Currents in Guinea Pig Gallbladder Smooth Muscle ,
Am J Physiol
277 , G1017 , 1999

Cells

Smooth muscle

Gallbladder

Enzymes

Papain : 0.1%

Medium

NaCl, sodium glutamate, MgCl, KCl, glucose, Kreb's, and HEPES

Muscle

Human

Malysz, J. , Rovner, E. , Wake, R. and Petkov, G.

Preparation and Utilization of Freshly Isolated Human Detrusor Smooth Muscle Cells for Characterization of 9-Phenanthrol-Sensitive Cation Currents. ,
J Vis Exp
, 2020

Cells

Bladder smooth muscle

Enzymes

Collagenase Type 2 : 0.1-0.2%

Papain : 0.1-0.2%

Medium

Digestion solution (see reference)

Muscle

Ovine

Sheep, adult and neonatal

Driska S. , Laudadio R. , Wolfson M. and Shaffer T.

A Method for Isolating Adult and Neonatal Airway Smooth Muscle Cells and Measuring Shortening Velocity ,
J Appl Physiol
86(1) , 427 , 1999

Cells

Tracheal smooth muscle cells

Enzymes

Papain : 0.2%

Deoxyribonuclease I : 0.1%

Medium

MOPS-PSS

Muscle

Rat

Rat, SD, 250 g

Jaggar JH.

Intravascular pressure regulates local and global Ca(2+) signaling in cerebral artery smooth muscle cells ,
Am J Physiol Cell Physiol
281 , C439-48 , 2001

Cells

Arterial smooth muscle

Enzymes

Papain : 0.03%

Collagenase : 0.1%

Medium

see reference

Muscle

Rat

Rat, SHRs and WKY, male, 10 - 14 weeks

Bolzon, B. and Cheung, D.

Isolation and Characterization of Single Vascular Smooth Muscle Cells From Spontaneously Hypertensive Rats ,
Hypertension
14 , 137 , 1989

Cells

Smooth muscle, tail arteries

Enzymes

Papain : 0.1%

Medium

HEPES buffer

(see reference)

Tissue Tables (references, grouped by tissue type and species)

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Tissue References Search Tool

Tissue Tables (references, grouped by tissue type and species)