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Tissue References Search Tool

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Adipose/Fat
Rat
Rat, SD, neonatal
Liu, Z. , Wang, H. , Zhang, Y. , Zhou, J. , Lin, Q. , Wang, Y. , Duan, C. , Wu, K. and Wang, C.
Efficient Isolation of Cardiac Stem Cells from Brown Adipose. ,
J Biomed Biotechnol
 2010 , 104296 , 2010
Cells
Brown adipocytes
Enzymes
Medium
PBS
Adrenal
Rat
Rat, SD, 2-4 day old
(also bovine)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Adrenal
Rat
Rat
Englert, D.F.
An Optical Study of Isolated Rat Adrenal Chromaffin Cells ,
Exp Cell Res
 125 , 369 , 1980
Cells
Chromaffin
Enzymes
Trypsin : 0.10%
Medium
Ham's F-12 w/HEPES
Adrenal
Rat
Rat, Wistar-Hanover, 7-12 day
Unsicker, K. , Krisch, B. , Otten, U. and Thoenen, H.
Nerve Growth Factor-Induced Fiber Outgrowth From Isolated Rat Adrenal Chromaffin Cells: Impairment by Glucocorticoids ,
Proc Natl Acad Sci U S A
 75 (7) , 3498 , 1978
Cells
Medullary
Enzymes
Trypsin : 0.125%
Medium
HBSS
Adrenal
Rat
Rat, SD, male
Barofsky, A. , Feinstein, M. and Halkerston, I.
Enzymatic and Mechanical Requirements for the Dissociation of Cortical Cells From Rat Adrenal Glands ,
Exp Cell Res
 79 , 263 , 1973
Cells
Cortical
Enzymes
Trypsin : 0.25%
Medium
Kreb's Ringer bicarbonate buffer
Bone
Chicken
Chick, day old
Gay, C. , Lloyd, Q. and Gilman, V.
Characteristics and Culture of Osteoblasts Derived From Avian Long Bone ,
In Vitro Cell Dev Biol
 30A , 379 , 1994
Cells
Osteoblasts
Enzymes
Trypsin : 0.03%
Medium
DMEM
Bone
Chicken
Chick, Peterson/Arbor Acre, male, 4 weeks old (Gallus domesticus)
Rosselot, G. , Reginato, A.M. and Leach, R.M.
Development of a Serum-Free System to Study the Effect of Growth Hormone and Insulinlike Growth Factor-1 on Cultured Postembryonic Growth Plate Chondrocytes ,
In Vitro Cell Dev Biol
 28A , 235 , 1992
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Medium
Ham's F12
Bone
Chicken
Chick, embryo
Schiltz, J.R. , Mayne R. and Holtzer, H.
The Synthesis of Collagen and Glycosaminoglycans by Dedifferentiated Chondroblasts in Culture ,
Differentiation
 1 , 97 , 1973
Cells
Vertebrae chondroblasts
Enzymes
Trypsin : 0.25%
Medium
Simm's, CMF
Bone
Rat
Rat, 2 day
Jeon, J. , Lee, M. and Yang, H.
Differentiated Osteoblasts Derived Decellularized Extracellular Matrix to Promote Osteogenic Differentiation. ,
Biomater Res
 22 , 4 , 2018
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Bone
Rat
Rat, 2-3 day
Taylor, S. , Shah, M. and Orriss, I.
Generation of Rodent and Human Osteoblasts. ,
Bonekey Rep
 3 , 585 , 2014
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%-1.0%
Medium
DMEM
Brain
Rat
Rat, SD
Akanda, N. , Tofighi, R. , Brask, J. , Tamm, C. , Elinder, F. and Ceccatelli, S.
Voltage-Dependent Anion Channels (VDAC) in the Plasma Membrane Play a Critical Role in Apoptosis in Differentiated Hippocampal Neurons but not in Neural Stem Cells. ,
Cell Cycle
 7 , 3225 , 2008
Cells
Hippocampal
Enzymes
Trypsin : 0.1%
Medium
Neurobasal
Brain
Rat
Rat, Wistar, 1-3 day
Velasco Myrian, Garci­a Esperanza, Onetti Carlos G.
Glucose deprivation activates diversity of potassium channels in cultured rat hippocampal neurons ,
Cell Mol Neurobiol
 26 , 307-19 , 2006
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.05%
Medium
DMEM
Brain
Rat
Rat, newborn
Holzwarth, J. , Glaum, S. and Miller, R.
Activation of Endothelin Receptors by Sarafotoxin Regulates Ca2+ Homeostasis in Cerebellar Astrocytes ,
Glia
 5 , 239 , 1992
Cells
Astrocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM, HBSS
Brain
Rat
Rat, SD, newborn
Pixley, S.K.
The Olfactory Nerve Contains Two Populations of Glia ,
In Vitro
 5 , 269 , 1992
Cells
Glial
Enzymes
Trypsin : 0.0625%
Medium
MEM, sterile
Brain
Rat
Rat, 60-72 hours old
Acheson, A. , Barker, P. , Alderson, R. , Miller, F. and Murphy, R.
Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF ,
Neuron
 7 , 265 , 1991
Cells
Fibroblasts
Enzymes
Trypsin : 0.2%
Medium
HEPES buffered DMEM
Brain
Rat
Rat, SD, 5 day
Mattson, M. and Kater, S.
Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions ,
Brain Res
 490 , 110 , 1989
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.2%
Deoxyribonuclease I : 10 ug/ml
Medium
HBSS
Brain
Rat
Rat, albino, adult and newborn
Giulian, D. and Baker, T.J.
Characterization of Ameboid Microglia Isolated From Developing Mammalian Brain ,
J Neurosci
 6 , 2163 , 1986
Cells
Cerebral cortices
Enzymes
Trypsin : 0.25%
Medium
PBS
Brain
Rat
Rat, Wistar, newborn
Goldman, J.E. , Geier, S.S. and Hirano, M.
Differentiation of Astrocytes and Oligodendrocytes From Germinal Matrix Cells in Primary Culture ,
J Neurosci
 6 , 52 , 1986
Cells
Germinal matrix
Enzymes
Trypsin : 0.25%
Medium
HBSS
Brain
Rat
Rat, fetus, 18 day
Rothman, S.
Synaptic Release of Excitatory Amino Acid Neurotransmitter Mediates Anoxic Neuronal Death ,
J Neurosci
 4 (7) , 1884 , 1984
Cells
Hippocampi
Enzymes
Trypsin : 0.1%
Medium
HBSS
Brain
Rat
Rat, fetus, 18-20 day
Bartlett, W. and Banker, G.
An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts ,
J Neurosci
 4 , 1944 , 1984
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Brain
Rat
Rat, SD, 19-20 days pregnant
Ahmed, Z. , Walker, P. and Fellows, R.
Properties of Neurons from Dissociated Fetal Rat Brain in Serum-Free Culture ,
J Neurosci
 3 (12) , 2448 , 1983
Cells
Neural
Enzymes
Trypsin : 0.25%
Medium
DMEM
Brain
Rat
Rat, fetus
Swaiman, K. , Neale, E. , Fitzgerald, S. and Nelson, P.
A Method for Large-scale Production of Mouse Brain Cortical Cultures ,
Brain Res
 255 , 361 , 1982
Cells
Cortical
Enzymes
Trypsin : 0.25%
Medium
Puck's D1 (see reference)
Brain
Rat
Rat, Wistar, pregnant
Abney, E. , Bartlett, P. and Raff, M.
Astrocytes, Ependymal Cells, and Oligodendrocytes Develop on Schedule in Dissociated Cell Cultures of Embryonic Rat Brain ,
Dev Biol
 83 , 301 , 1981
Cells
Glial
Enzymes
Trypsin : 0.05%
Medium
Eagle's MEM/DMEM
Brain
Rat
Rat, Wistar, adult, 170 g
Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.
Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
 129 , 261 , 1979
Cells
Endothelial
Enzymes
Trypsin : 0.5%
Medium
BSS
Brain
Rat
Rat, Holtzmann, 18 day
Banker, G. and Cowan, M.
Rat Hippocampal Neurons in Dispersed Cell Culture ,
Brain Res
 126 , 397 , 1977
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.1%
Medium
HBSS
Brain
Rat
Rat, SD, 2 days
Rowe, V. , Neale, E. , Avins, L. , Guroff, G. and Schrier, B.
Pineal gland cells in culture. Morphology, Biochemistry, Differentiation, and co-culture with sympathetic neurons ,
Exp Cell Res
 104 , 345 , 1977
Cells
Pineal
Enzymes
Trypsin : 0.25%
Medium
DMEM, MEM
Cartilage
Chicken
Chick embryos, HH stage
Wong, M. and Tuan, R.
Nuserum, A Synthetic Serum Replacement, Supports Chondrogenesis of Embryonic Chick Limb Bud Mesenchymal Cells in Micromass Culture ,
In Vitro Cell Dev Biol
 29A , 917 , 1993
Cells
Mesenchymal
Enzymes
Trypsin : 0.1%
Medium
DMEM
(see reference)
Cartilage
Chicken
Chicken, broiler strain, 8-10 weeks
Genge, B. , Wu, L. and Wuthier, R.
Differential Fractionation of Matrix Vesicle Proteins: Further Characterization of the Acidic Phospholipid-dependent Ca2+-Binding Proteins ,
J Biol Chem
 265 , 4703 , 1990
Cells
Matrix vesicles
Epiphyseal growth plate
Enzymes
Trypsin : 0.1%
Medium
Tris-buffered saline
Cartilage
Chicken
Chicken, broiler strain, 8-10 week
Genge, B.R. , Wu, L.N.Y. and Wuthier, R.E.
Identification of Phospholipid-dependent Calcium-binding Proteins as Constituents of Matrix Vesicles ,
J Biol Chem
 264 , 10917 , 1989
Cells
Matrix vesicles
Enzymes
Trypsin : 0.1%
Medium
(see reference)
Cartilage
Chicken
Chick embryos, White Leghorn, 19 day old
Gionti, E. , Capasso, O. and Cancedda, R.
The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions in Vitro ,
J Biol Chem
 258 (11) , 7190 , 1983
Cells
Fibroblasts
Epithelial-like
Enzymes
Trypsin : 0.25%
Medium
E 199 medium
Cartilage
Chicken
Chick embryos, 19 day old
Capasso, O. , Gionti, E. , Pontarelli, G. , Ambesi-Impiobato, F. , Nitsch, L. , Tajana, G. and Cancedda, R.
The Culture of Chick Embryo Chondrocytes and the Control of Their Differentiated Functions In Vitro ,
Exp Cell Res
 142 , 197 , 1982
Cells
Chondrocytes
Enzymes
Trypsin : 0.75%
Medium
Coon's modified F-12
Cartilage
Chicken
Chick embryos, White Leghorn
Ahrens, P. , Solursh, M. and Reiter, R.
Stage-Related Capacity for Limb Chondrogenesis in Cell Culture ,
Dev Biol
 60 , 69 , 1977
Cells
Wing buds
Enzymes
Trypsin : 0.1%
Medium
Saline G
Cartilage
Rat
Rat
Mei, L. , Shen, B. , Ling, P. , Liu, S. , Xue, J. , Liu, F. , Shao, H. , Chen, J. , Ma, A. and Liu, X.
Culture-expanded Allogenic Adipose Tissue-derived Stem Cells Attenuate Cartilage Degeneration in an Experimental Rat Osteoarthritis Model. ,
PLoS ONE
 12 , e0176107 , 2017
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Cartilage
Rat
Rat, SD, young, 100-120 g
Shimomura, Y. , Yoneda, T. and Suzuki, F.
Osteogenesis by Chondrocytes from Growth Cartilage of Rat Rib ,
Calcif Tissue Res
 19 , 179 , 1975
Cells
Chondrocytes
Enzymes
Trypsin : 0.2%
Medium
Ham's F-12 medium
Endothelial
Rat
Rat, 300 G, and pig, 30-40 Kg
Merrilees, M.J. and Scott, L.
Interaction of Aortic Endothelial and Smooth Muscle Cells in Culture ,
Atherosclerosis
 39 , 147 , 1981
Cells
Endothelial
Thoracic aorta
Enzymes
Trypsin : 0.05%
Medium
Medium 199 and 0.01M EDTA
Endothelial
Rat
Rat, Wistar, adult, 170 g
Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.
Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
 129 , 261 , 1979
Cells
Endothelial
Enzymes
Trypsin : 0.5%
Medium
BSS

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.