For our international customers, please be advised that orders cannot be placed through our website by customers in countries with International Distributor representation.

Quick Order

Tissue References Search Tool

Search
Adipose/Fat
Rat
Rat, SD, neonatal
Liu, Z. , Wang, H. , Zhang, Y. , Zhou, J. , Lin, Q. , Wang, Y. , Duan, C. , Wu, K. and Wang, C.
Efficient Isolation of Cardiac Stem Cells from Brown Adipose. ,
J Biomed Biotechnol
 2010 , 104296 , 2010
Cells
Brown adipocytes
Enzymes
Medium
PBS
Adrenal
Human
Human, adult
Tischler, A. , DeLellis, R. , Bailes, B. , Nunnemacher, G.,Carabba, V. and Wolfe, H.
Nerve Growth Factor-Induced Neurite Outgrowth from Normal Human Chromaffin Cells ,
Lab Invest
 43 , 399 , 1980
Cells
Chromaffin
Enzymes
Trypsin : 0.25%
Medium
Eagle's MEM
Adrenal
Rat
Rat, SD, 2-4 day old
(also bovine)
Trifaro, J. , Tang, R. and Novas, M.
Monolayer Co-Culture of Rat Heart Cells and Bovine Adrenal Chromaffin Paraneurons ,
In Vitro Cell Dev Biol
 26 , 335 , 1990
Cells
Heart
Adrenal chromaffin
Paraneurons
Enzymes
Trypsin : 0.06%
Medium
25mM HEPES buffered Locke's solution, CMF
Adrenal
Rat
Rat
Englert, D.F.
An Optical Study of Isolated Rat Adrenal Chromaffin Cells ,
Exp Cell Res
 125 , 369 , 1980
Cells
Chromaffin
Enzymes
Trypsin : 0.10%
Medium
Ham's F-12 w/HEPES
Adrenal
Rat
Rat, Wistar-Hanover, 7-12 day
Unsicker, K. , Krisch, B. , Otten, U. and Thoenen, H.
Nerve Growth Factor-Induced Fiber Outgrowth From Isolated Rat Adrenal Chromaffin Cells: Impairment by Glucocorticoids ,
Proc Natl Acad Sci U S A
 75 (7) , 3498 , 1978
Cells
Medullary
Enzymes
Trypsin : 0.125%
Medium
HBSS
Adrenal
Rat
Rat, SD, male
Barofsky, A. , Feinstein, M. and Halkerston, I.
Enzymatic and Mechanical Requirements for the Dissociation of Cortical Cells From Rat Adrenal Glands ,
Exp Cell Res
 79 , 263 , 1973
Cells
Cortical
Enzymes
Trypsin : 0.25%
Medium
Kreb's Ringer bicarbonate buffer
Bone
Human
Human
Taylor, S. , Shah, M. and Orriss, I.
Generation of Rodent and Human Osteoblasts. ,
Bonekey Rep
 3 , 585 , 2014
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%-1.0%
Medium
DMEM
Bone
Human
Human, 22-73 year
Kneser U, Voogd A, Ohnolz J, Buettner O, Stangenberg L, Zhang YH, Stark GB, Schaefer DJ.
Fibrin gel-immobilized primary osteoblasts in calcium phosphate bone cement: in vivo evaluation with regard to application as injectable biological bone substitute ,
Cells Tissues Organs
 179 , 158-69 , 2005
Cells
Osteoblasts
Enzymes
Trypsin : 0.5%
Medium
Basal Medium
Bone
Human
Human
Meikle, M. , Boyd, S. , Hembry, R. , Compston, J. , Croucher, P. and Reynolds, J.
Human Osteoblasts in Culture Synthesize Collagenase and Other Matrix Metalloproteinases in Response to Osteotropic Hormones and Cytokines ,
J Cell Sci
 103 , 1093 , 1992
Cells
Osteoblasts
Enzymes
Trypsin : 0.1%
Medium
DMEM
Bone
Rat
Rat, 2 day
Jeon, J. , Lee, M. and Yang, H.
Differentiated Osteoblasts Derived Decellularized Extracellular Matrix to Promote Osteogenic Differentiation. ,
Biomater Res
 22 , 4 , 2018
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%
Medium
DMEM
Bone
Rat
Rat, 2-3 day
Taylor, S. , Shah, M. and Orriss, I.
Generation of Rodent and Human Osteoblasts. ,
Bonekey Rep
 3 , 585 , 2014
Cells
Osteoblasts
Enzymes
Trypsin : 0.25%-1.0%
Medium
DMEM
Brain
Human
Human
Mizee, M. , Miedema, S. , van der Poel, M. , Adelia , S. , van Strien, M. , Melief, J. , Smolders, J. , Hendrickx, D. , Heutinck, K. , Hamann, J. and Huitinga, I.
Isolation of Primary Microglia from the Human Post-Mortem Brain: Effects of Ante- and Post-Mortem Variables. ,
Acta Neuropathol
 5 , 16 , 2017
Cells
Microglia
Enzymes
Collagenase Type 1 : 300 u/ml
Trypsin : 0.125%
Medium
DMEM
Brain
Human
Human
Klegeris Andis, McGeer PatrickL.
Chymotrypsin-like proteases contribute to human monocytic THP-1 cell as well as human microglial neurotoxicity ,
Glia
 51 , 56-64 , 2005
Cells
Microglia
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Brain
Rat
Rat, SD
Akanda, N. , Tofighi, R. , Brask, J. , Tamm, C. , Elinder, F. and Ceccatelli, S.
Voltage-Dependent Anion Channels (VDAC) in the Plasma Membrane Play a Critical Role in Apoptosis in Differentiated Hippocampal Neurons but not in Neural Stem Cells. ,
Cell Cycle
 7 , 3225 , 2008
Cells
Hippocampal
Enzymes
Trypsin : 0.1%
Medium
Neurobasal
Brain
Rat
Rat, Wistar, 1-3 day
Velasco Myrian, Garci­a Esperanza, Onetti Carlos G.
Glucose deprivation activates diversity of potassium channels in cultured rat hippocampal neurons ,
Cell Mol Neurobiol
 26 , 307-19 , 2006
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.05%
Medium
DMEM
Brain
Rat
Rat, newborn
Holzwarth, J. , Glaum, S. and Miller, R.
Activation of Endothelin Receptors by Sarafotoxin Regulates Ca2+ Homeostasis in Cerebellar Astrocytes ,
Glia
 5 , 239 , 1992
Cells
Astrocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM, HBSS
Brain
Rat
Rat, SD, newborn
Pixley, S.K.
The Olfactory Nerve Contains Two Populations of Glia ,
In Vitro
 5 , 269 , 1992
Cells
Glial
Enzymes
Trypsin : 0.0625%
Medium
MEM, sterile
Brain
Rat
Rat, 60-72 hours old
Acheson, A. , Barker, P. , Alderson, R. , Miller, F. and Murphy, R.
Detection of Brain-Derived Neurotrophic Factor-like Activity in Fibroblasts and Schwann Cells: Inhibition by Antibodies to NGF ,
Neuron
 7 , 265 , 1991
Cells
Fibroblasts
Enzymes
Trypsin : 0.2%
Medium
HEPES buffered DMEM
Brain
Rat
Rat, SD, 5 day
Mattson, M. and Kater, S.
Development and Selective Neurodegeneration in Cell Cultures From Different Hippocampal Regions ,
Brain Res
 490 , 110 , 1989
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.2%
Deoxyribonuclease I : 10 ug/ml
Medium
HBSS
Brain
Rat
Rat, albino, adult and newborn
Giulian, D. and Baker, T.J.
Characterization of Ameboid Microglia Isolated From Developing Mammalian Brain ,
J Neurosci
 6 , 2163 , 1986
Cells
Cerebral cortices
Enzymes
Trypsin : 0.25%
Medium
PBS
Brain
Rat
Rat, Wistar, newborn
Goldman, J.E. , Geier, S.S. and Hirano, M.
Differentiation of Astrocytes and Oligodendrocytes From Germinal Matrix Cells in Primary Culture ,
J Neurosci
 6 , 52 , 1986
Cells
Germinal matrix
Enzymes
Trypsin : 0.25%
Medium
HBSS
Brain
Rat
Rat, fetus, 18 day
Rothman, S.
Synaptic Release of Excitatory Amino Acid Neurotransmitter Mediates Anoxic Neuronal Death ,
J Neurosci
 4 (7) , 1884 , 1984
Cells
Hippocampi
Enzymes
Trypsin : 0.1%
Medium
HBSS
Brain
Rat
Rat, fetus, 18-20 day
Bartlett, W. and Banker, G.
An Electron Microscopic Study of the Development of Axons and Dendrites by Hippocampal Neurons in Culture. I. Cells Which Develop Without Intercellular Contacts ,
J Neurosci
 4 , 1944 , 1984
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.25%
Medium
HBSS, CMF
Brain
Rat
Rat, SD, 19-20 days pregnant
Ahmed, Z. , Walker, P. and Fellows, R.
Properties of Neurons from Dissociated Fetal Rat Brain in Serum-Free Culture ,
J Neurosci
 3 (12) , 2448 , 1983
Cells
Neural
Enzymes
Trypsin : 0.25%
Medium
DMEM
Brain
Rat
Rat, fetus
Swaiman, K. , Neale, E. , Fitzgerald, S. and Nelson, P.
A Method for Large-scale Production of Mouse Brain Cortical Cultures ,
Brain Res
 255 , 361 , 1982
Cells
Cortical
Enzymes
Trypsin : 0.25%
Medium
Puck's D1 (see reference)
Brain
Rat
Rat, Wistar, pregnant
Abney, E. , Bartlett, P. and Raff, M.
Astrocytes, Ependymal Cells, and Oligodendrocytes Develop on Schedule in Dissociated Cell Cultures of Embryonic Rat Brain ,
Dev Biol
 83 , 301 , 1981
Cells
Glial
Enzymes
Trypsin : 0.05%
Medium
Eagle's MEM/DMEM
Brain
Rat
Rat, Wistar, adult, 170 g
Phillips, P. , Kumar, P. , Kumar, S. and Waghe, M.
Isolation And Characterization of Endothelial Cells From Rat And Cow Brain White Matter ,
J Anat
 129 , 261 , 1979
Cells
Endothelial
Enzymes
Trypsin : 0.5%
Medium
BSS
Brain
Rat
Rat, Holtzmann, 18 day
Banker, G. and Cowan, M.
Rat Hippocampal Neurons in Dispersed Cell Culture ,
Brain Res
 126 , 397 , 1977
Cells
Hippocampal neurons
Enzymes
Trypsin : 0.1%
Medium
HBSS
Brain
Rat
Rat, SD, 2 days
Rowe, V. , Neale, E. , Avins, L. , Guroff, G. and Schrier, B.
Pineal gland cells in culture. Morphology, Biochemistry, Differentiation, and co-culture with sympathetic neurons ,
Exp Cell Res
 104 , 345 , 1977
Cells
Pineal
Enzymes
Trypsin : 0.25%
Medium
DMEM, MEM
Cartilage
Human
Human
Muraglia, A. , Nguyen, V. , Nardini, M. , Mogni, M. , Coviello, D. , Dozin, B. , Strada, P. , Baldelli, I. , Formica, M. , Cancedda, R. and Mastrogiacomo, M.
Culture Medium Supplements Derived from Human Platelet and Plasma: Cell Commitment and Proliferation Support. ,
Front Bioeng Biotechnol
 5 , 66 , 2017
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Hyaluronidase : 0.1%
Collagenase Type 2 : 1000 u/ml
Collagenase Type 1 : 400 u/ml
Medium
Ham's F12
Cartilage
Human
Human w/ rheumatoid arthritis
Dayer, J. , Krane, S. , Russell, R. and Robinson, D.
Production of Collagenase and Prostaglandins by Isolated Adherent Rheumatoid Synovial Cells ,
Proc Natl Acad Sci U S A
 73 (3) , 945 , 1976
Cells
Synovial tissue
Enzymes
Trypsin : 0.05%
Medium
DMEM
(see reference)
Cartilage
Human
Human, 13-62 years
Srivastava, V.M.L. , Malemud, C.J. , Hough, A.J. , Bland, J.H. and Sokoloff, L.
Preliminary Experience with Cell Culture of Human Articular Chondrocytes ,
Arthritis Rheum
 17 , 165 , 1974
Cells
Articular chondrocytes
Enzymes
Trypsin : 0.2%
Medium
BSS
Cartilage
Rat
Rat
Mei, L. , Shen, B. , Ling, P. , Liu, S. , Xue, J. , Liu, F. , Shao, H. , Chen, J. , Ma, A. and Liu, X.
Culture-expanded Allogenic Adipose Tissue-derived Stem Cells Attenuate Cartilage Degeneration in an Experimental Rat Osteoarthritis Model. ,
PLoS ONE
 12 , e0176107 , 2017
Cells
Chondrocytes
Enzymes
Trypsin : 0.25%
Medium
DMEM/F12
Cartilage
Rat
Rat, SD, young, 100-120 g
Shimomura, Y. , Yoneda, T. and Suzuki, F.
Osteogenesis by Chondrocytes from Growth Cartilage of Rat Rib ,
Calcif Tissue Res
 19 , 179 , 1975
Cells
Chondrocytes
Enzymes
Trypsin : 0.2%
Medium
Ham's F-12 medium
Colon
Human
Human
Ali, M. , Anand, S. , Tangella, K. , Ramkumar, D. and Saif, T.
Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry. ,
J Vis Exp
 , e52532 , 2015
Cells
Colon
Enzymes
Trypsin : 0.1%
Collagenase : 0.1%
Medium
HBSS
Colon
Human
Human
Raghupathi, R. , Duffield, M. , Zelkas, L. , Meedeniya, A. , Brookes, S. , Sia, T. , Wattchow, D. , Spencer, N. and Keating, D.
Identification of Unique Release Kinetics of Serotonin From Guinea-pig and Human Enterochromaffin Cells. ,
J Physiol
 591 , 5959-75 , 2013
Cells
Enterochromaffin
Enzymes
Trypsin : 0.05%
Medium
DMEM

Tissue Tables (references, grouped by tissue type and species)

Adipose/Fat
Adrenal
Bone
Brain
Cartilage
Colon
Endothelial
Epithelial
Eye
Heart
Intestine
Kidney
Liver
Lung
Lymph nodes
Mammary
Miscellaneous
Muscle
Neural
Organoids
Pancreas
Parotid
Pituitary
Prostate
Reproductive
Scales
Skin
Spleen
Stem
Thymus
Thyroid/Parathyroid
Tonsil
Tumor
Note: We have not limited the references listed to only those papers using Worthington enzymes. Generally speaking, the tissue dissociation enzymes offered by Worthington can be used interchangeably for most preparations cited.